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216 results on '"Libera, S."'

26. Nine-year nationwide environmental surveillance of hepatitis E virus in urban wastewaters in Italy (2011–2019)

Author

Iaconelli, M., Ferraro, G. B., Mancini, P., Suffredini, E., Veneri, C., Ciccaglione, A. R., Bruni, R., Libera, S. D., Bignami, F., Brambilla, M., De Medici, D., Brandtner, D., Schembri, P., D&apos, amato, S., and Rosa, G. L.

Subjects
Hepatitis E virus, PCR, Prevalence, Sequencing, Sewage, Typing, Environmental Monitoring, Humans, Italy, Phylogeny, RNA, Viral, Hepatitis E, Waste Water
Published
2020

28. Enteric viruses, somatic coliphages and Vibrio species in marine bathing and non-bathing waters in Italy

Author

Bonadonna, L., primary, Briancesco, R., additional, Suffredini, E., additional, Coccia, A., additional, Della Libera, S., additional, Carducci, A., additional, Verani, M., additional, Federigi, I., additional, Iaconelli, M., additional, Bonanno Ferraro, G., additional, Mancini, P., additional, Veneri, C., additional, Ferretti, E., additional, Lucentini, L., additional, Gramaccioni, L., additional, and La Rosa, G., additional

Published
2019
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33. Hepatitis E in Italy: 5 years of national epidemiological, virological and environmental surveillance, 2012 to 2016

Author

Alfonsi, V., Romano, L., Ciccaglione, A. R., La Rosa, G., Bruni, R., Zanetti, A., Libera, S. D., Iaconelli, M., Bagnarelli, P., Capobianchi, M. R., Garbuglia, A. R., Riccardo, F., Tosti, M. E., Ferrigno, L., Crateri, S., Galli, C., Tagliacarne, C., Giordani, M. T., Morelli, E., Vit, A., Bertin, T., Marinello, S., De Checchi, G., Zotti, C., Amprino, V., Rossati, A., Di Vito, A., Guidoni, C., Fiacchini, D., Marinelli, K., Loffredo, M., De Angelis, G., Citarella, A., and Megnia, A. S.

Subjects
0301 basic medicine, Swine, Sewage, Wastewater, medicine.disease_cause, Surveillance and Outbreak Report, 0302 clinical medicine, Hepatitis E virus, Epidemiology, Genotype, 80 and over, risk factors, 030212 general & internal medicine, Viral, Child, Phylogeny, Aged, 80 and over, Travel, Transmission (medicine), Middle Aged, Hepatitis E, 6. Clean water, 3. Good health, One Health, Italy, surveillance, RNA, Viral, epidemiology, Travel-Related Illness, Environmental Monitoring, Adult, medicine.medical_specialty, Adolescent, viral infections, Food Contamination, 03 medical and health sciences, Young Adult, Virology, Environmental health, medicine, Animals, Humans, Waste Water, Risk factor, Aged, business.industry, Public Health, Environmental and Occupational Health, medicine.disease, 030104 developmental biology, RNA, business
Abstract

Increasing numbers of hepatitis E cases are being reported in several European countries, including Italy, but the burden of hepatitis E virus (HEV) infection is largely unknown in the latter. To gain a better understanding of HEV epidemiology at national level in Italy, we piloted a strengthened and integrated human (epidemiological and virological) and environmental HEV surveillance system between 2012 and 2016. Over the 5-year period, 169 confirmed hepatitis E cases were identified, with a national annual incidence of 0.72 cases per 1,000,000. Of 65 HEV-RNA positive samples of sufficient quality for molecular analysis, 66% were genotype HEV3, 32% HEV1 and 1% HEV4. The most frequent risk factor reported by all HEV3 infected cases, was the consumption of undercooked pork and sausage. For the environmental surveillance, 679 urban sewage samples were collected from 53 wastewater treatment plants and HEV-RNA was detected in 38/679 of the samples. Among these, 25 (66%) were genotype HEV3 and the remaining were HEV1. We demonstrate that autochthonous transmission and environmental circulation of genotype HEV3 is adding to travel-related HEV transmission in Italy. We recommend the ‘One Health’ approach to integrated surveillance, and to include HEV-related messages within health information campaigns focussing on food security.

Published
2018

49. Molecular characterization of human Sapovirus in untreated sewage in Italy by amplicon‐based Sanger and next‐generation sequencing.

Author

Mancini, P., Bonanno Ferraro, G., Iaconelli, M., Suffredini, E., Valdazo‐González, B., Della Libera, S., Divizia, M., and La Rosa, G.

Subjects
MOLECULAR structure, GASTROENTERITIS, SEWAGE disposal plants, POLYMERASE chain reaction, NUCLEOTIDE sequencing
Abstract

Aims: Human Sapoviruses (HSaVs) are etiological agents of sporadic cases and outbreaks of acute gastroenteritis in humans of all ages. Evidence of worldwide distribution of HSaV has been documented; however, little is known about HSaV circulation in Italy. To study their occurrence and genetic diversity a nation‐wide environmental surveillance was undertaken. Methods and Results: One hundred and sixty‐six raw sewage samples, collected from 16 wastewater treatment plants throughout Italy, were processed and analysed by a RT‐nested PCR targeting the capsid region, followed by amplicon sequencing. HSaV was detected in 56 of 166 (33·7%) samples, characterized as genotypes GI.1 (n = 30 samples), GI.2 (n = 3), GI.3 (n = 2) and GII.1 (n = 1). Mixed electropherograms were detected in 20 samples. Next‐generation sequencing (NGS)‐based amplicon sequencing was performed on pools of PCR amplicons to detect viruses in mixed samples and less prevalent genotypes undetectable by conventional Sanger sequencing. NGS revealed three additional genotypes (GI.6, GII.6 and GV.1) beyond the four detected by Sanger sequencing. Conclusions: Our results show a significant circulation of HSaV in Italy with three genogroups (GI, GII and GV) and seven genotypes detected. The high detection rate in sewage samples suggests that HSaV infection in Italy could be underestimated or associated with asymptomatic or mild cases. Significance and Impact of the Study: The study detected HSaV in a relevant proportion of raw sewage samples, reflecting a considerable circulation of these viruses in the Italian population, pointing to the usefulness of including HSaV in testing patients with gastroenteritis. Furthermore, our results confirm that wastewater surveillance coupled with NGS is a powerful tool to study the molecular epidemiology of enteric viruses. [ABSTRACT FROM AUTHOR]

Published
2019
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