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BACKGROUND MET fusion is a key driver mutation, but it is rare in gastric cancer (GC). Several MET (hepatocyte growth factor receptor) inhibitors have been approved for the treatment of MET-positive patients, but the tumor response is heterogeneous. With the development of next-generation sequencing, diverse MET fusion partner genes have been identified. We herein report a fusion variant involving KIF5B-MET in GC. CASE SUMMARY After thoracoscopic inferior lobectomy plus lymph node dissection under general anesthesia, a "tumor within a tumor" was found in the lung tumor tissue of a 64-year-old non-smoking male patient. Combining the medical history and the results of enzyme labeling, the focal area was considered to be GC. To seek potential therapeutic regimens, an intergenic region between KIF5B and MET fusion was identified. This fusion contains a MET kinase domain and coil-coiled domains encoded by KIF5B exons 1-25, which might drive the oncogenesis. CONCLUSION Our finding could extend the spectrum and genomic landscape of MET fusions in GC and favor the development of personalized therapy.

To explore the effectiveness of magnetic resonance image (MRI)-based biomarkers for identifying benign and malignant parotid tumors via diagnostic model analysis. This retrospective study included 109 patients (development cohort and validation cohort) who underwent MRI preoperatively, including T1- and T2-weighted images. Parameters based on 2D or 3D texture analysis were extracted from tumor lesions by MaZda software, fisher discriminant and bootstrap method were used to perform parameter reduction, diagnostic models with the selected biomarkers were established along with clinical data, model performance (discrimination and calibration) was furtherly evaluated by internal and external validation, decision curve analysis was applied to measure the improvement of clinical benefits. S(5,5) Entrop, S(0,1) ASM, WavEnHH (s-4), S(1,1,0) Entropy and Perc.10% were significantly associated with the pathological diagnosis of parotid tumor (benign versus malignancy), when adding these biomarkers to the regression analysis, model performance significantly improved in the development cohort (likelihood-ratio-test; p

The objective of this study was to compare the diagnostic performance of magnetic resonance imaging (MRI) and computed tomography (CT) in differentiating pleomorphic adenomas from Warthin tumors using radiomics.We retrospectively reviewed 626 patients who underwent preoperative MRI or CT for parotid tumor diagnosis. Patient groups were balanced by propensity score matching (PSM) and 123 radiomic features were extracted from tumor images. Radiomic signatures (rad-scores) were generated using a least absolute shrinkage and selection operator logistic regression model. The Canny edge detector was used to define tumor borders (border index). The diagnostic performance of rad-score and border index before and after PSM was evaluated with area under the receiver operating characteristic curve analysis.For differentiation of pleomorphic adenomas and Warthin tumors, rad-score and border index areas under the curve for MRI after PSM were 0.911 (95% confidence interval [CI], 0.871-0.951) and 0.716 (95% CI, 0.646-0.787), respectively; those for CT were 0.876 (95% CI, 0.829-0.923) and 0.608 (95% CI, 0.527-0.690), respectively. Tumor border index on MRI, but not CT, had superior diagnostic performance (P.05); MRI- and CT-based rad-scores showed similar performance (P.05).MRI is superior to CT for tumor margin examination; however, the radiomics features of both modalities showed no difference.

Li Hua Cui,1 Hai Rong Xu,2 Wu Yang,1 Li Jiang Yu1 1Department of Oncology, People’s Hospital of Jingjiang, Jingjiang, China; 2Department of Oncology, The Northern Jiangsu People’s Hospital, Yangzhou, China Background: Investigating the roles of lncRNA prostate cancer-associated transcript 6 (PCAT6) in modulating the growth and aggressiveness of non-small-cell lung carcinoma (NSCLC) cell.Method: The levels of PCAT6 in NSCLC tissues and cell lines were determined by quantitative real-time PCR assay. MTT as well as colony formation assays were applied to explore the effect of PCAT6 on the growth of NSCLC cell in vitro. Wound healing and Transwell assays were utilized to analyze the impact of PCAT6 on the migration and invasion of NSCLC cell. Bioinformatics analysis and luciferase reporter assay were used to prove that miR-330-5p was the target of PCAT6. Colony formation, wound healing, and Transwell invasion assays were applied to demonstrate that PCAT6 promoted NSCLC cell growth, migration, and invasion through binding miR-330-5p. Finally, xenograft model was used to explore the role of PCAT6 in the tumor growth of NSCLC cell in vivo.Results: PCAT6 was highly overexpressed in NSCLC tissues and cells compared with normal tissues and non-tumorigenic bronchial epithelial cell line, BEAS-2B. Downregulation of PCAT6 markedly reduced the proliferation, migration, and invasion of NSCLC cell. Moreover, down-expression of PCAT6 significantly increased the level of miR-330-5p in NSCLC cell. Further functional experiments indicated that down-expression of miR-330-5p reversed the inhibitory effect of PCAT6 on NSCLC cell growth, migration, and invasion.Conclusion: Our results reveal that lncRNA PCAT6 facilitates the proliferation, migration, and invasion of NSCLC cell via competitively binding to miR-330-5p. Keywords: PCAT6, MiR-330-5p, NSCLC, growth, migration, invasion

Oral squamous cell carcinoma (OSCC) is a highly invasive lesion that frequently metastasizes to the cervical lymph nodes and is associated with a poor prognosis. Several adhesion factors, including cadherin 6 (CDH6), cadherin 11 (CDH11) and cluster of differentiation 44 (CD44), have been reported to be involved in the invasion and metastasis of multiple types of cancer. Therefore, the aim of the present study was to determine the expression of CDH6, CDH11 and CD44 in tumor tissues from patients with OSCC, and whether this was associated with the metastasis and survival of OSCC. The mRNA expression of the human tumor metastasis-related cytokines was examined by reverse transcription-quantitative polymerase chain reaction (RT-qPCR) in OSCC tumors with or without lymph node metastasis (n=10/group). The expression of CDH6, CDH11 and CD44 in 101 OSCC and 10 normal oral mucosa samples was examined by immunohistochemical staining. The association between overall and disease-specific survival times of patients with OSCC and the expression of these three proteins was evaluated using Kaplan-Meier curves and the log-rank test. RT-qPCR results indicated that the mRNA expression of CDH6, CDH11 and CD44 was increased in OSCC patients with lymph node metastasis (2.93-, 2.01- and 1.92-fold; P

Pathological scars,including keloids and hypertrophic scars,result from aberrations in the process of physiologic wound healing.An exaggerated inflammatory process is one of the main pathophysiological contributors.Pathological scars may cause pain and pruritis,limit joint mobility,and cause a range of cosmetic deformities that affect the patient's quality of life.However,the effectiveness of currently available prevention and treatment measures remains unsatisfactory.Mesenchymal stem cells,among their multifunctional roles,have the functions of immunomodulation and promotion of angiogenesis.Thus,they have been proposed to be a major candidate for cell therapy to treat or prevent pathologicalscars.This article reviews the mechanism and potentials of stem cell therapy in the prevention and treatment of pathological scars.病理性瘢痕,包括增生性瘢痕和瘢痕疙瘩,是皮肤创伤后组织异常修复的结果。炎症反应是促进病理性瘢痕形成的主要原因。病理性瘢痕常引起瘙痒、疼痛、关节活动受限和畸形,进而影响到患者的生活质量。目前常用的治疗方法效果并不理想。间充质干细胞因具有多向分化潜能、免疫调节及促血管形成作用,可影响瘢痕的形成及发展,作为一种细胞治疗方法有望用来预防及治疗病理性瘢痕,已成为近期研究热点。本文主要综述间充质干细胞预防及治疗病理性瘢痕机制方面的最新进展。.

Since 1980, the white-browed crake (Porzana cinerea) has been experiencing an expansion from south of the Isthmus of Kra, northward to China. Recently, this species was observed in several locations throughout Southwest China, including Ningming and Baise, Guangxi (2012, 2013), and Xichang, Sichuan (2013). These sightings are the first distribution record of this species in mainland China, suggesting that the white-browed crake is following a natural species dispersal northward into mainland China from Southeast Asia.

T0 understand the population distribution, species abundance and habitats requirement of a medicinal animal, Centropus sinensis, in Guangxi Province.Line transect methods were used to census population number and habitat types in 18 counties or cities of Guangxi.Centropus sinensis distributed in all study area, with a higher population density in the south than in the north. Among seven habitat types, the species preferred living in forest-farmland ecotone, while seldom in broad-leaves forest or plantations.The results of habitat preference show an advantage to the population recovering for forest-farmland ecotone commonly existing in Guangxi Province. But the speed of population recovering is slowly under illegal hunting pressure. More attention should be paid to protect this species.

Since 1980, the white-browed crake (Porzana cinerea) has been experiencing an expansion from south of the Isthmus of Kra, northward to China. Recently, this species was observed in several locations throughout Southwest China, including Ningming and Baise, Guangxi (2012, 2013), and Xichang, Sichuan (2013). These sightings are the first distribution record of this species in mainland China, suggesting that the white-browed crake is following a natural species dispersal northward into mainland China from Southeast Asia.

To discuss the feasibility of using free tissue flap for simultaneous tongue reconstruction after tongue cancer resection.Radial forearm flaps and anterolateral thigh flaps were used for simultaneous reconstruction of different defects after tongue cancer resection in 47 cases. The functional evaluation was assessed during the follow-up period of 3 months to 9 years.Only one flap failed,giving a success rate of 97.7%. The 36 patient available for postoperative follow-up were all able to communicate in basic languages and eat normally. Among them, normal speech was found in 34, while slurred speech was found in two. 31 patients could eat normal diet ,while eight could eat soft diet and two could eat liquid diet.Simultaneous tongue reconstruction with free tissue flap is a reliable method with high successful rate. Flaps selection based on different tongue defects is the key point to achieve good functional and cosmetic results for the reconstruction tongue.

To explore the reconstruction method of large through-and-through palate defects.From 2003 to 2006, 7 cases of large through-and-through palate defects were reconstructed with vascularized folded free forearm flap. 8 flaps were used, including 7 free forearm flaps and 1 pectoralis major myocutaneous flap.All the tissue flaps survived well except one flap necrosis because of arterial thrombosis. The appearance of reconstructed palate was acceptable, and the functions of swallowing and speech were normal or almost normal.It is feasible and effective to repair large through-and-through palate defects with folded free forearm flap.