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2. Molecular characterization and biofilm formation ability of Enterococcus faeciumand Enterococcus faecalisbloodstream isolates from a Chinese tertiary hospital in Beijing

Author

Yang, Jing-xian, Liu, Cun-wei, Wu, Fu-wei, Zhu, Ling, and Liang, Guo-wei

Abstract

To investigate the molecular characteristics and biofilm-forming ability of 116 Enterococcus faecium(Efm) and 72 Enterococcus faecalis(Efs) isolates obtained from patients with bloodstream infections (BSI) at a Chinese hospital between July 2011 and March 2018. The presence of glycopeptide resistance genes and five virulence genes (esp, gelE, asa1, hyl, and cylA) was screened using two multiplex PCR. MLST was used to assess the clonality. Crystal violet staining was used to detect biofilms. Vancomycin resistance was detected in 30.1% of Efmand 2.8% of Efsisolates, respectively. All VRE strains carried the vanAgene. The esp, gelE, asa1, and cylAgenes in 72 Efsstrains were detected at 62.5%, 84.7%, 84.7%, and 69.4%, respectively. Among the 116 Efmisolates, 74.1% and 25.8% carried espand hyl, respectively. The espgene was significantly associated with vancomycin-resistant Efm(VREfm) compared to vancomycin-susceptible Efm(VSEfm). In total, 91.7% of Efsand 20.0% of Efmproduced biofilms. Twenty-six STs were identified among the 72 Efsisolates, with ST4 (29.2%) being the predominant. In total, 116 Efmstrains were grouped into 26 STs, with ST78 (46.6%) being the predominant. Both VREfm (41.7%) and VSEfm (48.8%) were dominant in ST78. There is no clear evidence suggesting that some STs are associated with vancomycin resistance or biofilm formation. Both Efmand EfsBSI isolates showed a polyclonal pattern with a dominant clone and many unique types, implying the coexistence of clonal dissemination and an influx of new clones. The horizontal transmission of resistance genes may play a more important role in VREfm prevalence than clonal expansion.

Published
2024
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5. Species Distribution and Antifungal Susceptibility of Invasive Candidiasis: A 2016-2017 Multicenter Surveillance Study in Beijing, China

Author

Guo, Li-Na, Yu, Shu-Ying, Xiao, Meng, Yang, Chun-Xia, Bao, Chun-Mei, Yu, Yan-Hua, Ye, Li-Yan, Yang, Yang, Zhang, Ge, Liu, Jie, Liang, Guo-Wei, Min, Rong, Zhu, Yu, Lei, Hong, Liu, Yu-Lei, Liu, Lin-Juan, Hu, Yun-Jian, Hsueh, Po-Ren, and Xu, Ying-Chun

Subjects
invasive fungal infections, Infection and Drug Resistance, Beijing, surveillance, species distribution, antifungal resistance, Original Research, Candida
Abstract

Li-Na Guo,1,2 Shu-Ying Yu,1– 3 Meng Xiao,1,2 Chun-Xia Yang,4 Chun-Mei Bao,5 Yan-Hua Yu,6 Li-Yan Ye,7 Yang Yang,1,2 Ge Zhang,1,2 Jie Liu,8 Guo-Wei Liang,9 Rong Min,10 Yu Zhu,11 Hong Lei,12 Yu-Lei Liu,13 Lin-Juan Liu,14 Yun-Jian Hu,15 Po-Ren Hsueh,16 Ying-Chun Xu1,2 1Department of Clinical Laboratory, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences; 2Beijing Key Laboratory for Mechanisms Research and Precision Diagnosis of Invasive Fungal Diseases (BZ0447); 3Graduate School, Peking Union Medical College, Chinese Academy of Medical Sciences; 4Department of Infectious Diseases & Clinical Microbiology, Beijing Chao-Yang Hospital, Capital Medical University; 5Clinical Laboratory Medical Center, The Fifth Medical Center of Chinese PLA General Hospital; 6Department of Clinical Laboratory, You’an Hospital, Capital Medical University; 7Center for Clinical Laboratory Medicine, Chinese PLA General Hospital; 8Department of Clinical Laboratory, Seventh Medical Center, PLA General Hospital; 9Department of Clinical Laboratory, Aerospace Center Hospital; 10Department of Clinical Laboratory, Xuanwu Hospital, Capital Medical University; 11Department of Clinical Laboratory, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College; 12Department of Clinical Laboratory, The 8th Medical Center of Chinese PLA General Hospital; 13Department of Clinical Laboratory, Beijing Anzhen Hospital, Capital Medical University; 14Department of Clinical Laboratory, Peking University Cancer Hospital and Institute; 15Department of Clinical Laboratory, Beijing Hospital, Beijing, People’s Republic of China; 16Departments of Laboratory Medicine & Internal Medicine, National Taiwan University Hospital, National Taiwan University College of Medicine, Taipei, TaiwanCorrespondence Ying-Chun XuDepartment of Clinical Laboratory, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, No. 1, Shuaifuyuan Wangfujing, Dongcheng District, Beijing 100730, People’s Republic of ChinaTel/ Fax +86 106 915 9766Email xycpumch@139.comPo-Ren HsuehDepartments of Laboratory Medicine & Internal Medicine, National Taiwan University Hospital, National Taiwan University College of Medicine, Taipei, TaiwanTel +886-2-23224262 ext. 65355Fax +886-2-23955072Email hsporen@ntu.edu.twObjective: Invasive candidiasis (IC), a life-threatening fungal infection prevalent among hospitalized patients, has highly variable regional epidemiology. We conducted a multicenter surveillance study to investigate recent trends in species distribution and antifungal susceptibility patterns among IC-associated Candida spp. in Beijing, China, from 2016 to 2017.Materials and Methods: A total of 1496 non-duplicate Candida isolates, recovered from blood and other sterile body fluids of IC patients, were identified using matrix-assisted laser desorption/ionization time of flight mass spectrometry combined with ribosomal DNA internal transcribed spacer (ITS) region sequencing. Broth microdilution-based susceptibility testing using six antifungal agents was also conducted.Results: Candida albicans was the most frequently isolated species (49.9%), followed by Candida tropicalis (15.5%), Candida glabrata (14.7%) and Candida parapsilosis (14.2%). No significant differences in species distribution were observed when compared with a 2012– 2013 dataset. Overall, the rates of susceptibility to fluconazole and voriconazole were high among C. albicans (98% and 97.2%, respectively) and C. parapsilosis species complex (91.1% and 92%, respectively) isolates but low among C. tropicalis (81.5% and 81.1%, respectively) isolates. In addition, the rate of azole resistance among C. tropicalis isolates increased significantly (1.8-fold, P< 0.05) compared with that observed in 2012– 2013, while micafungin resistance rates were < 5% for all tested Candida species.Conclusion: Our results suggest that species distribution has remained stable among IC-associated Candida isolates in Beijing. Resistance to micafungin was rare, but increased azole resistance among C. tropicalis isolates was noted. Our study provides information on local epidemiology that will be important for the selection of empirical antifungal agents and contributes to global assessments of antifungal resistance.Keywords: invasive fungal infections, Candida, species distribution, antifungal resistance, surveillance, Beijing

Published
2020

6. The tcdA-negative and tcdB-positive Clostridium difficile ST81 clone exhibits a high level of resistance to fluoroquinolones: a multi-centre study in Beijing, China

Author

Cheng, Jing-Wei, primary, Liu, Chang, additional, Kudinha, Timothy, additional, Xiao, Meng, additional, Fan, Xin, additional, Yang, Chun-Xia, additional, Wei, Ming, additional, Liang, Guo-Wei, additional, Shao, Dong-Hua, additional, Xiong, Zhu-Jia, additional, Hou, Xin, additional, Yu, Shu-Ying, additional, Wang, Yao, additional, Yang, Qi-Wen, additional, Su, Jian-Rong, additional, and Xu, Ying-Chun, additional

Published
2020
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9. Epidemiology and antifungal susceptibilities of yeast isolates causing invasive infections across urban Beijing, China

Author

Guo, Li-Na, primary, Xiao, Meng, additional, Cao, Bin, additional, Qu, Fen, additional, Zhan, Yu-Liang, additional, Hu, Yun-Jian, additional, Wang, Xin-Ru, additional, Liang, Guo-Wei, additional, Gu, Hai-Tong, additional, Qi, Jun, additional, Yuan, Hui, additional, Min, Rong, additional, Wang, Fei-Yan, additional, Liu, Lin-Juan, additional, Wang, Hai-Bin, additional, Jiang, Wei, additional, Duan, Xue-Guang, additional, Xu, Wen-Jian, additional, Yu, Yan-Hua, additional, Su, Jian-Rong, additional, Zhang, Jian-Zhong, additional, Nong, Jin-Qing, additional, Liu, Shu-Mei, additional, Li, Jun, additional, Liu, Jun-Ting, additional, Yue, Zhi-Gang, additional, Yang, Duo, additional, Guo, Jie, additional, Zhao, Rui, additional, Zhang, Ya-Nan, additional, Yang, Xi-Ming, additional, Liu, Xiao-Qing, additional, Hsueh, Po-Ren, additional, and Xu, Ying-Chun, additional

Published
2017
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11. Experimental study and computational simulation on a new kind of differential pressure flow-sensor

Author

Liang Guo-wei, Shen Ping-ping, and Ge Yun-yan

Subjects
Engineering, business.industry, Mechanics, Pressure sensor, law.invention, Metrology, Physics::Fluid Dynamics, Computational simulation, Pressure measurement, Flow (mathematics), law, Range (statistics), Fluent, Two-phase flow, business, Simulation
Abstract

A new kind of differential pressure flow-sensor consisted of double-cones was developed. The relationship curve of flow-rate and differential pressure of the flow-sensor was obtained by means of experimental study and FLUENT computational simulation. The results indicated that the design of the flow-sensor possesses the lower pressure loss, more steady flow and wider flow range than the conventional differential pressure flow-meter. It is prospective to applying to the measurement of gas-liquid two-phase flow area.

Published
2010

20. Urinary Myeloperoxidase to Creatinine Ratio as a New Marker for Diagnosis of Urinary Tract Infection.

Author

Bai MJ, Feng J, and Liang GW

Subjects
Aged, Biomarkers urine, Female, Humans, Male, Middle Aged, ROC Curve, Urinary Tract Infections microbiology, Creatinine urine, Peroxidase urine, Urinary Tract Infections diagnosis, Urinary Tract Infections urine
Abstract

Objective To determine whether urinary myeloperoxidase to creatinine ratio (MCR) can serve as a marker for diagnosis of urinary tract infection (UTI).Methods Patients suspected of UTI were consecutively enrolled and further divided into the culture positive and the sterile groups according to urine culture results. Subsequently, MCR, white blood cell (WBC) and bacteria in the urinary samples from patients were detected and compared between the two groups.Results Finally, 253 patients were enrolled including 157 urine culture positive patients and 96 urine culture negative patients (sterile group). After logarithmic transformation in 2 as the base, the MCR, WBC, and bacteria were separately presented as log 2 MCR , log 2 WBC (quantitative) , and log 2 bacteria . The values of log 2 MCR (8.6±2.5 vs. 5.4±1.5, t=-12.453, P=0.001), log 2 WBC (quantitative) (8.0±2.5 vs. 5.2±1.8, t=-10.332, P=0.001), log 2 bacteria (11.4±2.5 vs. 8.2±2.8, t=-9.297, P=0.001) and WBC (semi-quantitative) [2 (interquartile range 1, 3) vs. 1 (interquartile range 0.5, 1), Z=-7.580, P=0.001] showed significant difference between the urine culture positive group and the sterile group. Among the urine culture positive group, the values of log 2 MCR of the gram positive and gram negative subgroups were 7.2±2.5 and 9.0±2.4 (t=4.016, P=0.001), respectively. The correlation between log 2 MCR and log 2 WBC (quantitative), log 2 bacteria , WBC (semi-quantitative) was 0.708 (Pearson correlation, P=0.001), 0.381 (Pearson correlation, P=0.001), and 0.606 (Spearman correlation, P=0.001), respectively. Conclusions MCR is positively correlated with WBC counts and could be served as a promising biomarker for diagnosis of UTI. MCR could be even used for initial inference of infectious bacteria types of UTI.

Published
2018
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21. [A novel modi cation of real-time AS-qPCR by using locked nucleic acid-modified oligonucleotide probe as wild type allele amplification blockers for quantitative detection of the JAK2 V617F mutation].

Author

Shao DH, Liang GW, He ML, and Cao QY

Subjects
Alleles, Humans, Oligonucleotide Probes genetics, Oligonucleotides genetics, Sensitivity and Specificity, Janus Kinase 2 genetics, Mutation, Real-Time Polymerase Chain Reaction methods
Abstract

Objective: To develop a novel real-time PCR for sensitively quantitative detection of JAK2 V617F allele burden in peripheral blood., Methods: Based on the real-time allele-specific PCR (AS-qPCR), the locked nucleic acid (LNA)-modified oligonucleotide probe was used for selectively blocking amplification of wild-type alleles in AS-qPCR, and then a novel AS-LNA-qPCR method was established. The percentages of sample JAK2 V617F alleles were directly calculated by its threshold cycle (Ct) values according to the standard curve which generated by JAK2 V617F alleles with its Ct values. We validated intra- and inter-assay variability for quantifying JAK2 V617F. We also assayed 623 apparent healthy donors by our method to validate its clinical application value., Results: The quantitative lower limit of this method for JAK2 V617F was 0.01%, and the intra- and inter-assay average variability for quantifying percentage of JAK2 V617F in total DNA was 6.3% and 8.6%, respectively. Nineteen JAK2 V617F-positive individuals were identified using AS-LNA-qPCR in blood of 623 apparently healthy donors, and the range of percentages of JAK2 V617F alleles were 0.01%-5.49%., Conclusion: The AS-LNA-qPCR with highly sensitive and reproducible quantification of JAK2 V617F mutant burden can be used clinically for diagnosis as well as evaluation of disease prognosis and efficacy of therapy in patients with myeloproliferative neoplasms.

Published
2013
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22. [Degradation of p-nitrophenol by high voltage pulsed discharge and ozone processes].

Author

Pan LL, Yan GQ, Zheng FY, Liang GW, and Fu JJ

Subjects
Biodegradation, Environmental, Bioreactors, Hydrogen-Ion Concentration, Oxidation-Reduction, Electrochemistry, Industrial Waste analysis, Nitrophenols analysis, Ozone chemistry, Waste Disposal, Fluid methods
Abstract

The vigorous oxidation by ozone and the high energy by pulsed discharge are utilized to degrade the big hazardous molecules. And these big hazardous molecules become small and less hazardous by this process in order to improve the biodegradability. When pH value is 8-9, the concentration of p-nitrophenol solution can be degraded by 96.8% and the degradation efficiency of TOC is 38.6% by ozone and pulsed discharge treatment for 30 mins. The comparison results show that the combination treatment efficiency is higher than the separate, so the combination of ozone and pulsed discharge has high synergism. It is approved that the phenyl degradation efficiency is high and the degradation efficiency of linear molecules is relative low.

Published
2005

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