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39. Proton-Induced and Electron-Induced X-Ray Microanalysis of Insulin-Secreting Cells

Author

Pålsgård, Eva, Lindh, Ulf, Juntti-Berggren, Lisa, Berggren, Per-Olof, Roomans, Godfried M., and Grime, Geoffrey W.

Subjects
cell culture, insulin-secreting cells, calcium, electron probe, proton probe, strontium, Biology
Abstract

Elemental redistribution induced by insulin secretion, was investigated by electron and proton probe X-ray microanalysis. In particular, ion fluxes following immediately upon stimulation were studied. As the sensitivity of the electron probe was insufficient, the proton microprobe was employed. In order to see whether the cell is asymmetric with respect to Ca2+ influx, the cells were stimulated in the presence of Sr2+ (as a Ca2+ analog). Insulin-secreting cells (RINm5F cells and isolated mouse β-cells) were cultured on grids and shock-frozen at 2-30 seconds after stimulation. In a large number of cells, the major elements and and large fluxes were analyzed by the electron microprobe. In the proton microprobe, selected cells were analyzed and elemental maps were compared with electron micrographs of the same cells. The proton microprobe, but not the electron microprobe, could detect an influx of Sr in response to K+-stimulation for 2 seconds, in RINm5F-cells. No polarization of Sr2+ uptake in RINm5F-cells could be detected, and the β-cells did not respond to high K+ by uptake of Sr. Momentary stimulation of β-cells also resulted in a significant increase in Na, detected by the electron probe. Spreading of the β-cells on the substrate appears to influence the subcellular elemental distribution. Thus, the proton probe has potential to detect small changes in elements such as those occurring after short-time stimulation.

Published
1994

45. CHAPTER 5: UPTAKE OF ELEMENTS FROM A BIOLOGICAL POINT OF VIEW.

Author

LINDH, ULF

Abstract

Chapter 5 of the book "Essentials of Medical Geology: Impacts of the Natural Environment on Public Health" is presented. It discusses the essentiality of elements and the general aspects of element uptake. It highlights the thermodynamics of transport. It explores the uptake and regulation of iron, zinc and copper.

Published
2005

46. CHAPTER 6: BIOLOGICAL FUNCTIONS OF THE ELEMENTS.

Author

LINDH, ULF

Abstract

Chapter 6 of the book "Essentials of Medical Geology: Impacts of the Natural Environment on Public Health" is presented. It discusses the essentiality of chemical elements. It highlights major, minor and trace elements in biology. It offers a brief description of the function of major and minor chemical elements. It also explores functional value of trace elements.

Published
2005

47. PREFACE.

Author

Selinus, Olle, Alloway, Brian, Centeno, José A., Finkelman, Robert B., Fuge, Ron, Lindh, Ulf, and Smedley, Pauline

Abstract

A preface for the January 2005 issue of the periodical "Essentials of Medical Geology: Impacts of the Natural Environment on Public Health" is presented.

Published
2005

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