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Pulsatile shear (PS) and oscillatory shear (OS) elicit distinct mechanotransduction signals that maintain endothelial homeostasis or induce endothelial dysfunction, respectively. A subset of microRNAs (miRs) in vascular endothelial cells (ECs) are differentially regulated by PS and OS, but the regulation of the miR processing and its implications in EC biology by shear stress are poorly understood. From a systematic in silico analysis for RNA binding proteins that regulate miR processing, we found that nucleolin (NCL) is a major regulator of miR processing in response to OS and essential for the maturation of miR-93 and miR-484 that target mRNAs encoding Krüppel-like factor 2 (KLF2) and endothelial nitric oxide synthase (eNOS). Additionally, anti-miR-93 and anti-miR-484 restore KLF2 and eNOS expression and NO bioavailability in ECs under OS. Analysis of posttranslational modifications of NCL identified that serine 328 (S328) phosphorylation by AMP-activated protein kinase (AMPK) was a major PS-activated event. AMPK phosphorylation of NCL sequesters it in the nucleus, thereby inhibiting miR-93 and miR-484 processing and their subsequent targeting of KLF2 and eNOS mRNA. Elevated levels of miR-93 and miR-484 were found in sera collected from individuals afflicted with coronary artery disease in two cohorts. These findings provide translational relevance of the AMPK-NCL-miR-93/miR-484 axis in miRNA processing in EC health and coronary artery disease.

In this paper, we analyze the light variation of KIC 8840638 using high-precision time-series data from the Kepler mission. The analysis reveals that this target is a new detached eclipsing binary system with a δ Scuti component, rather than a single δ Scuti star as previously known. The frequency analysis of short-cadence data reveals 95 significant frequencies, most of which lie in a frequency range of 23−32 day−1. Among them, seven independent frequencies are detected in the typical frequency range of δ Scuti stars, and they are identified as pressure modes. In addition, a possible large separation value of Δ ν = 36.5 ± 0.1 μ Hz is also detected with the Fourier transform (FT) and autocorrelation function (AC) analysis. The orbital frequency f orb (= 0.320008 day−1) and its harmonics are also detected directly in the frequency spectrum. The binary modelings derived from PHOEBE indicate that this binary system is in detached configurations with a mass ratio of q = 0.33 − 0.04 + 0.06 , an inclination angle of 40.19 − 2.84 + 3.96 ​​​​​°. The derived parameters and binary evolutionary model suggest that the primary star is an object on the verge of leaving the main sequence with a temperature of ∼7600 K, while the secondary appears to be a cool component entering the giant branch with a temperature ∼3100 K lower than the primary. Moreover, this system may have undergone a mass ratio reversal, where the more massive star is the gainer component and the less massive one is the donor star. [ABSTRACT FROM AUTHOR]

Understanding the mechanical response characteristics and determining the optimized process conditions is critical to mitigate crystal impacts during resonant acoustic mixing (RAM). Therefore, high‐melting explosive (HMX) crystal collisions with container walls under different RAM accelerations were investigated through simulations and experiments. The HMX crystal damages after RAM were assessed using microscopic imaging, small‐angle X‐ray scattering, and Brunauer–Emmett–Teller tests. Results show that crystal fractures observed in steel containers can be prevented by using low‐modulus polytetrafluoroethylene containers. Rough containers reduce internal damage but increase surface abrasion. Lower RAM accelerations, shorter RAM durations, and low‐modulus containers can mitigate HMX crystal damage. [ABSTRACT FROM AUTHOR]

There are few comparisons of Modic changes (MCs) in the lumbar and cervical spine.Compare the prevalence of MCs in the lumbar and cervical spine, and determine how MC prevalence depends on spinal pain, age, disc degeneration, spinal level, and the presence or absence of kyphosis.Retrospective clinical survey.Magnetic resonance images (MRIs) were compared from five patient groups: 1. 1223 patients with low-back pain/radiculopathy only; 2. 1023 patients with neck pain/radiculopathy only; 3. 497 patients with concurrent low-back and neck symptoms; 4. 304 asymptomatic subjects with lumbar MRIs; and 5. 120 asymptomatic subjects with cervical MRIs.The prevalence of MCs was higher in those with spinal pain than in those without, both in the lumbar spine (21.0% vs 10.5%) and cervical spine (8.8% vs 3.3%). Type II MCs were most common and Type III were least common in all groups. The prevalence of lumbar MCs in people with back pain was little affected by the presence of concurrent neck pain, and the same was true for the prevalence of cervical MCs in people with neck pain with or without concurrent back pain. When symptomatic patients were reclassified into two groups (back pain, neck pain), the prevalence of lumbar MCs in people with back pain was greater than that of cervical MCs in people with neck pain. The prevalence of lumbar and cervical MCs increased with age, disc degeneration, (descending) spinal level, and increased kyphosis.There is a significantly higher prevalence of MCs in patients with back and neck pain. The reported association with increased kyphosis (flat back) is novel.

Objective: Surgery is indicated when antibiotic treatment fails in pyogenic spondylodiscitis, which is caused by pathogens such as the Staphylococcus species. The aim of the present study was to investigate the efficacy and safety of the oblique lateral interbody fusion (OLIF) corridor approach combined with posterior pedicle screw fixation for treating pyogenic spondylodiscitis. Methods: This was a retrospective case series study. A total of 11 patients with an average age of 60.7 years (range, 40–70 years; 10 males and 1 females) with lumbar pyogenic spondylodiscitis who underwent single‐stage debridement and reconstruction using the OLIF corridor combined with posterior pedicle screw fixation were recruited in our study from June 2016 to July 2017. All patients had single‐level pyogenic spondylodiscitis between T12 and L5. The baseline data, perioperative outcomes (operative time, intra‐operative blood loss, and intra‐operative complication), postoperative laboratory tests (erythrocyte sedimentation rate [ESR], C‐reactive protein [CRP], white blood count [WBC], and tissue culture results), long‐term complications (recurrence, fixation failure, and bony non‐fusion rates), and duration of antibiotic administration were reviewed. Outcomes evaluated using a variety of scales including visual analog scale (VAS) score and Oswestry disability index (ODI), were compared pre‐operatively and post‐operatively. Results: The mean follow‐up period of time was 18.3 months. The average operative time and intra‐operative blood loss were 217.0 ± 91.91 min and 220.9 ± 166.10 mL, respectively. There were no intra‐operative complications, except in 1 patient who encountered somatosensory evoked potentials changes and 1 patient who had motor evoked potentials changes, both without post‐surgery neurological deficits. Causative organisms were identified in 4 patients: Staphylococcus aureus in 1 patient and Streptococcus in 3 patients. At approximately 8.8 weeks after surgery, WBC, CRP, and ESR had returned to normal levels. All patients were pain free with no recurring infection. There was no fixation failure during follow up. Solid bony fusions were observed in all cases within 6 months. At the final follow up, the mean VAS (0.6 ± 0.69) and ODI (14.4 ± 4.27) were significantly lower than those before surgery (P < 0.05). Conclusion: One‐stage debridement with autogenous iliac bone graft through the OLIF corridor combined with posterior pedicle screw fixation is effective and safe for single‐level spontaneous lumbar pyogenic spondylodiscitis after antibiotic treatment fails. [ABSTRACT FROM AUTHOR]