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Background: Little s antigen is mainly defined by a single nucleotide polymorphism at c.143C (p.Thr48) on the GYPB gene. Several variants on GYPB can alter the expression of s antigen. The aim of this study was to investigate the molecular basis of variant s antigen expression in the Chinese population. Study Design and Methods: A total of 4983 whole blood samples were collected to screen the individuals with discrepant s typing results using two different monoclonal anti‐s. Then, the sequence of GYPB exon 4 was analyzed by Sanger sequencing. Flow cytometry analysis was performed to quantify s antigen expression on red blood cells (RBCs). In vitro expression study was performed to verify the effect of the GYPB variants identified on the expression of s antigen. Results: Four donors were identified to have discrepant s typing results. Sanger sequencing showed that three donors carried the c.173C > G variant (p.Pro58Arg) specific for sD antigen, the other one carried a novel GYPB (c.160C > T, p.Arg54Cys) variant. Flow cytometry identified a partial and weak expression of s antigen on the RBCs of the four donors. Furthermore, in vitro expression study confirmed the effect of the two variants on the s antigen expression. Conclusion: The results demonstrated that in addition to p.Thr48, the two extra amino acids p.Arg54 and p.Pro58 are also important for full expression of s antigen. Since the individuals with partial s antigen are at risk for the development of alloanti‐s, it is important to select at least two different monoclonal anti‐s for correct s typing. [ABSTRACT FROM AUTHOR]

In view of problem that negative sequence components in unbalanced coal mine power system affect stable running of electric locomotive, and in order to satisfy control strategy of three-phase PWM rectifier, the characteristics of asymmetric signal under rotating coordinate system and the principle of enhanced notch filter were studied, and a separation method of positive and negative sequence component was presented. The rotating-coordinate system was used to transform the asymmetric signal and the enhanced adaptive notch filter was used to filter the two-times frequency signal in the method. The Matlab/Simulink simulation results show that the method can extract DC value of positive and negative sequence components in asymmetric signal precisely and quickly and can filter some harmonic waves.

The Cr3+-doped near-infrared (NIR) phosphors have been extensively investigated owing to their promising applications in biomedicine, food safety detection, and night vision surveillance. However, achieving broadband (FWHM > 160 nm) NIR emission is still challenging. In this paper, novel Y2Mg2Ga2−xSi2O12:xCr3+ (YMGS:xCr3+, x = 0.005–0.08) phosphors were prepared by a high-temperature solid-state reaction. The crystal structure, photoluminescence properties of the phosphor, and the device performance of a pc-LED were researched in detail. When excited at 440 nm, the YMGS:0.04Cr3+ phosphor exhibited broadband emission in the range of 650–1000 nm, peaking at 790 nm with a full width at half-maximum (FWHM) of up to 180 nm. The wide FWHM of YMGS:Cr3+ is conducive to its extensive application in NIR spectroscopic technology. In addition, the YMGS:0.04Cr3+ phosphor could maintain 70% of the initial emission intensity at 373 K. By combining the commercial blue chip with the YMGS:0.04Cr3+ phosphor, the resulting NIR pc-LED demonstrated a near-infrared output power of 14 mW with a photoelectric conversion efficiency of 5% at a drive current of 100 mA. This work provides a broadband emission NIR phosphor option for NIR pc-LED devices. [ABSTRACT FROM AUTHOR]

For high-efficiency lighting and wide color gamut backlight display, high-quality narrow-band red phosphors for WLEDs are still in high demand. Herein, a novel red-emitting fluoride phosphor Cs2NaGaF6:Mn4+ was successfully synthesized by a simple two-step co-precipitation method and exhibits ultra-intense zero-phonon lines (ZPLs) and long wavelength phonon sidebands under 468 nm blue light irradiation. The ZPL emission peak of Cs2NaGaF6:Mn4+ was located at 627 nm, which is much stronger than its υ6 vibration peak, more matchable with the eye-sensitive region of humans, and beneficial for obtaining higher luminous efficiency of WLEDs. Interestingly, the υ6 vibration peak of this red phosphor is at 636.5 nm, which is larger than that of the common fluoride phosphor A2BF6:Mn4+ (usually at about 630 nm, represented by K2SiF6:Mn4+) at about 6.5 nm. Thanks to the longer wavelength of the υ6 vibration peak, the chromaticity coordinates (0.7026, 0.2910) with a larger x-coordinate value were realized, leading to a potentially wider color gamut of WLEDs. In addition, this phosphor has high thermal stability and its emission intensity at 423 K remains 93.7% of the initial intensity at room temperature. The lumen efficiency of WLED1 packaged with a mixture of Cs2NaGaF6:Mn4+ and YAG:Ce3+ on the InGaN blue chip is 115.7 lm W−1 with the color temperature (Tc) = 3390 K and the colour rendering index (Ra) = 92.5 under 20 mA driving current. The chromaticity coordinates of WLED2 packaged with Cs2NaGaF6:Mn4+ and β-SiAlON:Eu2+ on the InGaN blue chip are (0.3149, 0.3262) and the calculated color gamut is up to 118.4% (NTSC). These results indicate that Cs2NaGaF6:Mn4+ red phosphors have promising applications in the high-quality lighting and display fields. [ABSTRACT FROM AUTHOR]

Near-infrared (NIR) phosphor-converted light emitting diode (pc-LED) light sources have broad application prospects in environmental science, biomedical and plant growth fields. However, NIR phosphors still suffer from narrowband emission and low thermal stability. Here, we prepared a novel Lu2CaMg2−xSi3O12:xCr3+ (LCMS:xCr3+, x = 0.005–0.06) phosphor with broadband emission by a high-temperature solid state reaction. Under excitation at 450 nm, the emission spectrum of the LCMS:0.05Cr3+ phosphor shows a broadband emission in the range of 650–1000 nm with a large full width at half maximum (FWHM) of 125 nm and an R-line emission of 692 nm. In addition, the LCMS:0.05Cr3+ phosphor has good thermal stability and can maintain 70% emission intensity at 150 °C relative to that at room temperature. The LCMS:0.05Cr3+ phosphor exhibits a high internal quantum efficiency (IQE) of ∼76%. Using this phosphor, a NIR pc-LED with photoelectric efficiencies of 14.8% at 100 mA and 6.0% at 320 mA and NIR output powers of 59.5 mW at 100 mA and 181.0 mW at 320 mA was obtained. The broadband LCMS:0.05Cr3+ phosphor with a NIR emission peaked at 750 nm can serve as a light source for plant cultivation and has superior application prospects in the agriculture field. [ABSTRACT FROM AUTHOR]

The Cr 3+ -doped near-infrared (NIR) phosphors have been extensively investigated owing to their promising applications in biomedicine, food safety detection, and night vision surveillance. However, achieving broadband (FWHM > 160 nm) NIR emission is still challenging. In this paper, novel Y 2 Mg 2 Ga 2- x Si 2 O 12 : x Cr 3+ (YMGS: x Cr 3+ , x = 0.005-0.08) phosphors were prepared by a high-temperature solid-state reaction. The crystal structure, photoluminescence properties of the phosphor, and the device performance of a pc-LED were researched in detail. When excited at 440 nm, the YMGS:0.04Cr 3+ phosphor exhibited broadband emission in the range of 650-1000 nm, peaking at 790 nm with a full width at half-maximum (FWHM) of up to 180 nm. The wide FWHM of YMGS:Cr 3+ is conducive to its extensive application in NIR spectroscopic technology. In addition, the YMGS:0.04Cr 3+ phosphor could maintain 70% of the initial emission intensity at 373 K. By combining the commercial blue chip with the YMGS:0.04Cr 3+ phosphor, the resulting NIR pc-LED demonstrated a near-infrared output power of 14 mW with a photoelectric conversion efficiency of 5% at a drive current of 100 mA. This work provides a broadband emission NIR phosphor option for NIR pc-LED devices.

For high-efficiency lighting and wide color gamut backlight display, high-quality narrow-band red phosphors for WLEDs are still in high demand. Herein, a novel red-emitting fluoride phosphor Cs 2 NaGaF 6 :Mn 4+ was successfully synthesized by a simple two-step co-precipitation method and exhibits ultra-intense zero-phonon lines (ZPLs) and long wavelength phonon sidebands under 468 nm blue light irradiation. The ZPL emission peak of Cs 2 NaGaF 6 :Mn 4+ was located at 627 nm, which is much stronger than its υ 6 vibration peak, more matchable with the eye-sensitive region of humans, and beneficial for obtaining higher luminous efficiency of WLEDs. Interestingly, the υ 6 vibration peak of this red phosphor is at 636.5 nm, which is larger than that of the common fluoride phosphor A 2 BF 6 :Mn 4+ (usually at about 630 nm, represented by K 2 SiF 6 :Mn 4+ ) at about 6.5 nm. Thanks to the longer wavelength of the υ 6 vibration peak, the chromaticity coordinates (0.7026, 0.2910) with a larger x -coordinate value were realized, leading to a potentially wider color gamut of WLEDs. In addition, this phosphor has high thermal stability and its emission intensity at 423 K remains 93.7% of the initial intensity at room temperature. The lumen efficiency of WLED1 packaged with a mixture of Cs 2 NaGaF 6 :Mn 4+ and YAG:Ce 3+ on the InGaN blue chip is 115.7 lm W -1 with the color temperature ( T c ) = 3390 K and the colour rendering index ( R a ) = 92.5 under 20 mA driving current. The chromaticity coordinates of WLED2 packaged with Cs 2 NaGaF 6 :Mn 4+ and β-SiAlON:Eu 2+ on the InGaN blue chip are (0.3149, 0.3262) and the calculated color gamut is up to 118.4% (NTSC). These results indicate that Cs 2 NaGaF 6 :Mn 4+ red phosphors have promising applications in the high-quality lighting and display fields.

Near-infrared (NIR) phosphor-converted light emitting diode (pc-LED) light sources have broad application prospects in environmental science, biomedical and plant growth fields. However, NIR phosphors still suffer from narrowband emission and low thermal stability. Here, we prepared a novel Lu2CaMg2-xSi3O12:xCr3+ (LCMS:xCr3+, x = 0.005-0.06) phosphor with broadband emission by a high-temperature solid state reaction. Under excitation at 450 nm, the emission spectrum of the LCMS:0.05Cr3+ phosphor shows a broadband emission in the range of 650-1000 nm with a large full width at half maximum (FWHM) of 125 nm and an R-line emission of 692 nm. In addition, the LCMS:0.05Cr3+ phosphor has good thermal stability and can maintain 70% emission intensity at 150 °C relative to that at room temperature. The LCMS:0.05Cr3+ phosphor exhibits a high internal quantum efficiency (IQE) of ∼76%. Using this phosphor, a NIR pc-LED with photoelectric efficiencies of 14.8% at 100 mA and 6.0% at 320 mA and NIR output powers of 59.5 mW at 100 mA and 181.0 mW at 320 mA was obtained. The broadband LCMS:0.05Cr3+ phosphor with a NIR emission peaked at 750 nm can serve as a light source for plant cultivation and has superior application prospects in the agriculture field.

Compositing with metal oxides is proved to be an efficient strategy to improve electrochemical performance of anode material Li 4 Ti 5 O 12 for lithium-ion batteries. Herein, spherical Li 4 Ti 5 O 12 /NiO composite powders have been successfully prepared via a spray drying method. X-ray diffraction and high-resolution transmission electron microscopy results demonstrate that crystal structure of the powders is spinel. Scanning electron microscopy results show that NiO uniformly distributes throughout Li 4 Ti 5 O 12 matrix. It is found that compositing with NiO increases both discharge platform capacity and rate stability of Li 4 Ti 5 O 12 . The as-prepared Li 4 Ti 5 O 12 /NiO (5%) exhibits a high initial discharge capacity of 381.3 mAh g -1 at 0.1 C, and a discharge capacity of 194.7 mAh g -1 at an ultrahigh rate of 20 C., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2020 Liu, Zhong, Chen, Meng, Wang, Liao and Zhou.)

Objective: To explore the correlation between special A/O genotype and the O phenotype., Methods: Group O samples with partially reduced or lack of isoagglutinins were collected to determinate the ABO genotype with a PCR-sequence specific primer (PCR-SSP) assay. Seven samples with A/O genotype were selected for further study. Serological tests including forward and reverse typing, H antigen determination and adsorption/elution were carried out with a tube method. Genomic DNA was genotyped by amplifying and sequencing of the coding regions of exons 1 to 7 of the ABO gene., Results: Seven samples were serotyped as group O by the forward typing test. However, reduced anti-A activity was found in 5 samples by the reverse typing test, reduced anti-A and anti-B activities were found in 1 sample, and no anti-A isoagglutinin activity was found with 1 sample. H antigen was determined in all samples by routine serologic method. Neither anti-A nor anti-B was eluted from red cells derived from all samples. Three samples were genotyped as Ael02/O02, whilst the remainders were Ael02/O13, Ael02/O65, Am04/O75, Ael06/O02, respectively., Conclusion: Special A/O genotype may not express the A antigen, leading to the generation of group O red cells. Reduced or missed anti-A activity is the typical serological feature of this special group of O phenotype, for which ABO*Ael02 and ABO*O02 are the major alleles. Group O individuals with isoagglutinin detection problem should be grouped by serological tests and genomic DNA analysis.

Objective: To explore the molecular basis for a novel B(A) phenotype., Methods: Genomic DNA was abstracted from peripheral blood sample from the proband. ABO genotyping were carried out with sequence specific primer PCR (PCR-SSP). Exons 6 and 7 of the ABO gene were amplified with PCR and sequenced., Results: Anti-A serum could not be adsorbed or eluted by the donor's red blood cells, and no irregular antibodies were found in the plasma. PCR-SSP showed that the ABO genotype of the donor was ABO *B/O. Sequencing results showed that one of the alleles was ABO *O02, while the other could not be defined but contained the following mutation points, 297A>G, 526C>G, 657C>T, 701C>T, 703G>A, 796C>A, 803G>C, and 930G>A. The data was accepted by the GenBank (the loading code was KM974887) and the Blood Group Antigen Mutation Database, and was confirmed to be a novel allele of B(A)., Conclusion: A novel allele ABO *B(A)07 with 701C>T has been identified, which may facilitate further study on blood antigen variants and typing of the blood groups.