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Your search keyword '"Limonium serotinum"' showing total 2 results
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2 results on '"Limonium serotinum"'

1. Cryopreservation of Limonium serotinum apical meristems from in vitro plantlets using droplet-vitrification

Author

Barraco, Giuseppe, Sylvestre, Isabelle, Iapichino, Giovanni, and Engelmann, Florent

Subjects
*LIMONIUM, *PLANT genetics, *PLANT shoots, *SUCROSE, *GLYCERIN, *DIMETHYL sulfoxide, *ETHYLENE glycol, *PLANT growth, *CRYOPRESERVATION of plant cells & tissues, *SOLUTION (Chemistry)
Abstract

Abstract: In this study in vitro shoot tips of a Sicilian genotype of Limonium serotinum were successfully cryopreserved using the droplet-vitrification technique. Growth recovery of cryopreserved shoot tips was possible only when samples were pretreated for 16h in liquid medium with 0.3M sucrose, then for 5h in liquid medium with 0.7M sucrose before performing the cryopreservation protocol. Optimal conditions included treatment for 20min in a loading solution containing 1.9M glycerol+0.5M sucrose, treatment with vitrification solution B5 (glycerol 40.0%, sucrose 40.0%, w/v) for 60 and 90min or vitrification solution A9 (glycerol 30.0%, dimethylsulfoxide 20.0%, ethylene glycol 20.0%, sucrose 15.0%) for 20min, rapid cooling in minute droplets of vitrification solution, rapid rewarming by immersion for 20min in unloading solution containing 1.2M sucrose. Under these conditions, 37% recovery of cryopreserved shoot tips was achieved. Regrowth of cryopreserved samples was slow but always direct, without callus formation. [Copyright &y& Elsevier]

Published
2011
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2. Cryopreservation of Limonium serotinum apical meristems from in vitro plantlets using droplet-vitrification

Author

Giuseppe Barraco, Isabelle Sylvestre, Giovanni Iapichino, Florent Engelmann, Barraco, G, Sylvestre, I, Iapichino, G, and Engelmann, F

Subjects
Cryopreservation, Sucrose, Chromatography, Callus formation, Limonium, Settore AGR/04 - Orticoltura E Floricoltura, Horticulture, Biology, Limonium serotinum, Vitrification solution, biology.organism_classification, chemistry.chemical_compound, Cryopreservation Droplet-vitrification Limonium serotinum Statice Sucrose pretreatment Vitrification solution, chemistry, Droplet-vitrification, Shoot, Botany, Glycerol, Statice, Sucrose pretreatment, Vitrification, Ethylene glycol
Abstract

In this study in vitro shoot tips of a Sicilian genotype of Limonium serotinum were successfully cryopreserved using the droplet-vitrification technique. Growth recovery of cryopreserved shoot tips was possible only when samples were pretreated for 16 h in liquid medium with 0.3 M sucrose, then for 5 h in liquid medium with 0.7 M sucrose before performing the cryopreservation protocol. Optimal conditions included treatment for 20 min in a loading solution containing 1.9 M glycerol + 0.5 M sucrose, treatment with vitrification solution B5 (glycerol 40.0%, sucrose 40.0%, w/v) for 60 and 90 min or vitrification solution A9 (glycerol 30.0%, dimethylsulfoxide 20.0%, ethylene glycol 20.0%, sucrose 15.0%) for 20 min, rapid cooling in minute droplets of vitrification solution, rapid rewarming by immersion for 20 min in unloading solution containing 1.2 M sucrose. Under these conditions, 37% recovery of cryopreserved shoot tips was achieved. Regrowth of cryopreserved samples was slow but always direct, without callus formation.

Published
2011

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