13 results on '"Lisette Mbuyi Kalonji"'
Search Results
2. A genomic appraisal of invasive Salmonella Typhimurium and associated antibiotic resistance in sub-Saharan Africa
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Sandra Van Puyvelde, Tessa de Block, Sushmita Sridhar, Matt Bawn, Robert A. Kingsley, Brecht Ingelbeen, Mathew A. Beale, Barbara Barbé, Hyon Jin Jeon, Lisette Mbuyi-Kalonji, Marie-France Phoba, Dadi Falay, Delphine Martiny, Olivier Vandenberg, Dissou Affolabi, Jean Pierre Rutanga, Pieter-Jan Ceyssens, Wesley Mattheus, Wim L. Cuypers, Marianne A. B. van der Sande, Se Eun Park, Simon Kariuki, Kephas Otieno, John P. A. Lusingu, Joyce R. Mbwana, Samuel Adjei, Anima Sarfo, Seth O. Agyei, Kwaku P. Asante, Walter Otieno, Lucas Otieno, Marc C. Tahita, Palpouguini Lompo, Irving F. Hoffman, Tisungane Mvalo, Chisomo Msefula, Fatimah Hassan-Hanga, Stephen Obaro, Grant Mackenzie, Stijn Deborggraeve, Nicholas Feasey, Florian Marks, Calman A. MacLennan, Nicholas R. Thomson, Jan Jacobs, Gordon Dougan, Samuel Kariuki, and Octavie Lunguya
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Science - Abstract
Abstract Invasive non-typhoidal Salmonella (iNTS) disease manifesting as bloodstream infection with high mortality is responsible for a huge public health burden in sub-Saharan Africa. Salmonella enterica serovar Typhimurium (S. Typhimurium) is the main cause of iNTS disease in Africa. By analysing whole genome sequence data from 1303 S. Typhimurium isolates originating from 19 African countries and isolated between 1979 and 2017, here we show a thorough scaled appraisal of the population structure of iNTS disease caused by S. Typhimurium across many of Africa’s most impacted countries. At least six invasive S. Typhimurium clades have already emerged, with ST313 lineage 2 or ST313-L2 driving the current pandemic. ST313-L2 likely emerged in the Democratic Republic of Congo around 1980 and further spread in the mid 1990s. We observed plasmid-borne as well as chromosomally encoded fluoroquinolone resistance underlying emergences of extensive-drug and pan-drug resistance. Our work provides an overview of the evolution of invasive S. Typhimurium disease, and can be exploited to target control measures.
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- 2023
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3. Invasive non-typhoidal Salmonella from stool samples of healthy human carriers are genetically similar to blood culture isolates: a report from the Democratic Republic of the Congo
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Lisette Mbuyi-Kalonji, Liselotte Hardy, Jules Mbuyamba, Marie-France Phoba, Gaëlle Nkoji, Wesley Mattheus, Justin Im, Florian Marks, Hyon Jin Jeon, Jan Jacobs, and Octavie Lunguya
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Salmonella Typhimurium ST313 ,Salmonella Enteritidis ST11 ,human carriers ,reservoir ,Africa ,DR Congo ,Microbiology ,QR1-502 - Abstract
Invasive non-typhoidal Salmonella (iNTS) (serotypes Typhimurium and Enteritidis) are major causes of bloodstream infections in sub-Saharan Africa, but their reservoir is unknown. Aiming to demonstrate human carriers as a reservoir, we assessed an iNTS disease endemic rural community (Kikonka health area, Democratic Republic of the Congo) for intestinal carriage of iNTS. After a census, healthy subjects from randomly selected households provided three successive stool samples for Salmonella culture. We next compared the stool isolates for genetic relatedness with time and health area-matched blood culture isolates obtained from hospitalized patients by multiple locus variable-number tandem repeat analysis (MLVA) and performed whole genome sequencing (WGS) on a subset of stool and blood isolates. Among 2,354 eligible subjects, 2,234 (94.9%) consented and provided at least one stool sample, and 2,219 (94.3%) provided three stool samples. The cumulative proportion of Salmonella carriers after 3 days was 4.4% (n = 98). S. Typhimurium and Enteritidis were found in 26 and 3 carriers, respectively, representing 1.3% (29 out of 2,234) of participants living in 6.0% (26 out of 482) of households. MLVA types of all 26 S. Typhimurium stool isolates matched with the corresponding MLVA types of blood isolates. The MLVA type of one out of three Enteritidis stool isolates matched the single MLVA type of the five Enteritidis blood isolates. WGS analysis of S. Typhimurium (n = 20) and S. Enteritidis (n = 4) isolates revealed Typhimurium multilocus sequence type (ST)313 Lineage 2 and Enteritidis ST11 Central/Eastern African and Outlier clades and confirmed the MLVA clustering. More than three-quarters of Typhimurium isolates showed combined multidrug resistance, ceftriaxone resistance, and fluoroquinolone non-susceptibility. In conclusion, the present study demonstrated iNTS carriage among healthy community members, with stool isolates that were genetically similar to blood culture isolates obtained in patients from the same community. These findings contribute to the evidence of a human reservoir of iNTS.
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- 2023
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4. Non-typhoidal Salmonella intestinal carriage in a Schistosoma mansoni endemic community in a rural area of the Democratic Republic of Congo.
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Lisette Mbuyi-Kalonji, Barbara Barbé, Gaëlle Nkoji, Joule Madinga, Clémentine Roucher, Sylvie Linsuke, Marie Hermy, Anne-Sophie Heroes, Wesley Mattheus, Katja Polman, Pascal Lutumba, Marie-France Phoba, Octavie Lunguya, and Jan Jacobs
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Arctic medicine. Tropical medicine ,RC955-962 ,Public aspects of medicine ,RA1-1270 - Abstract
BACKGROUND:Clinical observations and animal studies have suggested that Salmonella intestinal carriage is promoted by concurrent Schistosoma infection. The present study assessed association of Salmonella intestinal carriage and Schistosoma mansoni infection among individuals in a Schistosoma endemic area in sub-Saharan Africa. METHODS:From November 2015 to March 2016, a cross-sectional community-wide study was conducted in Kifua II, a rural village in Kongo Central Province, Democratic Republic of Congo. Stool samples were collected and analyzed for Salmonella intestinal carriage (culture) and Schistosoma mansoni infection (Kato Katz microscopy with determination of egg load). Salmonella Typhimurium and Enteritidis isolates were assessed for genetic similarity with blood culture isolates obtained during the same period in a neighboring hospital using multi-locus variable-numbers tandem repeat analysis (MLVA). RESULTS:A total of 1,108 participants were included (median age 15 years (IQR: 7-36), male-to-female ratio of 1:1.1). The overall prevalence of Schistosoma mansoni infection and non-typhoidal Salmonella carriage was 51.2% (95% CI: 48.2-54.1) and 3.4% (95% CI: 2.5-4.7) respectively, with 2.2% (95% CI: 1.5-3.2) of participants coinfected. The proportion of Salmonella carriage tended to be higher among Schistosoma mansoni infected participants compared to non-infected participants but this difference did not reach statistical significance (4.2% versus 2.6%, p = 0.132). However, the proportion of Salmonella carriage among participants with a heavy Schistosoma mansoni infection was significantly higher compared to those with a light and moderate infection (8.7% versus 3.2%, p = 0.012) and compared to Schistosoma mansoni negatives (8.7% versus 2.6%, p = 0.002). The 38 Salmonella isolates comprised five and four Enteritidis and Typhimurium serotypes respectively, the majority of them had MLVA types identical or similar to those observed among blood culture isolates. CONCLUSION:Salmonella intestinal carriage was associated with a heavy intensity of Schistosoma mansoni infection. Further studies are needed to address causation.
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- 2020
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5. Direct association between rainfall and non-typhoidal Salmonella bloodstream infections in hospital-admitted children in the Democratic Republic of Congo
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Jan Jacobs, Bieke Tack, Liesbet Jacobs, Marie-France Phoba, Barbara Barbé, Lisette Mbuyi-Kalonji, Liselotte Hardy, Daniel Vita, Octavie Lunguya, and Ecosystem and Landscape Dynamics (IBED, FNWI)
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Male ,Salmonella typhimurium ,Epidemiology ,Non typhoidal salmonella ,DISEASE ,Environmental impact ,Risk Factors ,Case fatality rate ,CLIMATE-CHANGE ,Multidisciplinary ,Under-five ,Transmission (medicine) ,respiratory system ,Anti-Bacterial Agents ,Multidisciplinary Sciences ,Hospitalization ,Child, Preschool ,Salmonella Infections ,Democratic Republic of the Congo ,Science & Technology - Other Topics ,Infectious diseases ,Medicine ,Female ,Seasons ,Wet season ,BACTEREMIA ,Anemia ,Science ,Biology ,TIME-SERIES REGRESSION ,Paediatric research ,Article ,REGION ,Environmental health ,Sepsis ,SURVEILLANCE ,MANAGEMENT ,medicine ,Humans ,Clinical microbiology ,Retrospective Studies ,Science & Technology ,CLINICAL PRESENTATION ,Infant, Newborn ,Infant ,Bacteriology ,ENTERICA ,medicine.disease ,Malnutrition ,Salmonella enteritidis ,nervous system ,RESISTANCE ,Malaria - Abstract
Non-typhoidal Salmonella (NTS) ranks first among causes of bloodstream infection in children under five years old in the Democratic Republic of Congo and has a case fatality rate of 15%. Main host-associated risk factors are Plasmodium falciparum malaria, anemia and malnutrition. NTS transmission in sub-Saharan Africa is poorly understood. NTS bloodstream infections mostly occur during the rainy season, which may reflect seasonal variation in either environmental transmission or host susceptibility. We hypothesized that environment- and host-associated factors contribute independently to the seasonal variation in NTS bloodstream infections in children under five years old admitted to Kisantu referral hospital in 2013–2019. We used remotely sensed rainfall and temperature data as proxies for environmental factors and hospital data for host-associated factors. We used principal component analysis to disentangle the interrelated environment- and host-associated factors. With timeseries regression, we demonstrated a direct association between rainfall and NTS variation, independent of host-associated factors. While the latter explained 17.5% of NTS variation, rainfall explained an additional 9%. The direct association with rainfall points to environmental NTS transmission, which should be explored by environmental sampling studies. Environmental and climate change may increase NTS transmission directly or via host susceptibility, which highlights the importance of preventive public health interventions.
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- 2021
6. Epidemiological cut-off value and antibiotic susceptibility test methods for azithromycin in a collection of multi-country invasive non-typhoidal Salmonella
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Bieke Tack, Marie-France Phoba, Phe Thong, Palpouguini Lompo, Charlien Hupko, Stefanie Desmet, Delphine Martiny, Wesley Mattheus, Maria Pardos de la Gandara, Lisette Mbuyi-Kalonji, Laura Kuijpers, Benoit Prevost, Barbara Barbé, Olivier Vandenberg, Octavie Lunguya, Joaquim Ruiz, Jan Jacobs, and Liselotte Hardy
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Microbiology (medical) ,Agar ,Infectious Diseases ,Salmonella ,Salmonella Infections ,Humans ,General Medicine ,Microbial Sensitivity Tests ,Azithromycin ,Typhoid Fever ,Anti-Bacterial Agents - Abstract
OBJECTIVE: Azithromycin is an alternative to treat invasive non-typhoidal Salmonella (iNTS) infections. We determined its epidemiological cut-off (ECOFF) and compared azithromycin susceptibility testing methods for iNTS. METHODS: We used EUCAST ECOFFinder to determine the minimum inhibitory concentrations (MIC; obtained by broth microdilution) ECOFF and corresponding disk zone diameters of 515 iNTS from blood cultures in Democratic Republic of Congo, Burkina Faso, Rwanda, and Cambodia. Transferable resistance mechanisms were determined by polymerase chain reaction. We compared azithromycin susceptibility testing by semi-automated broth microdilution (customized Sensititre panel; reference), agar dilution, gradient tests (bioMérieux, Liofilchem, HiMedia; read at 80% (MIC80%) and 100% inhibition (MIC100%)), and disk diffusion (Rosco, Oxoid, BD, Liofilchem) for 161 wild- and 198 non-wild-type iNTS. RESULTS: Azithromycin MIC ECOFF was 16 mg/L corresponding to a 12 mm zone diameter; mphA was detected in 192/197 non-wild- and 0/47 wild-type iNTS. Categorical agreement was excellent (≥98%) for all methods. Essential agreement was very good for agar dilution (>90%) but moderate for gradient tests (MIC80%: 52% to 71% and MIC100%: 72% to 91%). Repeatability was good for all methods/brands. Interreader agreement was high for broth microdilution and agar dilution (all ≤1 twofold dilution difference) and disk diffusion (>96% ≤3 mm difference) but lower for gradient tests (MIC80% & MIC100%: 83% to 94% ≤1 twofold dilution difference). DISCUSSION: Azithromycin ECOFF of iNTS was 16 mg/L, i.e. equal to Salmonella Typhi. Disk diffusion is an accurate, precise, and user-friendly alternative for agar dilution and broth microdilution. Reading gradient tests at 100% instead of 80% inhibition improved accuracy and precision. ispartof: CLINICAL MICROBIOLOGY AND INFECTION vol:28 issue:12 pages:1615-1623 ispartof: location:England status: published
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- 2022
7. The Severe Typhoid in Africa Program: Incidences Of Typhoid Fever in Burkina Faso, Democratic Republic of Congo, Ethiopia, Ghana, Madagascar, And Nigeria
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Florian Marks, Justin Im, Se Eun Park, Gideok Pak, Hyonjin Jeon, Lady Rosny Wandji Nana, Marie-France Phoba, Lisette Mbuyi-Kalonji, Ondari D. Mogeni, Biruk Yeshitela, Ursula Panzner, Ligia Maria Cruz Espinoza, Tigist Beyene, Michael Owusu Ansah, Sampson Twumasi-Ankrah, Melese Yeshambaw, Ashenafi Alemu, Oluwafemi Adewusi, Olukemi Adekanmbi, Ellen Higginson, Akinlolu Adepoju, Sarah Agbi, Enoch G. Cakpo, Veronica O. Ogunleye, Gaëlle Nkoji, Odion O. Ikhimiukor, Jules Mbuyamba, Trevor Toy, Francis Opoku Agyapong, Tsiriniaina Jean Luco Razafindrabe, Tiana Mirana Raminosoa, Gabriel Nyrienda, Hyeong Won Seo, Hyejin Seo, Mohamadou Siribie, Megan E. Carey, Michael Owusu, Christian G. Meyer, Ndrainaharimira Rakotozandrindrainy, Nimarko Sarpong, Mathilde Razafindrakalia, Ravomialisoa Razafimanantsoa, Moussa Ouedraogo, Yeonseon J. Kim, Raphaël M. Zellweger, Sophie SY Kang, Juyeon Park, John A. Crump, Liselotte Hardy, Jan Jacobs, Denise Garrett, Jason Andrews, Nimesh Poudyal, Deok Ryun Kim, John D. Clemens, Stephen G. Baker, Jerome H. Kim, Gordon Dougan, Jonathan D. Sugimoto, Sandra Van Puyvelde, Aderemi Kehinde, Oluwafemi A. Popoola, Vittal Mogasale, Robert Breiman, William MacWright, Abraham Aseffa, Birkneh Tilahun Tadesse, Andrea Haselbeck, Yaw Adu-Sarkodie, Mekonnen Teferi, Abdramane Soura Bassiahi, Iruka Okeke, Octavie Lunguya-Metila, Ellis Owusu-Dabo, and Raphaël Rakotozandrindrainy
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History ,Polymers and Plastics ,Business and International Management ,Industrial and Manufacturing Engineering - Published
- 2022
8. Non-typhoidal Salmonella bloodstream infections in Kisantu, DR Congo: Emergence of O5-negative Salmonella Typhimurium and extensive drug resistance
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Liselotte Hardy, Barbara Barbé, Jan Jacobs, Bieke Tack, Stijn Deborggraeve, Dauly Ngbonda, Marie-France Phoba, Marianne A B van der Sande, Octavie Lunguya, Lisette Mbuyi Kalonji, Dadi Falay, Sandra Van Puyvelde, and Brecht Ingelbeen
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0301 basic medicine ,Bacterial Diseases ,Male ,Salmonella typhimurium ,Salmonella ,Physiology ,RC955-962 ,Bacteremia ,Drug resistance ,Azithromycin ,medicine.disease_cause ,Pathology and Laboratory Medicine ,0302 clinical medicine ,Antibiotics ,Ciprofloxacin ,Drug Resistance, Multiple, Bacterial ,Arctic medicine. Tropical medicine ,Medicine and Health Sciences ,Medicine ,Child ,Antimicrobials ,Ceftriaxone ,Drugs ,Hematology ,Bacterial Pathogens ,Body Fluids ,Anti-Bacterial Agents ,Infectious Diseases ,Blood ,Congo ,Medical Microbiology ,Child, Preschool ,Salmonella Infections ,Female ,Pathogens ,Anatomy ,Public aspects of medicine ,RA1-1270 ,medicine.drug ,Research Article ,Adult ,Adolescent ,Salmonella enteritidis ,030231 tropical medicine ,Microbial Sensitivity Tests ,Serogroup ,Microbiology ,Antibiotic Susceptibility Testing ,03 medical and health sciences ,Young Adult ,Antibiotic resistance ,Enterobacteriaceae ,Microbial Control ,Humans ,Typhoid Fever ,Microbial Pathogens ,Pharmacology ,Bacteria ,business.industry ,Public Health, Environmental and Occupational Health ,Organisms ,Infant, Newborn ,Biology and Life Sciences ,Infant ,Bloodstream Infections ,bacterial infections and mycoses ,Multiple drug resistance ,030104 developmental biology ,Pharmacologic Analysis ,Antibiotic Resistance ,Human medicine ,Antimicrobial Resistance ,business ,human activities - Abstract
Background Non-typhoidal Salmonella (NTS) are a major cause of bloodstream infection (BSI) in sub-Saharan Africa. This study aimed to assess its longitudinal evolution as cause of BSI, its serotype distribution and its antibiotic resistance pattern in Kisantu, DR Congo. Methods As part of a national surveillance network, blood cultures were sampled in patients with suspected BSI admitted to Kisantu referral hospital from 2015–2017. Blood cultures were worked-up according to international standards. Results were compared to similar data from 2007 onwards. Results In 2015–2017, NTS (n = 896) represented the primary cause of BSI. NTS were isolated from 7.6% of 11,764 suspected and 65.4% of 1371 confirmed BSI. In children, Author summary We reviewed bloodstream infections caused by non-typhoidal Salmonella over a 10 year period (2007–2017) in the Democratic Republic of the Congo, with Kisantu in the Kongo Central province as the major sampling site. Non-typhoidal Salmonella were the most common cause of bloodstream infections in children in a sentinel network in DR Congo. The disease mainly occurred in young children and had a high mortality. We showed that non-typhoidal Salmonella persisted to frequently cause bloodstream infections in children under 5 years old. Salmonella Enteritidis and Salmonella Typhimurium were the main serotypes causing non-typhoidal Salmonella bloodstream infections. The emergence of O5-antigen negative Salmonella Typhimurium (i.e. variant Copenhagen) was observed and this needs to be considered when developing vaccines. Multidrug resistance to ampicillin, trimethoprim-sulfamethoxazole and chloramphenicol was widespread during the whole decade. From 2013 onwards, resistance to all other available antibiotics, i.e. third generation cephalosporins, fluoroquinolones and azithromycin, emerged. Likewise, extensive drug resistance (multidrug, ceftriaxone and azithromycin co-resistance) and multidrug resistance combined with decreased ciprofloxacin susceptibility in Salmonella Typhimurium emerged. Unfortunately, there are no clinical studies on antibiotic treatment in non-typhoidal Salmonella bloodstream infection that could allow the development of evidence based treatment guidelines. Therefore, dedicated observational studies and clinical studies are urgently needed to optimize antibiotic stewardship.
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- 2020
9. Non-typhoidal Salmonella intestinal carriage in a Schistosoma mansoni endemic community in a rural area of the Democratic Republic of Congo
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Octavie Lunguya, Gaëlle Nkoji, Marie-France Phoba, Pascal Lutumba, Barbara Barbé, Joule Madinga, Lisette Mbuyi-Kalonji, Katja Polman, Anne-Sophie Heroes, Jan Jacobs, Wesley Mattheus, Clémentine Roucher, Sylvie Linsuke, Marie Hermy, and Infectious Diseases
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Bacterial Diseases ,Male ,Rural Population ,Salmonella typhimurium ,0301 basic medicine ,Serotype ,Salmonella ,Veterinary medicine ,Salmonellosis ,Schistosoma Mansoni ,Physiology ,RC955-962 ,Fevers ,Pathology and Laboratory Medicine ,Salmonella typhi ,medicine.disease_cause ,Salmonella Typhi ,0302 clinical medicine ,Arctic medicine. Tropical medicine ,Medicine and Health Sciences ,Blood culture ,Child ,biology ,medicine.diagnostic_test ,Coinfection ,Eukaryota ,Bacterial Pathogens ,Body Fluids ,Intestines ,Blood ,Infectious Diseases ,Medical Microbiology ,Carrier State ,Democratic Republic of the Congo ,Schistosoma ,Female ,Schistosoma mansoni ,Public aspects of medicine ,RA1-1270 ,Pathogens ,Anatomy ,Research Article ,Adult ,Adolescent ,030231 tropical medicine ,Multiple Loci VNTR Analysis ,Microbiology ,Young Adult ,03 medical and health sciences ,Signs and Symptoms ,Enterobacteriaceae ,Diagnostic Medicine ,Helminths ,parasitic diseases ,medicine ,Animals ,Humans ,Microbial Pathogens ,Bacteria ,Organisms ,Public Health, Environmental and Occupational Health ,Biology and Life Sciences ,biology.organism_classification ,Invertebrates ,Schistosomiasis mansoni ,Gastrointestinal Tract ,Cross-Sectional Studies ,030104 developmental biology ,Carriage ,Digestive System - Abstract
Background Clinical observations and animal studies have suggested that Salmonella intestinal carriage is promoted by concurrent Schistosoma infection. The present study assessed association of Salmonella intestinal carriage and Schistosoma mansoni infection among individuals in a Schistosoma endemic area in sub-Saharan Africa. Methods From November 2015 to March 2016, a cross-sectional community-wide study was conducted in Kifua II, a rural village in Kongo Central Province, Democratic Republic of Congo. Stool samples were collected and analyzed for Salmonella intestinal carriage (culture) and Schistosoma mansoni infection (Kato Katz microscopy with determination of egg load). Salmonella Typhimurium and Enteritidis isolates were assessed for genetic similarity with blood culture isolates obtained during the same period in a neighboring hospital using multi-locus variable-numbers tandem repeat analysis (MLVA). Results A total of 1,108 participants were included (median age 15 years (IQR: 7–36), male-to-female ratio of 1:1.1). The overall prevalence of Schistosoma mansoni infection and non-typhoidal Salmonella carriage was 51.2% (95% CI: 48.2–54.1) and 3.4% (95% CI: 2.5–4.7) respectively, with 2.2% (95% CI: 1.5–3.2) of participants coinfected. The proportion of Salmonella carriage tended to be higher among Schistosoma mansoni infected participants compared to non-infected participants but this difference did not reach statistical significance (4.2% versus 2.6%, p = 0.132). However, the proportion of Salmonella carriage among participants with a heavy Schistosoma mansoni infection was significantly higher compared to those with a light and moderate infection (8.7% versus 3.2%, p = 0.012) and compared to Schistosoma mansoni negatives (8.7% versus 2.6%, p = 0.002). The 38 Salmonella isolates comprised five and four Enteritidis and Typhimurium serotypes respectively, the majority of them had MLVA types identical or similar to those observed among blood culture isolates. Conclusion Salmonella intestinal carriage was associated with a heavy intensity of Schistosoma mansoni infection. Further studies are needed to address causation., Author summary Salmonella intestinal carriage may be facilitated by infection with Schistosoma mansoni, which is a flatworm that lives in the small blood vessels of the human intestinal tract. This situation has been described in tropical low and middle income countries, where both pathogens are endemic. The present study aimed to assess the association of Salmonella intestinal carriage with Schistosoma mansoni infection in Kifua II village, located in the Kongo Central province of the Democratic republic of Congo. We collected stool samples from 1,108 inhabitants of the village and analysed them for Salmonella (culture) and Schistosoma mansoni eggs (microscopy). Our findings showed that 3.4% of the participants had Salmonella in their stool and 51.2% were infected with Schistosoma mansoni. There was no association between overall Schistosoma infection and Salmonella carriage but Salmonella carriage was significantly higher among participants with heavy Schistosoma infection compared to those with a light and moderate infection (8.7% versus 3.2%, p = 0.012) and compared to those without Schistosoma mansoni infection (8.7% versus 2.6%, p = 0.002). Further, we showed that some Salmonella isolates recovered from stool were very similar to isolates obtained from invasive infections (blood cultures from patients in a neighbouring hospital).
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- 2020
10. An African Salmonella Typhimurium ST313 sublineage with extensive drug-resistance and signatures of host adaptation
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Derek Pickard, Simon Clare, Katherine Harcourt, Eva Heinz, Jan Jacobs, Dauly Ngbonda, Elizabeth J. Klemm, Nicole E. Wheeler, Emily A. Lees, Marie-France Phoba, Stijn Deborggraeve, Tessa De Block, Sushmita Sridhar, Barbara Barbé, Koen Vandelannoote, Dadi Falay, Octavie Lunguya, Lisette Mbuyi Kalonji, Eve L. Coomber, Laura Maria Francisca Kuijpers, Gordon Dougan, Sandra Van Puyvelde, Van Puyvelde, Sandra [0000-0001-8434-5732], Heinz, Eva [0000-0003-4413-3756], Coomber, Eve L [0000-0002-7093-2263], Lees, Emily A [0000-0003-0229-7509], Wheeler, Nicole E [0000-0003-4599-9164], Klemm, Elizabeth J [0000-0002-2175-3752], Kuijpers, Laura [0000-0002-4480-7872], Jacobs, Jan [0000-0002-8657-6777], Deborggraeve, Stijn [0000-0003-1501-5355], Apollo - University of Cambridge Repository, Coomber, Eve L. [0000-0002-7093-2263], Lees, Emily A. [0000-0003-0229-7509], Wheeler, Nicole E. [0000-0003-4599-9164], and Klemm, Elizabeth J. [0000-0002-2175-3752]
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0301 basic medicine ,Salmonella typhimurium ,Salmonella ,THP-1 Cells ,631/326/325/2482 ,General Physics and Astronomy ,Drug resistance ,Azithromycin ,medicine.disease_cause ,706/134 ,wc_269 ,Antimicrobial resistance ,ANNOTATION ,38/70 ,DISEASE ,Mice ,Plasmid ,Ciprofloxacin ,Bacterial genetics ,Drug Resistance, Multiple, Bacterial ,NONTYPHOIDAL SALMONELLA ,EPIDEMIOLOGY ,129 ,lcsh:Science ,Phylogeny ,Developing world ,Multidisciplinary ,biology ,SPECTRUM BETA-LACTAMASE ,Adaptation, Physiological ,3. Good health ,Anti-Bacterial Agents ,Multidisciplinary Sciences ,Salmonella enterica ,INFECTIONS ,Democratic Republic of the Congo ,Science & Technology - Other Topics ,64/60 ,Host adaptation ,Engineering sciences. Technology ,medicine.drug ,Plasmids ,631/114/739 ,141 ,Science ,NUCLEOTIDE ,030106 microbiology ,13/106 ,45/23 ,wa_395 ,Microbial Sensitivity Tests ,SEQUENCE ,General Biochemistry, Genetics and Molecular Biology ,Article ,Microbiology ,qw_45 ,Cell Line ,03 medical and health sciences ,medicine ,Animals ,Humans ,qw_131 ,Whole genome sequencing ,Science & Technology ,45 ,Whole Genome Sequencing ,General Chemistry ,ENTERICA ,biology.organism_classification ,Multiple drug resistance ,Mice, Inbred C57BL ,030104 developmental biology ,692/699/255/1318 ,DISCOVERY ,Biofilms ,631/326/22/1434 ,lcsh:Q ,Bacterial infection - Abstract
Bloodstream infections by Salmonella enterica serovar Typhimurium constitute a major health burden in sub-Saharan Africa (SSA). These invasive non-typhoidal (iNTS) infections are dominated by isolates of the antibiotic resistance-associated sequence type (ST) 313. Here, we report emergence of ST313 sublineage II.1 in the Democratic Republic of the Congo. Sublineage II.1 exhibits extensive drug resistance, involving a combination of multidrug resistance, extended spectrum β-lactamase production and azithromycin resistance. ST313 lineage II.1 isolates harbour an IncHI2 plasmid we name pSTm-ST313-II.1, with one isolate also exhibiting decreased ciprofloxacin susceptibility. Whole genome sequencing reveals that ST313 II.1 isolates have accumulated genetic signatures potentially associated with altered pathogenicity and host adaptation, related to changes observed in biofilm formation and metabolic capacity. Sublineage II.1 emerged at the beginning of the 21st century and is involved in on-going outbreaks. Our data provide evidence of further evolution within the ST313 clade associated with iNTS in SSA., Invasive non-typhoidal Salmonella (iNTS) infections are dominated by antibiotic resistant isolates of the sequence type (ST) 313. Here, the authors identify the ST313 sublineage II.1 in the Democratic Republic of the Congo exhibiting extensive drug resistance and genetic signatures potentially associated with host adaptation.
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- 2019
11. Salmonella Typhi From Blood Cultures in the Democratic Republic of the Congo : A 10-Year Surveillance
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Stijn Deborggraeve, Barbara Barbé, Marianne A B van der Sande, Octavie Lunguya, Lisette Mbuyi Kalonji, Marie-France Phoba, Elise Monsieurs, Liselotte Hardy, Bieke Tack, Jan Jacobs, and Sandra Van Puyvelde
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Adult ,0301 basic medicine ,Microbiology (medical) ,Time Factors ,Adolescent ,Genotype ,030106 microbiology ,Bacteremia ,Supplement Articles ,Microbial Sensitivity Tests ,Salmonella typhi ,Salmonella Typhi ,Typhoid fever ,Young Adult ,03 medical and health sciences ,0302 clinical medicine ,Antibiotic resistance ,Drug Resistance, Multiple, Bacterial ,medicine ,Journal Article ,Humans ,Blood culture ,030212 general & internal medicine ,Child ,medicine.diagnostic_test ,business.industry ,Infant, Newborn ,Infant ,Middle Aged ,medicine.disease ,Virology ,Anti-Bacterial Agents ,3. Good health ,Infectious Diseases ,Reduced susceptibility ,Blood Culture ,Child, Preschool ,Epidemiological Monitoring ,Democratic Republic of the Congo ,surveillance ,Seasons ,business ,typhoid fever - Abstract
Background This study gives an overview of a decade (2007–2017) of hospital-based Salmonella Typhi bloodstream infection (BSI) surveillance in the Democratic Republic of the Congo (DRC), at 4 main sampling sites. Methods Blood cultures were sampled in hospital-admitted patients with suspected BSI, according to standardized clinical indications. The results of the surveillance period 2015–2017 were compiled with those of previous surveillance periods (2007–2010 and 2011–2014). Whole genome sequencing of isolates with decreased ciprofloxacin susceptibility (DCS) was performed. Results Salmonella Typhi was isolated in 1.4% (531/37 388) and 10.3% (531/5177) of suspected and culture-confirmed BSI episodes, respectively. Salmonella Typhi ranked first among the BSI pathogens in adults (n = 220), but was mostly (n = 301 [56.7%]) isolated from children, of which 72.1% (217/301) and 31.6% (95/301) were Conclusions Salmonella Typhi was a frequent cause of BSI in adults and children in DRC, with high rates of antibiotic resistance. Sustainable surveillance and implementation of vaccination are compelling.
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- 2019
12. Invasive Salmonella Infections at Multiple Surveillance Sites in the Democratic Republic of the Congo, 2011-2014
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Laura Maria Francisca Kuijpers, Octavie Lunguya, Wesley Mattheus, Jan Verhaegen, Lisette Mbuyi Kalonji, Dadi Falay, Chris Van Geet, Jan Jacobs, Annelies Post, Jean-Jacques Muyembe, Marie-France Phoba, Barbara Barbé, Dauly Ngbonda, Pieter-Jan Ceyssens, and Sophie Bertrand
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Microbiology (medical) ,nontyphoidal Salmonella ,Adult ,Male ,Salmonella typhimurium ,Salmonella ,antibiotic resistance ,Adolescent ,Salmonella enteritidis ,Salmonella infection ,bloodstream infection ,Bacteremia ,Microbial Sensitivity Tests ,Azithromycin ,Salmonella typhi ,medicine.disease_cause ,Salmonella Typhi ,beta-Lactamases ,Microbiology ,Young Adult ,Antibiotic resistance ,Ciprofloxacin ,Drug Resistance, Multiple, Bacterial ,medicine ,Humans ,Blood culture ,Child ,Aged ,medicine.diagnostic_test ,business.industry ,Infant ,Middle Aged ,bacterial infections and mycoses ,medicine.disease ,Anti-Bacterial Agents ,Multiple drug resistance ,Infectious Diseases ,Child, Preschool ,Epidemiological Monitoring ,Salmonella Infections ,Democratic Republic of the Congo ,Female ,Seasons ,business ,medicine.drug - Abstract
BACKGROUND This study reports the microbiological landscape of Salmonella Typhi and invasive nontyphoidal Salmonella (iNTS) in the Democratic Republic of the Congo (DRC). METHODS Blood cultures obtained from hospital-admitted patients suspected of bloodstream infection (BSI) in 4 of 11 provinces in DRC (Kinshasa, Bas-Congo, Equateur, and Orientale) were processed. Sampling had started in 2007; the results for the period 2011-2014 are reported. RESULTS Salmonella Typhi and iNTS were cultured from 194 (1.4%) and 840 (5.9%), respectively, of 14,110 BSI episodes and ranked first among BSI pathogens in adults (65/300 [21.7%]) and children (783/1901 [41.2%]), respectively. A total of 948 of 1034 (91.7%) isolates were available for analysis (164 Salmonella Typhi and 784 iNTS). Salmonella Typhimurium and Salmonella Enteritidis represented 386 (49.2%) and 391 (49.9%), respectively, of iNTS isolates, fluctuating over time and geography and increasing during the rainy season. Adults accounted for
- Published
- 2015
13. Direct association between rainfall and non-typhoidal Salmonella bloodstream infections in hospital-admitted children in the Democratic Republic of Congo
- Author
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Bieke Tack, Daniel Vita, Marie-France Phoba, Lisette Mbuyi-Kalonji, Liselotte Hardy, Barbara Barbé, Jan Jacobs, Octavie Lunguya, and Liesbet Jacobs
- Subjects
Medicine ,Science - Abstract
Abstract Non-typhoidal Salmonella (NTS) ranks first among causes of bloodstream infection in children under five years old in the Democratic Republic of Congo and has a case fatality rate of 15%. Main host-associated risk factors are Plasmodium falciparum malaria, anemia and malnutrition. NTS transmission in sub-Saharan Africa is poorly understood. NTS bloodstream infections mostly occur during the rainy season, which may reflect seasonal variation in either environmental transmission or host susceptibility. We hypothesized that environment- and host-associated factors contribute independently to the seasonal variation in NTS bloodstream infections in children under five years old admitted to Kisantu referral hospital in 2013–2019. We used remotely sensed rainfall and temperature data as proxies for environmental factors and hospital data for host-associated factors. We used principal component analysis to disentangle the interrelated environment- and host-associated factors. With timeseries regression, we demonstrated a direct association between rainfall and NTS variation, independent of host-associated factors. While the latter explained 17.5% of NTS variation, rainfall explained an additional 9%. The direct association with rainfall points to environmental NTS transmission, which should be explored by environmental sampling studies. Environmental and climate change may increase NTS transmission directly or via host susceptibility, which highlights the importance of preventive public health interventions.
- Published
- 2021
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