138 results on '"Liangzhi Xu"'
Search Results
2. Comparative efficacy and safety of metformin, anti-obesity agents, and myoinositol in improving IVF/ICSI outcomes and reducing ovarian hyperstimulation syndrome in women with polycystic ovary syndrome: a systematic review and network meta-analysis
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Lijun Lin, Ge Chen, Xiaoyong Qiao, Yan Chen, Hongxia Deng, and Liangzhi Xu
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Metformin ,Anti-obesity agents ,Inositol ,IVF ,ICSI ,OHSS ,Gynecology and obstetrics ,RG1-991 - Abstract
Abstract Purpose To compare the efficacy and safety of metformin, anti-obesity agents, and inositol with polycystic ovary syndrome (PCOS) undergoing in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI). Methods A comprehensive search was conducted in PubMed, Web of Science, Embase, Cochrane Library, and ClinicalTrials.gov for studies published in English up to October 26, 2024. Randomized controlled trials (RCTs) evaluating metformin, anti-obesity agents, and inositol were included. A network meta-analysis was performed using frequency statistical methods. Subgroup analyses were conducted based on controlled ovarian stimulation (COS) protocols and body mass index(BMI). The research protocol was registered with PROSPERO (registration code CRD42024502823). Results 20 RCTs were included with 1,827 patients assessed six different agents. Nineteen trials were rated low risk, with one rated moderate risk. Pairwise meta-analysis showed that metformin did not improve pregnancy outcomes but was associated with a reduced ovarian hyperstimulation syndrome (OHSS) risk (OR = 0.52, 95% CI 0.33–0.83), particularly in agonist protocols, along with lower E2 levels on the trigger day (SMD = -0.56, 95% CI -0.90 to -0.21) and increased side effects (OR = 6.85, 95% CI 4.32–10.86). Network meta-analysis confirmed no significant differences in pregnancy outcomes for these agents compared to controls, though both myoinositol and metformin reduced OHSS risk. Myoinositol was linked to a shorter gonadotropin duration (SMD = -1.21, 95% CI -2.03 to -0.38) and fewer side effects (OR = 0.23, 95% CI 0.06–0.83) compared to controls. Metformin led to lower E2 levels, a higher number of mature oocytes, and increased side effects (SMD = -376.52, 95% CI -610.83 to -142.22; SMD = 2.23, 95% CI 0.36–4.10; OR = 6.85, 95% CI 4.32–10.86) than controls. No studies reported an increased risk of fetal abnormalities. Conclusion Metformin and myoinositol may reduce OHSS risk in PCOS patients but did not significantly improve pregnancy outcomes. Metformin may lower OHSS risk in agonist protocol, reduce E2 levels on trigger day and increase mature oocytes but cause more side effects, while myoinositol may shorten gonadotropin duration with fewer side effects. Further robust RCTs are needed to confirm these findings.
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- 2024
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3. Systematic low-grade chronic inflammation and intrinsic mechanisms in polycystic ovary syndrome
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Hongxia Deng, Yan Chen, Jilong Xing, Nannan Zhang, and Liangzhi Xu
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chronic inflammation ,intrinsic mechanisms ,intestinal microecological ,steroid hormones ,polycystic ovary syndrome ,Immunologic diseases. Allergy ,RC581-607 - Abstract
Polycystic ovary syndrome (PCOS) is a prevalent endocrine and metabolic disorder affecting 6-20% of women of childbearing age worldwide. Immune cell imbalance and dysregulation of inflammatory factors can lead to systematic low-grade chronic inflammation (SLCI), which plays a pivotal role in the pathogenesis of PCOS. A significant higher infiltration of immune cells such as macrophages and lymphocytes and pro-inflammatory factors IL-6 and TNF-α has been detected in PCOS organ systems, impacting not only the female reproductive system but also other organs such as the cardiovascular, intestine, liver, thyroid, brain and other organs. Obesity, insulin resistance (IR), steroid hormones imbalance and intestinal microecological imbalance, deficiencies in vitamin D and selenium, as well as hyperhomocysteinemia (HHcy) can induce systematic imbalance between pro-inflammatory and anti-inflammatory cells and molecules. The pro-inflammatory cells and cytokines also interact with obesity, steroid hormones imbalance and IR, leading to increased metabolic imbalance and reproductive-endocrine dysfunction in PCOS patients. This review aims to summarize the dysregulation of immune response in PCOS organ system and the intrinsic mechanisms affecting SLCI in PCOS to provide new insights for the systemic inflammatory treatment of PCOS in the future.
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- 2024
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4. The association between circulating irisin levels and osteoporosis in women: a systematic review and meta-analysis of observational studies
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Xiaoyang Shen, Yan Chen, Jing Zhang, Meina Yang, Lu Huang, Jiaqi Luo, and Liangzhi Xu
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irisin ,osteoporosis ,bone mineral density ,fracture ,meta-analysis ,Diseases of the endocrine glands. Clinical endocrinology ,RC648-665 - Abstract
ObjectiveThis systematic review and meta-analysis aimed to investigate the association between circulating irisin levels and osteoporosis in women, exploring irisin’s potential role in the pathophysiology and management of osteoporosis.MethodWe searched PubMed, Embase, Web of Science, Cochrane Library, CNKI, WanFang, and VIP databases up to January 2023. The inclusion criteria were observational studies reporting on circulating irisin levels in women. The standardized mean difference (SMD) and correlation coefficients with a 95% confidence interval (CI) were used as the main effect measures under a random-effects model. Heterogeneity was evaluated using the Cochrane Q statistic and the I2 statistics. Subgroup analysis and univariate meta-regression analysis were performed to identify the sources of heterogeneity. The quality of the included study was assessed by the Newcastle-Ottawa Score. The quality of evidence was evaluated using the GRADE system. Publication bias was assessed using Begg’s and Egger’s test, and the trim-and-fill method. Sensitivity analysis was performed to assess the stability of the results.ResultsFifteen studies with a total of 2856 participants met the criteria. The analysis showed significantly lower irisin levels in postmenopausal osteoporotic women compared to non-osteoporotic controls (SMD = -1.66, 95% CI: -2.43 to -0.89, P < 0.0001; I2 = 98%, P < 0.00001) and in postmenopausal individuals with osteoporotic fractures than in non-fractures controls (SMD = -1.25, 95% CI: -2.15 to -0.34, P = 0.007; I2 = 97%, P < 0.00001). Correlation analysis revealed that irisin levels positively correlated with lumbar spine BMD (r = 0.37, 95% CI: 0.18 to 0.54), femoral BMD (r = 0.30, 95% CI: 0.18 to 0.42), and femoral neck BMD (r = 0.31, 95% CI: 0.14 to 0.47) in women. Despite significant heterogeneity, the robustness of the results was supported by using the random effects model and sensitivity analysis.ConclusionThe current evidence suggests that lower irisin levels are significantly associated with osteoporosis and fracture in postmenopausal women, suggesting its utility as a potential biomarker for early detection of osteoporosis and therapeutic target. However, further high-quality prospective research controlling for confounding factors is needed to clarify the relationship between irisin levels and osteoporotic outcomes.Systematic review registrationhttps://www.crd.york.ac.uk/PROSPERO, identifier CRD42023410264.
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- 2024
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5. Irisin alleviated the reproductive endocrinal disorders of PCOS mice accompanied by changes in gut microbiota and metabolomic characteristics
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Meina Yang, Hongxia Deng, Siyu Zhou, Danhua Lu, Xiaoyang Shen, Lu Huang, Yan Chen, and Liangzhi Xu
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irisin ,polycystic ovary syndrome ,folliculogenesis ,gut microbiota ,fecal metabolomics ,Microbiology ,QR1-502 - Abstract
IntroductionFolliculogenesis and oligo/anovulation are common pathophysiological characteristics in polycystic ovary syndrome (PCOS) patients, and it is also accompanied by gut microbiota dysbiosis. It is known that physical activity has beneficial effects on improving metabolism and promoting ovulation and menstrual cycle disorder in PCOS patients, and it can also modulate the gastrointestinal microbiota in human beings. However, the mechanism remains vague. Irisin, a novel myokine, plays a positive role in the mediating effects of physical activity.MethodsMice were randomly divided into the control group, PCOS group and PCOS+irisin group. PCOS model was induced by dehydroepiandrosterone (DHEA) and high-fat diet (HFD). The PCOS+irisin group was given irisin 400μg/kg intraperitoneal injection every other day for 21 days. The serum sex hormones were measured by radioimmunoassay. Hematoxylin and Eosin (H&E) Staining and immunohistochemistry (IHC) were conducted on ovarian tissue. The feces microbiota and metabolomic characteristics were collected by 16S rRNA gene sequencing and liquid chromatography-mass spectrometry (LC–MS).ResultsIn this study, we demonstrated that irisin supplementation alleviated reproductive endocrine disorders of PCOS mice, including estrous cycle disturbance, ovarian polycystic degeneration, and hyperandrogenemia. Irisin also improved the PCOS follicles dysplasia and ovulation disorders, while it had no significant effect on the quality of oocytes. Moreover, irisin could mitigate the decreased bacteria of Odoribacter and the increased bacteria of Eisenbergiella and Dubosiella in PCOS mice model. Moreover, irisin could alleviate the increased fecal metabolites: Methallenestril and PS (22:5(4Z,7Z,10Z,13Z,16Z)/ LTE4).ConclusionThese results suggest that irisin may alleviate the status of PCOS mice model by modulating androgen-induced gut microbiota dysbiosis and fecal metabolites. Hence, our study provided evidence that irisin may be considered as a promising strategy for the treatment of PCOS.
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- 2024
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6. Discovery of a normal-tension glaucoma-suspect rhesus macaque with craniocerebral injury: Hints of elevated translaminar cribrosa pressure difference
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Jian Wu, Qi Zhang, Xu Jia, Yingting Zhu, Zhidong Li, Shu Tu, Ling Zhao, Yifan Du, Wei Liu, Jiaoyan Ren, Liangzhi Xu, Hanxiang Yu, Fagao Luo, Wenru Su, Ningli Wang, Yehong Zhuo, and Yuanyuan Ji
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Medicine - Published
- 2024
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7. CDC25B Is a Prognostic Biomarker Associated With Immune Infiltration and Drug Sensitivity in Hepatocellular Carcinoma
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Zixiang Huang, Liangzhi Xu, Zhengqiang Wu, Xiaofeng Xiong, Linfei Luo, and Zhili Wen
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Genetics ,QH426-470 - Abstract
Cell division cycle 25B (CDC25B), a member of the CDC25 phosphatase family, plays a key role in cell cycle regulation. Studies have suggested its carcinogenic potential in various cancers, but the role of CDC25B in the development of hepatocellular carcinoma (HCC) remains poorly understood. The aim of this study was to clarify the role of CDC25B in HCC using bioinformatics and experiments. CDC25B expression data of HCC cancer tissues and paracancerous normal samples were obtained from The Cancer Gene Atlas (TCGA) and Gene Expression Omnibus (GEO) databases, and the relationship between CDC25B expression and the prognosis and degree of tumor differentiation of HCC patients was analyzed. CDC25B expression was verified in clinical HCC tissue samples using fluorescence quantitative polymerase chain reaction (q-PCR) and protein immunoblotting (Western blot). Gene set enrichment analysis (GSEA) was used to identify signaling pathways enriched in CDC25B expression, and differential genes (DEGs) were used to screen out coexpressed hub genes and construct protein-protein interaction (PPI) networks. 5-Ethynyl-2′-deoxyuridine (EDU) staining was used to compare the proliferation and differentiation ability of the HCC cell line (HCC-LM3) after knockdown of CDC25B. Finally, we investigated the mutation of CDC25B in HCC and the relationship between CDC25B expression and tumor cell infiltration of lymphocytes and some immune checkpoints as well as drug sensitivity. CDC25B was overexpressed in HCC tissues and correlated with poor prognosis and the degree of tumor differentiation in patients with HCC. The GSEA and PPI networks together revealed significantly upregulated signaling pathways, as well as functions, associated with the development of HCC when CDC25B was overexpressed. The EDU assay demonstrated that the ability of cells to differentiate value addedly was markedly reduced following the downregulation of CDC25B expression in HCC-LM3s. CDC25B was also involved in the formation of the tumor microenvironment (TME) and immune processes in HCC, and the high expression of CDC25B made patients less sensitive to some drugs. CDC25B can be used as a biomarker and immunotherapeutic target for poor prognosis and partial drug sensitivity in HCC, providing new ideas for HCC treatment.
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- 2024
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8. Design, methodology, and preliminary results of the non-human primates eye study
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Jian Wu, Wei Liu, Sirui Zhu, Hongyi Liu, Kezhe Chen, Yingting Zhu, Zhidong Li, Chenlong Yang, Lijie Pan, Ruyue Li, Caixia Lin, Jiaxin Tian, Jiaoyan Ren, Liangzhi Xu, Hanxiang Yu, Fagao Luo, Zhiwei Huang, Wenru Su, Ningli Wang, and Yehong Zhuo
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Non-human primates ,Eye study ,Methodology ,Ocular parameters ,Ophthalmology ,RE1-994 - Abstract
Abstract Purpose To describe the normative profile of ophthalmic parameters in a healthy cynomolgus monkey colony, and to identify the characteristic of the spontaneous ocular disease non-human primates (NHP) models. Methods The NHP eye study was a cross-sectional on-site ocular examination with about 1,000 macaques held in Guangdong Province, southeastern China. The NHPs (Macaca fascicularis, cynomolgus) in this study included middle-aged individuals with a high prevalence of the ocular disease. The NHP eye study (NHPES) performed the information including systematic data and ocular data. Ocular examination included measurement of intraocular pressure (IOP), anterior segment- optical coherence tomography (OCT), slit-lamp examination, fundus photography, autorefraction, electroretinography, etc. Ocular diseases included measurement of refractive error, anisometropia, cataract, pterygium, etc. Results A total of 1148 subjects were included and completed the ocular examination. The average age was 16.4 ± 4.93 years. Compared to the male participants, the females in the NHPES had shorter axial length and the mean Average retinal nerve fiber layer (RNFL) thickness (except for the nasal quadrants). The mean IOP, anterior chamber depth, lens thickness, axial length, central corneal thickness, choroid thickness and other parameters were similar in each group. Conclusion The NHPES is a unique and high-quality study, this is the first large macaque monkey cohort study focusing on ocular assessment along with comprehensive evaluation. Results from the NHPES will provide important information about the normal range of ophthalmic measurements in NHP.
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- 2023
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9. Spatially resolved gene regulatory and disease-related vulnerability map of the adult Macaque cortex
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Ying Lei, Mengnan Cheng, Zihao Li, Zhenkun Zhuang, Liang Wu, Yunong sun, Lei Han, Zhihao Huang, Yuzhou Wang, Zifei Wang, Liqin Xu, Yue Yuan, Shang Liu, Taotao Pan, Jiarui Xie, Chuanyu Liu, Giacomo Volpe, Carl Ward, Yiwei Lai, Jiangshan Xu, Mingyue Wang, Hao Yu, Haixi Sun, Qichao Yu, Chunqing Wang, Chi Wai Wong, Wei Liu, Liangzhi Xu, Jingkuan Wei, Dandan Chen, Zhouchun Shang, Guibo Li, Kun Ma, Le Cheng, Fei Ling, Tao Tan, Kai Chen, Bosiljka Tasic, Michael Dean, Weizhi Ji, Huanming Yang, Ying Gu, Miguel A. Esteban, Yuxiang Li, Ao Chen, Yuyu Niu, Hongkui Zeng, Yong Hou, Longqi Liu, Shiping Liu, and Xun Xu
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Science - Abstract
Cell type epigenetic and topographic information of primate brain is lacking. Here, authors identified transcriptional regulatory network, gradient expression pattern and disease vulnerability at cell type level in PFC, M1 and V1 of monkey brain by snRNAseq, snATAC-seq and Stereo-seq.
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- 2022
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10. Effects of vitamin D supplementation on ovulation and pregnancy in women with polycystic ovary syndrome: a systematic review and meta-analysis
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Meina Yang, Xiaoyang Shen, Danhua Lu, Jin Peng, Siyu Zhou, Liangzhi Xu, and Jing Zhang
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vitamin D ,polycystic ovary syndrome ,pregnancy ,ovulation ,meta-analysis ,Diseases of the endocrine glands. Clinical endocrinology ,RC648-665 - Abstract
ObjectiveTo evaluate the effect of vitamin D supplementation on pregnancy and ovulation in patients with polycystic ovary syndrome.MethodWe searched Pubmed, Medline (via Ovid, 1974 to 2020), EMBASE (via Ovid, 1974 to 2020), Cochrane Central Register of Controlled Trials (via Ovid), Web of Science, CNKI, WangFang and the Vip database from inception until April 2021. Two researchers independently screened articles, collected data and evaluated the quality, with Review manager 5.3 for meta-analysis.ResultsTotally 20 randomized controlled studies with 1961 subjects were included. Meta analysis showed that pregnancy rate [RR=1.44 (1.28, 1.62), p
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- 2023
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11. The influence of embryo stage on obstetric complications and perinatal outcomes following programmed compared to natural frozen-thawed embryo transfer cycles: a systematic review and meta-analysis
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Zhonghua Zhao, Yan Chen, Hongxia Deng, Lu Huang, Danhua Lu, Xiaoyang Shen, and Liangzhi Xu
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frozen–thawed embryo transfer ,programmed cycle ,natural cycle ,embryo ,embryo at time of transfer ,Diseases of the endocrine glands. Clinical endocrinology ,RC648-665 - Abstract
ObjectiveTo investigate the effect of embryo stage at the time of transfer on obstetric and perinatal outcomes in programmed frozen-thawed embryo transfer (FET) versus natural FET cycles.DesignSystematic review and meta-analysis.SettingNot applicable.Patient(s)Women with programmed frozen-thawed embryo transfer (FET) and natural FET.Intervention(s)The PubMed, MEDLINE, and EMBASE databases and the Cochrane Central Register of Controlled Trials (CCRT) were searched from 1983 to October 2022. Twenty-three observational studies were included.Primary outcome measureThe primary outcomes were hypertensive disorders of pregnancy (HDPs), gestational hypertension and preeclampsia (PE). The secondary outcomes were gestational diabetes mellitus (GDM), placenta previa, postpartum haemorrhage (PPH), placental abruption, preterm premature rupture of membranes (PPROM), large for gestational age (LGA), small for gestational age (SGA), macrosomia, and preterm delivery (PTD).Result(s)The risk of HDP (14 studies, odds ratio (OR) 2.17; 95% confidence interval (CI) 1.95-2.41; P
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- 2023
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12. Falsely elevated serum estradiol in woman of reproductive age led to unnecessary intervention and delayed fertility opportunity: a case report and literature review
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Jing Zhang, Liangzhi Xu, and Lin Qiao
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Estradiol ,Competitive chemiluminescent immunoassay ,Immunoassay interference ,Heterophile antibody ,Case report ,Gynecology and obstetrics ,RG1-991 ,Public aspects of medicine ,RA1-1270 - Abstract
Abstract Background The optimal management of patients in reproductive endocrinology relies on the accuracy and validity of sex hormone assays. Endogenous or exogenous substances can compete with the analyte. This competition can result in interfering errors and falsely indicate elevated serum levels. Obvious interference in estradiol assays appears to occur rarely. Consequently, clinicians who are not familiar with the potential of interference could be misled. In addition to unnecessary investigations and interventions and severe mental stress, falsely elevated estradiol results can result in missed or delayed fertility opportunities. Case A 28-year-old female with pregnancy demand was diagnosed with polycystic ovary syndrome, Hashimoto’s thyroiditis and subclinical hypothyroidism. She was found to have persistently elevated levels of serum estradiol in the early follicular phase (between 527 and 642 pg/mL). Screening workup was performed for nearly 11 months to find the causes. Serum tumor biomarkers were normal. Abdominal and pelvic computed tomography were negative for adrenal or adnexal masses. A left mesosalpinx cyst and benign pathological results were achieved by laparoscopic surgery. Hormonal substances and dietary supplements were absent, as determined by dietary records. Ultrasound confirmed follicles could grow slowly and eventually ovulate. Falsely elevated estradiol levels were suspected due to the discrepancy among high estradiol levels, follicle growth and normal gonadotropin levels. Immunological interference by heterophile antibody was finally verified by two competitive chemiluminescent immunoassay platforms (estradiol levels in the early follicle phase: 619 pg/mL, Siemens ADVIA CENTAUR and 60 pg/mL, Beckman, DxI 800). Successful clinical pregnancy was eventually achieved by combining induced ovulation, ultrasound monitoring and intercourse guidance. Conclusions Analytical interference and laboratory error should be suspicious at first when the clinical characteristics contradict the laboratory results of serum hormones. Measuring serum estradiol with another immunoassay platform is an easy and non-time-consuming method to exclude the heterophile interfering.
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- 2022
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13. Author Correction: Disordered metabolism in mice lacking irisin
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Yunyao Luo, Xiaoyong Qiao, Yaxian Ma, Hongxia Deng, Charles C. Xu, and Liangzhi Xu
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Medicine ,Science - Published
- 2023
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14. Elevated peripheral absolute monocyte count related to clinicopathological features and poor prognosis in solid tumors: Systematic review, meta‐analysis, and meta‐regression
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Shu Wen, Nan Chen, Ying Hu, Litao Huang, Jin Peng, Meina Yang, Xiaoyang Shen, Yang Song, and Liangzhi Xu
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inflammation ,monocyte ,prognosis ,solid tumor ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Abstract Background Absolute monocyte count (AMC) is often used to be assessed in cancer follow‐up, which has regained interest as a potential prognostic indicator in many solid tumors, though not consistently or comprehensively. In the present study, we set out to perform a comprehensive meta‐analysis of all available data regarding the prognostic significance of AMC in solid tumors. We also evaluated the association between AMC and clinical features in solid tumors. Methods A hazard ratio (HR) and corresponding 95% confidence interval (CI) or a p value (p) from eligible studies were extracted and subsequently pooled analyzed. Subgroup analyses and meta‐regression analyses were conducted according to the confounders of included studies. In addition, the relationships between AMC and clinical characteristics were also explored in the meta‐analysis. Results Overall, ninety‐three articles comprising 104 studies with 32229 patients were finally included. The results showed that elevated AMC was associated with worse overall survival (OS) (HR = 1.615; 95% CI: 1.475‐1.768; p
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- 2021
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15. Author Correction: Disordered metabolism in mice lacking irisin
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Yunyao Luo, Xiaoyong Qiao, Yaxian Ma, Hongxia Deng, Charles C. Xu, and Liangzhi Xu
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Medicine ,Science - Published
- 2021
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16. Irisin deletion induces a decrease in growth and fertility in mice
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Yunyao Luo, Xiaoyong Qiao, Yaxian Ma, Hongxia Deng, Charles C. Xu, and Liangzhi Xu
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Irisin ,Growth and development ,Hormone metabolism ,Reproduction ,Gynecology and obstetrics ,RG1-991 ,QH471-489 - Abstract
Abstract Background Irisin, which is cleaved from fibronectin type III domain-containing protein 5 (Fndc5), plays an important role in energy homeostasis. The link between energy metabolism and reproduction is well known. However, the biological actions of irisin in reproduction remain largely unexplored. Methods In this study, we generated Fndc5 gene mutation to create irisin deficient mice. Female wild-type (WT) and Fndc5 mutant mice were fed with standard chow for 48 weeks. Firstly, the survival rate, body weight and fertility were described in mice. Secondly, the levels of steroid hormones in serum were measured by ELISA, and the estrus cycle and the appearance of follicles were determined by vaginal smears and ovarian continuous sections. Thirdly, mRNA-sequencing analysis was used to compare gene expression between the ovaries of Fndc5 mutant mice and those of WT mice. Finally, the effects of exogenous irisin on steroid hormone production was investigated in KGN cells. Results The mice lacking irisin presented increased mortality, reduced body weight and poor fertility. Analysis of sex hormones showed decreased levels of estradiol, follicle-stimulating hormone and luteinizing hormone, and elevated progesterone levels in Fndc5 mutant mice. Irisin deficiency in mice was associated with irregular estrus, reduced ratio of antral follicles. The expressions of Akr1c18, Mamld1, and Cyp19a1, which are involved in the synthesis of steroid hormones, were reduced in the ovaries of mutant mice. Exogenous irisin could promote the expression of Akr1c18, Mamld1, and Cyp19a1 in KGN cells, stimulating estradiol production and inhibiting progesterone secretion. Conclusions Irisin deficiency was related to disordered endocrinology metabolism in mice. The irisin deficient mice showed poor growth and development, and decreased fertility. Irisin likely have effects on the expressions of Akr1c18, Mamld1 and Cyp19a1 in ovary, regulating the steroid hormone production. This study provides novel insights into the potential role of irisin in mammalian growth and reproduction.
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- 2021
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17. Dichorionic quadruplet pregnancy comprising monozygotic triplets and singleton after intracytoplasmic sperm injection and transfer of two fresh embryos: a case report
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Ying Nie, Xiaoyong Qiao, Sicong Li, Zhuo Pan, Jing Zhang, and Liangzhi Xu
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Medicine (General) ,R5-920 - Abstract
Monozygotic triplet pregnancies are very rare in assisted reproductive technology, and the relationship between monozygotic multiple pregnancies and several assisted reproductive techniques, including blastocyst transfer, remains unclear. Here, the case of a 28-year-old female patient with dichorionic quadruplet pregnancy following intracytoplasmic sperm injection and transfer of two day-3 fresh embryos, without assisted hatching, is reported. At 7 weeks following embryo transfer, the dichorionic quadruplet pregnancy, comprising monozygotic monochorionic triamniotic (MCTA) triplets plus a singleton, was detected by a transabdominal ultrasound scan. After counselling, the patient underwent selective reduction of the MCTA triplet pregnancy at 7 weeks after embryo transfer. The remaining singleton pregnancy was uneventful, resulting in a live birth at 38 + weeks. As the predictors of monozygotic multiple gestations remain poorly characterized, clinicians and patients should give great consideration to the risks associated with monozygotic multiple pregnancies, even if the patient has not undergone blastocyst transfer.
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- 2022
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18. Association of Estrogen Receptor Genes Polymorphisms With Polycystic Ovary Syndrome: A Systematic Review and Meta-Analysis Based on Observational Studies
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Siyu Zhou, Shu Wen, Yongcheng Sheng, Meina Yang, Xiaoyang Shen, Yan Chen, Deying Kang, and Liangzhi Xu
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polycystic ovary syndrome ,polymorphisms ,estrogen receptor ,meta-analysis ,gene variants ,Diseases of the endocrine glands. Clinical endocrinology ,RC648-665 - Abstract
PurposeControversial results existed in amounts of studies investigating the authentic association of estrogen receptor genes (ESR1 and ESR2) polymorphisms with the occurrence and progression of polycystic ovary syndrome (PCOS). The inconsistency might result from different loci, sample sizes, and ethnicities. To find the potential correlations between ESR1/ESR2 polymorphisms and PCOS risk, we conducted the first systematic review and meta-analysis to comprehensively summarize current studies in a large combined population.MethodsEligible studies were retrieved from PubMed, MEDLINE, EMBASE, Cochrane Library, CBM, CNKI, WANFANG, and VIP up to February 28, 2021. The quality of studies was assessed using the Newcastle–Ottawa Scale (NOS) scoring system. Odds ratios (ORs) and 95% confidence intervals (95%CIs) were calculated to synthesize data in five genetic models. Subgroup analyses were conducted by ethnicity. Heterogeneity and publication bias were also assessed. The protocol was registered in PROSPERO under the number CRD42021239200.ResultsA total of 8 studies involving 1,522 PCOS patients and 4,198 controls were included. No evidence demonstrated the association of ESR1 rs2234693 (OR=1.07 95%CI 0.98–1.18), ESR1 rs9340799 (OR=0.99 95%CI 0.69–1.43), or ESR2 rs4986938 (OR=1.06 95%CI 0.81–1.38) polymorphisms and PCOS risk in five genetic models. According to stratified subgroup analyses, ethnicity was considered the major source of heterogeneity. No publication bias was found in eligible studies.ConclusionThe present meta-analysis found no significant associations between the variants of ESR1 rs2234693, ESR1 rs9340799, ESR2 rs4936938, and individual PCOS susceptibility, even if ethnicity was taken into account.Systematic Review RegistrationThe protocol was registered in PROSPERO (available from https://www.crd.york.ac.uk/PROSPERO) with the ID number CRD42021239200.
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- 2021
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19. Association between social and family support and antenatal depression: a hospital-based study in Chengdu, China
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Ying Hu, Ying Wang, Shu Wen, Xiujing Guo, Liangzhi Xu, Baohong Chen, Pengfan Chen, Xiaoxia Xu, and Yuqiong Wang
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Antenatal depression ,Persistent depression ,Risk factors ,EPDS ,China ,Gynecology and obstetrics ,RG1-991 - Abstract
Abstract Background Antenatal depression (AD) is considered as one of the major health burdens and has adverse effects on the outcome of expectant mothers and newborns. The present study aims to investigate the prevalence of antenatal depression (AD), and to explore the potential risk factors of AD among pregnant women in Chengdu, including personal background, related social factors, family factors and cognitive factors. Methods The prospective nested case-control study included pregnant women who were in their second pregnancy and attended prenatal care at three tertiary hospitals and one regional hospital in Chengdu, China, between March 2015 and May 2016. Self-designed questionnaires were given to participants in their second and third trimesters to collect information on clinical and demographic characteristics, and a modified edition of Edinburgh Postnatal Depression Scale (EPDS) were used to measure AD. The logistic regression was applicated in analyses. Results A total of 996 pregnant women were included in analysis. Ninety-three women suffered from AD symptoms only in their second trimester, 96 only in their third trimester, and 107 displayed persistent depression in both trimesters. In the univariate analyses, age and marital relationships were linked with AD occurrence in both second and third trimester. In addition, increasing age, full-time job, higher education level, and no gender preference of spouse were associated with reduced persistent depression. Multivariate analysis showed that gender preference and marital relationship were the potential risk factors of persistent depression. Conclusions Age, marital relationship relationships, with parents-in-law, the negative recognition of this pregnancy and husband’s gender preference were found as risk factors of AD occurrence in some specific trimester. Gender preference of husbands and marital relationships were independently associated with persistent depression. These findings suggest that stronger family support can help improve mental health of pregnant women.
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- 2019
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20. ‘Standardized patients’ in teaching the communication skill of history-taking to four-year foreign medical undergraduates in the department of obstetrics and gynaecology
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Jing Zhang, Meng Cheng, Na Guo, Aiyun Xing, and Liangzhi Xu
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Standardized patient ,History-taking ,Communication skill ,Foreign students ,Medical undergraduate ,Obstetrics and gynaecology ,Special aspects of education ,LC8-6691 ,Medicine - Abstract
Abstract Background Many foreign students have difficulty taking histories from Chinese patients, especially in clinical context of the Department of Obstetrics and Gynaecology. The efficacy of using standardized patients to prepare foreign students for communicating with Chinese patients and taking their histories was evaluated in this study. Methods Ninety-four four-year foreign students were assigned to one of three clinical sub-departments (gynaecology, obstetrics, and reproductive endocrinology) to practice history-taking; after practicing in one sub-department, the students were then crossed over to a different department. The histories were taken from real patients in the sub-departments of obstetrics and reproductive endocrinology and from standardized patients in the sub-department of gynaecology. Prior to contact with real patients in the sub-department of reproductive endocrinology, the students practised with standardized patients. The quality levels of the case reports generated in the three departments were compared by repeated measures ANOVA. The attitudes, satisfaction and suggestions of the students were also investigated through a questionnaire. Results The local Chinese language spoken by the patients was thought to be the most common difficulty students (76.7%) encountered while taking patient histories. Two-thirds and one-third of the students were interested in taking histories from standardized and real patients, respectively. Most students (94.2%) thought that working with standardized patients was useful for practising communication skills with Chinese patients. The total scores of the case reports were significantly different among the three groups (P
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- 2019
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21. Temporal trends in the birth rates and perinatal mortality of twins: A population-based study in China.
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Changfei Deng, Li Dai, Ling Yi, Xiaohong Li, Kui Deng, Yi Mu, Ke Wang, Jing Tao, Qi Li, and Liangzhi Xu
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Medicine ,Science - Abstract
ObjectiveUntil now, little was known about the epidemiological characteristics of twins in China due to a lack of reliable national data. In this study, we aimed to analyze temporal trends and perinatal mortality of twins from China.MethodsData on twins between 2007 and 2014 were obtained from the China National Population-Based Birth Defects Surveillance System. Twin and singleton deliveries after at least 28 weeks of gestation were recruited and followed until postnatal day 42. Twinning rates were defined as the number of twin individuals per 1000 births(stillbirths and live births). The Weinberg's differential method was utilized to estimate the number of monozygotic and dizygotic twins.ResultsDuring 2007-2014, the twinning rate increased by 32.3% from 16.4 to 21.7 per 1000 total births with an average of 18.8‰. Among twins, both the perinatal mortality rate (26.1 per 1000 total births) and neonatal death rate (15.7 per 1000 live births) presented a downward tendency but remained at a high level. Large urban-rural and geographic disparities were identified in twinning rates, in perinatal and neonatal mortality, and in their temporal trends.ConclusionsThe upward trend of twinning rates in China paired with the relatively high rates of perinatal and neonatal mortality among twins highlights the need for improved perinatal care in the light of socio-demographic differences.
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- 2019
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22. High Intake of Energy and Fat in Southwest Chinese Women with PCOS: A Population-Based Case-Control Study.
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Jing Zhang, Ying Liu, Xiaofang Liu, Liangzhi Xu, Lingling Zhou, Liulin Tang, Jing Zhuang, Wenqi Guo, and Rong Hu
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Medicine ,Science - Abstract
Polycystic ovary syndrome (PCOS) is a common reproductive endocrinological disease with heterogeneous phenotype. Obesity contributes to the increased prevalence and severity of PCOS. Whether the intakes of major nutrients are higher in Chinese PCOS patients is still unknown.To study the intakes of total energy, protein, fat and carbohydrate in Southwest Chinese PCOS patients.1854 women were included in the cross-sectional study. A population-based case-control study was conducted. The dietary habits and nutrients intake status of 169 PCOS patients and 338 age-matched controls were investigated by the method of semi-quantitative food frequency questionnaire.The actual intake of total energy (P = 0.01) and fat (P = 0.01) were higher, but carbohydrate was lower (P = 0.01) in PCOS patients as compared with the controls. The energy percentage supplied by protein (12.33% ± 2.27% vs. 19.26% ± 5.91%, P
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- 2015
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23. Antidepresivos para el síndrome de ovario poliquístico
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Jing Zhuang, Xianding Wang, Liangzhi Xu, Taixiang Wu, and Deying Kang
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Medicine - Abstract
Antecedentes: La prevalencia de depresión en pacientes con síndrome de ovario poliquístico (SOPQ) es alta; un estudio ha revelado que supera en cuatro veces la de las pacientes sin SOPQ. Por lo tanto, es importante la evaluación sistemática de la efectividad y la seguridad de los antidepresivos para las pacientes con SOPQ. Objetivos: Evaluar la efectividad y la seguridad de los antidepresivos en cuanto al tratamiento de la depresión y otros síntomas en pacientes con SOPQ. Métodos de búsqueda: Se realizaron búsquedas en las siguientes bases de datos desde su inicio hasta junio de 2012: el registro de ensayos del Grupo Cochrane de Trastornos Menstruales y Subfertilidad (Cochrane Menstrual Disorders and Subfertility), el Registro Cochrane Central de Ensayos Controlados (Cochrane Central Register of Controlled Trials), MEDLINE, EMBASE, PsycINFO y Chinese National Knowledge Infrastructure, el metaRegister of Controlled Trials (controlled-trials.com), el National Institute of Health Clinical Trials register (clinicaltrials.gov) y en el portal de búsqueda de la World Health Organization International Trials Registry Platform (www.who.int/trialsearch/Default.aspx). Criterios de selección: Sólo se incluyeron en la revisión los ensayos controlados aleatorios (ECA) que estudiaban la efectividad y la seguridad de los antidepresivos en pacientes con SOPQ. Obtención y análisis de los datos: La calidad metodológica de los ensayos fue evaluada de forma independiente por dos revisores, de forma paralela a la extracción de datos. El riesgo de sesgo en los estudios incluidos se evaluó en seis dominios: 1. generación de secuencia: 2. ocultación de la asignación; 3. cegamiento de los participantes, personal y evaluadores de resultados; 4. completitud de los datos de resultado; 5. informe selectivo de los resultados; 6. otras fuentes potenciales de sesgo. Resultados principales: No se encontraron estudios que informaran ninguno de los resultados primarios de la revisión (puntuaciones de la depresión y del trastorno del estado de ánimo relacionado, calidad de vida y eventos adversos). Sólo un estudio con 16 mujeres era elegible para la inclusión. Este estudio comparó sibutramina versus fluoxetina en pacientes con SOPQ, e informó sólo resultados endócrinos y metabólicos. Estuvo poco claro si las participantes tenían problemas psicológicos al inicio. No se hallaron diferencias significativas entre los grupos para ninguno de los resultados medidos. Conclusiones de los autores: No existen pruebas sobre la efectividad y la seguridad de los antidepresivos para el tratamiento de la depresión y otros síntomas en pacientes con SOPQ.
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- 2013
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24. Chromosomal polymorphisms and susceptibility to menstrual disorders: a retrospective analysis of 24,578 women
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Ran, Cheng, Xiaoyan, Luo, Wenjie, Zhou, Xiaoyang, Zhou, Ying, Nie, Xiaoyong, Qiao, Zhilan, Yang, and Liangzhi, Xu
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- 2023
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25. Diagnostic Value of Ultrasound Imaging in Obstetrics and Gynecology Acute Abdomen.
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Sicong Li and Liangzhi Xu
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- 2021
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26. Laparoscopic Transcystic Common Bile Duct Exploration: 8-Year Experience at a Single Institution
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Jian Huang, Wei Hu, Jinghang Liu, Xinguo Tang, Yuting Fan, Liangzhi Xu, Tiande Liu, Hu Xiong, Wen Li, Xiaowei Fu, Bo Liang, and Lu Fang
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Gastroenterology ,Surgery - Published
- 2023
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27. The influence of perinatal maternal exposure to dibutyl phthalate on glucolipid metabolism in adult female offspring
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Kunyan, Zhou, Ran, Cheng, Mei, Zhu, Meina, Yang, Xiaoyang, Shen, Xiaoyan, Luo, Li, Ma, Liangzhi, Xu, and Jing, Zhang
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Nutrition and Dietetics ,Endocrinology, Diabetes and Metabolism ,Dibutyl Phthalate ,Rats ,Rats, Sprague-Dawley ,Phosphatidylinositol 3-Kinases ,Glucose ,Pregnancy ,Maternal Exposure ,Animals ,Insulin ,Female ,Obesity ,Proto-Oncogene Proteins c-akt - Abstract
Maternal exposure to dibutyl phthalate (DBP) may result in obesity in female offspring. However, the underlying mechanisms remain elusive.Sprague-Dawley rats were intraperitoneally injected with different doses of DBP and corn oil from gestational day 7 until the end of lactation. The weights, visceral fat percentage, serum lipid, insulin and glucose, protein levels of PI3K signal pathway in muscle were detected in F1 female offspring.Although the birth weight of F1 female offspring was not different among groups, the weights were heavier in DBP groups from postnatal day 7 to adult (P 0.001). The visceral adipose percentage in adult female offspring was increased by perinatal exposure to DBP (P 0.001). Decreased serum level of triglyceride (P = 0.001) in F1 female offspring was found in DBP group as compared to control, especially in medium and high DBP. However, none difference was found for fasting glucose, prolactin, HOMA-IR, fasting insulin, total cholesterol, adiponectin. Different protein levels of GPR30 were observed in muscle of female offspring among four groups (P = 0.016). The protein level of AKT seemed higher in DBP group but without statistical significance (P = 0.05). None difference was observed for the protein levels of PI3K, p-AKT, pAKT/AKT, PTEN, GLUT4, InsR, IRS.Maternal perinatal exposure to DBP might induce obesity and accumulation of visceral adipose tissue for the adult female offspring. Serum glucolipid and local signal transduction of PTEN/PI3K/AKT pathway in muscle were not adversely affected by perinatal exposure to DBP for adult female offspring.
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- 2022
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28. Systematic Review and Meta-analysis: Changes of Gut Microbiota before and after Menopause
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Meina Yang, Shu Wen, Jing Zhang, Jin Peng, Xiaoyang Shen, and Liangzhi Xu
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Postmenopause ,Estradiol ,Premenopause ,Biochemistry (medical) ,Clinical Biochemistry ,Genetics ,Humans ,Female ,General Medicine ,Menopause ,Molecular Biology ,Gastrointestinal Microbiome - Abstract
Objective. To systematically evaluate the differences in intestinal flora before and after menopause. To provide a possible mechanism for perimenopausal syndrome and provide a basis for probiotics as adjuvant therapy. Methods. MEDLINE, EMBASE, Web of Science, Cochrane Central Register of Controlled Trials (CENTRAL), CNKI, Wanfang, and VIP databases were searched. The included studies were case-control studies. Results. Three case-control studies were included, with a total of 156 people. At the phylum level, there were no differences between premenopausal and postmenopausal women. At the genus level, the relative abundances of A. odoratum and B. cholerae were higher in postmenopausal women than in premenopausal women, with no differences among other genera. The Shannon diversity index increased after menopause, but no differences were found. Only one study found a positive association of estradiol with Gammaproteobacteria and Myxococcales and a negative association with Prevotellaceae. Conclusions. On the basis of previous studies, it was found that there was no significant difference at the phylum level between postmenopausal women and premenopausal women, but Odoribacter and Bilophila increased at the genus level in postmenopausal women. The class of Gammaproteobacteria may be positively correlated with estradiol. Limited by the number of included studies, more high-quality clinical studies are needed for validation.
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- 2022
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29. Improving agricultural field parcel delineation with a dual branch spatiotemporal fusion network by integrating multimodal satellite data
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Cai, Zhiwen; Hu, Qiong; Zhang, Xinyu; Yang, Jingya; Wei, Haodong; Wang, Jiayue; Zeng, Yelu; Yin, Gaofei; Li, Wenjuan; You, Liangzhi; Xu, Baodong; Shi, Zhihua, http://orcid.org/0000-0001-7930-8814 You, Liangzhi, Cai, Zhiwen; Hu, Qiong; Zhang, Xinyu; Yang, Jingya; Wei, Haodong; Wang, Jiayue; Zeng, Yelu; Yin, Gaofei; Li, Wenjuan; You, Liangzhi; Xu, Baodong; Shi, Zhihua, and http://orcid.org/0000-0001-7930-8814 You, Liangzhi
- Abstract
PR, IFPRI3; ISI; DCA, Foresight and Policy Modeling (FPM); Transformation Strategies
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- 2023
30. Irisin: circulating levels in serum and its relation to gonadal axis
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Yunyao Luo, Xiaoyong Qiao, Liangzhi Xu, and Guoning Huang
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Mice ,Endocrinology ,Endocrinology, Diabetes and Metabolism ,Animals ,Obesity ,Insulin Resistance ,Hyperandrogenism ,Fibronectins ,Polycystic Ovary Syndrome - Abstract
Irisin is an exercise-induced myokine/adipokine in mice and humans that plays an important role in ‘browning’ of white adipose tissue and has shown great potential as a treatment for some metabolic diseases, such as obesity, insulin resistance, and inflammation. The circulating irisin level is reported to be associated with exercise, obesity, diet, diseases, and exposure to different pharmacological agents. Several studies have attempted to characterize the role of irisin in PCOS and other reproductive diseases, but contradictory results have been reported. Our previous study showed that irisin may serve further functions in folliculogenesis and fertility. In this review, we present the current knowledge on the physiology of irisin and its role in gonadal axis. Firstly, we describe irisin circulating levels and speculate on the potential mechanisms involved in irisin secretion and regulation. Then, we focus on the irisin levels in PCOS, and explore the relationships between, BMI, insulin resistance, and hyperandrogenism. Finally, we present the results from animal interventional studies and in vitro experiments to investigate the relationship between irisin and gonadal axis, indicating its novel effects on reproduction and fertility.
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- 2022
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31. Elevated Anti-Müllerian Hormone Levels in Newborns of Women with Polycystic Ovary Syndrome: a Systematic Review and Meta-analysis Based on Observational Studies
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Deying Kang, Liangzhi Xu, Siyu Zhou, Danhua Lu, Shu Wen, and Yongcheng Sheng
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Anti-Mullerian Hormone ,0301 basic medicine ,medicine.medical_specialty ,endocrine system diseases ,Offspring ,Metabolic health ,Anti-Müllerian hormone ,Cochrane Library ,Umbilical cord ,03 medical and health sciences ,0302 clinical medicine ,Child of Impaired Parents ,Humans ,Medicine ,Mass index ,Polycystic ovary syndrome ,030219 obstetrics & reproductive medicine ,biology ,business.industry ,Obstetrics ,Hyperandrogenism ,Infant, Newborn ,Obstetrics and Gynecology ,medicine.disease ,Polycystic ovary ,female genital diseases and pregnancy complications ,Observational Studies as Topic ,030104 developmental biology ,medicine.anatomical_structure ,Meta-analysis ,biology.protein ,Female ,business ,Reproductive Endocrinology: Original Article - Abstract
We performed this updated systematic review and meta-analysis to evaluate anti-Müllerian hormone levels (AMH) in newborns of mothers with polycystic ovary syndrome (PCOS) compared with healthy controls. A search of the literature was conducted in the PubMed, MEDLINE, EMBASE, Cochrane Library, CBM, CNKI, WANFANG, and VIP for articles to assess AMH levels in offspring of PCOS and non-PCOS mothers irrespective of language. These databases were searched from their inception to December 7, 2020. The quality of studies was assessed using the Newcastle-Ottawa Scale (NOS) scoring system. Standardized mean differences (SMDs) with 95% confidence intervals (CIs) were adopted to calculate the overall estimates with random-effects models. A total of 6 studies with 846 participants were included. The pooled analysis found an increased AMH level in the umbilical cord blood in newborns of PCOS mothers (SMD =0.62, 95% CI [0.28, 0.95]). Subgroup analyses revealed an elevation of AMH concentrations in female neonates, neonates born to American and Asian PCOS mothers. In addition, higher AMH levels were also found in studies diagnosed by the National Institute of Health (NIH) criteria, maternal clinical/biochemical hyperandrogenism, or maternal body mass index (BMI) >30 kg/m2. Meta-regression analysis suggested that diagnostic criterion contributed mostly to the high heterogeneity. We demonstrated that AMH levels in neonates born to PCOS mothers were essentially higher, which indicates that AMH may act as an enigmatic role in the pathogenesis of PCOS which inhibits folliculogenesis in the fetal stage.
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- 2021
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32. Author Correction: Disordered metabolism in mice lacking irisin
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Yaxian Ma, Hongxia Deng, Xiaoyong Qiao, Yunyao Luo, Charles C. Xu, and Liangzhi Xu
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Multidisciplinary ,business.industry ,Published Erratum ,Science ,MEDLINE ,Medicine ,Metabolism ,business ,Bioinformatics - Published
- 2021
33. Variation of Laparoscopic Ovarian Drilling for Clomiphene Citrate-resistant Patients with Polycystic Ovary Syndrome and Infertility: A Meta-analysis
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Xiaoyan Luo, Liangzhi Xu, Meina Yang, Xiaoyang Shen, Jing Zhang, and Kunyan Zhou
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Adult ,Infertility ,Ovarian drilling ,medicine.medical_specialty ,Adolescent ,Drug Resistance ,Clomiphene ,law.invention ,Miscarriage ,Young Adult ,03 medical and health sciences ,0302 clinical medicine ,Ovulation Induction ,Randomized controlled trial ,Pregnancy ,law ,medicine ,Humans ,030219 obstetrics & reproductive medicine ,Obstetrics ,business.industry ,Ovary ,Obstetrics and Gynecology ,Antral follicle ,medicine.disease ,Polycystic ovary ,Clinical trial ,Treatment Outcome ,030220 oncology & carcinogenesis ,Meta-analysis ,Female ,Laparoscopy ,Laser Therapy ,business ,Infertility, Female ,Polycystic Ovary Syndrome - Abstract
Objective To evaluate novel surgical variations of laparoscopic ovarian drilling (LOD) and compare with standard bilateral LOD. Data Sources Electronic databases were searched, including Cochrane database, CENTRAL, Ovid MEDLINE, Embase, PsycINFO, PubMed, Virtual Health Library, OpenSIGLE, ClinicalTrials.gov, ISRCTN, and The Chinese Clinical Trial Register in February 2019. Methods of Study Selection Randomized controlled trials (RCTs) evaluating LOD for patients with clomiphene citrate-resistant polycystic ovary syndrome and infertility and reporting reproductive outcomes, surgical complications, serum indexes, menses resumption, and ultrasound results were included. Quality and risk of bias were evaluated by 2 authors, respectively. Tabulation, Integration, and Results A total of 20 RCTs with 1615 patients were included. Evaluation of the quality of evidence for each study was based on each study's limitations of 5 outcome domains described by the Grading of Recommendations, Assessment, Development, and Evaluation and found to be moderate to very low. Live births were only reported by 4 studies. Unilateral LOD did not differ with bilateral LOD in reproductive outcomes, such as pregnancy (p = .11, I2 = 75%), ovulation (p = .08, I2 = 0%), miscarriage (p = .61), and menstruation resumption (p = .06). There was insufficient evidence regarding efficacy and safety of novel methods of LOD, such as transvaginal hydrolaparoscopy (1 RCT) and micro-LOD (3 RCTs). Evidence regarding the suitable number of ovarian punctures, duration of drilling, and antimullerian hormone or antral follicle numbers following LOD were inconclusive. Conclusion Unilateral LOD seems to be suitable replacement for conventional bilateral LOD for clomiphene citrate-resistant polycystic ovary syndrome, although more studies involving long-term reproductive efficacy, adverse events, and varying forms of LOD are warranted.
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- 2020
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34. Effects of leukocytospermia on the outcomes of assisted reproductive technology
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Xiaoyong Qiao, Rujun Zeng, Zhilan Yang, Liangzhi Xu, Qianhong Ma, Yezhou Yang, Yu Bai, Yihong Yang, and Peng Bai
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Male ,Endocrinology ,Pregnancy Rate ,Reproductive Techniques, Assisted ,Pregnancy ,Urology ,Humans ,Female ,Fertilization in Vitro ,Sperm Injections, Intracytoplasmic ,General Medicine ,Birth Rate ,Retrospective Studies - Abstract
Leukocytospermia is one of the common causes of male infertility, and its effects on the clinical outcomes of assisted reproduction are controversial. There are no recommendations for the management of leukocytospermia in cases of assisted reproductive technology (ART). To investigate the impact of leukocytospermia on ART, we retrospectively compared the clinical outcomes in ART couples with or without leukocytospermia and further analysed the impact of the insemination method itself by split insemination treatment in ART couples with leukocytospermia. In this study, leukocytospermia was detected in 133 patients, namely 63 in the conventional in vitro fertilization (IVF) group, 38 in the intracytoplasmic sperm injection (ICSI) group and 32 in the split insemination group. Leukocytospermia has a negative influence on the parameters of semen samples; however, leukocytospermia did not affect the clinical outcomes of IVF or ICSI. Different insemination methods did not affect the fertilization, clinical pregnancy or live birth rates. In the split insemination study, no significant differences in clinical pregnancy and live birth rates between the IVF and ICSI groups were found; however, the numbers of two pronuclei (2PN), available embryos and good-quality embryos in the ICSI group were higher than those in the IVF group. Leukocytospermia may be a risk factor affecting semen parameters, and more attention should be given to IVF insemination. Leukocytospermia has no significant negative effect on the outcomes of ART. ICSI may obtain better embryos than IVF, but it cannot improve the clinical pregnancy and live birth rates.
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- 2022
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35. Different regimens of menopausal hormone therapy for improving sleep quality: a systematic review and meta-analysis
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Zhuo Pan, Shu Wen, Xiaoyong Qiao, Meina Yang, Xiaoyang Shen, and Liangzhi Xu
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Sleep Quality ,Hormone Replacement Therapy ,Applied Mathematics ,General Mathematics ,Obstetrics and Gynecology ,Humans ,Estrogens ,Female ,Menopause ,Progesterone - Abstract
Long-term sleep disturbances in menopausal women are closely related to cardiovascular disorders, metabolic disorders, and cognitive impairment. At present, hormone therapy (HT) is a standard treatment for menopausal symptoms. However, it remains unclear whether HT can improve sleep quality.We did a systematic review and meta-analysis to assess the effects of different HT regimens on menopausal sleep quality.We systematically searched MEDLINE, Embase, the Cochrane Central Register of Controlled Trials, PsycINFO, CINAHL, and Web of Science for randomized controlled trials of menopausal HT on sleep disturbances up to June 14,2021. Information about ongoing and unpublished trials was collected by searching WHOICTRP and ClinicalTrials.gov. Our primary outcome was sleep quality with objective measurements. We estimated the standardized mean difference (SMD) using random-effects models.We identified a total of 3,059 studies and finally included 15 studies in the meta-analysis. Compared with placebo, HT improved self-reported sleep outcomes (SMD = -0.13; 95% CI, -0.18 to -0.08, P 0.00001 and I2 = 41%), but not sleep parameters measured by polysomnography. Subgroup analyses according to the regimen of HT showed that 17β-estradiol (17β-E2) (SMD = -0.34; 95% CI, -0.51 to -0.17, P 0.0001, and I2 = 0%) and conjugated equine estrogens (SMD = -0.10; 95% CI, -0.12 to -0.07, P 0.00001, and I2 = 0%) improved sleep quality. Moreover, transdermal administration (SMD = -0.35; 95% CI, -0.64 to -0.06, and P = 0.02) was more beneficial than oral (SMD = -0.10; 95% CI, -0.14 to -0.07, and P 0.00001). In addition, the combination of estrogen and progesterone had a positive effect on sleep disturbance (SMD = -0.10; 95% CI, -0.13 to -0.07, P 0.00001, and I2 = 0%), while estrogen monotherapy did not. The results showed that estrogen/micronized progesterone (SMD = -0.22; 95% CI, -0.37 to -0.06, P = 0.007, and I2 = 0%) and estrogen/medroxyprogesterone acetate (SMD = -0.10; 95% CI, -0.13 to -0.07, P 0.00001, and I2 = 0%) could alleviate sleep disturbance.HT has a beneficial effect on sleep disturbance to some extent, and the formulations and routes of administration of hormonal agents influence the effect size.
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- 2022
36. Ultrasound‐guided transvaginal ovarian needle drilling for clomiphene‐resistant polycystic ovarian syndrome in subfertile women
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Xin Pan, Taixiang Wu, Jing Zhang, Linglingli Kong, Liangzhi Xu, Guan J Liu, and Liulin Tang
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Infertility ,Ovarian drilling ,medicine.medical_specialty ,Pregnancy Rate ,media_common.quotation_subject ,Ovarian hyperstimulation syndrome ,Clomiphene ,Miscarriage ,Ovulation Induction ,Pregnancy ,Humans ,Medicine ,Pharmacology (medical) ,Ovulation ,Ultrasonography, Interventional ,media_common ,Obstetrics ,business.industry ,Fertility Agents, Female ,medicine.disease ,Pregnancy rate ,Female ,Live birth ,business ,Infertility, Female ,Polycystic Ovary Syndrome - Abstract
BACKGROUND: Ovulatory disturbance is a key diagnostic feature of polycystic ovarian syndrome (PCOS), leading to infertility and correspondingly heavy disease burden. Many therapeutic strategies have been used to induce ovulation for women with PCOS who are infertile. Ultrasound‐guided transvaginal ovarian needle drilling (UTND) is a novel surgical method used to induce ovulation for women with clomiphene‐resistant PCOS at the outpatients clinic. Nevertheless, the quality in most of the studies seemed low, and the safety and efficacy of UTND is still uncertain. OBJECTIVES: To evaluate the efficacy and safety of UTND for subfertile women with clomiphene‐resistant PCOS. SEARCH METHODS: We searched the Cochrane Gynaecology and Fertility Group (CGFG) Specialised Register, CENTRAL, MEDLINE, Embase, and six other databases to November 2018. We checked conference abstracts from the 2018 ESHRE, reference lists, and clinical trials registries. We contacted experts and specialists in the field. SELECTION CRITERIA: We included randomised controlled trials (RCTs) comparing UTND to laparoscopic ovarian drilling (LOD), and UTND combined with gonadotropins to gonadotropins alone for women of reproductive age with clomiphene‐resistant PCOS and infertility. DATA COLLECTION AND ANALYSIS: Two review authors independently screened appropriate trials for inclusion, assessed methodological quality and risk of bias, and extracted data. The primary outcomes were live birth rate and incidence of surgical complications (bleeding and infection). We included ovarian hyperstimulation syndrome (OHSS) as a secondary outcome. Meta‐analyses could only be conducted for the secondary outcomes pregnancy rate and ovulation rate in the comparison of UTND versus LOD using a random‐effect model. We calculated odds ratios (OR) with 95% confidence intervals (CI) for dichotomous data. We assessed the overall quality of the evidence by applying GRADE criteria. MAIN RESULTS: We included five trials involving 639 clomiphene‐resistant women with PCOS. Three studies compared UTND with LOD, and two compared UTND combined with gonadotropins with gonadotropins alone. The evidence was of low to very low quality. The main limitations were serious risk of bias due to poor reporting of methods, inconsistency resulting from heterogeneity, imprecision induced by limited sample size, and lack of reporting of clinically relevant outcomes such as live birth and surgical complications. UTND versus LOD No studies reported on the main outcome live birth. One study reported on surgical complications; however, the evidence for this outcome was of very low quality because it was based on one study with small sample size and there were no events in either arm. Thus, we are uncertain whether there is any difference in surgical complications between UTND and LOD. We are also uncertain whether there is any difference in pregnancy rate when comparing UTND with LOD (OR 0.54, 95% CI 0.28 to 1.03; I(2) = 56%; 3 RCTs, n = 473; very‐low quality evidence). UTND may lead to a slight decrease in ovulation rate when compared to LOD (OR 0.66, 95% CI 0.45 to 0.97; I(2) = 0%; 3 RCTs, n = 473; low‐quality evidence). This suggests that among clomiphene‐resistant women with PCOS using LOD with an expected ovulation rate of 69.5%, the ovulation rate among women using UTND may be between 50.6% and 68.8% No studies reported on the outcomes OHSS and multiple pregnancy. There was also insufficient evidence to reach a conclusion regarding miscarriage as there was only one study of very low quality. UTND combined with gonadotropins versus gonadotropins alone No studies reported on the main outcomes live birth and incidence of surgical complications. The evidence for the outcomes OHSS, pregnancy, ovulation, miscarriage, and multiple pregnancy in this comparison was of very low quality. Thus, we are uncertain whether there is any difference in these outcomes for women with clomiphene‐resistant PCOS using UTND combined with gonadotropins as compared with gonadotropins. AUTHORS' CONCLUSIONS: Based on very low‐quality evidence, It is uncertain whether there is any difference in pregnancy rate, incidence of surgical complications, and miscarriage rate between UTND and LOD in women with clomiphene‐resistant PCOS. UTND may lead to a slight decrease in ovulation rate when compared to LOD. No studies reported on the outcomes live birth rate, incidence of OHSS, and multiple pregnancy rate. No studies reported on the main outcomes live birth and surgical complications for the comparison UTND combined with gonadotrophins versus gonadotrophins alone. The evidence for the outcomes OHSS, pregnancy, ovulation, miscarriage, and multiple pregnancy in this comparison was of very low quality. Thus, it is unclear if there is a difference in any of the outcomes between UTND combined with gonadotrophins versus gonadotrophins alone.
- Published
- 2021
37. Association of gene polymorphisms in the anti-Müllerian hormone signalling pathway with ovarian function: a systematic review and meta-analysis
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Ran Cheng, Ying Nie, Chen Hui, Liangzhi Xu, Wei Xiong, Yaxian Ma, Xiaoyong Qiao, Xiaoyan Luo, and Ying Hu
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Anti-Mullerian Hormone ,Oncology ,endocrine system ,medicine.medical_specialty ,Receptors, Peptide ,endocrine system diseases ,Subgroup analysis ,Single-nucleotide polymorphism ,Primary Ovarian Insufficiency ,Polymorphism, Single Nucleotide ,Ovulation Induction ,Polymorphism (computer science) ,Internal medicine ,Genotype ,medicine ,Humans ,Ovarian Reserve ,Ovarian reserve ,biology ,business.industry ,Obstetrics and Gynecology ,Anti-Müllerian hormone ,Polycystic ovary ,female genital diseases and pregnancy complications ,Reproductive Medicine ,Meta-analysis ,biology.protein ,Female ,Menopause ,business ,Receptors, Transforming Growth Factor beta ,Signal Transduction ,Developmental Biology - Abstract
This systematic review evaluated whether single nucleotide polymorphisms of AMH and AMHRII genes are associated with ovarian function. A literature search of PubMed and Embase was complemented by hand searches in the reference lists. Eight studies involving 3155 participants were included in a meta-analysis and 10 studies included for description. For AMH c.146T>G polymorphism, no significant difference in serum anti-Mullerian hormone (AMH) levels was found between participants with different genotypes (weighted mean difference [WMD] 0.42, 95% confidence interval [CI] −0.16 to 0.99). Subgroup analyses showed similar results for the European region and in healthy and infertile populations. Regarding AMHRII −482 A>G, there was no significant difference in serum AMH levels between participants with A/A genotype and G/A or G/G genotype carriers (WMD –0.04, 95% CI −0.31 to 0.23). In subgroup analysis, an interesting trend was confirmed in healthy women and polycystic ovary syndrome (PCOS) patients (WMD –0.36, 95% CI −0.63 to –0.09, P = 0.009; WMD 0.46, 95% CI 0.15 to 0.77, P = 0.004). The review suggests that AMH c.146T>G is not associated with AMH levels, while AMHRII −482 A>G may be related to AMH levels in PCOS and healthy subgroups. However, the impact of polymorphisms in the AMH signalling pathway on ovarian function still requires further investigation.
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- 2019
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38. Role of PCSK9 in lipid metabolic disorders and ovarian dysfunction in polycystic ovary syndrome
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Jin-Hu Zhang, Li Nie, Liangzhi Xu, Hanna Li, Li Zhang, Yi Lei, Li-Min Yue, Xue-Qin Zhang, Mei-Jiao Wang, Dan Zhao, Dong-zhi Yuan, Wen-Jing Guo, Min Liu, Yichen Wang, and Yun Long
- Subjects
0301 basic medicine ,medicine.medical_specialty ,endocrine system diseases ,Endocrinology, Diabetes and Metabolism ,Population ,Ovarian Disorder ,Dehydroepiandrosterone ,030209 endocrinology & metabolism ,Ovary ,Mice ,03 medical and health sciences ,0302 clinical medicine ,Endocrinology ,Internal medicine ,Animals ,Humans ,Medicine ,Ovarian Diseases ,education ,education.field_of_study ,business.industry ,PCSK9 ,nutritional and metabolic diseases ,Lipid metabolism ,Lipid Metabolism ,Polycystic ovary ,female genital diseases and pregnancy complications ,Disease Models, Animal ,030104 developmental biology ,medicine.anatomical_structure ,Liver ,Case-Control Studies ,Female ,Proprotein Convertase 9 ,business ,Polycystic Ovary Syndrome ,Lipoprotein - Abstract
Proprotein convertase subtilisin/kexin type 9 (PCSK9) plays a critical role in the cholesterol metabolism by negatively regulating the low-density lipoprotein receptor (LDLR). Lipid metabolic and ovarian disorders are the common clinical manifestation of polycystic ovary syndrome (PCOS). Here, we intended to elucidate the role of PCSK9 in the pathogenesis of PCOS conducted on a human population in case-control design and animal part in an interventional study.We firstly investigated the serum levels of PCSK9 in 46 PCOS patients compared with 49 healthy women as controls, and then developed a PCOS mouse model induced by dehydroepiandrosterone (DHEA) and a high-fat diet (HFD) to determine the role of PCSK9 in abnormal lipid metabolism and ovarian dysfunction of PCOS in four groups (n = 40 per group): control, PCOS mice, PCOS plus alirocumab group, and PCOS plus vehicle group. The expression of PCSK9 in their serum, hepatic and ovarian tissues, serum lipid profiles and hormones were measured. Additionally, mRNA and protein expression levels of LDLR in hepatic and ovarian tissues, ovarian morphology and function were determined. Finally, we used freshly isolated theca-interstitial cells (TICs) and granulosa cells (GCs) from prepubertal normal mice to explore the effect of PCSK9 on LDL uptake of the cells.Serum PCSK9 concentrations were higher in PCOS patients than normal controls (P 0.05). The PCOS model mice exhibited significantly increased serum levels of total cholesterol (TC), LDL-C and high-density lipoprotein-cholesterol (HDL-C; P 0.001, P 0.001, P = 0.0004, respectively). Moreover, the serum PCSK9 protein level was significantly increased in PCOS mice (P = 0.0002), which positively correlated with serum LDL-C (r = 0.5279, P = 0.0004) and TC (r = 0.4151, P = 0.035). In both liver and ovary of PCOS mice, PCSK9 mRNA and protein levels were significantly increased (P 0.05), but LDLR levels were significantly decreased (P 0.05). Furthermore, alirocumab inhibiting PCSK9 partly increased in LDLR expression in both liver and ovary in PCOS mice, also ameliorated the lipid metabolic disorders and pathological changes of ovarian morphology and function and serum reproductive hormones but not in the PCOS plus vehicle group. In vitro experiment, recombinant PCSK9 decreased LDL uptake in TICs and GCs (P 0.001, P = 0.0011, respectively), which were partly reversed by alirocumab (P 0.001, P = 0.012, respectively).Abnormal high expression of PCSK9 in the blood, liver and ovary may be involved in the pathogenesis of PCOS by affecting lipid metabolism and ovarian function, and the inhibition of PCSK9 may partly reverse the pathological changes of PCOS. Our research suggests a possibility of PCSK9 as a new attractive target for diagnosis and treatment of PCOS.
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- 2019
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39. Perinatal maternal chronic exposure to dibutyl phthalate promotes visceral obesity in adult female offspring
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Jing Zhang, Meina Yang, Xiaoyan Luo, Ran Cheng, Li Ma, Liangzhi Xu, Xiaoyang Shen, and Kunyan Zhou
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medicine.medical_specialty ,Triglyceride ,Adiponectin ,Perinatal Exposure ,Dibutyl phthalate ,Offspring ,business.industry ,Insulin ,medicine.medical_treatment ,Adipose tissue ,chemistry.chemical_compound ,medicine.anatomical_structure ,Endocrinology ,chemistry ,Internal medicine ,Lactation ,medicine ,business - Abstract
IntroductionMaternal exposure to dibutyl phthalate (DBP) may result in glucolipid dysfunction in female offspring. However, the underlying mechanisms remain elusive. We hypothesized that chronic maternal DBP exposure could induce abnormal metabolism of glucolipid.Materials and methodsSprague-Dawley rats were intraperitoneally injected with different doses of DBP, estradiol, and corn oil from gestational day 7 until the end of lactation. The weights, visceral fat percentage, serum lipid, insulin and glucose, protein levels of PI3K signal pathway in muscle were detected in F1 female offspring.ResultsAlthough the birth weight of F1 female offspring was not different among groups, the weights were heavier in DBP groups from postnatal day 7 to adult (PConclusionMaternal perinatal exposure to DBP induced obesity and accumulation of visceral adipose tissue for the adult female offspring. Serum glucolipid and local signal transduction of PTEN/PI3K/AKT pathway in muscle were not adversely affected by perinatal exposure to DBP for adult female offspring.
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- 2021
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40. Reproductive Health in First-degree Relatives of Patients With Polycystic Ovary Syndrome: A Review and Meta-analysis
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Liangzhi Xu, Yitong Cai, Dan Shan, Li Zou, Yongchun Shen, and Jinbiao Han
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Anti-Mullerian Hormone ,medicine.medical_specialty ,Endocrinology, Diabetes and Metabolism ,Clinical Biochemistry ,Population ,Biochemistry ,Severity of Illness Index ,chemistry.chemical_compound ,Endocrinology ,Dehydroepiandrosterone sulfate ,Internal medicine ,medicine ,Prevalence ,Humans ,Family ,Testosterone ,First-degree relatives ,education ,education.field_of_study ,business.industry ,Free androgen index ,Biochemistry (medical) ,Luteinizing Hormone ,Polycystic ovary ,Systematic review ,Reproductive Health ,chemistry ,Meta-analysis ,Cohort ,Female ,Insulin Resistance ,business ,Polycystic Ovary Syndrome - Abstract
Context Polycystic ovary syndrome (PCOS) is a highly heritable disease. Emerging evidence elucidated the elevated prevalence of reproductive abnormalities in first-degree relatives (FDRs) of patients with PCOS. Objective To explore the reproductive health in FDRs of patients with PCOS. Methods Ten databases were searched in December 2020 (PubMed, Embase, Web of Science, Cochrane Central Register of Controlled Trials, ClinicalTrials.gov, Chinese Biological Medical Literature, Chinese National Knowledge Infrastructure, Chinese Journals Full-text Database, WanFang, and World Health Organization international clinical trials registry platform). This study included cohort, case-control, or cross-sectional studies. The Preferred Reporting Items for Systematic Reviews and Meta-Analysis statement was followed. Dichotomous data from each of the eligible studies were combined by the Mantel-Haenszel model. Standard mean differences with 95% CIs were assessed. Heterogeneities were assessed using I2 statistics, and the quality of evidence was evaluated by a US Agency for Healthcare Research and Quality Evidence-based Practice Center program and Grading of Recommendations, Assessment, Development and Evaluation approach. Results Thirty-eight studies were included. The prevalence of PCOS (0.22; 95% CI, 0.16 to 0.29), menstrual irregularities (0.28; 95% CI, 0.22 to 0.34, P Conclusion The findings of this analysis suggested that FDRs of patients with PCOS suffered from reproductive endocrinological dysregulations. Thus, more attention should be focused on this population.
- Published
- 2021
41. Maternal Perinatal Exposure to Dibutyl Phthalate Promotes Ovarian Dysfunction in Adult Female Offspring via Downregulation of TGF-β2 and TGF-β3
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Jing Zhang, Kunyan Zhou, Ran Cheng, Meina Yang, Xiaoyang Shen, Xiaoyan Luo, and Liangzhi Xu
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Estradiol ,Ovary ,Obstetrics and Gynecology ,Down-Regulation ,Dibutyl Phthalate ,Rats ,Rats, Sprague-Dawley ,Transforming Growth Factor beta2 ,Transforming Growth Factor beta3 ,Receptors, Estrogen ,Maternal Exposure ,Pregnancy ,Prenatal Exposure Delayed Effects ,Animals ,Female ,Estrogen Receptor Antagonists ,RNA, Messenger ,Progesterone - Abstract
Maternal exposure to dibutyl phthalate (DBP) may result in ovarian dysfunction in female offspring. However, the underlying mechanisms remain elusive. Pregnant Sprague-Dawley rats were intraperitoneally injected with different doses of DBP, estradiol, and corn oil from gestational day 7 until the end of lactation. The reproductive characteristics, mRNA, and protein expression of ovaries for the adult female offspring were compared. KGN cells were cultured in vitro with DBP, estrogen receptor antagonist, or ALK-5 inhibitor. Genes, proteins, estradiol, and progesterone expressed by KGN, cell proliferation, and apoptosis were measured respectively. Maternal perinatal exposure to DBP induced prolonged estrous period, increased secondary follicles, significant decreased mRNA, and protein levels of TGF-β2, TGF-β3, and TGF-βRII in ovaries of the adult female offspring, but none difference for serum levels of sex hormones, ovarian TGF-β1, and estrogen receptor. The mRNA levels of LHR, FSHR, and CYP19a in ovaries were also decreased. DBP might decrease the mRNA of TGF-β2, TGF-β3, and TGF-βR II of KGN. DBP can inhibit the mRNA of CYP19 at 24 h, which might be blocked by the estrogen receptor antagonist, whose effects were attenuated at 48 h. DBP combined with FSH might time-dependently regulate the gene expression of TGF-βR II, inhibitory at 24 h, but stimulative at 48 h, which could be blocked by the ALK5 inhibitor. However, the protein expressed by KGN was not influenced by DBP. DBP stimulated the proliferation of KGN at 24 h, which could be blocked by estrogen receptor antagonist, but attenuated at 48 h. The progesterone in culture medium secreted by KGN was decreased by DBP at 24 h. Maternal perinatal exposure to DBP induced decreased gene expression of TGF-β signaling and functional proteins in ovaries of the adult female offspring. Molecular cross-talk between estrogen receptor and TGF-β signaling pathway may play role in the mechanism of granulosa dysfunction induced by DBP.
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- 2021
42. Cytoplasmic domain and enzymatic activity of ACE2 is not required for PI4KB dependent endocytosis entry of SARS-CoV-2 into host cells
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Hong Wang, Yang H, Xin Zhao, Xun M, Liangzhi Xu, and Bing Liu
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chemistry.chemical_classification ,Enzyme ,biology ,chemistry ,Drug development ,Viral entry ,Cytoplasm ,Lentivirus ,Angiotensin-converting enzyme 2 ,biology.organism_classification ,Endocytosis ,PI4KB ,Cell biology - Abstract
The recent COVID-19 pandemic poses a global health emergency. Cellular entry of the causative agent SARS-CoV-2 is mediated by its spike protein interacting with cellular receptor- human angiotensin converting enzyme 2 (ACE2). Here, we used lentivirus based pseudotypes bearing spike protein to demonstrate that entry of SARS-CoV-2 into host cells is dependent on clathrin-mediated endocytosis, and phosphoinositides play essential role during this process. In addition, we showed that the intracellular domain and the catalytic activity of ACE2 is not required for efficient virus entry. These results provide new insights into SARS-CoV-2 cellular entry and present potential targets for drug development.
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- 2021
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43. NEWBORNS OF WOMEN WITH POLYCYSTIC OVARY SYNDROME (PCOS) HAVE HIGHER ANTI-MULLERIAN HORMONE (AMH) LEVELS: A SYSTEMATIC REVIEW AND META-ANALYSIS
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Deying Kang, Liangzhi Xu, Siyu Zhou, Shu Wen, and Yongcheng Sheng
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medicine.medical_specialty ,biology ,business.industry ,Polycystic ovary syndrome (PCOS) ,Obstetrics and Gynecology ,Anti-Müllerian hormone ,medicine.disease ,Endocrinology ,Reproductive Medicine ,Internal medicine ,Meta-analysis ,biology.protein ,Medicine ,business - Published
- 2021
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44. Spatially resolved gene regulatory and disease vulnerability map of the adult Macaque cortex
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Ying Lei, Mengnan Cheng, Zihao Li, Zhenkun Zhuang, Liang Wu, Yunong Sun, Lei Han, Zhihao Huang, Yuzhou Wang, Zifei Wang, Liqin Xu, Yue Yuan, Shang Liu, Taotao Pan, Jiarui Xie, Chuanyu Liu, Giacomo Volpe, Carl Ward, Yiwei Lai, Jiangshan Xu, Mingyue Wang, Hao Yu, Haixi Sun, Qichao Yu, Chunqing Wang, Chi Wai Wong, Wei Liu, Liangzhi Xu, Jingkuan Wei, Dandan Chen, Zhouchun Shang, Guibo Li, Kun Ma, Le Cheng, Fei Ling, Tao Tan, Kai Chen, Bosiljka Tasic, Michael Dean, Weizhi Ji, Huanming Yang, Ying Gu, Miguel A. Esteban, Yuxiang Li, Ao Chen, Yuyu Niu, Hongkui Zeng, Yong Hou, Longqi Liu, Shiping Liu, and Xun Xu
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Cell type ,medicine.anatomical_structure ,Visual cortex ,biology ,biology.animal ,Cortex (anatomy) ,medicine ,Computational biology ,Prefrontal cortex ,Transcription factor ,Macaque ,Gene ,Chromatin - Abstract
Single cell approaches have increased our knowledge about the cell type composition of the non-human primate (NHP), but a detailed characterization of area-specific regulatory features remains outstanding. We generated single-cell chromatin accessibility (single-cell ATAC) and transcriptomic data of 358,237 cells from prefrontal cortex (PFC), primary motor cortex (M1) and primary visual cortex (V1) of adult cynomolgus monkey brain, and integrated this dataset with Stereo-seq (Spatio-Temporal Enhanced REsolution Omics-sequencing) of the corresponding cortical areas to assign topographic information to molecular states. We identified area-specific chromatin accessible sites and their targeted genes, including the cell type-specific transcriptional regulatory network associated with excitatory neurons heterogeneity. We reveal calcium ion transport and axon guidance genes related to specialized functions of PFC and M1, identified the similarities and differences between adult macaque and human oligodendrocyte trajectories, and mapped the genetic variants and gene perturbations of human diseases to NHP cortical cells. This resource establishes a transcriptomic and chromatin accessibility combinatory regulatory landscape at a single-cell and spatially resolved resolution in NHP cortex.
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- 2020
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45. The correlation between UDP-glucuronosyltransferase polymorphisms and environmental endocrine disruptors levels in polycystic ovary syndrome patients
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Liangzhi Xu, Lu Tang, Yunyao Luo, Ying Nie, and Charles C. Xu
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Adult ,Risk ,medicine.medical_specialty ,endocrine system diseases ,Adolescent ,Genotype ,medicine.drug_class ,Single-nucleotide polymorphism ,Endocrine Disruptors ,Polymorphism, Single Nucleotide ,Gas Chromatography-Mass Spectrometry ,03 medical and health sciences ,0302 clinical medicine ,Asian People ,single nucleotide polymorphism ,Internal medicine ,medicine ,Endocrine system ,Humans ,030212 general & internal medicine ,Allele ,Glucuronosyltransferase ,Child ,glucuronosyltransferases ,business.industry ,Case-control study ,General Medicine ,Environmental exposure ,Clinical Trial/Experimental Study ,Environmental Exposure ,Androgen ,Polycystic ovary ,female genital diseases and pregnancy complications ,environmental endocrine disruptors ,Endocrinology ,polycystic ovary syndrome ,030220 oncology & carcinogenesis ,Case-Control Studies ,Female ,business ,Research Article - Abstract
Background: In recent years, there has been an interest in whether environmental endocrine disruptors (EEDs) may contribute to the endocrine disorders in patients with polycystic ovary syndrome (PCOS). The clearance of EEDs from the human body is regulated by the glucuronidation of UDP-glucuronosyltransferases (UGT). This study aimed to analyze the relationship of UGT1A1, UGT2B7, and UGT2B15 polymorphisms with the metabolism of EEDs in patients with PCOS. Methods: A total of 357 Chinese women (119 PCOS cases and 238 controls) were genotyped for polymorphisms of UGT1A1G71R, UGT2B7H268Y, and UGT2B15D85Y. The plasma concentrations of EEDs were measured by the gas chromatography-mass spectrometry method. The association between UGT polymorphisms and the serum level of EEDs in patients with PCOS was analyzed. Results: The UGT2B7H268Y single nucleotide polymorphism was associated with an increased risk of PCOS. The homozygous polymorphism (TT) of UGT2B7H268Y showed higher bisphenol A and PAEs concentrations in serum. However, a single nucleotide polymorphism on UGT2B15D85Y expression was associated with a decreased risk of PCOS. Subjects homozygous for the T allele of UGT2B15D85Y had a significant effect on phthalates in the blood. In addition, our results also showed that the homozygous polymorphism (TT) of UGT2B7H268Y and UGT2B15D85Y was associated with the capacity of the excretion of androgen in patients with PCOS. Conclusions: Our study reported the novel associations between the UGT polymorphisms and EEDs concentrations in patients with PCOS, supporting the relevance of genetic differences in EEDs metabolism, which might be considered as an etiology of PCOS.
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- 2020
46. Effects of metformin on pregnancy outcome, metabolic profile, and sex hormone levels in women with polycystic ovary syndrome and their offspring: a systematic review and meta-analysis
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Daiyu, Zhu, Yan, Chen, Jianfeng, Huang, Hongxia, Deng, Xiaoyang, Shen, Danhua, Lu, and Liangzhi, Xu
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Original Article ,General Medicine - Abstract
BACKGROUND: Researches of the efficacy and safety of metformin on long-term pregnancy outcomes remains conflicted. We performed an updated systematic review and meta-analysis to systematically investigate the effect of metformin treatment on pregnancy outcome, metabolic profile, and sex hormone characteristics in women with polycystic ovary syndrome (PCOS) and their offspring. METHODS: The PubMed, Embase, and Cochrane Library databases were searched from inception to July 10, 2021 with the keywords “metformin”, “PCOS”, and “pregnancy”. Randomized controlled studies reported pregnant related outcomes after metformin intervention among PCOS women were included, while abstracts and reviews were excluded. Two authors independently identified trials, extracted data and assessed risk of bias with Cochrane Reviewer’s Handbook 5.0. Random effects models were used to evaluate the pooled risk ratios (RR) and 95% confidence intervals (95% CI) of pregnancy outcome, metabolic profile, and sex hormone levels. RESULTS: Eighteen studies were included. The majority of trials were in medium methodological quality. In terms of pregnancy complications among women with PCOS, metformin treatment was associated with a significantly reduced risk of preterm delivery (RR =0.37, 95% CI: 0.23–0.61), pregnancy-induced hypertension (PIH) and preeclampsia (RR =0.45, 95% CI: 0.24–0.83) and macrosomia (RR =0.26, 95% CI: 0.11–0.64). In terms of offspring, metformin significantly associated with larger head circumference (MD =0.29, 95% CI: 0.13–0.45) and higher long-term body mass index (BMI) measures (MD =0.37, 95% CI: 0.17–0.56). In terms of metabolic profile and sex hormone characteristics, a significant decrease in homeostatic model assessment for insulin resistance (HOMA2-IR) scores was found in mothers (MD =−0.32, 95% CI: −0.63 to −0.02), whereas a significant increase of sex hormone binding globulin (SHBG) levels was detected in offspring (MD =0.33, 95% CI: 0.01–0.65). DISCUSSION: Although the relative low quality of randomized controlled trials (RCTs) and limited results made it difficult to draw a definite conclusion, our study showed that metformin treatment during pregnancy can reduce the risk of pregnancy complications but may have impacts on increasing SHBG levels and long-term BMI in offspring.
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- 2022
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47. Evaluation of body composition in POF and its association with bone mineral density and sex steroid levels
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Jun Zhang, Zhang Jing, Yaxian Ma, Yunyao Luo, Liangzhi Xu, Xiaoyan Luo, and Ran Cheng
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Adult ,0301 basic medicine ,medicine.medical_specialty ,Waist ,Endocrinology, Diabetes and Metabolism ,Osteoporosis ,Physical exercise ,Primary Ovarian Insufficiency ,Physical strength ,03 medical and health sciences ,0302 clinical medicine ,Endocrinology ,Bone Density ,Internal medicine ,Humans ,Medicine ,Gonadal Steroid Hormones ,Bone mineral ,030219 obstetrics & reproductive medicine ,business.industry ,Obstetrics and Gynecology ,Middle Aged ,medicine.disease ,Circumference ,030104 developmental biology ,Sex steroid ,Case-Control Studies ,Body Composition ,Female ,Composition (visual arts) ,business - Abstract
The study aims to investigate the body composition and bone mineral density (BMD) characteristics and discuss the relationships among body composition, BMD and sex steroid level in POF. A total of 240 POF patients, 240 normal women, and peri/postmenopausal women (Peri-M/Post-M) (260 patients in each group) were included. Compared to the control group, POF patients? strength of left/right lower limb (SLL/SRL), muscle distributing coefficient of lower limbs (MD) decreased however, waist circumference (WC) and hip circumference (HC) increased. The weight, WC, HC, whole body fat percentage (BF%), average fat distribution (FD), MD of POF patients were lower than those among Peri-M and Post-M and BMD were lower than the Peri-M, yet still higher than Post-M. Moreover, BMD were significantly positively correlated with BF%, FD, SLL, MD and estradiol (E2). The factors associated with L2-L4 BMD were E2, SRL, FD and age. For the FN BMD, the factors were FD, E2 and SLL. Therefore, we conclude that maintenance of appropriate weight, physical exercise and hormone replacement treatment (HRT) may have positive effects on increasing BMD, improving muscle mass and muscle strength, preventing osteoporosis.摘要 本研究旨在探讨体成分和骨密度(BMD)特征, 并探讨POF患者体成分、骨密度和性类固醇水平之间的关系。共纳入240名POF患者、240名正常女性和围绝经期/绝经后女性(Peri-M/Post-M)(每组260名患者)。与对照组相比, POF患者左/右下肢力量(SLL/SRL)、下肢肌肉分布系数(MD)下降, 腰围(WC)和臀围(HC)增加。POF患者的体重、WC、HC、全身脂肪百分比(BF%)、平均脂肪分布(FD)、MD均低于Peri-M和Post-M;BMD均低于Peri-M, 即便如此, 仍高于Post-M。此外, BMD与BF%、FD、SLL、MD和雌二醇(E2)显著正相关。与L2-L4 BMD相关的因素是E2、SRL、FD和年龄。对于FN BMD, 相关因素是FD、E2和SLL。因此, 我们得出结论, 维持适当的体重、体育锻炼和激素替代治疗(HRT)可能对增加BMD、改善肌肉质量和肌肉力量、预防骨质疏松症有积极作用。.
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- 2018
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48. Irisin promotes proliferation but inhibits differentiation in osteoclast precursor cells
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Ying Hu, Lin Liu, Ran Cheng, Yunyao Luo, Charles C. Xu, Wenming Xu, Liangzhi Xu, Jun Zhang, Zhang Jing, Yaxian Ma, Ying Nie, Rujun Zeng, Xiaoyong Qiao, and Yang Zhilan
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0301 basic medicine ,Cathepsin ,Cell growth ,Chemistry ,p38 mitogen-activated protein kinases ,Osteoblast ,Biochemistry ,Cell biology ,03 medical and health sciences ,030104 developmental biology ,medicine.anatomical_structure ,Osteoclast ,Precursor cell ,Myokine ,Genetics ,medicine ,Signal transduction ,Molecular Biology ,Biotechnology - Abstract
The receptor activator of NF-κB ligand-induced osteoclast differentiation has a critical role in the process of bone metabolism. Overactivation of osteoclastogenesis may result in a series of diseases. Irisin, a novel myokine, which was first reported in 2012, has been proposed to mediate the beneficial metabolic effects of exercise. Studies have demonstrated that irisin targets osteoblasts by promoting osteoblast proliferation and differentiation; however, the underlying mechanism regarding the effect of irisin on osteoclasts remains elusive. Using 2 types of osteoclast precursor cells, RAW264.7 cells and mouse bone marrow monocytes, we showed that irisin promoted osteoclast precursor cell proliferation but inhibited osteoclast differentiation. Irisin down-regulated the expression of osteoclast differentiation marker genes, including receptor activators of NF-κB, nuclear factor of activated T cells, cytoplasmic 1, cathepsin K, and tartrate-resistant acid phosphatase (TRAP), as well as decreasing the number of TRAP-positive multinucleated cells and hydroxyapatite resorption pits. Furthermore, we showed that irisin suppressed the NF-κB signaling pathway, but activated the p38 and JNK signaling pathways. In the presence of an inhibitor of p38 and JNK, irisin-induced promotion of RAW264.7 cell proliferation was attenuated. However, irisin-induced inhibition of osteoclast differentiation was not affected by either the p38 or JNK signaling pathway. Our study suggested the direct effect of irisin on osteoclastogenesis and revealed the mechanism responsible for the therapeutic potential of irisin in bone metabolism disease.-Ma, Y., Qiao, X., Zeng, R., Cheng, R., Zhang, J., Luo, Y., Nie, Y., Hu, Y., Yang, Z., Zhang, J., Liu, L., Xu, W., Xu, C. C., Xu, L. Irisin promotes proliferation but inhibits differentiation in osteoclast precursor cells.
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- 2018
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49. Irisin level and neonatal birthweight: A systematic review and meta-analysis
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Xijiao Liu, Dan Shan, Li Zou, Yitong Cai, Liangzhi Xu, and Xiaoyong Qiao
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Adult ,medicine.medical_specialty ,Cord ,Weight Gain ,Umbilical cord ,03 medical and health sciences ,0302 clinical medicine ,Pregnancy ,Statistical significance ,Epidemiology ,Myokine ,Medicine ,Birth Weight ,Humans ,030212 general & internal medicine ,030219 obstetrics & reproductive medicine ,business.industry ,Obstetrics ,Infant, Newborn ,Obstetrics and Gynecology ,Gestational age ,medicine.disease ,Fetal Blood ,Pregnancy Complications ,medicine.anatomical_structure ,Reproductive Medicine ,Meta-analysis ,Infant, Small for Gestational Age ,Small for gestational age ,Female ,business - Abstract
Irisin is an important crosstalk myokine between adipose and muscle tissue. Disorders in irisin secretion can lead to fetal growth abnormalities and even lead to metabolic syndromes in adult life. This study aimed to evaluate the association between irisin level in umbilical cord blood and maternal serum with neonatal birthweight. The Preferred Reporting Items for Systematic Reviews and Meta-analysis (PRISMA) statement and the Meta-analysis of Observational Studies in Epidemiology (MOOSE) guideline were followed. A comprehensive search of eight databases (PubMed, Embase, Web of Science, Cochrane Central Register of Controlled Trials, CBM, CNKI, WANFANG and VIP) was performed from inception to November 2019. Studies with original date reporting irisin levels in newborns of small for gestational age (SGA) and newborns of large for gestational age (LGA) were included. Additionally, studies reporting correlation coefficients of irisin with birthweight were analyzed. Newcastle-Ottawa score system and Grading of Recommendations, Assessment, Development and Evaluation (GRADE) approach were applied. Seventeen studies with 1866 participants were included. Pooled analysis indicated decreased cord irisin levels in SGA newborns (MD -10.57, 95 % CI -13.41 to -7.73) and increased irisin levels in LGA newborns (MD 3.80, 95 % CI 1.91–5.70). Umbilical cord irisin level was positively correlated with neonatal birthweight (r = 0.41 95 %CI 0.04 to 0.68). The pooled correlation coefficient of maternal serum irisin with birthweight has no statistical significance. This meta-analysis suggested that the umbilical cord irisin levels were impaired in fetal growth abnormalities. Umbilical cord blood irisin level was positively correlated with birthweight.
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- 2020
50. Placenta membranacea: an anormaly of the placenta: Three case reports
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Lin Wu, Lu Tang, Ying Hu, Liangzhi Xu, Li Zhang, and Qiang Wei
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Adult ,medicine.medical_specialty ,Placenta accreta ,Placenta ,placenta membranacea ,Chorioamnionitis ,Ultrasonography, Prenatal ,ultrasonic diagnosis ,Diagnosis, Differential ,03 medical and health sciences ,Young Adult ,0302 clinical medicine ,Pregnancy ,Medicine ,Humans ,030212 general & internal medicine ,Clinical Case Report ,Ultrasonography, Doppler, Color ,placenta disease ,reproductive and urinary physiology ,Fetus ,Antepartum hemorrhage ,business.industry ,Obstetrics ,Pregnancy Outcome ,General Medicine ,chorionic villi ,medicine.disease ,FGR ,Placenta previa ,Ostium ,medicine.anatomical_structure ,030220 oncology & carcinogenesis ,embryonic structures ,Female ,business ,Research Article - Abstract
Rationale: The placenta membranacea (PM) is a rare type of placental abnormality, which is associated with placenta previa, antepartum hemorrhage (APH), postpartum hemorrhage (PPH), chorioamnionitis, fetal growth restriction (FGR), preterm birth even stillbirth. The purpose of this case report is to summarize the characteristics and analyze the relevant factors of PM. Patients concerns: Repetitive B-ultrasound of the first patient demonstrated a thin placenta covering the most part of uterine wall, which completely covers the internal cervical ostium for 22 weeks. B-ultrasound of the second patient showed placenta partially covering the internal cervical ostium and fetus small for gestation age for 23 days. The third patient complained of abdominal pain and vaginal discharge for 1 day. Diagnoses: Diagnosis of PM is based on Doppler ultrasound apparatus, and confirmed by pathology. Interventions and Outcomes: In the first patient, elective cesarean section was performed. The second patient required termination of pregnancy due to poor postnatal outcome. The third patient underwent intrauterine fetal death. Of these 3 cases, one delivered a term fetus by cesarean section complicated with placenta previa and placenta accreta, one terminated the pregnancy because of serious fetal growth retardation, and the other underwent intrauterine fetal death. Lessons: High-resolution color Doppler ultrasound apparatus can improve the diagnostic accuracy, and close antenatal surveillance followed by proper arrangement of delivery may improve neonatal outcomes.
- Published
- 2019
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