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4. Phosphoglucoisomerase-catalyzed interconversion of hexose phosphates: Study by 13C NMR of proton and deuteron exchange

Author

Malaisse, Willy, Liemans, Veronique, Malaisse Lagae, Francine, Ottinger, Robert, Willem, Rudolph, Malaisse, Willy, Liemans, Veronique, Malaisse Lagae, Francine, Ottinger, Robert, and Willem, Rudolph

Abstract

The exchange of protons and deuterons by phosphoglucoisomerase during the single passage conversion of D-[2-13C,1-2H]fructose 6-phosphate in H2O or D-[2-13C]fructose 6-phosphate in D2O to D-[2-13C]glucose 6-phosphate, as coupled with the further generation of 6-phospho-D-[2-13C]gluconate in the presence of excess glucose-6-phosphate dehydrogenase was investigated by 13C NMR spectroscopy of the latter metabolite. In H2O, the intramolecular deuteron transfer from the C1 of D-fructose 6-phosphate to the C2 of D-glucose 6-phosphate amounted to 65%, a value only slightly lower than the 72% intramolecular proton transfer in D2O. Both percentages, especially the latter one, were lower than those previously recorded during the single passage conversion of D-[1-13C,2-2H]glucose 6-phosphate in H2O or D-[1-13C]glucose 6-phosphate in D2O to D-fructose 6-phosphate and then to D-fructose 1,6-bisphosphate. These differences indicate that the sequence of interactions between the hexose esters and the binding sites of phosphoglucoisomerase is not strictly in mirror image during, respectively, the conversion of the aldose phosphate to ketose phosphate and the opposite process. © 1991 Kluwer Academic Publishers., SCOPUS: ar.j, info:eu-repo/semantics/published

Published
1991

9. Phosphoglucoisomerase-catalyzed interconversion of hexose phosphates: A model for D-[2-3H]glucose metabolism in human erythrocytes

Author

Liemans, Veronique, Malaisse, Willy, Liemans, Veronique, and Malaisse, Willy

Abstract

When D-[2-3H]glucose 6-phosphate mixed with the unlabeled ester is converted to D-[1-3H]fructose 6-phosphate and 3HOH in the phosphoglucoisomerase reaction and then to D-[1·3H]fructose 1,6- bisphosphate in the phosphofructokinase reaction, the specific radioactivity of the latter metabolite and the production of 3HOH relative to the total generation of tritiated end products are both inversely related to the concentration of phosphofructokinase. In human erythrocytes, the modeling of D-[2-3H]glucose metabolism, based on the activity of phosphoglucoisomerase in cell homogenates and on the steady-state content of D-glucose 6-phosphate and D-fructose 6-phosphate in intact cells, indicates that the back-and-forth interconversion of these esters is about five-times higher than the net glycolytic flux. Yet, the production of 3HOH from D-[2-3H]glucose is about 20% lower than the net glycolytic flux, as judged from the production of 3HOH from D-[5-3H]glucose. Thus, an incomplete detriation of D-[2- 3H]glucose is not incompatible with an extensive interconversion of hexose 6-phosphates in the reaction catalyzed by phosphoglucoisomerase., SCOPUS: ar.j, info:eu-repo/semantics/published

Published
1991

16. Non-enzymatic glycation of phosphoglucoisomerase

Author

Zahner, Dagmar, Liemans, Veronique, Malaisse Lagae, Francine, Malaisse, Willy, Zahner, Dagmar, Liemans, Veronique, Malaisse Lagae, Francine, and Malaisse, Willy

Abstract

info:eu-repo/semantics/published

Published
1989

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