1. [Creation of transgenic sugar beet lines expressing insect pest resistance genes cry1C and cry2A].
- Author
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Litvin DI, Sivura VV, Kurilo VV, Oleneva VD, Emets AI, and Blium IaB
- Subjects
- Animals, Bacillus thuringiensis chemistry, Bacillus thuringiensis genetics, Bacillus thuringiensis Toxins, Bacterial Proteins metabolism, Benzyl Compounds pharmacology, Beta vulgaris drug effects, Beta vulgaris immunology, Beta vulgaris parasitology, Diptera physiology, Endotoxins metabolism, Gene Expression, Gene Transfer Techniques, Genetic Vectors, Genome, Plant, Hemolysin Proteins metabolism, Indoles pharmacology, Plant Diseases immunology, Plant Diseases parasitology, Plant Leaves drug effects, Plant Leaves immunology, Plant Leaves parasitology, Plants, Genetically Modified, Purines pharmacology, Transgenes, Bacterial Proteins genetics, Beta vulgaris genetics, Diptera pathogenicity, Endotoxins genetics, Hemolysin Proteins genetics, Pest Control, Biological, Plant Diseases genetics, Plant Leaves genetics
- Abstract
Impact of insect pests makes a significant limitation of the sugar beet crop yield. Integration of cry-genes of Bacillus thuringiensis into plant genome is one of the promising strategies to ensure plant resistance. The aim of this work was to obtain sugar beet lines (based on the MM 1/2 line) transformed with cry2A and cry1Cgenes. We have optimized transformation protocol and direct plant let regeneration protocol from leaf explants using 1 mg/l benzylaminopurine as well as 0,25 mg/l benzylaminopurine and 0,1 mg/l indole-butyric acid. Consequently, transgenic sugar beet lines transformed with vector constructs pRD400-cry1C and pRD400-cry2A have been obtained. PCR analysis revealed integration of cry2A and cry1C into genome of transgenic lines and expression of these genes in leaf tissues was shown by reverse transcription PCR.
- Published
- 2014