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16. Surface Properties of Aqueous Dispersions of Bovine Serum Albumin Fibrils

Author

Akentiev, Alexander, Lin, Shi-Yow, Loglio, Giuseppe, Miller, Reinhard, Noskov, Boris, Akentiev, Alexander, Lin, Shi-Yow, Loglio, Giuseppe, Miller, Reinhard, and Noskov, Boris

Abstract

The surface properties of aqueous dispersions of worm-like fibril aggregates of bovine serum albumin (BSA) differ from those of the adsorption layers of the native protein. The dispersions of BSA fibrils are characterized by slower changes of the surface tension and dynamic surface elasticity and also have different steady-state values of the surface properties. The fourfold compression of the adsorption layer of BSA fibrils leads to noticeably higher surface pressures than those of a compressed layer of the native protein, indicating the formation of a more rigid layer structure in the former case. The spreading of BSA fibrils onto a liquid surface from a concentrated dispersion reduces the effect of surface-active admixtures on the layer properties. The dependencies of the dynamic surface elasticity on surface pressure almost coincide for the spread layers of fibrils and the native protein in the range of low surface pressures, but only the spreading of the native protein can lead to surface pressures higher than 4 mN/m. This distinction is presumably caused by the formation of stable clusters of BSA fibrils at the interface and their slow propagation along the liquid surface.

Published
2024

36. Measurement of Dilational Modulus of an Adsorbed BSA Film Using Pendant Bubble Tensiometry: From a Clean Interface to Saturation.

Author

Hussain, Siam, Rivas, Johann Eduardo Maradiaga, Tseng, Wen-Chi, Tsay, Ruey-Yug, Noskov, Boris, Loglio, Giuseppe, and Lin, Shi-Yow

Subjects
AIR-water interfaces, SURFACE tension, SURFACE area, ADSORPTION (Chemistry), SERUM albumin
Abstract

Two open issues on the measurement of the dilational modulus (E) for an adsorbed protein film during the adsorption process have been unacknowledged: how E varies during the adsorption and the length of time needed to attain a stable E value. A new approach for detecting the E variation from a clean air–water interface to saturated film and estimating the time needed to reach a saturated state was proposed. A pendant bubble tensiometer was utilized for measuring the relaxations of surface tension (ST) and surface area (SA), and the E was evaluated from the relaxation data of minute distinct perturbances. The data showed a clear variation in E during the BSA adsorption: E sharply decreased to a minimum at the early stage of BSA adsorption; then, it rose from this minimum and oscillated for a while before reaching an E corresponding to a saturated BSA film after a significant duration. The adsorbed BSA film took ~35 h to reach its saturated state, which was much longer than the reported lifetime of the adsorbed film in the literature. A rapid surface perturbation (forced bubble expansion/compression) could change the E, causing a significant drop in E followed by a slow increase to the original stable value. [ABSTRACT FROM AUTHOR]

Published
2024
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