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The genetic basis of adaptation to different environments has been of long-standing interest to evolutionary biologists. Dormancy is a well-studied adaptation to facilitate overwintering. In Drosophila melanogaster, a moderate number of genes with large effects have been described, which suggests a simple genetic basis of dormancy. On the other hand, genome-wide scans for dormancy suggest a polygenic architecture in insects. In D. melanogaster, the analysis of the genetic architecture of dormancy is complicated by the presence of cosmopolitan inversions. Here, we performed a genome-wide scan to characterize the genetic basis of this ecologically extremely important trait in the sibling species of D. melanogaster, D. simulans that lacks cosmopolitan inversions. We performed Pool-GWAS in a South African D. simulans population for dormancy incidence at 2 temperature regimes (10 and 12°C, LD 10:14). We identified several genes with SNPs that showed a significant association with dormancy (P-value < 1e-13), but the overall modest response suggests that dormancy is a polygenic trait with many loci of small effect. Our results shed light on controversies on reproductive dormancy in Drosophila and have important implications for the characterization of the genetic basis of this trait., (© The Author(s) 2022. Published by Oxford University Press on behalf of Genetics Society of America.)

The pervasive occurrence of sexual dimorphism demonstrates different adaptive strategies of males and females. While different reproductive strategies of the two sexes are well-characterized, very little is known about differential functional requirements of males and females in their natural habitats. Here, we study the impact environmental change on the selection response in both sexes. Exposing replicated Drosophila populations to a novel temperature regime, we demonstrate sex-specific changes in gene expression, metabolic and behavioral phenotypes in less than 100 generations. This indicates not only different functional requirements of both sexes in the new environment but also rapid sex-specific adaptation. Supported by computer simulations we propose that altered sex-biased gene regulation from standing genetic variation, rather than new mutations, is the driver of rapid sex-specific adaptation. Our discovery of environmentally driven divergent functional requirements of males and females has important implications-possibly even for gender aware medical treatments., Competing Interests: SH, AJ, VN, ML, RK, NB, EV, CS No competing interests declared, (© 2020, Hsu et al.)

Organisms regularly encounter unfavorable conditions and the genetic adaptations facilitating survival have been of long-standing interest to evolutionary biologists. Winter is one particularly stressful condition for insects, during which they encounter low temperatures and scarcity of food. Despite dormancy being a well-studied adaptation to facilitate overwintering, there is still considerable controversy about the distribution of dormancy among natural populations and between species in Drosophila. The current definition of dormancy as developmental arrest of oogenesis at the previtellogenic stage (stage 7) distinguishes dormancy from general stress related block of oogenesis at early vitellogenic stages (stages 8 - 9). In an attempt to resolve this, we scrutinized reproductive dormancy in D. melanogaster and D. simulans. We show that dormancy shows the same hallmarks of arrest of oogenesis at stage 9, as described for other stressors and propose a new classification for dormancy. Applying this modified classification, we show that both species express dormancy in cosmopolitan and African populations, further supporting that dormancy uses an ancestral pathway induced by environmental stress. While we found significant differences between individuals and the two Drosophila species in their sensitivity to cold temperature stress, we also noted that extreme temperature stress (8 °C) resulted in very strong dormancy incidence, which strongly reduced the differences seen at less extreme temperatures. We conclude that dormancy in Drosophila should not be considered a special trait, but is better understood as a generic stress response occurring at low temperatures., (Copyright © 2018 The Authors. Published by Elsevier Ltd.. All rights reserved.)

The Asian tiger mosquito, Aedes albopictus, is a highly successful invasive species that transmits a number of human viral diseases, including dengue and Chikungunya fevers. This species has a large genome with significant population-based size variation. The complete genome sequence was determined for the Foshan strain, an established laboratory colony derived from wild mosquitoes from southeastern China, a region within the historical range of the origin of the species. The genome comprises 1,967 Mb, the largest mosquito genome sequenced to date, and its size results principally from an abundance of repetitive DNA classes. In addition, expansions of the numbers of members in gene families involved in insecticide-resistance mechanisms, diapause, sex determination, immunity, and olfaction also contribute to the larger size. Portions of integrated flavivirus-like genomes support a shared evolutionary history of association of these viruses with their vector. The large genome repertory may contribute to the adaptability and success of Ae. albopictus as an invasive species.

Variation in vectorial capacity for human malaria among Anopheles mosquito species is determined by many factors, including behavior, immunity, and life history. To investigate the genomic basis of vectorial capacity and explore new avenues for vector control, we sequenced the genomes of 16 anopheline mosquito species from diverse locations spanning ~100 million years of evolution. Comparative analyses show faster rates of gene gain and loss, elevated gene shuffling on the X chromosome, and more intron losses, relative to Drosophila. Some determinants of vectorial capacity, such as chemosensory genes, do not show elevated turnover but instead diversify through protein-sequence changes. This dynamism of anopheline genes and genomes may contribute to their flexible capacity to take advantage of new ecological niches, including adapting to humans as primary hosts., (Copyright © 2015, American Association for the Advancement of Science.)

Background: Dibenzoylhydrazine (DBH) compounds have been applied successfully as environmentally safe insecticides against lepidopteran larvae and ground-dwelling coleopterans, but their potential to combat mosquito larvae is largely unknown. Here, toxicity tests of three commercial DBHs (tebufenozide, methoxyfenozide and halofenozide) and one experimental DBH (KU-106) against larvae of Anopheles gambiae, the major vector for human malaria, are reported., Results: Based on calculated median larvicidal concentration (LC50 ) values at 5 days of treatment, KU-106 (760 nM) showed an activity against Anopheles larvae similar to that of commercial halofenozide. Induction of the early-late gene hr3 and docking studies of DBHs in the ligand-binding pocket of the modelled Anopheles ecdysone receptor indicated that toxicity is caused by the activation of the ecdysone regulatory cascade causing a premature lethal moult., Conclusions: As a result of the similar toxicity exhibited by the experimental compound KU-106 to that shown by commercial products, the present study demonstrated that the use of DBH compounds to combat harmful dipteran insects, such as mosquitoes, remains unexplored and invites further systematic toxicity tests using other derivatives of the DBH class of compounds., (© 2012 Society of Chemical Industry.)