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Background Sarcopenia is characterised by loss of muscle strength and mass, leading to falls and adverse health outcomes. Our aim was to determine the prevalence of sarcopenia in patients with axial spondyloarthritis (ax-SpA) and to identify factors associated with sarcopenia in these patients. Methods This cross-sectional study included 120 male patients with ax-SpA and 100 healthy individuals matched for both sex and age. Sarcopenia was assessed by muscle strength, muscle mass and physical performance using the criteria determined by the Asian Working Group for Sarcopenia (AWGS). The ankylosing spondylitis disease activity score (ASDAS), laboratory indicators and bone mineral density were determined. Falls/fractures sustained over the prior year were evaluated. The association between sarcopenia and clinical characteristics was evaluated using logistic regression analyses. Results According to the diagnostic criteria of AWGS 2019, the prevalence of sarcopenia, severe sarcopenia and possible sarcopenia was 20.8%, 9.2 and 16.7% higher than in the healthy control population (p Conclusions Patients with ax-SpA have a high incidence of sarcopenia. The development of sarcopenia was associated with high ASDAS, reduced levels of prealbumin and 25-(OH)D as well as the incidence of falls/fractures.

Endothelial dysfunction is a critical mediator in the pathogenesis of vascular complications of diabetes. Herein, this study was conducted to investigate the therapeutic effects of Salvianolic acid B (Sal B) on diabetes-induced endothelial dysfunction and the underlying mechanisms. Diabetic models were established both in db/db mice and high glucose (HG)-induced human umbilical vein endothelial cells (HUVECs). Moreover, HUVECs were exposed to carbonyl cyanide m-chlorophenyl hydrazone (CCCP) to induce endothelial cell damage. Following Sal B treatment, pathological changes of thoracic aorta were investigated by hematoxylin and eosin, alcian blue (AB), elastic fiber, Masson, and reticular fiber staining. BCL2-associated X (BAX), B-cell lymphoma-2 (Bcl-2), Beclin1, Parkin and PTEN Induced Kinase 1 (Pink1) expression was detected by Western blot, immunohistochemistry, and immunofluorescence in thoracic aorta, HG- and CCCP-induced HUVECs. Cell scratch test, MitoTracker Red CMXRos staining and Flou-4 AM staining were separately presented to detect migration, mitochondrial activity and intracellular Ca2+ in HUVECs. Our results showed that Sal B significantly ameliorated hyperlipidemia, hyperglycemia, hyperinsulinemia, and insulin resistance in db/db mice. Furthermore, it significantly alleviated diabetes-induced vascular endothelial dysfunction according to histopathology analysis. In diabetic thoracic aorta, HG- and CCCP-induced HUVECs, Sal B distinctly increased Bcl-2 expression and reduced BAX, Beclin1, Parkin and Pink1 expression, thereby protecting endothelial cells from apoptosis and mitophagy. Moreover, Sal B markedly enhanced migration, mitochondrial activity and intracellular Ca2+ levels both in HG- and CCCP-induced HUVECs. Collectively, Sal B exhibited a potential to improve diabetes-induced endothelial and mitochondrial dysfunction through down-regulating apoptosis and mitophagy of endothelial cells. Abbreviations: DM: diabetes mellitus; T2DM: type 2 diabetes mellitus; Sal B: Salvianolic acid B; HG: high glucose; FBG: fasting blood glucose; TC: total cholesterol; TG: triglycerides; LDL-C: low-density lipoprotein cholesterol; HDL-C: high-density lipoprotein cholesterol; FINS: fasting insulin; HOMA-IR: homeostasis model assessment insulin resistance; QUICKI: quantitative insulin-sensitivity check index; H&E: hematoxylin and eosin; HUVECs: human umbilical vein endothelial cells; IHC: immunohistochemistry; CCCP: carbonyl cyanide m-chlorophenyl hydrazone; FCM: flow cytometry; CCK-8: cell counting kit-8

In combination with the advantages of gemini surfactants and sugar-based surfactants, a novel group of glucose derived gemini surfactants was synthesized and their surface properties were investigated. Some products could reduce the surface tension to 24.8–34.2 mN/m at the concentration level of 10−5 mol/L. Their aggregation behaviors were further studied by means of dynamic light scattering, X-ray diffraction and transmission electron microscopy. The results indicated that the investigated surfactants could self-assemble into vesicles in a wide range of concentrations. Since vesicles are widely used as nanocarrier in cosmetic and pharmaceutical field, the gemini surfactant 1c-based vesicles encapsulating resveratrol (RSV) as model compound were studied, and the results revealed that the vesicles displayed high loading capacity for RSV.