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291 results on '"Lin, Wencheng"'

17. Isolation and Pathogenicity Analysis of a G5P[23] Porcine Rotavirus Strain.

Author

Gao, Liguo, Shen, Hanqin, Zhao, Sucan, Chen, Sheng, Zhu, Puduo, Lin, Wencheng, and Chen, Feng

Subjects
ROTAVIRUSES, LIVESTOCK losses, ANIMAL industry, INTESTINAL diseases, VACCINE development, PIGLETS, SWINE farms
Abstract

(1) Background: Group A rotaviruses (RVAs) are the primary cause of severe intestinal diseases in piglets. Porcine rotaviruses (PoRVs) are widely prevalent in Chinese farms, resulting in significant economic losses to the livestock industry. However, isolation of PoRVs is challenging, and their pathogenicity in piglets is not well understood. (2) Methods: We conducted clinical testing on a farm in Jiangsu Province, China, and isolated PoRV by continuously passaging on MA104 cells. Subsequently, the pathogenicity of the isolated strain in piglets was investigated. The piglets of the PoRV-infection group were orally inoculated with 1 mL of 1.0 × 106 TCID50 PoRV, whereas those of the mock-infection group were fed with an equivalent amount of DMEM. (3) Results: A G5P[23] genotype PoRV strain was successfully isolated from one of the positive samples and named RVA/Pig/China/JS/2023/G5P[23](JS). The genomic constellation of this strain was G5-P[23]-I5-R1-C1-M1-A8-N1-T1-E1-H1. Sequence analysis revealed that the genes VP3, VP7, NSP2, and NSP4 of the JS strain were closely related to human RVAs, whereas the remaining gene segments were closely related to porcine RVAs, indicating a reassortment between porcine and human strains. Furthermore, infection of 15-day-old piglets with the JS strain resulted in a diarrheal rate of 100% (8 of 8) and a mortality rate of 37.5% (3 of 8). (4) Conclusions: The isolated G5P[23] genotype rotavirus strain, which exhibited strong pathogenicity in piglets, may have resulted from recombination between porcine and human strains. It may serve as a potential candidate strain for developing vaccines, and its immunogenicity can be tested in future studies. [ABSTRACT FROM AUTHOR]

Published
2024
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32. Chromosome-level genome assembly of goose provides insight into the adaptation and growth of local goose breeds

Author

Zhao, Qiqi, primary, Lin, Zhenping, additional, Chen, Junpeng, additional, Xie, Zi, additional, Wang, Jun, additional, Feng, Keyu, additional, Lin, Wencheng, additional, Li, Hongxin, additional, Hu, Zezhong, additional, Chen, Weiguo, additional, Chen, Feng, additional, Junaid, Muhammad, additional, Zhang, Huanmin, additional, Xie, Qingmei, additional, and Zhang, Xinheng, additional

Published
2022
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38. Molecular Characterization Analysis of Prevalent Infectious Bronchitis Virus and Pathogenicity Assessment of Recombination Strain in China

Author

Wu, Zhiqiang, primary, Fang, Huanxin, additional, Xu, Zhouyi, additional, Lian, Jiamin, additional, Xie, Zi, additional, Wang, Zhanxin, additional, Qin, Jianpin, additional, Huang, Benli, additional, Feng, Keyu, additional, Zhang, Xinheng, additional, Lin, Wencheng, additional, Li, Hongxin, additional, Chen, Weiguo, additional, and Xie, Qingmei, additional

Published
2022
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39. Recombinant Muscovy Duck Parvovirus Led to Ileac Damage in Muscovy Ducklings

Author

He, Jiahui, primary, Zhang, Yukun, additional, Hu, Zezhong, additional, Zhang, Luxuan, additional, Shao, Guanming, additional, Xie, Zi, additional, Nie, Yu, additional, Li, Wenxue, additional, Li, Yajuan, additional, Chen, Liyi, additional, Huang, Benli, additional, Chu, Fengsheng, additional, Feng, Keyu, additional, Lin, Wencheng, additional, Li, Hongxin, additional, Chen, Weiguo, additional, Zhang, Xinheng, additional, and Xie, Qingmei, additional

Published
2022
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40. Construction and Evaluation of Recombinant Pseudorabies Virus Expressing African Swine Fever Virus Antigen Genes

Author

Chen, Liyi, Zhang, Xinheng, Shao, Guanming, Shao, Yangyang, Hu, Zezhong, Feng, Keyu, Xie, Zi, Li, Hongxin, Chen, Weiguo, Lin, Wencheng, Yuan, Hengxing, Wang, Hailong, Fu, Jun, and Xie, Qingmei

Subjects
General Veterinary
Abstract

African swine fever (ASF) is a highly contact infectious disease caused by the African swine fever virus (ASFV). The extremely complex structure and infection mechanism make it difficult to control the spread of ASFV and develop the vaccine. The ASFV genome is huge with many antigenic genes. Among them, CP204L (p30), CP530R (pp62), E183L (p54), B646L (p72), and EP402R (CD2v) are involved in the process of the virus cycle, with strong immunogenicity and the ability to induce the body to produce neutralizing antibodies. In this study, the recombinant virus rBartha-K61-pASFV that expresses the above ASFV antigen genes was constructed by Red/ET recombineering technology using pseudorabies virus (PRV) vaccine strain Bartha-K61. Western blot analysis showed that the ASFV antigen gene was expressed and the recombinant virus showed good genetic stability and proliferation characteristics in 15 continuous generations on porcine kidney (PK15) cells. The results of immunoassay of piglets and mice showed that rBartha-K61-pASFV had good immunogenicity and could induce higher antibody levels in the body. Therefore, PRV was a promising viral vector for expressing the ASFV antigen gene, and all the experiments in this study laid a foundation for the further development of a new viral vector vaccine of ASFV.

Published
2022

44. Chromosome-level genome assembly of goose provides insight into the adaptation and growth of local goose breeds.

Author

Zhao, Qiqi, Lin, Zhenping, Chen, Junpeng, Xie, Zi, Wang, Jun, Feng, Keyu, Lin, Wencheng, Li, Hongxin, Hu, Zezhong, Chen, Weiguo, Chen, Feng, Junaid, Muhammad, Zhang, Huanmin, Xie, Qingmei, and Zhang, Xinheng

Subjects
GEESE, CHROMOSOMES, GENOME-wide association studies, GENOME size, BREEDING, GENOMICS, GENETIC variation
Abstract

Background Anatidae contains numerous waterfowl species with great economic value, but the genetic diversity basis remains insufficiently investigated. Here, we report a chromosome-level genome assembly of Lion-head goose (Anser cygnoides), a native breed in South China, through the combination of PacBio, Bionano, and Hi-C technologies. Findings The assembly had a total genome size of 1.19 Gb, consisting of 1,859 contigs with an N50 length of 20.59 Mb, generating 40 pseudochromosomes, representing 97.27% of the assembled genome, and identifying 21,208 protein-coding genes. Comparative genomic analysis revealed that geese and ducks diverged approximately 28.42 million years ago, and geese have undergone massive gene family expansion and contraction. To identify genetic markers associated with body weight in different geese breeds, including Wuzong goose, Huangzong goose, Magang goose, and Lion-head goose, a genome-wide association study was performed, yielding an average of 1,520.6 Mb of raw data that detected 44,858 single-mucleotide polymorphisms (SNPs). Genome-wide association study showed that 6 SNPs were significantly associated with body weight and 25 were potentially associated. The significantly associated SNPs were annotated as LDLRAD4, GPR180 , and OR , enriching in growth factor receptor regulation pathways. Conclusions We present the first chromosome-level assembly of the Lion-head goose genome, which will expand the genomic resources of the Anatidae family, providing a basis for adaptation and evolution. Candidate genes significantly associated with different goose breeds may serve to understand the underlying mechanisms of weight differences. [ABSTRACT FROM AUTHOR]

Published
2023
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