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195 results on '"Liao Wei-Li"'

1. Selected Reaction Monitoring (SRM) Analysis of Epidermal Growth Factor Receptor (EGFR) in Formalin Fixed Tumor Tissue

Author

Hembrough Todd, Thyparambil Sheeno, Liao Wei-Li, Darfler Marlene M, Abdo Joseph, Bengali Kathleen M, Taylor Paul, Tong Jiefei, Lara-Guerra Humberto, Waddell Thomas K, Moran Michael F, Tsao Ming-Sound, Krizman David B, and Burrows Jon

Subjects
Formalin fixed, FFPE, EGFR, Gefitinib, Targeted therapy, Patient tissue, Quantitative, Personalized medicine, Molecular diagnostics, Non-small cell lung cancer, Medicine
Abstract

Abstract Background Analysis of key therapeutic targets such as epidermal growth factor receptor (EGFR) in clinical tissue samples is typically done by immunohistochemistry (IHC) and is only subjectively quantitative through a narrow dynamic range. The development of a standardized, highly-sensitive, linear, and quantitative assay for EGFR for use in patient tumor tissue carries high potential for identifying those patients most likely to benefit from EGFR-targeted therapies. Methods A mass spectrometry-based Selected Reaction Monitoring (SRM) assay for the EGFR protein (EGFR-SRM) was developed utilizing the Liquid Tissue®-SRM technology platform. Tissue culture cells (n = 4) were analyzed by enzyme-linked immunosorbent assay (ELISA) to establish quantitative EGFR levels. Matching formalin fixed cultures were analyzed by the EGFR-SRM assay and benchmarked against immunoassay of the non-fixed cultured cells. Xenograft human tumor tissue (n = 10) of non-small cell lung cancer (NSCLC) origin and NSCLC patient tumor tissue samples (n = 23) were microdissected and the EGFR-SRM assay performed on Liquid Tissue lysates prepared from microdissected tissue. Quantitative curves and linear regression curves for correlation between immunoassay and SRM methodology were developed in Excel. Results The assay was developed for quantitation of a single EGFR tryptic peptide for use in FFPE patient tissue with absolute specificity to uniquely distinguish EGFR from all other proteins including the receptor tyrosine kinases, IGF-1R, cMet, Her2, Her3, and Her4. The assay was analytically validated against a collection of tissue culture cell lines where SRM analysis of the formalin fixed cells accurately reflects EGFR protein levels in matching non-formalin fixed cultures as established by ELISA sandwich immunoassay (R2 = 0.9991). The SRM assay was applied to a collection of FFPE NSCLC xenograft tumors where SRM data range from 305amol/μg to 12,860amol/μg and are consistent with EGFR protein levels in these tumors as previously-reported by western blot and SRM analysis of the matched frozen tissue. In addition, the SRM assay was applied to a collection of histologically-characterized FFPE NSCLC patient tumor tissue where EGFR levels were quantitated from not detected (ND) to 670amol/μg. Conclusions This report describes and evaluates the performance of a robust and reproducible SRM assay designed for measuring EGFR directly in FFPE patient tumor tissue with accuracy at extremely low (attomolar) levels. This assay can be used as part of a complementary or companion diagnostic strategy to support novel therapies currently under development and demonstrates the potential to identify candidates for EGFR-inhibitor therapy, predict treatment outcome, and reveal mechanisms of therapeutic resistance.

Published
2012
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2. Complete Remission of Widely Metastatic Human Epidermal Growth Factor Receptor 2–Amplified Pancreatic Adenocarcinoma After Precision Immune and Targeted Therapy With Description of Sequencing and Organoid Correlates

Author

King, Daniel A., Smith, Amber R., Pineda, Gino, Nakano, Michitaka, Michelini, Flavia, Goedegebuure, S. Peter, Thyparambil, Sheeno, Liao, Wei-Li, McCormick, Aaron, Ju, Jihang, Cioffi, Michele, Zhang, Xiuli, Hundal, Jasreet, Griffith, Malachi, Grandori, Carla, Pollastro, Maddy, Rosati, Rachele, Margossian, Astrid, Chatterjee, Payel, Ainge, Trevor, Flory, Marta, Ocampo, Paolo, Chen, Lee-may, Poultsides, George A., Baron, Ari D., Chang, Daniel T., Herman, Joseph M., Gillanders, William E., Park, Haeseong, Hoos, William A., Nichols, Mike, Fisher, George A., and Kuo, Calvin J.

Published
2023
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4. Targeting wild-type KRAS-amplified gastroesophageal cancer through combined MEK and SHP2 inhibition

Author

Wong, Gabrielle S., Zhou, Jin, Liu, Jie Bin, Wu, Zhong, Xu, Xinsen, Li, Tianxia, Xu, David, Schumacher, Steven E., Puschhof, Jens, McFarland, James, Zou, Charles, Dulak, Austin, Henderson, Les, Xu, Peng, O’Day, Emily, Rendak, Rachel, Liao, Wei-li, Cecchi, Fabiola, Hembrough, Todd, Schwartz, Sarit, Szeto, Christopher, Rustgi, Anil K., Wong, Kwok-Kin, Diehl, J. Alan, Jensen, Karin, Graziano, Francesco, Ruzzo, Annamaria, Fereshetian, Shaunt, Mertins, Philipp, Carr, Steven A., Beroukhim, Rameen, Nakamura, Kenichi, Oki, Eiji, Watanabe, Masayuki, Baba, Hideo, Imamura, Yu, Catenacci, Daniel, and Bass, Adam J.

Published
2018
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5. A comparative study of gastric adenocarcinoma HER2 IHC phenotype and mass spectrometry-based quantification

Author

Xu, Bin, primary, Chen, Hui, additional, Zhang, Jingjing, additional, Cong, Yanghai, additional, Ning, Li, additional, Chen, Limin, additional, Zhang, Yushi, additional, Zhang, Yong, additional, Song, Zhanchun, additional, Meng, Yuan, additional, He, Lianqi, additional, Liao, Wei-li, additional, Lu, Ying, additional, and Zhao, Fengyi, additional

Published
2023
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7. Supplementary Figure 3 from EGFR Blockade Reverts Resistance to KRASG12C Inhibition in Colorectal Cancer

Author

Amodio, Vito, primary, Yaeger, Rona, primary, Arcella, Pamela, primary, Cancelliere, Carlotta, primary, Lamba, Simona, primary, Lorenzato, Annalisa, primary, Arena, Sabrina, primary, Montone, Monica, primary, Mussolin, Benedetta, primary, Bian, Yu, primary, Whaley, Adele, primary, Pinnelli, Marika, primary, Murciano-Goroff, Yonina R., primary, Vakiani, Efsevia, primary, Valeri, Nicola, primary, Liao, Wei-Li, primary, Bhalkikar, Anuja, primary, Thyparambil, Sheeno, primary, Zhao, Hui-Yong, primary, de Stanchina, Elisa, primary, Marsoni, Silvia, primary, Siena, Salvatore, primary, Bertotti, Andrea, primary, Trusolino, Livio, primary, Li, Bob T., primary, Rosen, Neal, primary, Di Nicolantonio, Federica, primary, Bardelli, Alberto, primary, and Misale, Sandra, primary

Published
2023
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8. Supplementary Table 2 from EGFR Blockade Reverts Resistance to KRASG12C Inhibition in Colorectal Cancer

Author

Amodio, Vito, primary, Yaeger, Rona, primary, Arcella, Pamela, primary, Cancelliere, Carlotta, primary, Lamba, Simona, primary, Lorenzato, Annalisa, primary, Arena, Sabrina, primary, Montone, Monica, primary, Mussolin, Benedetta, primary, Bian, Yu, primary, Whaley, Adele, primary, Pinnelli, Marika, primary, Murciano-Goroff, Yonina R., primary, Vakiani, Efsevia, primary, Valeri, Nicola, primary, Liao, Wei-Li, primary, Bhalkikar, Anuja, primary, Thyparambil, Sheeno, primary, Zhao, Hui-Yong, primary, de Stanchina, Elisa, primary, Marsoni, Silvia, primary, Siena, Salvatore, primary, Bertotti, Andrea, primary, Trusolino, Livio, primary, Li, Bob T., primary, Rosen, Neal, primary, Di Nicolantonio, Federica, primary, Bardelli, Alberto, primary, and Misale, Sandra, primary

Published
2023
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9. Data from HER2-Mediated Internalization of Cytotoxic Agents in ERBB2 Amplified or Mutant Lung Cancers

Author

Li, Bob T., primary, Michelini, Flavia, primary, Misale, Sandra, primary, Cocco, Emiliano, primary, Baldino, Laura, primary, Cai, Yanyan, primary, Shifman, Sophie, primary, Tu, Hai-Yan, primary, Myers, Mackenzie L., primary, Xu, Chongrui, primary, Mattar, Marissa, primary, Khodos, Inna, primary, Little, Megan, primary, Qeriqi, Besnik, primary, Weitsman, Gregory, primary, Wilhem, Clare J., primary, Lalani, Alshad S., primary, Diala, Irmina, primary, Freedman, Rachel A., primary, Lin, Nancy U., primary, Solit, David B., primary, Berger, Michael F., primary, Barber, Paul R., primary, Ng, Tony, primary, Offin, Michael, primary, Isbell, James M., primary, Jones, David R., primary, Yu, Helena A., primary, Thyparambil, Sheeno, primary, Liao, Wei-Li, primary, Bhalkikar, Anuja, primary, Cecchi, Fabiola, primary, Hyman, David M., primary, Lewis, Jason S., primary, Buonocore, Darren J., primary, Ho, Alan L., primary, Makker, Vicky, primary, Reis-Filho, Jorge S., primary, Razavi, Pedram, primary, Arcila, Maria E., primary, Kris, Mark G., primary, Poirier, John T., primary, Shen, Ronglai, primary, Tsurutani, Junji, primary, Ulaner, Gary A., primary, de Stanchina, Elisa, primary, Rosen, Neal, primary, Rudin, Charles M., primary, and Scaltriti, Maurizio, primary

Published
2023
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10. Supplementary Table 1 from HER2-Mediated Internalization of Cytotoxic Agents in ERBB2 Amplified or Mutant Lung Cancers

Author

Li, Bob T., primary, Michelini, Flavia, primary, Misale, Sandra, primary, Cocco, Emiliano, primary, Baldino, Laura, primary, Cai, Yanyan, primary, Shifman, Sophie, primary, Tu, Hai-Yan, primary, Myers, Mackenzie L., primary, Xu, Chongrui, primary, Mattar, Marissa, primary, Khodos, Inna, primary, Little, Megan, primary, Qeriqi, Besnik, primary, Weitsman, Gregory, primary, Wilhem, Clare J., primary, Lalani, Alshad S., primary, Diala, Irmina, primary, Freedman, Rachel A., primary, Lin, Nancy U., primary, Solit, David B., primary, Berger, Michael F., primary, Barber, Paul R., primary, Ng, Tony, primary, Offin, Michael, primary, Isbell, James M., primary, Jones, David R., primary, Yu, Helena A., primary, Thyparambil, Sheeno, primary, Liao, Wei-Li, primary, Bhalkikar, Anuja, primary, Cecchi, Fabiola, primary, Hyman, David M., primary, Lewis, Jason S., primary, Buonocore, Darren J., primary, Ho, Alan L., primary, Makker, Vicky, primary, Reis-Filho, Jorge S., primary, Razavi, Pedram, primary, Arcila, Maria E., primary, Kris, Mark G., primary, Poirier, John T., primary, Shen, Ronglai, primary, Tsurutani, Junji, primary, Ulaner, Gary A., primary, de Stanchina, Elisa, primary, Rosen, Neal, primary, Rudin, Charles M., primary, and Scaltriti, Maurizio, primary

Published
2023
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11. Supplementary Figure 2 from EGFR Blockade Reverts Resistance to KRASG12C Inhibition in Colorectal Cancer

Author

Amodio, Vito, primary, Yaeger, Rona, primary, Arcella, Pamela, primary, Cancelliere, Carlotta, primary, Lamba, Simona, primary, Lorenzato, Annalisa, primary, Arena, Sabrina, primary, Montone, Monica, primary, Mussolin, Benedetta, primary, Bian, Yu, primary, Whaley, Adele, primary, Pinnelli, Marika, primary, Murciano-Goroff, Yonina R., primary, Vakiani, Efsevia, primary, Valeri, Nicola, primary, Liao, Wei-Li, primary, Bhalkikar, Anuja, primary, Thyparambil, Sheeno, primary, Zhao, Hui-Yong, primary, de Stanchina, Elisa, primary, Marsoni, Silvia, primary, Siena, Salvatore, primary, Bertotti, Andrea, primary, Trusolino, Livio, primary, Li, Bob T., primary, Rosen, Neal, primary, Di Nicolantonio, Federica, primary, Bardelli, Alberto, primary, and Misale, Sandra, primary

Published
2023
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12. Data from EGFR Blockade Reverts Resistance to KRASG12C Inhibition in Colorectal Cancer

Author

Amodio, Vito, primary, Yaeger, Rona, primary, Arcella, Pamela, primary, Cancelliere, Carlotta, primary, Lamba, Simona, primary, Lorenzato, Annalisa, primary, Arena, Sabrina, primary, Montone, Monica, primary, Mussolin, Benedetta, primary, Bian, Yu, primary, Whaley, Adele, primary, Pinnelli, Marika, primary, Murciano-Goroff, Yonina R., primary, Vakiani, Efsevia, primary, Valeri, Nicola, primary, Liao, Wei-Li, primary, Bhalkikar, Anuja, primary, Thyparambil, Sheeno, primary, Zhao, Hui-Yong, primary, de Stanchina, Elisa, primary, Marsoni, Silvia, primary, Siena, Salvatore, primary, Bertotti, Andrea, primary, Trusolino, Livio, primary, Li, Bob T., primary, Rosen, Neal, primary, Di Nicolantonio, Federica, primary, Bardelli, Alberto, primary, and Misale, Sandra, primary

Published
2023
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13. Supplementary Fig 3 from HER2-Mediated Internalization of Cytotoxic Agents in ERBB2 Amplified or Mutant Lung Cancers

Author

Li, Bob T., primary, Michelini, Flavia, primary, Misale, Sandra, primary, Cocco, Emiliano, primary, Baldino, Laura, primary, Cai, Yanyan, primary, Shifman, Sophie, primary, Tu, Hai-Yan, primary, Myers, Mackenzie L., primary, Xu, Chongrui, primary, Mattar, Marissa, primary, Khodos, Inna, primary, Little, Megan, primary, Qeriqi, Besnik, primary, Weitsman, Gregory, primary, Wilhem, Clare J., primary, Lalani, Alshad S., primary, Diala, Irmina, primary, Freedman, Rachel A., primary, Lin, Nancy U., primary, Solit, David B., primary, Berger, Michael F., primary, Barber, Paul R., primary, Ng, Tony, primary, Offin, Michael, primary, Isbell, James M., primary, Jones, David R., primary, Yu, Helena A., primary, Thyparambil, Sheeno, primary, Liao, Wei-Li, primary, Bhalkikar, Anuja, primary, Cecchi, Fabiola, primary, Hyman, David M., primary, Lewis, Jason S., primary, Buonocore, Darren J., primary, Ho, Alan L., primary, Makker, Vicky, primary, Reis-Filho, Jorge S., primary, Razavi, Pedram, primary, Arcila, Maria E., primary, Kris, Mark G., primary, Poirier, John T., primary, Shen, Ronglai, primary, Tsurutani, Junji, primary, Ulaner, Gary A., primary, de Stanchina, Elisa, primary, Rosen, Neal, primary, Rudin, Charles M., primary, and Scaltriti, Maurizio, primary

Published
2023
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14. Supplementary Figure 4 from EGFR Blockade Reverts Resistance to KRASG12C Inhibition in Colorectal Cancer

Author

Amodio, Vito, primary, Yaeger, Rona, primary, Arcella, Pamela, primary, Cancelliere, Carlotta, primary, Lamba, Simona, primary, Lorenzato, Annalisa, primary, Arena, Sabrina, primary, Montone, Monica, primary, Mussolin, Benedetta, primary, Bian, Yu, primary, Whaley, Adele, primary, Pinnelli, Marika, primary, Murciano-Goroff, Yonina R., primary, Vakiani, Efsevia, primary, Valeri, Nicola, primary, Liao, Wei-Li, primary, Bhalkikar, Anuja, primary, Thyparambil, Sheeno, primary, Zhao, Hui-Yong, primary, de Stanchina, Elisa, primary, Marsoni, Silvia, primary, Siena, Salvatore, primary, Bertotti, Andrea, primary, Trusolino, Livio, primary, Li, Bob T., primary, Rosen, Neal, primary, Di Nicolantonio, Federica, primary, Bardelli, Alberto, primary, and Misale, Sandra, primary

Published
2023
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15. Supplementary Fig 1 from HER2-Mediated Internalization of Cytotoxic Agents in ERBB2 Amplified or Mutant Lung Cancers

Author

Li, Bob T., primary, Michelini, Flavia, primary, Misale, Sandra, primary, Cocco, Emiliano, primary, Baldino, Laura, primary, Cai, Yanyan, primary, Shifman, Sophie, primary, Tu, Hai-Yan, primary, Myers, Mackenzie L., primary, Xu, Chongrui, primary, Mattar, Marissa, primary, Khodos, Inna, primary, Little, Megan, primary, Qeriqi, Besnik, primary, Weitsman, Gregory, primary, Wilhem, Clare J., primary, Lalani, Alshad S., primary, Diala, Irmina, primary, Freedman, Rachel A., primary, Lin, Nancy U., primary, Solit, David B., primary, Berger, Michael F., primary, Barber, Paul R., primary, Ng, Tony, primary, Offin, Michael, primary, Isbell, James M., primary, Jones, David R., primary, Yu, Helena A., primary, Thyparambil, Sheeno, primary, Liao, Wei-Li, primary, Bhalkikar, Anuja, primary, Cecchi, Fabiola, primary, Hyman, David M., primary, Lewis, Jason S., primary, Buonocore, Darren J., primary, Ho, Alan L., primary, Makker, Vicky, primary, Reis-Filho, Jorge S., primary, Razavi, Pedram, primary, Arcila, Maria E., primary, Kris, Mark G., primary, Poirier, John T., primary, Shen, Ronglai, primary, Tsurutani, Junji, primary, Ulaner, Gary A., primary, de Stanchina, Elisa, primary, Rosen, Neal, primary, Rudin, Charles M., primary, and Scaltriti, Maurizio, primary

Published
2023
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16. Supplementary Fig 4 from HER2-Mediated Internalization of Cytotoxic Agents in ERBB2 Amplified or Mutant Lung Cancers

Author

Li, Bob T., primary, Michelini, Flavia, primary, Misale, Sandra, primary, Cocco, Emiliano, primary, Baldino, Laura, primary, Cai, Yanyan, primary, Shifman, Sophie, primary, Tu, Hai-Yan, primary, Myers, Mackenzie L., primary, Xu, Chongrui, primary, Mattar, Marissa, primary, Khodos, Inna, primary, Little, Megan, primary, Qeriqi, Besnik, primary, Weitsman, Gregory, primary, Wilhem, Clare J., primary, Lalani, Alshad S., primary, Diala, Irmina, primary, Freedman, Rachel A., primary, Lin, Nancy U., primary, Solit, David B., primary, Berger, Michael F., primary, Barber, Paul R., primary, Ng, Tony, primary, Offin, Michael, primary, Isbell, James M., primary, Jones, David R., primary, Yu, Helena A., primary, Thyparambil, Sheeno, primary, Liao, Wei-Li, primary, Bhalkikar, Anuja, primary, Cecchi, Fabiola, primary, Hyman, David M., primary, Lewis, Jason S., primary, Buonocore, Darren J., primary, Ho, Alan L., primary, Makker, Vicky, primary, Reis-Filho, Jorge S., primary, Razavi, Pedram, primary, Arcila, Maria E., primary, Kris, Mark G., primary, Poirier, John T., primary, Shen, Ronglai, primary, Tsurutani, Junji, primary, Ulaner, Gary A., primary, de Stanchina, Elisa, primary, Rosen, Neal, primary, Rudin, Charles M., primary, and Scaltriti, Maurizio, primary

Published
2023
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17. Supplementary Fig 6 from HER2-Mediated Internalization of Cytotoxic Agents in ERBB2 Amplified or Mutant Lung Cancers

Author

Li, Bob T., primary, Michelini, Flavia, primary, Misale, Sandra, primary, Cocco, Emiliano, primary, Baldino, Laura, primary, Cai, Yanyan, primary, Shifman, Sophie, primary, Tu, Hai-Yan, primary, Myers, Mackenzie L., primary, Xu, Chongrui, primary, Mattar, Marissa, primary, Khodos, Inna, primary, Little, Megan, primary, Qeriqi, Besnik, primary, Weitsman, Gregory, primary, Wilhem, Clare J., primary, Lalani, Alshad S., primary, Diala, Irmina, primary, Freedman, Rachel A., primary, Lin, Nancy U., primary, Solit, David B., primary, Berger, Michael F., primary, Barber, Paul R., primary, Ng, Tony, primary, Offin, Michael, primary, Isbell, James M., primary, Jones, David R., primary, Yu, Helena A., primary, Thyparambil, Sheeno, primary, Liao, Wei-Li, primary, Bhalkikar, Anuja, primary, Cecchi, Fabiola, primary, Hyman, David M., primary, Lewis, Jason S., primary, Buonocore, Darren J., primary, Ho, Alan L., primary, Makker, Vicky, primary, Reis-Filho, Jorge S., primary, Razavi, Pedram, primary, Arcila, Maria E., primary, Kris, Mark G., primary, Poirier, John T., primary, Shen, Ronglai, primary, Tsurutani, Junji, primary, Ulaner, Gary A., primary, de Stanchina, Elisa, primary, Rosen, Neal, primary, Rudin, Charles M., primary, and Scaltriti, Maurizio, primary

Published
2023
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18. Supplementary Table 3 from EGFR Blockade Reverts Resistance to KRASG12C Inhibition in Colorectal Cancer

Author

Amodio, Vito, primary, Yaeger, Rona, primary, Arcella, Pamela, primary, Cancelliere, Carlotta, primary, Lamba, Simona, primary, Lorenzato, Annalisa, primary, Arena, Sabrina, primary, Montone, Monica, primary, Mussolin, Benedetta, primary, Bian, Yu, primary, Whaley, Adele, primary, Pinnelli, Marika, primary, Murciano-Goroff, Yonina R., primary, Vakiani, Efsevia, primary, Valeri, Nicola, primary, Liao, Wei-Li, primary, Bhalkikar, Anuja, primary, Thyparambil, Sheeno, primary, Zhao, Hui-Yong, primary, de Stanchina, Elisa, primary, Marsoni, Silvia, primary, Siena, Salvatore, primary, Bertotti, Andrea, primary, Trusolino, Livio, primary, Li, Bob T., primary, Rosen, Neal, primary, Di Nicolantonio, Federica, primary, Bardelli, Alberto, primary, and Misale, Sandra, primary

Published
2023
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19. Supplementary Fig 5 from HER2-Mediated Internalization of Cytotoxic Agents in ERBB2 Amplified or Mutant Lung Cancers

Author

Li, Bob T., primary, Michelini, Flavia, primary, Misale, Sandra, primary, Cocco, Emiliano, primary, Baldino, Laura, primary, Cai, Yanyan, primary, Shifman, Sophie, primary, Tu, Hai-Yan, primary, Myers, Mackenzie L., primary, Xu, Chongrui, primary, Mattar, Marissa, primary, Khodos, Inna, primary, Little, Megan, primary, Qeriqi, Besnik, primary, Weitsman, Gregory, primary, Wilhem, Clare J., primary, Lalani, Alshad S., primary, Diala, Irmina, primary, Freedman, Rachel A., primary, Lin, Nancy U., primary, Solit, David B., primary, Berger, Michael F., primary, Barber, Paul R., primary, Ng, Tony, primary, Offin, Michael, primary, Isbell, James M., primary, Jones, David R., primary, Yu, Helena A., primary, Thyparambil, Sheeno, primary, Liao, Wei-Li, primary, Bhalkikar, Anuja, primary, Cecchi, Fabiola, primary, Hyman, David M., primary, Lewis, Jason S., primary, Buonocore, Darren J., primary, Ho, Alan L., primary, Makker, Vicky, primary, Reis-Filho, Jorge S., primary, Razavi, Pedram, primary, Arcila, Maria E., primary, Kris, Mark G., primary, Poirier, John T., primary, Shen, Ronglai, primary, Tsurutani, Junji, primary, Ulaner, Gary A., primary, de Stanchina, Elisa, primary, Rosen, Neal, primary, Rudin, Charles M., primary, and Scaltriti, Maurizio, primary

Published
2023
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20. Supplementary Table 1 from EGFR Blockade Reverts Resistance to KRASG12C Inhibition in Colorectal Cancer

Author

Amodio, Vito, primary, Yaeger, Rona, primary, Arcella, Pamela, primary, Cancelliere, Carlotta, primary, Lamba, Simona, primary, Lorenzato, Annalisa, primary, Arena, Sabrina, primary, Montone, Monica, primary, Mussolin, Benedetta, primary, Bian, Yu, primary, Whaley, Adele, primary, Pinnelli, Marika, primary, Murciano-Goroff, Yonina R., primary, Vakiani, Efsevia, primary, Valeri, Nicola, primary, Liao, Wei-Li, primary, Bhalkikar, Anuja, primary, Thyparambil, Sheeno, primary, Zhao, Hui-Yong, primary, de Stanchina, Elisa, primary, Marsoni, Silvia, primary, Siena, Salvatore, primary, Bertotti, Andrea, primary, Trusolino, Livio, primary, Li, Bob T., primary, Rosen, Neal, primary, Di Nicolantonio, Federica, primary, Bardelli, Alberto, primary, and Misale, Sandra, primary

Published
2023
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21. Supplementary Figure 1 from EGFR Blockade Reverts Resistance to KRASG12C Inhibition in Colorectal Cancer

Author

Amodio, Vito, primary, Yaeger, Rona, primary, Arcella, Pamela, primary, Cancelliere, Carlotta, primary, Lamba, Simona, primary, Lorenzato, Annalisa, primary, Arena, Sabrina, primary, Montone, Monica, primary, Mussolin, Benedetta, primary, Bian, Yu, primary, Whaley, Adele, primary, Pinnelli, Marika, primary, Murciano-Goroff, Yonina R., primary, Vakiani, Efsevia, primary, Valeri, Nicola, primary, Liao, Wei-Li, primary, Bhalkikar, Anuja, primary, Thyparambil, Sheeno, primary, Zhao, Hui-Yong, primary, de Stanchina, Elisa, primary, Marsoni, Silvia, primary, Siena, Salvatore, primary, Bertotti, Andrea, primary, Trusolino, Livio, primary, Li, Bob T., primary, Rosen, Neal, primary, Di Nicolantonio, Federica, primary, Bardelli, Alberto, primary, and Misale, Sandra, primary

Published
2023
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22. Supplementary Fig 2 from HER2-Mediated Internalization of Cytotoxic Agents in ERBB2 Amplified or Mutant Lung Cancers

Author

Li, Bob T., primary, Michelini, Flavia, primary, Misale, Sandra, primary, Cocco, Emiliano, primary, Baldino, Laura, primary, Cai, Yanyan, primary, Shifman, Sophie, primary, Tu, Hai-Yan, primary, Myers, Mackenzie L., primary, Xu, Chongrui, primary, Mattar, Marissa, primary, Khodos, Inna, primary, Little, Megan, primary, Qeriqi, Besnik, primary, Weitsman, Gregory, primary, Wilhem, Clare J., primary, Lalani, Alshad S., primary, Diala, Irmina, primary, Freedman, Rachel A., primary, Lin, Nancy U., primary, Solit, David B., primary, Berger, Michael F., primary, Barber, Paul R., primary, Ng, Tony, primary, Offin, Michael, primary, Isbell, James M., primary, Jones, David R., primary, Yu, Helena A., primary, Thyparambil, Sheeno, primary, Liao, Wei-Li, primary, Bhalkikar, Anuja, primary, Cecchi, Fabiola, primary, Hyman, David M., primary, Lewis, Jason S., primary, Buonocore, Darren J., primary, Ho, Alan L., primary, Makker, Vicky, primary, Reis-Filho, Jorge S., primary, Razavi, Pedram, primary, Arcila, Maria E., primary, Kris, Mark G., primary, Poirier, John T., primary, Shen, Ronglai, primary, Tsurutani, Junji, primary, Ulaner, Gary A., primary, de Stanchina, Elisa, primary, Rosen, Neal, primary, Rudin, Charles M., primary, and Scaltriti, Maurizio, primary

Published
2023
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28. Deviation from the precisely timed age-associated patterns revealed by blood metabolomics to find CRC patients at risk of relapse at the CRC diagnosis.

Author

Thyparambil, Sheeno P., primary, Zhu, Xiurui, additional, Zhang, Yani, additional, Sun, Hui, additional, Peng, Junjie, additional, Cai, Sanjun, additional, Li, Yaqi, additional, Fu, Chen, additional, Bao, Pingping, additional, Hao, Shiying, additional, Li, Zhen, additional, Ding, Yun, additional, Yao, Xiaoming, additional, Liao, Wei-Li, additional, Heaton, Robert, additional, Han, Zhi, additional, Tian, Lu, additional, Schilling, James, additional, Sylvester, Karl G., additional, and Ling, Xuefeng, additional

Published
2022
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29. Complete remission in a patient with widely metastatic HER2-amplified pancreatic adenocarcinoma following multimodal therapy informed by tumor sequencing and organoid profiling

Author

King, Daniel A, primary, Smith, Amber R, additional, Pineda, Gino, additional, Nakano, Michitaka, additional, Michelini, Flavia, additional, Goedegebuure, S. Peter, additional, Thyparambil, Sheeno, additional, Liao, Wei-Li, additional, McCormick, Aaron, additional, Ju, Jihang, additional, Cioffi, Michele, additional, Zhang, Xiuli, additional, Hundal, Jasreet, additional, Griffith, Malachi, additional, Grandori, Carla, additional, Pollastro, Maddy, additional, Rosati, Rachele, additional, Margossian, Astrid, additional, Chatterjee, Payel, additional, Ainge, Trevor, additional, Flory, Marta, additional, Ocampo, Paolo, additional, Chen, Lee-may, additional, Poultsides, George A, additional, Baron, Ari D, additional, Chang, Daniel T, additional, Herman, Joseph M, additional, Gillanders, William E, additional, Park, Haeseong, additional, Hoos, William A, additional, Nichols, Mike, additional, Fisher, George A, additional, and Kuo, Calvin J, additional

Published
2021
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30. Early-pregnancy prediction of risk for pre-eclampsia using maternal blood leptin/ceramide ratio: discovery and confirmation

Author

Huang, Qianyang, primary, Hao, Shiying, additional, You, Jin, additional, Yao, Xiaoming, additional, Li, Zhen, additional, Schilling, James, additional, Thyparambil, Sheeno, additional, Liao, Wei-Li, additional, Zhou, Xin, additional, Mo, Lihong, additional, Ladella, Subhashini, additional, Davies-Balch, Shantay R, additional, Zhao, Hangyi, additional, Fan, David, additional, Whitin, John C, additional, Cohen, Harvey J, additional, McElhinney, Doff B, additional, Wong, Ronald J, additional, Shaw, Gary M, additional, Stevenson, David K, additional, Sylvester, Karl G, additional, and Ling, Xuefeng B, additional

Published
2021
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32. Optimizing the conditions of a multiple reaction monitoring assay for membrane proteins: quantification of cytochrome P450 11a1 and adrenodoxin reductase in bovine adrenal cortex and retina

Author

Liao, Wei-Li, Heo, Gun-Young, Dodder, Nathan G., Pikuleva, Irina A., and Turko, Illarion V.

Subjects
Cytochrome P-450 -- Properties, Mathematical optimization -- Research, Membrane proteins -- Properties, Biological assay -- Methods, Chemistry
Abstract

Approximately 30% of naturally occurring proteins are predicted to be embedded in biological membranes. Nevertheless, this group of proteins is traditionally understudied due to limitations of the available analytical tools. To facilitate the analysis of membrane proteins, the analytical methods for their soluble counterparts must be optimized or modified. Multiple reaction monitoring (MRM) assays have proven successful for the absolute quantification of proteins and for profiling protein modifications in ceil lysates and human plasma/serum but have found little application in the analysis of membrane proteins. We report on the optimization of sample preparation conditions for the quantification of two membrane proteins, cytochrome P450 11A1 (CYP11A1) and adrenodoxin reductase (AdR). These conditions can be used for the analysis of other membrane proteins. We have demonstrated that membrane proteins that are lightly associated with the membrane, such as CYP11A1, can be quantified in the total tissue membrane pellet obtained after high-speed centrifugation, whereas proteins that are weakly associated with the membrane, such as AdR, must be quantified in the whole tissue homogenate. We have compared quantifications of CYPIIA1 using two different detergents, RapiGest SP and sodium cholate, and two different trypsins, sequencing grade modified trypsin and trypsin, type IX-S from porcine pancreas. The measured concentrations in these experiments were similar and encouraged the use of either combination of detergent/trypsin for quantification of other membrane proteins. Overall, the CYPllA1 and AdR quantified in rids work ranged from 110 pmol to 10 fmol per milligram of tissue protein. 10.1021/ac100811x

Published
2010

34. Multi-omics longitudinal analyses in stages I to III CRC patients: Surveillance liquid biopsy test to predict early recurrence and enable risk-stratified postoperative CRC management.

Author

Liu, Xuanhui, primary, Zhang, Yani, additional, Zhu, Xiurui, additional, Thyparambil, Sheeno P., additional, Liao, Wei-Li, additional, Zheng, Xiao-bin, additional, You, Jin, additional, Masood, Ashiq, additional, Li, Zhen, additional, Yang, Gabriel, additional, Yao, Xiaoming, additional, Hao, Shiying, additional, Heaton, Robert, additional, Schilling, James, additional, Sylvester, Karl G., additional, Liao, Jiayu, additional, Gao, Feng, additional, Lan, Ping, additional, Ling, Xuefeng, additional, and Wu, Xiaojian, additional

Published
2021
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36. Case finding of early pregnancies at risk of preeclampsia using maternal blood leptin/ceramide ratio: multi-omics discovery and validation from a longitudinal study

Author

Huang, Qianyang, primary, Hao, Shiying, additional, You, Jin, additional, Yao, Xiaoming, additional, Li, Zhen, additional, Schilling, James, additional, Thyparambil, Sheeno, additional, Liao, Wei-Li, additional, Zhou, Xin, additional, Mo, Lihong, additional, Ladella, Subhashini, additional, Fan, David, additional, Whitin, John, additional, Cohen, Harvey, additional, McElhinney, Doff, additional, Wong, Ronald, additional, Shaw, Gary, additional, Stevenson, David, additional, Sylvester, Karl, additional, and Ling, Xuefeng, additional

Published
2021
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37. Targeted multiplex proteomics (TMP) and genomics of early-onset colorectal cancer (EO-CRC).

Author

Kam, Audrey E., primary, Khaliq, Ateeq M., additional, Alam, Nida, additional, Hayden, Dana, additional, Bhama, Anuradha R., additional, Govekar, Henry, additional, Pappas, Sam, additional, Ritz, Ethan M., additional, Singh, Ajaypal, additional, Thyparambil, Sheeno P., additional, Liao, Wei-Li, additional, Bhalkikar, Anuja, additional, Ling, Xuefeng B., additional, Levy, Mia Alyce, additional, Kuzel, Timothy, additional, and Masood, Ashiq, additional

Published
2021
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38. Flanking direct repeats of hisG alter URA3 marker expression at the HWP1 locus of Candida albicans

Author

Sharkey, Laura L., Liao, Wei-li, Ghosh, Anup K., and Fonzi, William A.

Subjects
Gene mutations -- Research, Gene expression -- Research, Candida albicans -- Genetic aspects, Biological sciences
Abstract

HWP1 encodes an adhesin of Candida albicans and has been implicated in filamentation and virulence. URA3, an often-used transformation selection marker, is apparently incorrectly expressed when integrated at the HWP1 locus, which results in an attenuated virulence phenotype. Expression of URA3 is compromised by ectopic integration at other loci as well. In contrast, prior studies from the authors' laboratory had demonstrated that the filamentation deficiency and attenuated virulence of hwp1[DELTA] mutants were fully restored in rescued strains in which URA3 was integrated at the HWP1 locus. This discrepancy prompted a reinvestigation of these mutants. A series of congenic strains were constructed which demonstrated that the filamentation and virulence defects of a homozygous hwp1[DELTA] mutant could be rescued without introduction of a functional HWP1 allele. Despite the absence of detectable differences in URA3 expression, analysis of suppressor mutations suggested that reduced URA3 expression gave rise to the mutant phenotypes. Several independent spontaneous suppressor mutations that restored filamentation to strains of genotype hwp1[DELTA]::hisG-URA3-hisG/hwp1[DELTA]::hisG had acquired a tandem duplication of the hisG-URA3-hisG marker cassette. The hwp1 null mutant and rescued strains differed by the presence or absence of flanking hisG sequence. Substitution of the hisG-URA3-hisG insert of the hwp1 null mutant with URA3 alone largely rescued the filamentation and virulence phenotypes. The presence of a single copy of hisG adjacent to URA3 had no effect. It is concluded that flanking direct repeats of hisG, present as part of a recyclable disruption cassette, negatively influenced URA3 expression and are responsible for the previously reported phenotypes of the hwp1 mutants.

Published
2005

39. Case finding of early pregnancies at risk of preeclampsia using maternal blood leptin/ceramide ratio: multi-omics discovery and validation from a longitudinal study

Author

Huang, Qianyang, primary, Hao, Shiying, additional, You, Jin, additional, Yao, Xiaoming, additional, Li, Zhen, additional, Schilling, James, additional, Thyparambil, Sheeno, additional, Liao, Wei-li, additional, Zhou, Xin, additional, Mo, Lihong, additional, Ladella, Subhashini, additional, Fan, David, additional, Whitin, John C., additional, Cohen, Harvey J., additional, McElhinney, Doff B., additional, Wong, Ronald J., additional, Shaw, Gary M., additional, Stevenson, David K., additional, Sylvester, Karl G., additional, and Ling, Xuefeng B., additional

Published
2020
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43. EGFR Blockade Reverts Resistance to KRASG12C Inhibition in Colorectal Cancer

Author

Amodio, Vito, primary, Yaeger, Rona, additional, Arcella, Pamela, additional, Cancelliere, Carlotta, additional, Lamba, Simona, additional, Lorenzato, Annalisa, additional, Arena, Sabrina, additional, Montone, Monica, additional, Mussolin, Benedetta, additional, Bian, Yu, additional, Whaley, Adele, additional, Pinnelli, Marika, additional, Murciano-Goroff, Yonina R., additional, Vakiani, Efsevia, additional, Valeri, Nicola, additional, Liao, Wei-Li, additional, Bhalkikar, Anuja, additional, Thyparambil, Sheeno, additional, Zhao, Hui-Yong, additional, de Stanchina, Elisa, additional, Marsoni, Silvia, additional, Siena, Salvatore, additional, Bertotti, Andrea, additional, Trusolino, Livio, additional, Li, Bob T., additional, Rosen, Neal, additional, Di Nicolantonio, Federica, additional, Bardelli, Alberto, additional, and Misale, Sandra, additional

Published
2020
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45. HER2-Mediated Internalization of Cytotoxic Agents inERBB2Amplified or Mutant Lung Cancers

Author

Li, Bob T., primary, Michelini, Flavia, additional, Misale, Sandra, additional, Cocco, Emiliano, additional, Baldino, Laura, additional, Cai, Yanyan, additional, Shifman, Sophie, additional, Tu, Hai-Yan, additional, Myers, Mackenzie L., additional, Xu, Chongrui, additional, Mattar, Marissa, additional, Khodos, Inna, additional, Little, Megan, additional, Qeriqi, Besnik, additional, Weitsman, Gregory, additional, Wilhem, Clare J., additional, Lalani, Alshad S., additional, Diala, Irmina, additional, Freedman, Rachel A., additional, Lin, Nancy U., additional, Solit, David B., additional, Berger, Michael F., additional, Barber, Paul R., additional, Ng, Tony, additional, Offin, Michael, additional, Isbell, James M., additional, Jones, David R., additional, Yu, Helena A., additional, Thyparambil, Sheeno, additional, Liao, Wei-Li, additional, Bhalkikar, Anuja, additional, Cecchi, Fabiola, additional, Hyman, David M., additional, Lewis, Jason S., additional, Buonocore, Darren J., additional, Ho, Alan L., additional, Makker, Vicky, additional, Reis-Filho, Jorge S., additional, Razavi, Pedram, additional, Arcila, Maria E., additional, Kris, Mark G., additional, Poirier, John T., additional, Shen, Ronglai, additional, Tsurutani, Junji, additional, Ulaner, Gary A., additional, de Stanchina, Elisa, additional, Rosen, Neal, additional, Rudin, Charles M., additional, and Scaltriti, Maurizio, additional

Published
2020
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49. Translation and evaluation of a pre-clinical 5-protein response prediction signature in a breast cancer phase Ib clinical trial

Author

Ducret, Axel, primary, James, Ian, additional, Wilson, Sabine, additional, Feilke, Martina, additional, Tebbe, Andreas, additional, Dybowski, Nikolaj, additional, Elschenbroich, Sarah, additional, Klammer, Martin, additional, Blackler, Adele, additional, Liao, Wei-Li, additional, Tian, Yuan, additional, Friess, Thomas, additional, Bossenmaier, Birgit, additional, Dietmann, Gabriele, additional, Schaab, Christoph, additional, Hembrough, Todd, additional, and Ceppi, Maurizio, additional

Published
2019
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