Search

Your search keyword '"Lim, Bing"' showing total 548 results
Start Over Author "Lim, Bing"
548 results on '"Lim, Bing"'

3. Immune cell phenotypes associated with disease severity and long-term neutralizing antibody titers after natural dengue virus infection

Author

Rouers, Angeline, Chng, Melissa Hui Yen, Lee, Bernett, Rajapakse, Menaka P., Kaur, Kaval, Toh, Ying Xiu, Sathiakumar, Durgalakshmi, Loy, Thomas, Thein, Tun-Linn, Lim, Vanessa W.X., Singhal, Amit, Yeo, Tsin Wen, Leo, Yee-Sin, Vora, Kalpit A., Casimiro, Danilo, Lim, Bing, Tucker-Kellogg, Lisa, Rivino, Laura, Newell, Evan W., and Fink, Katja

Published
2021
Full Text
View/download PDF

8. A Roadmap for Human Liver Differentiation from Pluripotent Stem Cells

Author

Ang, Lay Teng, Tan, Antson Kiat Yee, Autio, Matias I., Goh, Su Hua, Choo, Siew Hua, Lee, Kian Leong, Tan, Jianmin, Pan, Bangfen, Lee, Jane Jia Hui, Lum, Jen Jen, Lim, Christina Ying Yan, Yeo, Isabelle Kai Xin, Wong, Chloe Jin Yee, Liu, Min, Oh, Jueween Ling Li, Chia, Cheryl Pei Lynn, Loh, Chet Hong, Chen, Angela, Chen, Qingfeng, Weissman, Irving L., Loh, Kyle M., and Lim, Bing

Published
2018
Full Text
View/download PDF

9. Methionine is a metabolic dependency of tumor-initiating cells

Author

Wang, Zhenxun, Yip, Lian Yee, Lee, Jia Hui Jane, Wu, Zhengwei, Chew, Hui Yi, Chong, Pooi Kiat William, Teo, Chin Chye, Ang, Heather Yin-Kuan, Peh, Kai Lay Esther, Yuan, Ju, Ma, Siming, Choo, Li Shi Kimberly, Basri, Nurhidayah, Jiang, Xia, Yu, Qiang, Hillmer, Axel M., Lim, Wan Teck, Lim, Tony Kiat Hon, Takano, Angela, Tan, Eng Huat, Tan, Daniel Shao Weng, Ho, Ying Swan, Lim, Bing, and Tam, Wai Leong

Published
2019
Full Text
View/download PDF

17. Efficient Endoderm Induction from Human Pluripotent Stem Cells by Logically Directing Signals Controlling Lineage Bifurcations

Author

Loh, Kyle M., Ang, Lay Teng, Zhang, Jingyao, Kumar, Vibhor, Ang, Jasmin, Auyeong, Jun Qiang, Lee, Kian Leong, Choo, Siew Hua, Lim, Christina Y.Y., Nichane, Massimo, Tan, Junru, Noghabi, Monireh Soroush, Azzola, Lisa, Ng, Elizabeth S., Durruthy-Durruthy, Jens, Sebastiano, Vittorio, Poellinger, Lorenz, Elefanty, Andrew G., Stanley, Edouard G., Chen, Qingfeng, Prabhakar, Shyam, Weissman, Irving L., and Lim, Bing

Published
2014
Full Text
View/download PDF

21. Publisher Correction: Methionine is a metabolic dependency of tumor-initiating cells

Author

Wang, Zhenxun, Yip, Lian Yee, Lee, Jia Hui Jane, Wu, Zhengwei, Chew, Hui Yi, Chong, Pooi Kiat William, Teo, Chin Chye, Ang, Heather Yin-Kuan, Peh, Kai Lay Esther, Yuan, Ju, Ma, Siming, Choo, Li Shi Kimberly, Basri, Nurhidayah, Jiang, Xia, Yu, Qiang, Hillmer, Axel M., Lim, Wan Teck, Lim, Tony Kiat Hon, Takano, Angela, Tan, Eng Huat, Tan, Daniel Shao Weng, Ho, Ying Swan, Lim, Bing, and Tam, Wai Leong

Published
2019
Full Text
View/download PDF

22. Glycine Decarboxylase Activity Drives Non-Small Cell Lung Cancer Tumor-Initiating Cells and Tumorigenesis

Author

Zhang, Wen Cai, Shyh-Chang, Ng, Yang, He, Rai, Amit, Umashankar, Shivshankar, Ma, Siming, Soh, Boon Seng, Sun, Li Li, Tai, Bee Choo, Nga, Min En, Bhakoo, Kishore Kumar, Jayapal, Senthil Raja, Nichane, Massimo, Yu, Qiang, Ahmed, Dokeu A., Tan, Christie, Sing, Wong Poo, Tam, John, Thirugananam, Agasthian, Noghabi, Monireh Soroush, Huei Pang, Yin, Ang, Haw Siang, Mitchell, Wayne, Robson, Paul, Kaldis, Philipp, Soo, Ross Andrew, Swarup, Sanjay, Lim, Elaine Hsuen, and Lim, Bing

Published
2012
Full Text
View/download PDF

24. Computer and Statistical Analysis of Transcription Factor Binding and Chromatin Modifications by ChIP-seq data in Embryonic Stem Cell

Author

Orlov Yuriy, Xu Han, Afonnikov Dmitri, Lim Bing, Heng Jian-Chien, Yuan Ping, Chen Ming, Yan Junli, Clarke Neil, Orlova Nina, Huss Mikael, Gunbin Konstantin, Podkolodnyy Nikolay, and Ng Huck-Hui

Subjects
Biotechnology, TP248.13-248.65
Abstract

Advances in high throughput sequencing technology have enabled the identification of transcription factor (TF) binding sites in genome scale. TF binding studies are important for medical applications and stem cell research. Somatic cells can be reprogrammed to a pluripotent state by the combined introduction of factors such as Oct4, Sox2, c-Myc, Klf4. These reprogrammed cells share many characteristics with embryonic stem cells (ESCs) and are known as induced pluripotent stem cells (iPSCs). The signaling requirements for maintenance of human and murine embryonic stem cells (ESCs) differ considerably. Genome wide ChIP-seq TF binding maps in mouse stem cells include Oct4, Sox2, Nanog, Tbx3, Smad2 as well as group of other factors. ChIP-seq allows study of new candidate transcription factors for reprogramming. It was shown that Nr5a2 could replace Oct4 for reprogramming. Epigenetic modifications play important role in regulation of gene expression adding additional complexity to transcription network functioning. We have studied associations between different histone modification using published data together with RNA Pol II sites. We found strong associations between activation marks and TF binding sites and present it qualitatively. To meet issues of statistical analysis of genome ChIP-sequencing maps we developed computer program to filter out noise signals and find significant association between binding site affinity and number of sequence reads. The data provide new insights into the function of chromatin organization and regulation in stem cells.

Published
2012
Full Text
View/download PDF

28. The Transcriptome of Human Oocytes

Author

Kocabas, Arif Murat, Crosby, Javier, Ross, Pablo J., Otu, Hasan H., Beyhan, Zeki, Can, Handan, Tam, Wai-Leong, Rosa, Guilherme J. M., Halgren, Robert G., Lim, Bing, Fernandez, Emilio, and Cibelli, Jose Bernardo

Published
2006
Full Text
View/download PDF

41. ZFP36L2 is required for self-renewal of early burst-forming unit erythroid progenitors

Author

Zhang, Lingbo, Prak, Lina, Rayon-Estrada, Violeta, Thiru, Prathapan, Flygare, Johan, Lim, Bing, and Lodish, Harvey F.

Subjects
Binding proteins -- Properties, Erythrocytes -- Properties, Cell differentiation, Environmental issues, Science and technology, Zoology and wildlife conservation
Abstract

Stem cells and progenitors in many lineages undergo self-renewing divisions, but the extracellular and intracellular proteins that regulate this process are largely unknown. Glucocorticoids stimulate red blood cell formation by promoting self-renewal of early burst-forming unit-erythroid (BFU-E) progenitors (1-4). Here we show that the RNA-binding protein ZFP36L2 is a transcriptional target of the glucocorticoid receptor (GR) in BFU-Es and is required for BFU-E self-renewal. ZFP36L2 is normally downregulated during erythroid differentiation from the BFU-E stage, but its expression is maintained by all tested GR agonists that stimulate BFU-E self-renewal, and the GR binds to several potential enhancer regions of ZFP36L2. Knockdown of ZFP36L2 in cultured BFU-E cells did not affect the rate of cell division but disrupted glucocorticoid-induced BFU-E self-renewal, and knockdown of ZFP36L2 in transplanted erythroid progenitors prevented expansion of erythroid lineage progenitors normally seen following induction of anaemia by phenylhydrazine treatment. ZFP36L2 preferentially binds to messenger RNAs that are induced or maintained at high expression levels during terminal erythroid differentiation and negatively regulates their expression levels. ZFP36L2 therefore functions as part of a molecular switch promoting BFU-E self-renewal and a subsequent increase in the total numbers of colony-forming unit-erythroid (CFU-E) progenitors and erythroid cells that are generated., Humans generate 10 (11) erythrocytes every day, a process regulated by multiple hormones affecting several types of progenitors. Apoptosis, proliferation and terminal differentiation of CFU-E erythroid progenitors are mainly controlled [...]

Published
2013

45. A genome-wide RNAi screen reveals determinants of human embryonic stem cell identity

Author

Chia, Na-Yu, Chan, Yun-Shen, Feng, Bo, Lu, Xinyi, Orlov, Yuriy L., Moreau, Dimitri, Kumar, Pankaj, Yang, Lin, Jiang, Jianming, Lau, Mei-Sheng, Huss, Mikael, Soh, Boon-Seng, Kraus, Petra, Li, Pin, Lufkin, Thomas, Lim, Bing, Clarke, Neil D., Bard, Frederic, and Ng, Huck-Hui

Subjects
Embryonic stem cells -- Physiological aspects, Stem cell research, Environmental issues, Science and technology, Zoology and wildlife conservation
Abstract

The derivation of human ES cells (hESCs) from human blastocysts represents one of the milestones in stem cell biology (1). The full potential of hESCs in research and clinical applications [...]

Published
2010
Full Text
View/download PDF

46. Tbx3 improves the germ-line competency of induced pluripotent stem cells

Author

Jianyong, Han, Yuan, Ping, Yang, Henry, Zhang, Jinqiu, Soh, Boon Seng, Li, Pin, Lim, Siew Lan, Cao, Suying, Tay, Junliang, Orlov, Yuriy L., Lufkin, Thomas, Ng, Huck-Hui, Tam, Wai-Leong, and Lim, Bing

Subjects
Embryonic stem cells -- Analysis -- Physiological aspects -- Genetic aspects, Cell differentiation -- Physiological aspects -- Genetic aspects -- Research, Transcription factors -- Physiological aspects, Environmental issues, Science and technology, Zoology and wildlife conservation
Abstract

Induced pluripotent stem (iPS) cells can be obtained by the introduction of defined factors into somatic cells (1). The combination of Oct4 (also known as Pou5f1), Sox2 and Klf4 (which we term OSK) constitutes the minimal requirement for generating iPS cells from mouse embryonic fibroblasts. These cells are thought to resemble embryonic stem cells (ESCs) on the basis of global gene expression analyses; however, few studies have tested the ability and efficiency of iPS cells to contribute to chimaerism, colonization of germ tissues, and most importantly, germ-line transmission and live birth from iPS cells produced by tetraploid complementation. Using genomic analyses of ESC genes that have roles in pluripotency and fusion-mediated somatic cell reprogramming, here we show that the transcription factor Tbx3 significantly improves the quality of iPS cells. iPS cells generated with OSK and Tbx3 (OSKT) are superior in both germ-cell contribution to the gonads and germ-line transmission frequency. However, global gene expression profiling could not distinguish between OSK and OSKT iPS cells. Genome-wide chromatin immunoprecipitation sequencing analysis of Tbx3-binding sites in ESCs suggests that Tbx3 regulates pluripotency-associated and reprogramming factors, in addition to sharing many common downstream regulatory targets with Oct4, Sox2, Nanog and Smad1. This study underscores the intrinsic qualitative differences between iPS cells generated by different methods, and highlights the need to rigorously characterize iPS cells beyond in vitro studies., The pluripotency and self-renewing properties of ESCs are conferred by a set of core factors that helps determine their unique identity. Adult somatic cells can be reprogrammed to resemble ESCs [...]

Published
2010
Full Text
View/download PDF

48. RCP is a human breast cancer--promoting gene with Ras-activating function

Author

Zhang, Jinqiu, Liu, Xuejing, Datta, Arpita, Govindarajan, Kunde, Tam, Wai Leong, Han, Jianyong, George, Joshy, Wong, Christopher, Ramnarayanan, Kalpana, Phua, Tze Yoong, Leong, Wan Yee, Chan, Yang Sun, Palanisamy, Nallasivam, Liu, Edison Tak-Bun, Karuturi, Krishna Murthy, Lim, Bing, and Miller, Lance David

Subjects
Breast cancer -- Genetic aspects, Breast cancer -- Development and progression, Breast cancer -- Research, DNA microarrays -- Usage, Cancer -- Genetic aspects, Cancer -- Research
Abstract

Aggressive forms of cancer are often defined by recurrent chromosomal alterations, yet in most cases, the causal or contributing genetic components remain poorly understood. Here, we utilized microarray informatics to identify candidate oncogenes potentially contributing to aggressive breast cancer behavior. We identified the Rab-coupling protein RCP (also known as RAB11FIP1), which is located at a chromosomal region frequently amplified in breast cancer (8p11-12) as a potential candidate. Overexpression of RCP in MCF10A normal human mammary epithelial cells resulted in acquisition of tumorigenic properties such as loss of contact inhibition, growth-factor independence, and anchorage-independent growth. Conversely, knockdown of RCP in human breast cancer cell lines inhibited colony formation, invasion, and migration in vitro and markedly reduced tumor formation and metastasis in mouse xenograft models. Overexpression of RCP enhanced ERK phosphorylation and increased Ras activation in vitro. As these results indicate that RCP is a multifunctional gene frequently amplified in breast cancer that encodes a protein with Ras-activating function, we suggest it has potential importance as a therapeutic target. Furthermore, these studies provide new insight into the emerging role of the Rab family of small G proteins and their interacting partners in carcinogenesis., Introduction The malignant growth of cancer is fueled in part by pathological alterations of the genome that reconfigure the transcriptional programming of cells. This transcriptional restructuring gives rise to the [...]

Published
2009

49. MicroRNAs to Nanog, Oct4 and Sox2 coding regions modulate embryonic stem cell differentiation

Author

Tay, Yvonne, Zhang, Jingiu, Thomson, Andrew M., Lim, Bing, and Rigoutsos, Isidore

Subjects
Messenger RNA -- Research -- Reports, Cell differentiation -- Research -- Reports, Stem cell research -- Reports -- Research, Environmental issues, Science and technology, Zoology and wildlife conservation, Research, Reports
Abstract

MicroRNAs (miRNAs) are short RNAs that direct messenger RNA degradation or disrupt mRNA translation in a sequence-dependent manner (1-7). For more than a decade, attempts to study the interaction of [...]

Published
2008

50. Genomic landscape of lung adenocarcinoma in East Asians

Author

Chen, Jianbin, Yang, Hechuan, Teo, Audrey Su Min, Amer, Lidyana Bte, Sherbaf, Faranak Ghazi, Tan, Chu Quan, Alvarez, Jacob Josiah Santiago, Lu, Bingxin, Lim, Jia Qi, Takano, Angela, Nahar, Rahul, Lee, Yin Yeng, Phual, Cheryl Zi Jin, Chua, Khi Pin, Suteja, Lisda, Chen, Pauline Jieqi, Chang, Mei Mei, Koh, Tina Puay Theng, Ong, Boon-Hean, Anantham, Devanand, Hsu, Anne Ann Ling, Gogna, Apoorva, Too, Chow Wei, Aung, Zaw Win, Lee, Yi Fei, Wang, Lanying, Lim, Tony Kiat Hon, Wilm, Andreas, Choi, Poh Sum, Ng, Poh Yong, Toh, Chee Keong, Lim, Wan-Teck, Ma, Siming, Lim, Bing, Liu, Jin, Tam, Wai Leong, Skanderup, Anders Jacobsen, Yeong, Joe Poh Sheng, Tan, Eng-Huat, Creasy, Caretha L., Tan, Daniel Shao Weng, Hillmer, Axel M., Zhai, Weiwei, Chen, Jianbin, Yang, Hechuan, Teo, Audrey Su Min, Amer, Lidyana Bte, Sherbaf, Faranak Ghazi, Tan, Chu Quan, Alvarez, Jacob Josiah Santiago, Lu, Bingxin, Lim, Jia Qi, Takano, Angela, Nahar, Rahul, Lee, Yin Yeng, Phual, Cheryl Zi Jin, Chua, Khi Pin, Suteja, Lisda, Chen, Pauline Jieqi, Chang, Mei Mei, Koh, Tina Puay Theng, Ong, Boon-Hean, Anantham, Devanand, Hsu, Anne Ann Ling, Gogna, Apoorva, Too, Chow Wei, Aung, Zaw Win, Lee, Yi Fei, Wang, Lanying, Lim, Tony Kiat Hon, Wilm, Andreas, Choi, Poh Sum, Ng, Poh Yong, Toh, Chee Keong, Lim, Wan-Teck, Ma, Siming, Lim, Bing, Liu, Jin, Tam, Wai Leong, Skanderup, Anders Jacobsen, Yeong, Joe Poh Sheng, Tan, Eng-Huat, Creasy, Caretha L., Tan, Daniel Shao Weng, Hillmer, Axel M., and Zhai, Weiwei

Abstract

Lung cancer is the world's leading cause of cancer death and shows strong ancestry disparities. By sequencing and assembling a large genomic and transcriptomic dataset of lung adenocarcinoma (LUAD) in individuals of East Asian ancestry (EAS; n = 305), we found that East Asian LUADs had more stable genomes characterized by fewer mutations and fewer copy number alterations than LUADs from individuals of European ancestry. This difference is much stronger in smokers as compared to nonsmokers. Transcriptomic clustering identified a new EAS-specific LUAD subgroup with a less complex genomic profile and upregulated immune-related genes, allowing the possibility of immunotherapy-based approaches. Integrative analysis across clinical and molecular features showed the importance of molecular phenotypes in patient prognostic stratification. EAS LUADs had better prediction accuracy than those of European ancestry, potentially due to their less complex genomic architecture. This study elucidated a comprehensive genomic landscape of EAS LUADs and highlighted important ancestry differences between the two cohorts. Genomic and transcriptomic analysis of lung adenocarcinoma (LUAD) in Asia indicates that Asian LUADs have fewer mutations, lower driver prevalence and fewer copy number alterations than European LUADs.

Published
2020

Catalog

Books, media, physical & digital resources
See catalog results