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Your search keyword '"Lilyanna, Shera"' showing total 19 results
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19 results on '"Lilyanna, Shera"'

3. Finding a reliable assay for soluble neprilysin

Author

Liew, Oi Wah, Prickett, Timothy C.R., Revuelta-López, Elena, Ling, Samantha S.M., Cserkóová, Adriana, Lupón, Josep, Ng, Jessica Y.X., Chong, Jenny P.C., Lilyanna, Shera, Chan, Siew-Pang, Lin, Qifeng, Lim, Teck K., Lin, Qingsong, Barallat, Jaume, Bayés-Genís, Antoni, and Richards, A. Mark

Published
2022
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8. Natriuretic peptide receptor 3 (NPR3) is regulated by microRNA-100

Author

Wong, Lee Lee, Wee, Abby S.Y., Lim, Jia Yuen, Ng, Jessica Y.X., Chong, Jenny P.C., Liew, Oi Wah, Lilyanna, Shera, Martinez, Eliana C., Ackers-Johnson, Matthew Andrew, Vardy, Leah A., Armugam, Arunmozhiarasi, Jeyaseelan, Kandiah, Ng, Tze P., Lam, Carolyn S.P., Foo, Roger S.Y., Richards, Arthur Mark, and Chen, Yei-Tsung

Published
2015
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9. Immunoassay-Compatible Inactivation of SARS-CoV-2 in Plasma Samples for Enhanced Handling Safety

Author

Liew, Oi Wah, primary, Fanusi, Felic, additional, Ng, Jessica Yan Xia, additional, Ahidjo, Bintou Ahmadou, additional, Ling, Samantha Shi Min, additional, Lilyanna, Shera, additional, Chong, Jenny Pek Ching, additional, Lim, Angeline Eng Siew, additional, Lim, Wei Zheng, additional, Ravindran, Sindhu, additional, Chu, Justin Jang Hann, additional, Lim, Shir Lynn, additional, and Richards, Arthur Mark, additional

Published
2022
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10. Variability in Microplate Surface Properties and Its Impact on ELISA

Author

Lilyanna, Shera, primary, Ng, Enoch Ming Wei, additional, Moriguchi, Shiho, additional, Chan, Siew Pang, additional, Kokawa, Ryohei, additional, Huynh, So Hung, additional, Chong, P C Jenny, additional, Ng, Yan Xia, additional, Richards, A Mark, additional, Ng, Tuck Wah, additional, and Liew, Oi Wah, additional

Published
2018
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14. Grafts Enriched with Subamnion-Cord-Lining Mesenchymal Stem Cell Angiogenic Spheroids Induce Post-Ischemic Myocardial Revascularization and Preserve Cardiac Function in Failing Rat Hearts

Author

Martinez, Eliana C., primary, Vu, Duc-Thang, additional, Wang, Jing, additional, Lilyanna, Shera, additional, Ling, Lieng H., additional, Gan, Shu U., additional, Tan, Ai Li, additional, Phan, Thang T., additional, Lee, Chuen N., additional, and Kofidis, Theo, additional

Published
2013
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18. Abstract 107.

Author

Martinez, Eliana C, Lilyanna, Shera, Vardy, Leah A, Armugam, Arunmozhiarasi, Jeyaseelan, Kandiah, and Richards, Arthur M

Published
2014

19. One-Shot Generation of Epitope-Directed Monoclonal Antibodies to Multiple Nonoverlapping Targets: Peptide Selection, Antigen Preparation, and Epitope Mapping.

Author

Liew OW, Ling SSM, Lilyanna S, Chong JPC, Ng JYX, and Richards AM

Subjects
Amino Acid Sequence, Animals, Antigens, Epitope Mapping methods, Epitopes, Peptides genetics, Thioredoxins genetics, Antibodies, Monoclonal genetics, Peptide Library
Abstract

This chapter describes an epitope-directed approach to generate antipeptide monoclonal antibodies to multiple nonoverlapping protein sites using a cocktail of fusion peptides as immunogen. It provides a step-by-step protocol on how antigenic peptides on a target protein can be identified by in silico prediction and discusses considerations for final peptide selection. Each antigenic peptide (10-20 amino acids long) is displayed as three-copy inserts on the surface exposed loop of a thioredoxin scaffold protein. The corresponding DNA coding sequence specifying the tripeptide insert flanked by Gly-Ser-Gly-Ser-Gly linkers is cloned in-frame into the Rsr II site of the thioredoxin gene in the pET-32a vector. The presence of a C-terminal polyhistidine tag (His 6 -tag) allows the soluble fusion proteins to be purified by one-step native immobilized metal affinity chromatography (IMAC) to greater than 95% purity. Multiple thioredoxin fusion proteins are mixed in equimolar concentrations and used as an immunogen cocktail for animal immunization. The use of short antigenic peptides of known sequence facilitates direct epitope mapping requiring only small mutagenesis scan peptide libraries in the multipin peptide format., (© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.)

Published
2023
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