17 results on '"Linda B. Giorgi"'
Search Results
2. Redox potentials of cytochrome b-559 in the D1/D2/cytochrome b-559 reaction centre of Photosystem II
- Author
-
David R. Klug, G. Porter, Linda B. Giorgi, Iqbal Ahmad, and James Barber
- Subjects
Photosynthetic reaction centre ,Cytochrome ,biology ,Photosystem II ,Cytochrome b ,Chemistry ,Biophysics ,Cytochrome b559 ,Cell Biology ,Photosynthesis ,Photochemistry ,Biochemistry ,Redox ,Crystallography ,Redox titration ,biology.protein - Abstract
Redox titrations of a stable form of the Photosystem II (PS II) reaction centre, isolated from peas, have detected three redox forms of cytochrome b-559: a high-potential form (+ 430 mV), an intermediate-potential form (+ 180 mV) and a low-potential form (+ 25 mV) with relative amplitudes of 28%, 62% and 10%, respectively. These results contrast with the observations of Shuvalov et al. (Shuvalov, V.A., Heber, U. and Schreiber, U. (1989) FEBS Lett. 258, 27–31), who reported midpoint potentials much lower than these for cytochrome b-559 in isolated PS II reaction centres. Our data show that, if the reaction centre complex is handled appropriately, then cytochrome b-559 can have redox potentials quite close to those found in intact membrane systems.
- Published
- 1993
3. Characterisation of triplet states in isolated Photosystem II reaction centres: Oxygen quenching as a mechanism for photodamage
- Author
-
James R. Durrant, James Barber, G. Porter, Linda B. Giorgi, and David R. Klug
- Subjects
Photosynthetic reaction centre ,Singlet oxygen ,Oscillator strength ,Biophysics ,Analytical chemistry ,chemistry.chemical_element ,Quantum yield ,P680 ,Cell Biology ,Photochemistry ,Biochemistry ,Oxygen ,chemistry.chemical_compound ,chemistry ,Absorption band ,Triplet state - Abstract
Transient absorption spectroscopy has been used to study the isolated D1/D2/cytochrome b-559 reaction centre complex at 4°C. The D1/D2 reaction centre is observed to have an increased susceptibility to photodamage under aerobic conditions. This is attributed to oxygen quenching of a P680 triplet state, which results in the formation of highly oxidising singlet oxygen. This P680 triplet state is observed to have a lifetime of (1.0±0.1) ms under anaerobic conditions, shortening to (33±3) μs in the presence of oxygen. This state, which has a quantum yield of approx. 30%, is identified as residing upon the primary electron donor P680 by the transient bleaching of its reddest absorption band, which peaks at (680.5±0.5) nm. The shape of the P680 triplet-minus-singlet absorption difference spectrum, and particularly the (12±1) nm bandwidth of the red absorption band bleach, indicate that P680 is probably a pair of excitonically coupled chlorophyll molecules, with the P680 triplet state being localised upon one of these chlorophyll molecules. The red absorption band bleached by P680 triplet formation has a peak extinction coefficient of 133000 M−1 · cm−1 and an oscillator strength 1.1-times larger than that of the Qy-band of a chlorophyll a monomer in ether. It is shown that this P680 triplet state is formed primarily by charge recombination from the primary radical pair state at 4°C. A 3% quantum yield of a carotenoid triple state characterised by an absorption peak at 526 nm is also observed. The observed P680 triplet does not appear to be quenched by this carotenoid.
- Published
- 1990
4. Thermodynamic properties of D1/D2/cytochrome b-559 reaction centres investigated by time-resolved fluorescence measurements
- Author
-
Linda B. Giorgi, B. Crystall, James Barber, Paula J. Booth, G. Porter, and David R. Klug
- Subjects
Photosynthetic reaction centre ,Electron transfer ,Chemistry ,Excited state ,Enthalpy ,Biophysics ,Fluorescence spectrometry ,Analytical chemistry ,Quantum yield ,Cell Biology ,Biochemistry ,Fluorescence ,Fluorescence spectroscopy - Abstract
Photosystem II reaction centres have been studied using time-correlated single photon counting to provide time-resolved fluorescence information. Comparative quantum yield measurements suggest that up to 94% of the chlorophyll in these preparations is coupled to the charge-separation pathway. Analysis of time-resolved fluorescence data suggests a free-energy gap between the excited chlorophyll special pair and the primary radical pair of ΔG( P 680 + Ph − - P 680∗) = −0.11 eV at 277 K. Measurements of this free-energy difference between 277 K and 77 K show that between 220 K and 77 K the contributions of entropy and enthalpy are ΔS( P 680 + Ph − - P 680∗) = +3.8 · 10 −4 eV · K −1 and ΔH( P 680 + Ph − - P 680∗) = −5.0 · 10 −3 eV , respectively. Thus the charge transfer reaction is dominated by entropy contributions between 77 K and 220 K, the ratio of entropy to enthalpy at 220 K being 17:1. The relationship between ΔG and temperature is non-linear over the range 220–270 K, which indicates that enthalpy and/or entropy are temperature dependent in this region.
- Published
- 1990
5. Comparison of primary charge separation in the photosystem II reaction center complex isolated from wild-type and D1-130 mutants of the cyanobacterium Synechocystis PCC 6803
- Author
-
David R. Klug, Linda B. Giorgi, D. Melissa Joseph, Stephen A. P. Merry, James R. Durrant, Javier De Las Rivas, G. Porter, James Barber, and Peter J. Nixon
- Subjects
Photosynthetic reaction centre ,Pheophytin ,P700 ,biology ,Photosystem II ,Chemistry ,Stereochemistry ,Spectrum Analysis ,Synechocystis ,Photosynthetic Reaction Center Complex Proteins ,food and beverages ,Primary charge separation ,Photosystem II Protein Complex ,P680 ,Cell Biology ,Photosystem I ,Photochemistry ,biology.organism_classification ,Cyanobacteria ,Biochemistry ,chemistry.chemical_compound ,Kinetics ,Structure-Activity Relationship ,Mutagenesis, Site-Directed ,Molecular Biology - Abstract
We compare primary charge separation in a photosystem II reaction center preparation isolated from a wild-type (WT) control strain of the cyanobacterium Synechocystis sp. PCC 6803 and from two site-directed mutants of Synechocystis in which residue 130 of the D1 polypeptide has been changed from a glutamine to either a glutamate (mutant D1-Gln130Glu), as in higher plant sequences, or a leucine residue (mutant D1-Gln130Leu). The D1-130 residue is thought to be close to the pheophytin electron acceptor. We show that, when P680 is photoselectively excited, the primary radical pair state P680+Ph- is formed with a time constant of 20-30 ps in the WT and both mutants; this time constant is very similar to that observed in Pisum sativum (a higher plant). We also show that a change in the residue at position D1-130 causes a shift in the peak of the pheophytin Qx-band. Nanosecond and picosecond transient absorption measurements indicate that the quantum yield of radical pair formation (phi RP), associated with the 20-30-ps component, is affected by the identify of the D1-130 residue. We find that, for the isolated photosystem II reaction center particle, phi RP higher plant > phi RP D1-Gln130Glu mutant > phi RP WT > phi RP D1-Gln130Leu mutant. Furthermore, the spectroscopic and quantum yield differences we observe between the WT Synechocystis and higher plant photosystem II, seem to be reversed by mutating the D1-130 ligand so that it is the same as in higher plants. This result is consistent with the previously observed natural regulation of quantum yield in Synechococcus PS II by particular changes in the D1 polypeptide amino acid sequence (Clark, A.K., Hurry, V. M., Gustafsson, P. and Oquist, G. (1993) Proc. Natl. Acad. Sci. U.S.A. 90, 11985-11989).
- Published
- 1996
6. Comparison of PS II Primary Photochemistry in Higher Plant, Synechocystis and Synechocystis Mutants
- Author
-
James R. Durrant, James Barber, D. Melissa Joseph, Javier De Las Rivas, Linda B. Giorgi, Peter J. Nixon, George Porter, Steve A. P. Merry, and David R. Klug
- Subjects
Primary (chemistry) ,Biochemistry ,biology ,Chemistry ,Synechocystis ,Mutant ,biology.organism_classification - Published
- 1995
7. A comparison of the photochemical activity of two forms of Photosystem II reaction centre isolated from sugar beet
- Author
-
Linda B. Giorgi, Guillermo Montoya, Paula J. Booth, Javier De Las Rivas, Rafael Picorel, James Barber, David R. Klug, and G. Porter
- Subjects
Photosynthetic reaction centre ,biology ,Photosystem II ,Pheophytins ,Biophysics ,Fluorescence spectrometry ,Primary charge separation ,Electron donor ,Cell Biology ,Photochemistry ,biology.organism_classification ,Biochemistry ,Purple bacteria ,chemistry.chemical_compound ,Electron transfer ,chemistry - Abstract
Both time-resolved fluorescence and absorption measurements have been conducted on two different forms of Photosystem II reaction centre isolated from sugar beet. One form, called RC IIa, contained 6 chlorophylls and 2 β-carotenes per 2 pheophytins, while the other, called RC IIb, contained 4 chlorophylls and 1 β-carotene per 2 pheophytins. Single photon-counting fluorescence decay obtained from the two preparations showed similar charge recombination fluorescence lifetimes which could be resolved into two components of 46.1 and 14.2 ns. Analysis of the amplitude of the fluorescence of the fast component of 5.6 ns, which largely originates from non-functional chlorophyll, gave an estimate of the relative activity for RC IIb which was only a 5.5% lower compared to that of RC IIa. This small relative difference in photochemical activity was also confirmed by measuring the extent of primary charge separation activity using flash induced absorption spectroscopy. In this case the amplitude of the long-lived component, attributed to primary radical-pair formation and recombination, was 16% lower in RC IIb as compared with RC IIa. When the secondary electron transfer activity of the two forms of reaction centre were measured using MnCl2 and silicomolibdate as electron donor and acceptor respectively, RC IIb was 16% less active than RC IIa. From the data we conclude that the removal of 2 chlorophylls and 1 β-carotene molecules from the isolated Photosystem II reaction centre only slightly impair its functional activity with respect to primary charge separation. This conclusion seems to suggest that photochemically active isolated reaction centres of Photosystem II and purple bacteria can have the same minimum pigment stoichiometry of 4 chlorophylls and 1 carotenoid per 2 pheophytins.
- Published
- 1994
8. Characterisation of Triplet and Quinone Induced Cation Radical States in the Isolated Photosystem Two Reaction Centre
- Author
-
James R. Durrant, David R. Klug, James Barber, Linda B. Giorgi, and G. Porter
- Subjects
Electron transfer ,Photosystem II ,Chemistry ,Ultrafast laser spectroscopy ,P680 ,Steady state (chemistry) ,Triplet state ,Photochemistry ,Quinone ,Photosystem - Abstract
Transient absorption spectroscopy has been used to study isolated Photosystem 2 (PS2) reaction centres stabilised by the use of anaerobic conditions. In the absence of added artificial electron donors and acceptors, the light induced electron transfer properties of the reaction centre are restricted to the formation of the radical pair P680+Pheophytin− and charge recombination pathways from this state [1]. This charge recombination has been observed to produce a 23% yield of a chlorophyll triplet state [1]. Attempts to reconstitute these particles with quinone have until now been limited to the observation of a steady state, quinone-mediated photoreduction of the cytochrome b-559 [2].
- Published
- 1990
9. Oxygen Quenching of Triplet States in Isolated Photosystem 2 Reaction Centres: A Mechanism for Photodamage
- Author
-
Linda B. Giorgi, James R. Durrant, B. Crystall, James Barber, David R. Klug, Paula J. Booth, and G. Porter
- Subjects
Reaction centre ,Quenching (fluorescence) ,Cytochrome ,biology ,Photosystem II ,Chemistry ,biology.protein ,chemistry.chemical_element ,Photochemistry ,Oxygen - Abstract
Photosystem 2 (PS2) is involved in the oxidation of water which results in the liberation of oxygen, the mechanism of which is still poorly understood. Over the last few years, studies of PS2 have been greatly advanced by the isolation of the D1/D2/ cytochrome b-559 reaction centre complex (Nanba and Satoh, 1897; Barber et al., 1987). In particular, the lack of an antenna complex associated with this reaction centre has greatly simplified spectroscopic studies, although this reaction centre has been found to degrade rapidly under illumination, which hinders such investigations. In this paper we describe the stabilisation of the sample by the removal of oxygen and suggest a mechanism for photodamage which may be related to the rapid turnover of D1 in vivo.
- Published
- 1990
10. Redox-potentiometric titrations of the electrochromic absorption change in chloroplasts
- Author
-
Nigel K. Packham, James Barber, and Linda B. Giorgi
- Subjects
P700 ,Photosystem II ,Biophysics ,Analytical chemistry ,Plastoquinone ,DCMU ,Cell Biology ,Photosystem I ,Photochemistry ,Biochemistry ,Redox ,Acceptor ,chemistry.chemical_compound ,chemistry ,Photosystem - Abstract
Two phases of the electrochromic 515 nm absorption change in chloroplasts elicited by microsecond flashes can be resolved kinetically. Redox-potentiometric titrations indicate that the initial amplitude appearing within 0.5 ms, and designated as phase a, has three components in the low-potential region with E m7.5 values of +60 mV, −195 mV and less than −400 mV. From the insensitivity to DCMU, we propose that the species with E m7.5 values of −195 mV and less than −400 mV are both related to Photosystem I. This conclusion was supported by the loss of both components when the Photosystem I reaction centre (P-700) was chemically oxidised ( E m7.5 = +370 mV). The species having an E m7.5 less than −400 mV is presumed to be the Photosystem I primary acceptor, while the E m7.5 = −195 mV wave could be due to a secondary electron acceptor, such as cytochrome b -563 LP , whose photoreduction is possible owing to the long duration of the excitation flash. The DCMU-sensitive component with an E m7.5 of +60 mV is assumed to be the primary quinone acceptor (Q A ) of Photosystem II. Unlike the Photosystem I redox components, the midpoint potential of this species is sensitive to the background ionic level: the E m7.5 is shifted to −100 mV when the cation concentration is lowered to facilitate membrane unstacking. The slow phase of the electrochromic signal (phase b) has been estimated by measuring the 2,5-dibromo-3-methyl-6-isopropyl- p -benzoquinone-sensitive amplitude of the absorption change at 20 ms. The signal appears with an estimated E m7.5 = +50 mV, becomes maximal at −50 mV and attenuates with an E m7.5 of about −180 mV. These results suggest that phase b occurs when the plastoquinone pool is reduced and cytochrome b -563 LP is oxidised.
- Published
- 1985
11. The state of detergent solubilised light-harvesting chlorophyll-a/b protein complex as monitored by picosecond time-resolved fluorescence and circular dichroism
- Author
-
Jonathan P. Ide, George Porter, David R. Klug, Werner Kühlbrandt, and Linda B. Giorgi
- Subjects
Circular dichroism ,Quenching (fluorescence) ,Chemistry ,Biophysics ,Fluorescence spectrometry ,food and beverages ,Trimer ,Cell Biology ,Chromophore ,Photochemistry ,Biochemistry ,Fluorescence ,Light-harvesting complex ,Crystallography ,chemistry.chemical_compound ,Chlorophyll - Abstract
Steady-state and picosecond time-resolved fluorescence techniques in conjunction with circular dichroism have been used to study the light-harvesting chlorophyll-a/b protein complex (LHC) isolated from pea chloroplasts. In particular, the effect of changing the detergent / chlorophyll ratio on the state of the LHC has been investigated. Our results have been interpreted in light of the known protein geometry of the LHC in 2-dimensional crystals (Kuhlbrandt, W. (1984) Nature 307, 478–479). The fluorescence lifetime data reveals 1 / e-lifetimes of 3.53 (±0.04) ns and 1.10 (±0.01) ns for a stable, efficiently energy-transferring state of the LHC. Subnanosecond lifetimes are observed under conditions leading to aggregation, while a long component of 5.50 (±0.16) ns corresponding to free Chl a is found when the detergent / chlorophyll ratio is high. The circular dichroism shows a major Chl-b exciton, a Chl-a / b exciton and a further ‘quenching’ Chl-b exciton. These have been attributed to: a C3 symmetric Chl-b interaction for which the intact C3 protein trimer geometry is a prerequisite; a dimeric Chl-a / b interaction, the presence of which is critically dependent on the detergent type; and a further Chl-b interaction which arises from the presence of aggregated trimers, respectively. We have found that the degree of heterogeneity with respect to the oligomeric state of the pigment-protein trimers is dependent upon the detergent / chlorophyll ratio used. Low detergent / chlorophyll ratios result in extensive aggregation of the trimers with a geometry similar to that found in 2-dimensional crystals of the LHC. Moderate detergent conditions yield predominantly non-aggregated trimers. Excess detergent conditions result in considerable chromophore heterogeneity and loss of the main Chl-b exciton consistent with protein denaturation through an initial break up of the trimer geometry. From these results we believe that in vitro the minimum stable functional unit corresponds to a C3 symmetric protein trimer.
- Published
- 1987
12. Redox Titrations of the Fast and Slow Phase of the 515nm Electrochromic Absorption Change in Chloroplasts
- Author
-
Nigel K. Packham, James Barber, and Linda B. Giorgi
- Subjects
Membrane ,Chemistry ,Electrochromism ,Phase (matter) ,Redox titration ,Inorganic chemistry ,Absorption (chemistry) ,Photochemistry ,Electron transport chain ,Redox ,Chloroplast thylakoid - Abstract
The fast phase (phase a) of the 515 nm electrochromic absorption change, ΔA515, occurs as a result of the transmembrane electric field set up at the photochemical reaction centres upon flash activation (Witt 1979). An additional slow increase in the ΔA515 is thought to be due to a subsequent electrogenic step occurring during electron transport (Crowther, Hind 1980). Redox titrations of phase a have shown that both reaction centres contribute to the extent of the electric field (Malkin 1978; Diner, Delosme 1983), however the redox component responsible for phase b is less well characterized. Using chloroplast thylakoid membranes, redox titrations of both phase a and phase b have been carried out in order to investigate the various contributions to phase a under different salt conditions and to determine the component responsible for phase b.
- Published
- 1984
13. Energy transfer to low energy chlorophyll species prior to trapping by P700 and subsequent electron transfer
- Author
-
Linda B. Giorgi, G. Porter, David R. Klug, James Barber, and B. Crystall
- Subjects
Physics::Biological Physics ,Quenching (fluorescence) ,P700 ,Chemistry ,P680 ,Cell Biology ,Plant Science ,General Medicine ,Photosystem I ,Biochemistry ,Electron transfer ,chemistry.chemical_compound ,Chemical physics ,Chlorophyll ,Metastability ,Singlet state ,Atomic physics - Abstract
It is found that the two singlet state lifetimes observed in medium sized isolated Photosystem One reaction centres belong to two distinct sets of particles. The nanosecond lifetime is due to PS1 particles in which P700 does not trap excitation energy, and the excitation energy is homogeneously distributed within the antennae of these particles. The spectral features of the picosecond component show that excitation energy in the antenna has become largely concentrated in one or more low energy (red) chlorophyll species within 3.5 ps. Antennae which have become decoupled from P700 also appear to be decoupled from these red “ancillary” chlorophylls, and this suggests that some substructure or level of organisation links them to P700. The rate of quenching of antenna singlet states appears to be independent of the redox state of P700 under the conditions used here, and oxidising P700 does not prevent excitation energy from reaching the red chlorophyll species in the antenna. We find no evidence in the data presented here of a chlorophyll molecule acting as a “metastable” primary acceptor (A0). The lower limit for the detection of such a species in these data is 20% of the optical density of the transient P700 bleach.
- Published
- 1989
14. Picosecond absorption spectroscopy of Photosystem I reaction centres from higher plants
- Author
-
Bryson L. Gore, James Barber, David R. Klug, Tom Doust, Linda B. Giorgi, and George Porter
- Subjects
Absorption spectroscopy ,Chemistry ,Picosecond ,Photochemistry ,Photosystem I ,Biochemistry - Published
- 1986
15. Picosecond fluorescence and absorption spectroscopy of light-harvesting chlorophyll-protein complex from pea chloroplasts
- Author
-
Linda B. Giorgi, Tom Doust, P. Ide Jonathan, James Barber, Werner Kühlbrandt, Bryson L. Gore, George Porter, and David R. Klug
- Subjects
Chloroplast ,Absorption spectroscopy ,Chemistry ,Picosecond ,Light-Harvesting Chlorophyll Protein ,Photochemistry ,Biochemistry ,Fluorescence - Published
- 1986
16. The design of a picosecond flash spectroscope and its application to photosynthesis
- Author
-
Bryson L. Gore, Linda B. Giorgi, B. Crystall, Tom Doust, David R. Klug, Jonathan P. Ide, and George Porter
- Subjects
Flash (photography) ,Absorption spectroscopy ,Spectrometer ,Chemistry ,Picosecond ,Analytical chemistry ,Photosynthesis ,Photosystem I - Abstract
The design and characteristics of the current picosecond flash-photolysis apparatus at the Royal Institution is described. This equipment is being applied to the investigation of various photosynthetic materials isolated from higher plants. Typical absorption spectra obtained with Photosystem 1 (PS1) reaction centres, isolated from pea chloroplast, are presented to show the quality of data now obtainable on a picosecond timescale.
- Published
- 1986
17. Detergent effects upon the picosecond dynamics of higher-plant light-harvesting chlorophyll complex (LHC2)
- Author
-
Jonathan P. Ide, George Porter, James Barber, Linda B. Giorgi, B. Crystall, David R. Klug, Bryson L. Gore, and Werner Kühlbrandt
- Subjects
chemistry.chemical_compound ,Absorption spectroscopy ,chemistry ,Exciton ,Picosecond ,Chlorophyll ,Thylakoid ,Resolution (electron density) ,Analytical chemistry ,food and beverages ,Photochemistry ,Fluorescence ,Fluorescence spectroscopy - Abstract
Resolubilisation of detergent-extracted higher-plant light-harvesting complex (LHC2) from pea thylakoid membranes is shown to produce a sample which is heterogeneous in terms of its aggregation state. The dependence of the degree of aggregation upon the chlorophyll/detergent molar ratio is monitored using the techniques of single-photon counting, circular dichronic absorption spectroscopy and fluorescence spectroscopy. In general, greater aggregation is shown to lead to shorter fluorescence lifetimes and extra exciton features which can be compared with the known symmetry of the crystallised LHC2 previously determined to a resolution of 16 A.
- Published
- 1986
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.