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In recent studies, stem cell-based therapy is a potential new approach in the treatment of stroke. The mechanism of human umbilical cord mesenchymal stem cell (hUMSC) transplantation as one of the new approaches in the treatment of ischemic stroke is still unclear. The aim of this study was to determine the traits of immune responses during stroke progression after treatment with human umbilical cord blood MSCs by bioinformatics, to predict potential prognostic biomarkers that could lead to sex differences, and to reveal potential therapeutic targets. The microarray dataset GSE78731 (mRNA profile) of middle cerebral artery occlusion (MCAO) rats was obtained from the Gene Expression Omnibus (GEO) database. First, two potentially expressed genes (DEGs) were screened using the Bioconductor R package. Ultimately, 30 specific DEGs were obtained (22 upregulated and 353 downregulated). Next, bioinformatic analysis was performed on these specific DEGs. We performed a comparison for the differentially expressed genes screened from between the hUMSC and MCAO groups. Gene Ontology enrichment and pathway enrichment analyses were then performed for annotation and visualization. Gene Ontology (GO) functional annotation analysis shows that DEGs are mainly enriched in leukocyte migration, neutrophil activation, neutrophil degranulation, the external side of plasma membrane, cytokine receptor binding, and carbohydrate binding. KEGG pathway enrichment analysis showed that the first 5 enrichment pathways were cytokine-cytokine receptor interaction, chemokine signal pathway, viral protein interaction with cytokine and cytokine receptor, cell adhesion molecules (CAMs), and phagosome. The top 10 key genes of the constructed PPI network were screened, including Cybb, Ccl2, Cd68, Ptprc, C5ar1, Il-1b, Tlr2, Itgb2, Itgax, and Cd44. In summary, hUMSC is likely to be a promising means of treating IS by immunomodulation. [ABSTRACT FROM AUTHOR]

Background: A decrease in hippocampal neurogenesis is considered an important cause of cognitive impairment, while changes in mossy fiber sprouting are closely related to development of spontaneous recurrent seizures in chronic temporal lobe epilepsy (TLE). Racemic l-3-n-butylphthalide (DL-NBP) can alleviate cognitive impairment in ischemic stroke and Alzheimer's disease by promoting neurogenesis. DL-NBP treatment can also improve cognitive function and reduce seizure incidence in chronic epileptic mice. However, the mechanisms of action of DL-NBP remain unclear. The aim of the present study was to examine the effects of DL-NBP on mossy fiber sprouting, hippocampal neurogenesis, spontaneous epileptic seizures, and cognitive functioning in the chronic phase of TLE.Methods: Nissl staining was used to evaluate hippocampal injury, while immunofluorescent staining was used to analyze hippocampal neurogenesis. The duration of spontaneous seizures was measured by electroencephalography. The Morris water maze was used to evaluate cognitive function. Timm staining was used to assess mossy fiber sprouting.Results: TLE animals showed reduced proliferation of newborn neurons, cognitive dysfunction, and spontaneous seizures. Treatment with DL-NBP after TLE increased the proliferation and survival of newborn neurons in the dentate gyrus, reversed the neural loss in the hippocampus, alleviated cognitive impairments, and decreased mossy fiber sprouting and long-term spontaneous seizure activity.Conclusions: We provided pathophysiological and morphological evidence that DL-NBP might be a useful therapeutic for the treatment of TLE. [ABSTRACT FROM AUTHOR]

Stroke often leads to neuronal injury and neurological functional deficits. Whilst spontaneous neurogenesis and axon regeneration are induced by ischemic stroke, effective pharmacological treatments are also essential for the improvement of neuroplasticity and functional recovery after stroke. However, no pharmacological therapy has been demonstrated to be able to effectively improve the functional recovery after stroke. Bumetanide is a specific Na+-K+-Cl– co-transporter inhibitor which can maintain chloride homeostasis in neurons. Therefore, many studies have focused on this drug's effect in stroke recovery in recent years. Here, we first review the function of Na+-K+-Cl– co-transporter in neurons, then how bumetanide's role in reducing brain damage, promoting neuroplasticity, leading to functional recovery after stroke, is elucidated. Finally, we discuss current limitations of bumetanide's efficiency and their potential solutions. These results may provide new avenues for further exploring mechanisms of post-stroke functional recovery as well as promising therapeutic targets for functional disability rehabilitation after ischemic stroke. [ABSTRACT FROM AUTHOR]

Cadmium (Cd) pollution poses a growing threat to rice production in acidic paddies. In south China, a common agricultural practice involves the combined utilization of Chinese milk vetch (M) and rice straw (R). However, it is unclear how the addition of lime to these amendments affects Cd bioavailability and accumulation in soil. Control (CK), chemical fertilizer (F), Chinese milk vetch + rice straw + chemical fertilizer (MRF), and Chinese milk vetch + rice straw + chemical fertilizer + lime (MRFL) treatments were applied to develop a kind of green, efficient, and practical amendment for acidic paddies. We conducted a microplot experiment to explore Cd immobilization in paddy soil and the Cd content in rice grains with these treatments. The results showed that compared with F, the rice Cd in the MRF and MRFL treatments were significantly decreased by 51.7% and 65.2% in early rice and 23.0% and 43.3% in late rice, respectively. Both the MRF and MRFL treatments significantly reduced soil available Cd and weak acid-extractable cadmium (Aci-Cd) concentrations and increased soil organic matter (SOM), exchangeable cation concentrations, and pH, which converted Cd into a stable form in soil. In addition, the MRF and MRFL treatments increased soil pH value by reducing soil exchangeable hydrogen ion concentration (E-H). Additionally, recombination of Cd forms was the primary factor in the reduction in available Cd concentration according to partial least squares path modeling (PLS-PM) analysis. The Cd concentration of rice grains was primarily associated with soil available Cd, soil pH value, and SOM. Overall, these results provide useful data and novel insights into reducing rice grain Cd in south China. [ABSTRACT FROM AUTHOR]

Chemotherapy‐induced peripheral neuropathy (CIPN) is a common side effect during the course of cancer treatment, which is mainly manifested as a series of sensory abnormalities. At present, there are no recommended prevention or treatment strategies, and the underlying mechanisms are unclear. The ketogenic diet (KD), a special diet that is high in fat and low in carbohydrate intake, shows good therapeutic potential in children with epilepsy. In this study, it was found that KD significantly prevented paclitaxel‐induced neuropathic nociception. Using the GSE113941 database, 281 differentially expressed genes (DEGs) were found in an animal model of CIPN and controls. The DEGs were mainly enriched in peroxisome proliferator activated receptor (PPAR) and oxidative phosphorylation signalling pathways. As a main regulatory pathway of lipid metabolism, the PPARγ signalling pathway was significantly upregulated in the KD model. In addition, KD also inhibited the expression of pro‐inflammatory cytokines and the TLR4/NF‐κB signalling pathway in the dorsal root ganglion (DRG) in paclitaxel‐treated rats. In vitro, rat primary DRG neurons were used to investigate the role of PPARγ in paclitaxel‐induced neurotoxicity. It was found that PPARγ agonist rosiglitazone significantly protected DRG neurons against cell apoptosis and reactive oxygen species generation induced by paclitaxel administration. Therefore, KD is a prospective treatment option when applied as a dietary intervention in the prevention and treatment of paclitaxel‐induced neuropathic nociception, possibly through the activation of PPARγ and its neuroprotective functions. [ABSTRACT FROM AUTHOR]