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Your search keyword '"Liu, Guomu"' showing total 17 results
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17 results on '"Liu, Guomu"'

13. Mucin1 promotes the migration and invasion of hepatocellular carcinoma cells via JNK-mediated phosphorylation of Smad2 at the C-terminal and linker regions

Author

Wang, Juan, primary, Liu, Guomu, additional, Li, Qiongshu, additional, Wang, Fang, additional, Xie, Fei, additional, Zhai, Ruiping, additional, Guo, Yingying, additional, Chen, Tanxiu, additional, Zhang, Nannan, additional, Ni, Weihua, additional, Yuan, Hongyan, additional, and Tai, Guixiang, additional

Published
2015
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17. Cytotoxicity of various types of gold-mesoporous silica nanoparticles in human breast cancer cells.

Author

Liu G, Li Q, Ni W, Zhang N, Zheng X, Wang Y, Shao D, and Tai G

Subjects
Animals, Brain cytology, Brain drug effects, Brain metabolism, Breast Neoplasms metabolism, Breast Neoplasms pathology, Caspase 3 metabolism, Caspase 9 metabolism, Cells, Cultured, Cytochromes c metabolism, Endothelium, Vascular cytology, Endothelium, Vascular drug effects, Endothelium, Vascular metabolism, Female, Gene Expression Profiling, Humans, MAP Kinase Signaling System drug effects, MCF-7 Cells, Membrane Potential, Mitochondrial drug effects, Mitochondria drug effects, Mitochondria metabolism, Nanoparticles chemistry, Phosphorylation drug effects, Rats, Reactive Oxygen Species metabolism, Real-Time Polymerase Chain Reaction, Apoptosis drug effects, Breast Neoplasms drug therapy, Gold chemistry, Nanoparticles administration & dosage, Silicon Dioxide chemistry
Abstract

Recently, gold nanoparticles (AuNPs) have shown promising biological applications due to their unique electronic and optical properties. However, the potential toxicity of AuNPs remains a major hurdle that impedes their use in clinical settings. Mesoporous silica is very suitable for the use as a coating material for AuNPs and might not only reduce the cytotoxicity of cetyltrimethylammonium bromide-coated AuNPs but might also facilitate the loading and delivery of drugs. Herein, three types of rod-like gold-mesoporous silica nanoparticles (termed bare AuNPs, core-shell Au@mSiO2NPs, and Janus Au@mSiO2NPs) were specially designed, and the effects of these AuNPs on cellular uptake, toxic behavior, and mechanism were then systematically studied. Our results indicate that bare AuNPs exerted higher toxicity than the Au@mSiO2NPs and that Janus Au@mSiO2NPs exhibited the lowest toxicity in human breast cancer MCF-7 cells, consistent with the endocytosis capacity of the nanoparticles, which followed the order, bare AuNPs > core-shell Au@mSiO2NPs > Janus Au@mSiO2NPs. More importantly, the AuNPs-induced apoptosis of MCF-7 cells exhibited features that were characteristic of intracellular reactive oxygen species (ROS) generation, activation of c-Jun-N-terminal kinase (JNK) phosphorylation, an enhanced Bax-to-Bcl-2 ratio, and loss of the mitochondrial membrane potential. Simultaneously, cytochrome c was released from mitochondria, and the caspase-3/9 cascade was activated. Moreover, both ROS scavenger (N-acetylcysteine) and JNK inhibitor (SP600125) partly blocked the induction of apoptosis in all AuNPs-treated cells. Taken together, these findings suggest that all AuNPs induce apoptosis through the ROS-/JNK-mediated mitochondrial pathway. Thus, Janus Au@mSiO2NPs exhibit the potential for applications in biomedicine, thus aiding the clinical translation of AuNPs.

Published
2015
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