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13 results on '"Lin, Qiu-Xia"'

3. Carbapenem-Resistant Klebsiella aerogenes Clinical Isolates from a Teaching Hospital in Southwestern China: Detailed Molecular Epidemiology, Resistance Determinants, Risk Factors and Clinical Outcomes

Author

Ma,De-Yu, Huang,Han-Yu, Zou,Hua, Wu,Meng-Lu, Lin,Qiu-Xia, Liu,Bo, and Huang,Shi-Feng

Subjects
Infection and Drug Resistance
Abstract

De-Yu Ma, 1,* Han-Yu Huang, 2,* Hua Zou, 1 Meng-Lu Wu, 1 Qiu-Xia Lin, 1 Bo Liu, 3 Shi-Feng Huang 1 1Department of Laboratory Medicine, The First Affiliated Hospital of Chongqing Medical University, Chongqing, People’s Republic of China; 2Department of Radiology, Chongqing Health Center for Women and Children, Chongqing, People’s Republic of China; 3Department of Burn and Plastic Surgery, The First Affiliated Hospital of Chongqing Medical University, Chongqing, People’s Republic of China*These authors contributed equally to this workCorrespondence: Bo LiuDepartment of Burn and Plastic Surgery, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China, No. 1 Friendship Road, Yuzhong District, Chongqing 400016, People’s Republic of ChinaTel +86-18623007069Fax +86-023-89012093Email fcz69@sina.com Shi-Feng HuangDepartment of Clinical Laboratory, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China, No. 1 Friendship Road, Yuzhong District, Chongqing 400016, People’s Republic of ChinaTel +86-18623027077Fax +86-023-89012513Email sfhuang@hospital.cqmu.edu.cnPurpose: Little is known about the epidemiology and carbapenem-resistance determinants of carbapenem-resistant K. aerogenes (CRKA) isolated from a single medical center. The present study was initiated to characterize the molecular epidemiology and the carbapenem-resistance mechanisms of CRKA isolated during 2012– 2018 from a teaching hospital in southwest China, and to investigate the risk factors and clinical outcomes of CRKA infections as well.Methods: Pulsed-field gel electrophoresis (PFGE) was employed for epidemiological analysis. PCR amplification and DNA sequencing were used to examine the antibiotic-resistance determinants. Plasmids were extracted and characterized by PCR-based replicon typing and conjugation assays. In order to further investigate the risk factors and clinical outcomes of CRKA infections, a retrospective case–control study was also performed.Results: PFGE analysis showed 32 different PFGE patterns among the 36 non-duplicated CRKA strains collected. Most of the isolates harbored multi-drug resistance (MDR) genes, including 2 (5.6%) carrying blaNDM-1, 1 (2.8%) harboring blaKPC-2, 13 (36.1%) carrying ESBL genes, 23 (63.9%) carrying ampC genes, 34 (94.4%) carrying quinolone resistance determinants (QRD) genes and 9 (25%) carrying aminoglycoside resistance determinants (ARD) genes. The outer membrane porins, OmpE35 and OmpE36, were, respectively, lost in 4 and 2 isolates. The efflux pump inhibition experiments were positive in 25 (69.4%) of the CRKA strains. Multivariate analysis indicated that hypo-albuminaemia, invasive procedures, and carbapenem exposure were independent risk factors for acquiring CRKA infections.Conclusion: No clonality relationship was identified among most of the 36 CRKA isolates. The over-expression of ESBLs and AmpC coupled with the efflux pumps contributed to carbapenem resistance in K. aerogenes. Additionally, this is the first report of CRKA isolate co-harboring blaNDM-1, blaCTX-M-15, blaEBC, blaACC, acc (6ʹ)-Ib, armA, qnrD and loss of OmpE36 in China. Hypo-albuminaemia, invasive procedures and carbapenem exposure were associated with acquisition of CRKA infections.Keywords: case-control study, blaNDM-1, blaKPC-2, porins, efflux pumps

Published
2020

4. CP-CRE/non-CP-CRE Stratification And CRE Resistance Mechanism Determination Help In Better Managing CRE Bacteremia Using Ceftazidime–Avibactam And Aztreonam–Avibactam

Author

Zou,Hua, Xiong,Sen-Jie, Lin,Qiu-Xia, Wu,Meng-Lu, Niu,Si-Qiang, and Huang,Shi-Feng

Subjects
Infection and Drug Resistance
Abstract

Hua Zou,1,* Sen-Jie Xiong,2,* Qiu-Xia Lin,1,* Meng-Lu Wu,1 Si-Qiang Niu,1 Shi-Feng Huang1 1Department of Laboratory Medicine, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, People’s Republic of China; 2Department of Neurosurgery, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, People’s Republic of China*These authors contributed equally to this workCorrespondence: Si-Qiang Niu; Shifeng HuangDepartment of Laboratory Medicine, The First Affiliated Hospital of Chongqing Medical University, No. 1 Friendship Road, Yuzhong District, Chongqing 400016, People’s Republic of ChinaTel +86 23 8901 2742; Tel +86 186 230 27077Fax +86 23 8901 2513Email siqiangniu@cqmu.edu.cn; sfhuang@hospital.cqmu.edu.cnPurpose: This observational study aimed to identify the independent risk factors for both the acquisition and mortality of carbapenemase-producing carbapenem-resistant Enterobacteriaceae (CP-CRE) bacteremia and further assess the in vitro antimicrobial activities of ceftazidime–avibactam (CAZ/AVI) and aztreonam–avibactam (ATM/AVI) against recent CRE bacteremic isolates.Patients and methods: This observational study was conducted to reveal the risk factors and mortality rate for CP-CRE bacteremia between 2012 and 2018 and also evaluate the in vitro antimicrobial activities of CAZ/AVI and ATM/AVI against recent CRE bacteremic isolates from 2016 to 2018.Results: A total of 81 non-repetitive isolates were collected from 2012 to 2018, with 67.90% (55/81) being CP-CRE. Old age (P = 0.01), transfusion [odds ratio (OR): 17.19; 95% CI: 3.15–93.72; P = 0.001], longer ICU stay (P = 0.02), cancer (OR: 15.91; 95% CI: 3.56–71.37; P < 0.001), and previous carbapenem exposure (OR: 27.86; 95% CI: 5.03–154.19; P = 0.001) were identified as independent risk factors for the acquisition of CP-CRE bacteremia compared with the ESBL bacteremia. The in vitro antimicrobial activities of CAZ/AVI and ATM/AVI against the CRE bacteremic isolates from 2016 to 2018 showed a respective susceptibility rate of 70.68% (41/58) and 100.00% (58/58).Conclusion: The findings indicated that both CP-CRE/non-CP-CRE stratification and CRE resistance mechanism determination were necessary for better guiding the clinical management of CRE bacteremia: ATM/AVI probably works with both non-CP-CRE and CP-CRE bacteremia, even the most notorious double-carbapenemase producer with porin loss/deficiency, whereas CAZ/AVI works with most of the non-CP-CRE and KPC-producers in the region.Keywords: aztreonam–avibactam, carbapenemase-producing carbapenem-resistant Enterobacteriaceae, carbapenem-resistant Enterobacteriaceae bacteremia, ceftazidime–avibactam

Published
2019

10. Reconstruction of Engineered Uterine Tissues Containing Smooth Muscle Layer in Collagen/Matrigel Scaffold In Vitro

Author

Lü, Shuang-Hong, Wang, Hai-Bin, Liu, Hui, Wang, He-Ping, Lin, Qiu-Xia, Li, De-Xue, Song, Yu-Xuan, Duan, Cui-Mi, Feng, Li-Xin, and Wang, Chang-Yong

Abstract

Objective:This study attempted to reconstruct engineered uterine tissues (EUTs) containing smooth muscle layer, akin to the normal uterine wall. Methods: EUTs were reconstructed by seeding epithelial cells on top of the constructed stromal layer over smooth muscle layer. A self-made mold was used to keep the EUTs from contraction. At the same time, it provided static stretch to the EUTs. After 14 days of culture, the structure of the EUTs was analyzed histologically and immunohistochemically, or by scanning electron microscopy (SEM) and transmission electron microscopy (TEM). The expression of integrin β3 subunit, heparin-binding epidermal growth factor (EGF)–like growth factor (HB-EGF), and HOXA-10was detected by reverse transcription–polymerase chain reaction (RT-PCR). The ability of the EUTs supporting the development of embryos was estimated by coculturing embryos on the EUTs. We also tried a new method to reconstruct EUTs by mixing epithelial cell and stromal cells (1:2) in collagen/Matrigel to form an endometrial layer and putting it on top of the smooth muscle layer. The self-assembling ability of the endometrial epithelial cells and stromal cells in the reconstructed EUTs was analyzed histologically and immunohistochemically. Results: The results found that the constructed EUTs with the first and the second method showed three-layered structures. The epithelial layer, stromal layer, and smooth muscle layer were stained by cytokeratin 18, vimentin, and α-actin, respectively. TEM showed that the cells in the EUTs reconstructed by the first method were attached to each other by apical tight junctions and rivet-like desmosomes. SEM showed protruded pinopodes, microvilli, and cilium of epithelial cells. The RT-PCR analysis showed that integrin β3 subunit, HB-EGF, and HOXA-10were expressed in EUTs. The coculture system of EUTs improved the development rate and quality of murine embryo significantly in comparison with those of control Chatot Ziomek Bavister culture. In the EUTs reconstructed by the second method, the epithelial cells demonstrated self-assembling ability and formed epithelial cell layer on top of the stromal layer and glandular tube–like structures in the stromal layer. Columnar epithelial cells existed in some parts of the epithelial layer. Conclusion: We engineered EUTs containing smooth muscle layer by two methods. The reconstructed EUTs could support the development of embryos. The epithelial cells showed self-assembling ability in the EUTs.

Published
2009
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11. cDNA microarray analysis of spinal cord injury and regeneration related genes in rat.

Author

Xiao L, Ma ZL, Li X, Lin QX, Que HP, and Liu SJ

Subjects
Animals, Expressed Sequence Tags, Gene Expression Regulation, Male, Oligonucleotide Array Sequence Analysis, Random Allocation, Rats, Rats, Wistar, Reverse Transcriptase Polymerase Chain Reaction, Spinal Cord Injuries physiopathology, Gene Expression Profiling, Nerve Tissue Proteins genetics, Spinal Cord Injuries genetics, Spinal Cord Regeneration genetics
Abstract

The acute traumatic spinal cord injury (SCI) is a commonly seen and severe case in clinic. However, the repair and regeneration of injured spinal cord is limited. This is likely due to that different kinds of factors are involved in regeneration after SCI. In the present study, we used complementary DNA microarray consisting of 4 041 specific probes from rat to identify genes that were differentially expressed after SCI. The animals were subjected to complete transection injury of the thoracic spinal cord (T8-T9). Sham operated animals received only a laminectomy. Four and a half days later, rat spinal cord was dissected out for total RNA isolation. The fluorescent (Cy3 and Cy5) labeled probes were prepared and hybridized to the microarray. Genes that showed 2-fold difference in SCI tissue were identified. Sixty-five up-regulated genes consisted of 21 known genes, 30 known expressed sequence tags (ESTs) and 14 unknown genes. Seventy-nine down-regulated genes comprised 20 known genes, 42 known ESTs and 17 unknown genes. In 41 differentially expressed known genes, 5 up-regulated genes, i.e., tissue inhibitor of metalloproteinase 1 (Timp1), transgelin (Tagln), vimentin (Vim), Fc gamma receptor, cathepsin S (Ctss), and 3 down-regulated genes, i.e., stearyl-CoA desaturase, coagulation factor II (F2), endosulfin alpha (Ensa), were further confirmed by reverse transcription polymerase chain reaction (RT-PCR). These genes may play a role in the response to tissue damage or repair following SCI and characterization of them might be helpful to elucidate the molecular mechanisms of spinal cord injury and regeneration.

Published
2005

12. [Progress of research on stem cell plasticity].

Author

Lin QX and Liu SJ

Subjects
Animals, Gene Expression Regulation, Developmental, Humans, Multipotent Stem Cells cytology, Multipotent Stem Cells physiology, Neurons physiology, Pluripotent Stem Cells cytology, Pluripotent Stem Cells physiology, Stem Cell Transplantation, Stem Cells physiology, Cell Differentiation physiology, Neuronal Plasticity, Stem Cells cytology
Published
2004

13. [Induced-division of neurons derived from neural stem cells].

Author

Lin QX, Que HP, Lu SH, and Liu SJ

Subjects
Animals, Brain cytology, Cell Division, Cell Separation, Cells, Cultured, Epidermal Growth Factor pharmacology, Fibroblast Growth Factor 2 pharmacology, Photography methods, Proliferating Cell Nuclear Antigen pharmacology, Rats, Rats, Wistar, Cell Differentiation, Neurons cytology, Stem Cells cytology
Abstract

In order to explore if mature neurons derived from neural stem cells have the potentiality to divide, we utilized the chemical digestion method to disperse the adult rat brain tissue into single cells, and culture them in serum-free medium. After being cultured for about eight days in vitro, the neural stem cells were induced to differentiate into neurons. The neurons were further induced to divide. Utilizing the method of serial photograph and NF-160 immunocytochemistry, the processes of division of some neurons were recorded. At the same time, PCNA+NF-160 (or Chat, GABA, GAD) double label were used to investigate if the dividing-neurons were mature ones. After the neural stem cells were induced to differentiate in vitro for eight days, they possessed the shape and character of mature neurons. The differentiated neuron had a big nucleus and one or two distinct nucleolus in the nuclear. Within the perikaryon,there were a large amount of dense and Nissl body-like structure. Several long processes emerged from various locations of the cell body. Then, EGF and bFGF were added into the medium to induce division. After two days of induced-division, neuron-like cells were observed to divide; moreover, the number of neuron-like cells in the region increased continually. Immunocytochemistry demonstrated these cells were NF-160-positive. Serial photographs of dividing-process of neuron-like cells were obtained and their daughter cells were also NF-160-positive. After PCNA+NF-160 (or Chat, GABA, GAD) double label, some cells showed brown cell plasma and black nucleus. The above-mentioned results indicate that neurons, which were previously thought to be end-differentiated, can be re-called into cell cycle under appropriate conditions. Mature neurons still have the potential to divide, proliferate and self-renew.

Published
2004

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