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2. P–461 A 16-year bicentric retrospective analysis of ovarian tissue cryopreservation (OTC) in paediatric patients: indications, results and outcome

Author

Grellet-Grün, M, primary, Delepine, B, additional, Va. Quyen, P L, additional, Durlach, A, additional, Greze, C, additional, Ladureau-Fritsch, L, additional, Lichtblau, I, additional, Canepa, A S, additional, Becmeur, F, additional, Liné, A, additional, Paillard, C, additional, Pluchart, C, additional, Pirello, O, additional, and Teletin, M, additional

Published
2021
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3. P–116 A 9-year monocentric retrospective analysis of glutaraldehyde-fixed and semithin section of testicular biopsies and TESE in azoospermic patients

Author

Cesbron, M, primary, Durand, J B, additional, Ladureau-Fritsch, L, additional, Greze, C, additional, Schmitt, F, additional, Pirrello, O, additional, Bettahar, K, additional, Ohl, J, additional, Rongieres, C, additional, Lichtblau, I, additional, Saussine, C, additional, Mark, M, additional, and Teletin, M, additional

Published
2021
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4. P-461 Pre-selected for an award: A 16-year bicentric retrospective analysis of ovarian tissue cryopreservation (OTC) in paediatric patients: indications, results and outcome

Author

Grellet-Grün, M, primary, Delepine, B, additional, Le Van Quyen, P, additional, Durlach, A, additional, Greze, C, additional, Ladureau-Fritsch, L, additional, Lichtblau, I, additional, Canepa, A S, additional, Becmeur, F, additional, Liné, A, additional, Paillard, C, additional, Pluchart, C, additional, Pirello, O, additional, and Teletin, M, additional

Published
2021
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7. Female (in)fertility

Author

Saad, H., primary, Khalil, E., additional, Bora, S. A., additional, Parikh, J., additional, Abdalla, H., additional, Thum, M. Y., additional, Bina, V., additional, Roopa, P., additional, Shyamala, S., additional, Anupama, A., additional, Tournaye, H., additional, Polyzos, N. P., additional, Guzman, L., additional, Nelson, S. M., additional, Lourenco, B., additional, Sousa, A. P., additional, Almeida-Santos, T., additional, Ramalho-Santos, J., additional, Okhowat, J., additional, Wirleitner, B., additional, Neyer, T., additional, Bach, M., additional, Murtinger, M., additional, Zech, N. H., additional, Nwoye, M., additional, Corona, R., additional, Blockeel, C., additional, Stoop, D., additional, Camus, M., additional, Rajikin, M. H., additional, Kamsani, Y. S., additional, Chatterjee, A., additional, Nor-Ashikin, M. N. K., additional, Nuraliza, A. S., additional, Scaravelli, G., additional, D'Aloja, P., additional, Bolli, S., additional, De Luca, R., additional, Spoletini, R., additional, Fiaccavento, S., additional, Speziale, L., additional, Vigiliano, V., additional, Farquhar, C., additional, Brown, J., additional, Arroll, N., additional, Gupta, D., additional, Boothroyd, C., additional, Al Bassam, M., additional, Moir, J., additional, Johnson, N., additional, Pantasri, T., additional, Robker, R. L., additional, Wu, L. L., additional, Norman, R. J., additional, Buzaglo, K., additional, Velez, M., additional, Shaulov, T., additional, Sylvestre, C., additional, Kadoch, I. J., additional, Krog, M., additional, Prior, M., additional, Carlsen, E., additional, Loft, A., additional, Pinborg, A., additional, Andersen, A. N., additional, Dolleman, M., additional, Verschuren, W. M. M., additional, Eijkemans, M. J. C., additional, Dolle, M. E. T., additional, Jansen, E. H. J. M., additional, Broekmans, F. J. M., additional, Van der Schouw, Y. T., additional, Fainaru, O., additional, Pencovich, N., additional, Hantisteanu, S., additional, Barzilay, I., additional, Ellenbogen, A., additional, Hallak, M., additional, Cavagna, M., additional, Baruffi, R. L. R., additional, Petersen, C. G., additional, Mauri, A. L., additional, Massaro, F. C., additional, Ricci, J., additional, Nascimento, A. M., additional, Vagnini, L. D., additional, Pontes, A., additional, Oliveira, J. B. A., additional, Franco, J. G., additional, Canas, M. C. T., additional, Nicoletti, A., additional, Martins, A. M. V. C., additional, Lichtblau, I., additional, Olivennes, F., additional, Aubriot, F. A., additional, Junca, A. M., additional, Belloc, S., additional, Cohen-Bacrie, M., additional, Cohen-Bacrie, P., additional, de Mouzon, J., additional, Nandy, T., additional, Caragia, A., additional, Balestrini, S., additional, Zosmer, A., additional, Sabatini, L., additional, Al-Shawaf, T., additional, Seshadri, S., additional, Khalaf, Y., additional, Sunkara, S. K., additional, Joy, J., additional, Lambe, M., additional, Lutton, D., additional, Nicopoullos, J., additional, Faris, R., additional, Behre, H. M., additional, Howles, C. M., additional, Longobardi, S., additional, Chimote, N., additional, Mehta, B., additional, Nath, N., additional, Chimote, N. M., additional, Mine, K., additional, Yoshida, A., additional, Yonezawa, M., additional, Ono, S., additional, Abe, T., additional, Ichikawa, T., additional, Tomiyama, R., additional, Nishi, Y., additional, Kuwabara, Y., additional, Akira, S., additional, Takeshita, T., additional, Shin, H., additional, Song, H. S., additional, Lim, H. J., additional, Hauzman, E., additional, Kohls, G., additional, Barrio, A., additional, Martinez-Salazar, J., additional, Iglesias, C., additional, Velasco, J. A. G., additional, Tejada, M. I., additional, Maortua, H., additional, Mendoza, R., additional, Prieto, B., additional, Martinez-Bouzas, C., additional, Diez-Zapirain, M., additional, Martinez-Zilloniz, N., additional, Matorras, R., additional, Amaro, A., additional, Bianco, B., additional, Christofolini, J., additional, Mafra, F. A., additional, Barbosa, C. P., additional, Christofolini, D. M., additional, Pesce, R., additional, Gogorza, S., additional, Ochoa, C., additional, Gil, S., additional, Saavedra, A., additional, Ciarmatori, S., additional, Perman, G., additional, Pagliardini, L., additional, Papaleo, E., additional, Corti, L., additional, Vanni, V. S., additional, Ottolina, J., additional, de Michele, F., additional, Marca, A. L., additional, Vigano, P., additional, Candiani, M., additional, Li, L., additional, Yin, Q., additional, Huang, L., additional, Huang, J., additional, He, Z., additional, Yang, D., additional, Tiplady, S., additional, Ledger, W., additional, Godbert, S., additional, Hart, S., additional, Johnson, S., additional, Wong, A. W. Y., additional, Kong, G. W. S., additional, Haines, C. J., additional, Franik, S., additional, Nelen, W., additional, Kremer, J., additional, Gillett, W. R., additional, Lamont, J. M., additional, Peek, J. C., additional, Herbison, G. P., additional, Sung, N. Y., additional, Hwang, Y. I., additional, Choi, M. H., additional, Song, I. O., additional, Kang, I. S., additional, Koong, M. K., additional, Lee, J. S., additional, Yang, K. M., additional, Celtemen, M. B., additional, Telli, P., additional, Karakaya, C., additional, Bozkurt, N., additional, Gursoy, R. H., additional, Younis, J. S., additional, Ben-Ami, M., additional, Pundir, J., additional, Pundir, V., additional, Omanwa, K., additional, and El-Toukhy, T., additional

Published
2013
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12. FEMALE (IN)FERTILITY

Author

Kanta Goswami, S., primary, Banerjee, S., additional, Saha, P., additional, Chakraborty, P., additional, Kabir, S. N., additional, Karimzadeh, M. A., additional, Mohammadian, F., additional, Mashayekhy, M., additional, Saldeen, P., additional, Kallen, K., additional, Karlstrom, P. O., additional, Rodrigues-Wallberg, K. A., additional, Salerno, A., additional, Nazzaro, A., additional, Di Iorio, L., additional, Marino, S., additional, Granato, C., additional, Landino, G., additional, Pastore, E., additional, Ghoshdastidar, B., additional, Chakraborty, C., additional, Ghoshdastidar, B. N., additional, Ghoshdastidar, S., additional, Partsinevelos, G. A., additional, Papamentzelopoulou, M., additional, Mavrogianni, D., additional, Marinopoulos, S., additional, Dinopoulou, V., additional, Theofanakis, C., additional, Anagnostou, E., additional, Loutradis, D., additional, Franz, C., additional, Nieuwland, R., additional, Montag, M., additional, Boing, A., additional, Rosner, S., additional, Germeyer, A., additional, Strowitzki, T., additional, Toth, B., additional, Mohamed, M., additional, Vlismas, A., additional, Sabatini, L., additional, Caragia, A., additional, Collins, B., additional, Leach, A., additional, Zosmer, A., additional, Al-Shawaf, T., additional, Beyhan, Z., additional, Fisch, J. D., additional, Danner, C., additional, Keskintepe, L., additional, Aydin, Y., additional, Ayca, P., additional, Oge, T., additional, Hassa, H., additional, Papanikolaou, E., additional, Pados, G., additional, Grimbizis, G., additional, Bili, H., additional, Karastefanou, K., additional, Fatemi, H., additional, Kyrou, D., additional, Humaidan, P., additional, Tarlatzis, B., additional, Gungor, F., additional, Karamustafaoglu, B., additional, Iyibozkurt, A. C., additional, Ozsurmeli, M., additional, Bastu, E., additional, Buyru, F., additional, Di Emidio, G., additional, Vitti, M., additional, Mancini, A., additional, Baldassarra, T., additional, D'Alessandro, A. M., additional, Polsinelli, F., additional, Tatone, C., additional, Leperlier, F., additional, Lammers, J., additional, Dessolle, L., additional, Lattes, S., additional, Barriere, P., additional, Freour, T., additional, Elodie, P., additional, Assou, S., additional, Van den Abbeel, E., additional, Arce, J. C., additional, Hamamah, S., additional, Dechaud, H., additional, Haouzi, D., additional, Tiplady, S., additional, Johnson, S., additional, Jones, G., additional, Ledger, W., additional, Eizadyar, N., additional, Ahmad Nia, S., additional, Seyed Mirzaie, M., additional, Azin, S. A., additional, Yazdani Safa, M., additional, Onaran, Y., additional, Iltemir Duvan, C., additional, Keskin, E., additional, Ayrim, A., additional, Kafali, H., additional, Kadioglu, N., additional, Guler, B., additional, Var, T., additional, Cicek, M. N., additional, Batioglu, A. S., additional, Lichtblau, I., additional, Olivennes, F., additional, de Mouzon, J., additional, Dumont, M., additional, Junca, A. M., additional, Cohen-Bacrie, M., additional, Hazout, A., additional, Belloc, S., additional, Cohen-Bacrie, P., additional, Allegra, A., additional, Marino, A., additional, Sammartano, F., additional, Coffaro, F., additional, Scaglione, P., additional, Gullo, S., additional, Volpes, A., additional, Prisant, N., additional, Saare, M., additional, Vaidla, K., additional, Salumets, A., additional, Peters, M., additional, Jindal, U. N., additional, Thakur, M., additional, Shvell, V., additional, Diamond, M. P., additional, Awonuga, A. O., additional, Veljkovic, M., additional, Macanovic, B., additional, Milacic, I., additional, Borogovac, D., additional, Arsic, B., additional, Pavlovic, D., additional, Lekic, D., additional, Bojovic Jovic, D., additional, Garalejic, E., additional, Jayaprakasan, K., additional, Eljabu, H., additional, Hopkisson, J., additional, Campbell, B., additional, Raine-Fenning, N., additional, Kop, P., additional, van Wely, M., additional, Mol, B. W., additional, Melker, A. A., additional, Janssens, P. M. W., additional, Nap, A., additional, Arends, B., additional, Roovers, J. P. W. R., additional, Ruis, H., additional, Repping, S., additional, van der Veen, F., additional, Mochtar, M. H., additional, Sargin, A., additional, Yilmaz, N., additional, Gulerman, C., additional, Guven, A., additional, Polat, B., additional, Ozel, M., additional, Bardakci, Y., additional, Vidal, C., additional, Giles, J., additional, Remohi, J., additional, Pellicer, A., additional, Garrido, N., additional, Javdani, M., additional, Fallahzadeh, H., additional, Davar, R., additional, Sheibani, H., additional, Leary, C., additional, Killick, S., additional, Sturmey, R. G., additional, Kim, S. G., additional, Lee, K. H., additional, Park, I. H., additional, Sun, H. G., additional, Lee, J. H., additional, Kim, Y. Y., additional, Choi, E. M., additional, Van Loendersloot, L. L., additional, Van Wely, M., additional, Bossuyt, P. M. M., additional, Van Der Veen, F., additional, Roychoudhury Sarkar, M., additional, Roy, D., additional, Sahu, R., additional, Bhattacharya, J., additional, Eguiluz Gutierrez- Barquin, I., additional, Sanchez Sanchez, V., additional, Torres Afonso, A., additional, Alvarez Sanchez, M., additional, De Leon Socorro, S., additional, Molina Cabrillana, J., additional, Seara Fernandez, S., additional, Garcia Hernandez, J. A., additional, Ozkan, Z. S., additional, Simsek, M., additional, Kumbak, B., additional, Atilgan, R., additional, Sapmaz, E., additional, Agirregoikoa, J. A., additional, DePablo, J. L., additional, Abanto, E., additional, Gonzalez, M., additional, Anarte, C., additional, Barrenetxea, G., additional, Aleyasin, A., additional, Mahdavi, A., additional, Agha Hosseini, M., additional, Safdarian, L., additional, Fallahi, P., additional, Bahmaee, F., additional, Sarikaya, E., additional, Segawa, T., additional, Teramoto, S., additional, Tsuchiyama, S., additional, Miyauchi, O., additional, Watanabe, Y., additional, Ohkubo, T., additional, Shozu, M., additional, Ishikawa, H., additional, Yelian, F., additional, Papaioannou, S., additional, Knowles, T., additional, Aslam, M., additional, Milnes, R., additional, Takashima, A., additional, Takeshita, N., additional, Kinoshita, T., additional, Chapman, M. G., additional, Kilani, S., additional, Dadras, N., additional, Parsanezhad, M. E., additional, Zolghadri, J., additional, Younesi, M., additional, Floehr, J., additional, Dietzel, E., additional, Wessling, J., additional, Neulen, J., additional, Rosing, B., additional, Tan, S., additional, Jahnen-Dechent, W., additional, Lee, K. S., additional, Joo, J. K., additional, Son, J. B., additional, Joo, B. S., additional, Risquez, F., additional, Confino, E., additional, Llavaneras, F., additional, Marval, I., additional, D'Ommar, G., additional, Gil, M., additional, Risquez, M., additional, Lozano, L., additional, Paublini, A., additional, Piras, M., additional, Risquez, A., additional, Prochazka, R., additional, Blaha, M., additional, Nemcova, L., additional, Weghofer, A., additional, Kim, A., additional, Barad, D. H., additional, Gleicher, N., additional, Kilic, Y., additional, Ergun, B., additional, Howard, B., additional, Weiss, H., additional, Doody, K., additional, Schafer, C., additional, Ensslen, S., additional, Denecke, B., additional, Veitinger, T., additional, Spehr, M., additional, Tropartz, T., additional, Tolba, R., additional, Egert, A., additional, Schorle, H., additional, Alanya, S., additional, and Yumru, H., additional

Published
2012
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13. SESSION 44: TREATMENT OUTCOMES

Author

Setti, A. S., primary, Braga, D. P. A. F., additional, Figueira, R. C. S., additional, Aoki, T., additional, Iaconelli, A., additional, Borges, E., additional, Caanen, M., additional, Kuijper, E., additional, Homburg, R., additional, Hompes, P., additional, Kushnir, M., additional, Lambalk, C. B., additional, Monahan, D., additional, Neri, Q., additional, Kocent, J., additional, Schlegel, P. N., additional, Rosenwaks, Z., additional, Palermo, G. D., additional, Belloc, S., additional, de Mouzon, J., additional, Cohen-Bacrie, M., additional, Lichtblau, I., additional, Dumont, M., additional, Junca, A. M., additional, Hazout, A., additional, Cohen-Bacrie, P., additional, Bensdorp, A. J., additional, Hukkelhoven, C. P. W. M., additional, Mol, B. W., additional, and van Wely, M., additional

Published
2012
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16. COVID-19 and Oncofertility: No SARS-CoV-2 in Semen but Inflammation Seems to Affect Sperm Parameters.

Author

Chaput L, Pons-Rejraji H, Chabrolles H, Fiot M, Lucas C, Pereira B, Bonnet B, Haj Hamid R, Rives-Feraille A, Binois O, Ferreux L, Delepine B, Koscinski I, Lichtblau I, Giscard d'Estaing S, Bendayan M, Saias-Magnan J, Lousqui J, Henquell C, and Brugnon F

Subjects
Humans, Male, Adult, Prospective Studies, Middle Aged, Inflammation virology, Cytokines analysis, Fertility Preservation methods, Cryopreservation, Semen Analysis, Tumor Necrosis Factor-alpha, COVID-19 immunology, Semen virology, Spermatozoa virology, SARS-CoV-2 immunology, Neoplasms
Abstract

The COVID-19 pandemic, driven by SARS-CoV-2, led authorities to recommend halting assisted reproductive technology programs, focusing instead on fertility preservation, for cancer patients. The presence of SARS-CoV-2 in semen remains controversial. This multicentric prospective cohort study, conducted across 12 university medical centers, aimed to determine if SARS-CoV-2 is present in spermatozoa/seminal plasma in cancer patients by RT-PCR and to assess its impact on standard semen parameters. The levels of cytokines and TNF-α were measured in seminal fluid by ELISA. We enrolled 129 men who underwent sperm cryopreservation between July 7, 2020, and June 30, 2021. The 63 were included and tested for COVID-19 in nasal swab samples by RT-PCR and/or by serology. All patients were asymptomatic on the day of semen collection: 50 were uninfected, 8 had a positive nasal swab (PCR+) and 5 were seropositive. SARS-CoV-2 RNA was not detected in the seminal fluid or spermatozoa. Ejaculate volume was significantly lower in the PCR+ group compared to the uninfected group (median [IQR]: 2.6 mL [1.6-3.4] vs. 4.6 mL [2.6-5.2] p < 0.05). Total and progressive motility were lower in the PCR+ group compared to the seropositive group (32.5% [25.0-45.0] vs. 50% [49.0-55.0] p < 0.05, and 22.5% [10.0; 32.5] vs. 44.5% [40-49] p < 0.05). Higher TNF-α level was observed in the PCR+ group (1.9 pg/mL [0-3.9]) compared to the uninfected group (0 pg/mL [0-0.4]) p < 0.05. Although SARS-CoV-2 was not detected in the sperm samples of cancer patients who were PCR+, the infection appears to impact sperm parameters, likely due to inflammation., (© 2024 The Author(s). Journal of Medical Virology published by Wiley Periodicals LLC.)

Published
2024
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17. A 16-year bicentric retrospective analysis of ovarian tissue cryopreservation in pediatric units: indications, results, and outcome.

Author

Grellet-Grün M, Delepine B, Le Van Quyen P, Avérous G, Durlach A, Greze C, Ladureau-Fritsch L, Lichtblau I, Canepa AS, Liné A, Paillard C, Pluchart C, Pirrello O, Rongieres C, Harika G, Becmeur F, and Teletin M

Subjects
Adolescent, Adult, Female, Humans, Child, Retrospective Studies, Cryopreservation, Fertility Preservation, Menopause, Premature, Primary Ovarian Insufficiency
Abstract

Background: Cancer treatments of the last decades improve the survival rate of children and adolescents. However, chemo- and radiotherapy result in gonadal damage, leading to acute ovarian failure and sterility. The preservation of fertility is now an integral part of care of children requiring gonadotoxic treatments. Ovarian tissue cryopreservation (OTC) is an effective fertility preservation option that allows long-term storage of primordial follicles, subsequent transplantation, and restoration of endocrine function and fertility. The efficacy of this technique is well-demonstrated in adults but the data are scarce for pediatric patients. Currently, OTC represents the only possibility of preserving the potential fertility in prepubertal girls., Procedure: This is a retrospective study of OTC practice of two French centers from January 2004 to May 2020. A total of 72 patients from pediatric units underwent cryopreservation of ovarian tissue before gonadotoxic therapy for malignant or non-malignant diseases. The ovarian cortex was cut into fragments and the number of follicles per square millimeter was evaluated histologically. The long-term follow-up includes survival rate and hormonal and fertility status., Results: The mean age of patients at OTC was 9.3 years [0.2-17] and 29.2% were postpubertal; 51 had malignant diseases and 21 had non-malignant diseases. The most frequent diagnoses included acute leukemia, hemoglobinopathies, and neuroblastoma. Indication for OTC was stem cell transplantation for 81.9% ( n  = 59) of the patients. A third of each ovary was collected for 62.5% ( n  = 45) of the patients, a whole ovary for 33.3% ( n  = 24) of the patients, and a third of one ovary for 4.2% ( n  = 3) of the patients. An average of 17 fragments [5-35] per patient was cryoconserved. A correlation was found between the age of the patients and the number of fragments ( p  < 0.001). More fragments were obtained from partial bilateral harvesting than from whole ovary harvesting ( p  < 0.05). Histological analysis of ovarian tissue showed a median of 6.0 primordial follicles/mm 2 [0.0-106.5] and no malignant cells were identified. A negative correlation was found between age and follicular density ( p  < 0.001). Median post-harvest follow-up was 92 months [1-188]. A total of 15 girls had died, 11 were still under treatment for their pathology, and 46 were in complete remission. Of all patients, 29 (40.2%) were subjected to a hormonal status evaluation and 26 were diagnosed with premature ovarian insufficiency (POI) ( p  < 0.001). One patient had undergone thawed ovarian tissue transplantation., Conclusion: OTC should be proposed to all girls with high risk of developing POI following gonadotoxic therapies in order to give them the possibility of fertility and endocrine restoration., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2023 Grellet-Grün, Delepine, Le Van Quyen, Avérous, Durlach, Greze, Ladureau-Fritsch, Lichtblau, Canepa, Liné, Paillard, Pluchart, Pirrello, Rongieres, Harika, Becmeur and Teletin.)

Published
2023
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18. Luteal phase stimulation, the future of fertility preservation? Retrospective cohort study of luteal phase versus follicular phase stimulation.

Author

Jochum F, Sananès N, Teletin M, Lichtblau I, Rongières C, and Pirrello O

Subjects
Adult, Cohort Studies, Female, France, Humans, Neoplasms therapy, Oocytes physiology, Retrospective Studies, Fertility Preservation methods, Follicular Phase physiology, Luteal Phase physiology, Ovulation Induction methods
Abstract

Research Question: Is luteal phase stimulation capable of improving fertility preservation?, Materials and Methods: We performed a retrospective cohort study of consecutive ovarian stimulations, during July 2012 and September 2018 at Strasbourg University Teaching Hospital in France. Enrollment criteria were patients aged below 40 who had been referred to our center following a diagnosis of cancer or requiring gonadotoxic treatment. All patients enrolled had regular menstrual cycles and normal ovulation. Non-enrollment criteria were an expected low ovarian response (defined by an anti-Müllerian hormone (AMH) level <0.75μg/L and/or an antral follicle count inferior (AFC) inferior than 5), polycystic ovarian syndrome, amenorrhea, prior history of infertility or gonadotoxic treatment. The primary endpoint is the number of mature oocytes (metaphase II) obtained. Secondary outcomes were oocyte yields obtained, stimulation duration, initial gonadotropin dose and total gonadotropin dose., Results: A total of 100 patients were included: 20 in luteal phase and 80 in follicular phase. A larger number of mature oocytes was obtained in luteal phase versus follicular phase (13.1+/8.0 versus 9.2+/-5.8 with p=0.01). A greater amount of total (mature and immature) oocytes was obtained in luteal phase versus follicular phase with a significant difference (16.8+/-9.3 versus 11.8+/-7.3 with p=0.01). No difference was found for the initial and total doses of gonadotropin., Conclusions: Luteal phase stimulation has the advantage of a better flexibility with positives results as to the number of oocytes obtained in fertility preservation., (Copyright © 2018 Elsevier Masson SAS. All rights reserved.)

Published
2019
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19. Luteal phase progesterone supplementation following induced natural cycle frozen embryo transfer: A retrospective cohort study.

Author

Schwartz E, Bernard L, Ohl J, Bettahar K, Rongières C, Lichtblau I, and Pirrello O

Subjects
Administration, Intravaginal, Adult, Cohort Studies, Cryopreservation, Embryo, Mammalian physiology, Female, Fertilization in Vitro, Humans, Ovulation Induction, Pregnancy, Pregnancy Rate, Retrospective Studies, Sperm Injections, Intracytoplasmic, Embryo Transfer methods, Luteal Phase, Progesterone administration & dosage
Abstract

Introduction: The objective of this study was to assess the impact on the clinical pregnancy rate of luteal phase progesterone treatment in patients being prepared for natural cycle frozen embryo transfer (FET) with induced ovulation., Material and Methods: This retrospective cohort study collect all the FET protocols over a 6-month period at Strasbourg University Hospital fertility unit between December 2016 and May 2017. In total 293 consecutive patients with regular menstrual cycles were prepared for natural cycle FET during this period. All patients had an embryo cryopreservation secondary to in vitro fertilisation (IVF) or by intracytoplasmic sperm injection (ICSI). There were 2 protocols during this period and patients either received or did not received progesterone. Ovulation was routinely triggered in all patients by injection of choriogonadotrophin alfa. Patients in the treated group received vaginal natural micronized progesterone treatment of 400mg daily, starting on the day of ovulation. The principal assessment criterion was the occurrence of pregnancy., Results: In total, 231 patients were analysed: 108 in the group not receiving progesterone and 123 in the group receiving progesterone. Patient characteristics were comparable between groups. A higher clinical pregnancy rate (39% vs. 24.1%, p=0.02; 95CI [1.10; 3.74]) was recorded in the treated group., Conclusions: Our results suggest that luteal phase support with vaginal progesterone statistically increases the clinical pregnancy rate following hCG-triggered natural cycle FET and that it should be used more widely., (Copyright © 2018 Elsevier Masson SAS. All rights reserved.)

Published
2019
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20. Oxidative stress and fertility: incorrect assumptions and ineffective solutions?

Author

Ménézo Y, Entezami F, Lichtblau I, Belloc S, Cohen M, and Dale B

Subjects
DNA Damage drug effects, Fertility drug effects, Humans, Male, Antioxidants pharmacology, Fertility physiology, Oxidative Stress, Reactive Oxygen Species metabolism, Reproduction drug effects
Abstract

One of the most important concerns in assisted reproduction (ART), and in particular ICSI, is the quality of sperm DNA. Oxidative stress is one of the major causes of damage to DNA and attempting to reduce generation of DNA damage related to reactive oxygen species (ROS) through consumption of antioxidants is often tempting. However, current antioxidant treatments, given irrespectively of clinically quantified deficiencies, are poorly efficient, potentially detrimental and over-exposure is risky. Here we discuss new treatments in relation to present day concepts on oxidative stress. This discussion includes stimulation of endogenous anti-ROS defense i.e. glutathione synthesis and recycling of homocysteine, the epicentre of multiple ROS-linked pathologies.

Published
2014
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21. New insights into human pre-implantation metabolism in vivo and in vitro.

Author

Ménézo Y, Lichtblau I, and Elder K

Subjects
Amino Acids metabolism, Genomic Imprinting physiology, Humans, Lipid Metabolism physiology, Oxidative Stress physiology, Vitamins metabolism, Embryonic Development physiology, Fertilization in Vitro, Genomic Imprinting genetics, Metabolism genetics, Metabolism physiology
Abstract

The metabolism of pre-implantation embryos is far from being understood. In human embryos, the two major obstacles are the scarcity of material, for obvious ethical reasons, and complete absence of a relevant in vivo control model. Over-extrapolation from animal species to human systems adds to the complexity of the problem. Removal of some metabolites from media has been proposed, such as glucose and essential amino acids, on the basis of their pseudo "toxicity". In contrast, addition of some compounds such as growth factors has been proposed in order to decrease apoptosis, which is a natural physiologic process. These suggestions reflect the absence of global knowledge, and in consequence mask reality. Some aspects of metabolism have been ignored, such as lipid metabolism. Others are seriously underestimated, such as oxidative stress and its relationship to imprinting/methylation, of paramount importance for genetic regulation and chromosomal stability. It has become increasingly obvious that more studies are essential, especially in view of the major extension of ART activities worldwide.

Published
2013
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22. [Oxidative stress and fertility: false evidence and bad recipes].

Author

Ménézo Y, Entezami F, Lichtblau I, Cohen M, Belloc S, and Brack M

Subjects
Antioxidants administration & dosage, Antioxidants adverse effects, Ascorbic Acid administration & dosage, DNA Damage, DNA Repair, Dietary Supplements, Female, Humans, Infertility, Female etiology, Infertility, Male etiology, Male, Oocytes physiology, Selenium administration & dosage, Spermatozoa physiology, Superoxide Dismutase, Ubiquinone administration & dosage, Ubiquinone analogs & derivatives, Infertility etiology, Oxidative Stress
Abstract

Worldwide statistics agree that at least one out of six couples has fertility problems. If the male gamete is the origin of this problem, it is generally admitted that the oxidative stress is involved. Modern life has obviously increased fertility problems through pesticides, xenoestrogenes, endocrine disrupting chemicals involved in plastic technology such as polychlorinated bisphenyls, bisphenol A, phthalates and alkylphenols… and other cosmetic additives. An important part of these compounds increases oxidative stress, at least in part. Oxidative stress is more than probably at the origin or recurrent increasing pathologies such as endometriosis. If the oocyte is theoretically able to repair oxidative stress linked decays such as DNA fragmentation and oxidation of bases, its capacity is finite and decreasing with age. In order to decrease DNA repair charge, reducing or even avoiding the generation of DNA damages related to reactive oxygen species through consumption of antioxidants compounds is often tempting: however Reasons will be provided to break from current treatments given haphazardly in the population in the age of reproduction, as well as the potential risks of over-exposure. Furthermore recommended treatments, in relation with the new concepts in oxidative stress, will be specified., (Copyright © 2012 Elsevier Masson SAS. All rights reserved.)

Published
2012
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23. Carnitine content in the follicular fluid and expression of the enzymes involved in beta oxidation in oocytes and cumulus cells.

Author

Montjean D, Entezami F, Lichtblau I, Belloc S, Gurgan T, and Menezo Y

Subjects
Adolescent, Adult, Cumulus Cells metabolism, Female, Fertilization in Vitro methods, Humans, Lipid Metabolism, Oocytes metabolism, Oxidation-Reduction, Pregnancy, Young Adult, Carnitine metabolism, Cumulus Cells enzymology, Follicular Fluid metabolism, Mixed Function Oxygenases metabolism, Oocytes enzymology, gamma-Butyrobetaine Dioxygenase metabolism
Abstract

Purpose: The purpose of this study is to study lipid metabolism in oocytes and embryos that is a neglected parameter in human IVF., Methods: We have tested the total carnitine content (TC) in the follicular fluid of 278 patients (217 non pregnant, 61 pregnant) undergoing IVF., Results: The follicular fluid TC is neither correlated with the circulating estradiol content in serum nor with the outcome the IVF attempt. Carnitine, through the carnitine shuttle, is a major partner in lipid beta oxidation, metabolic pathway involved in the acquisition of oocyte competence. The expression of carnitine synthesis enzymes and lipid beta oxidation was studied in cumulus cells collected at the time of ovum retrieval and in oocyte. Surprisingly the expression for carnitine synthesis is not detectable in oocytes whereas the enzymes involved in lipid beta oxidation are rather strongly expressed., Conclusions: The addition of carnitine in oocyte maturation and embryo culture media should not be overlooked.

Published
2012
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24. [Female fertility preservation before sterilizing treatment: contribution of ovarian tissue cryopreservation].

Author

Poirot C, Martelli H, Lichtblau I, Dhedin N, Abirached F, Faraguet C, and Vacher-Lavenu MC

Subjects
Female, Fertilization in Vitro, Humans, Neoplasms therapy, Cryopreservation methods, Embryo, Mammalian, Fertility, Oocytes, Ovary
Abstract

Longer survival after anticancer treatment has lead to concern about the long-term adverse effects. Altered fertility is of particular importance. Before sterilizing treatment, three non-exclusive methods can be proposed to preserve female fertility: in vitro fertilization followed by cryopreservation of embryos, cryopreservation of mature ovocytes, cryopreservation of ovarian tissue. The method or methods chosen will depend on the age of the patient, here marital status, the urgency of the treatment, and the type of disease. Embryo cryopreservation is a routine practice in medically assisted reproduction centers, while cryopreservation of mature ovocytes and ovarian tissue is still in the experimental phase. It is known however that mature ovocytes can be used after cryopreservation. Cyropreservation of ovarian tissue is a more difficult problem. To date, there have not been any pregnancies or births after freezing-thawing of human ovarian tissue. This tissue could be used in two ways: autograft and in vitro folliculo-ovocyte maturation. Despite the uncertainty concerning use, women cryopreservation of ovarian tissue quite well.

Published
2002

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