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Supplemental material, sj-pdf-1-chl-10.1177_17475198221115020 for The practical acetylation of nucleosides using acetic anhydride/acetic acid as a reusable solvent by Ran Xia, Chao Xia, Ying-Xing Yang, Li-Jie Liu, Lei-Shan Chen and Li-Ping Sun in Journal of Chemical Research

Deng, Lun-Sha, Yu, Wen-Jie, Zeng, Nian-Kai, Liu, Li-Jie, Liu, Li-Yue, Fan, Yu-Guang (2021): Inosperma subsphaerosporum (Inocybaceae), a new species from Hainan, tropical China. Phytotaxa 502 (2): 169-178, DOI: 10.11646/phytotaxa.502.2.5, URL: http://dx.doi.org/10.11646/phytotaxa.502.2.5

Inosperma subsphaerosporum Y.G. Fan, L.S. Deng, W.J. Yu & L.Y. Liu sp. nov. Figs.2–4 MycoBank: MB838448 Etymology: The specific epithet “ subsphaerosporum ” refers to the shape of basidiospores. Diagnosis: Differs from I. carnosibulbosum by having more globose basidiospores and clavate to cylindricalclavate cheilocystidia. Type:— CHINA. Hainan Province: Ledong Li Autonomous County, Yinggeling substation of Hainan Tropical Rainforest National Park, 18°53ʹ27.21′′N, 109°19ʹ36.87′′E, elev. 400 m, 13 July 2020, L.-Y. Liu & Y.-G. Fan, FYG5848, FHMU3153 (holotype), GenBank nos.: MT 072898 (ITS), MT 071203 (LSU), MT 076849 (rpb 2). Description:— Basidiomata medium-sized. Pileus conical when young, then convex to umbonate-convex, finally plano-convex to applanate with a subacute or occasionally obtuse umbo; margin initially incurved and then decurved for a long time, never straight; surface dry, initially smooth with a thin whitish veil layer around the disc, becoming appressed-scaly with radial patches of veil remnants, fibrillose-rimulose elsewhere, margin rimose to strongly split in age; yellowish brown (6C6) to chocolate brown (6E6) around the center, yellowish white (5A4) to beige (5A2) towards margin; uniformly brownish yellow (4A4) to goldish yellow (4A7) when overmatured. Lamellae crowded, adnexed, alternating with several tiers of lamellula, 2–4 (6) mm wide, dirty-white (5A1) or beige (5A2) at first, then grayish white (6B1) to brownish gray (6C2), brownish yellow (6B4) to brown (6D6) in age; edge pallid (5A1), fimbricate to finely serrate. Stipe 55–115 × 6–10 (12) mm, terete, equal at upper half with a slightly swollen apex, thicker downwards with a swollen base; recurved squamulose at the apex, longitudinally fibrillose with sparse protruding fibrils downwards, base pure white (6A1) with tomentose hyphae; ivory white (7A1) to grayish white (6B1), background brownish or pinkish beneath the cuticle. Context solid, fleshy in pileus, ivory white, with brownish (5C6) tinge under the umbo, becoming brownish (5D8) with age, 4–8 mm thick at the center, 2–3 mm thick at mid-radius; fibrillose and striate in the stipe, lightly brownish or pinkish (6B8). Odor slightly salty or mild. Basidiospores [115/5/3] (5–) 6–7.5(–8) × 5–6(–6.5) μm, Q = 1.00–1.27 (–1.30), Q m = 1.13, subglobose to globose, occasionally broadly ellipsoid, smooth, yellowish or tan, indistinct small apiculus, inner with a large circular yellow oil-droplet. Basidia 26–31 × 7–8 μm, clavate to broadly clavate, occasionally obtuse at the apex and slightly tapering at the base, 4-spored, sterigmata 3–4.5 μm in length, hyaline to yellowish, with one or more bright yellow, oily inclusions when mature. Pleurocystidia absent. Cheilocystidia 27–50 × 8–11 μm, abundant, versiform, clavate, cylindricalclavate or occasionally broadly clavate to fusiform, often with obtuse apex, or narrowly tapered into capitate or subcapitate at the apex, septate and often constricted at septa, mostly thin-walled, some with walls up to 1 μm thick at the apex or towards the middle, colorless, at times with yellow intracellular contents. Hymenophoral trama 43–77 μm thick, regular to sub-regular, colorless to slightly yellowish, composed of cylindric to inflated hyphae 19–32 μm wide. Pileipellis a cutis, goldish yellow or tan, composed of sub-regular, thin-walled, yellowish, cylindrical hyphae, 7–10 μm wide, slightly encrusted. Stipitipellis regularly arranged, yellowish, disrupted with entangled, extended hyphae, 4–10 μm wide, terminal elements usually inflated to cheilocystidoid cells 39–62 × 10–21 μm, clavate to elongate or broadly clavate, hyaline, obtuse at the apex, occasionally capitate at the apex, thin-walled. Oleiferous hyphae 5–10 μm wide, present in pileus and stipe trama, yellow or bright yellow, smooth, bent, diverticulate, occasionally branched. Clamp connections present, common in all tissues. Habitat: gregarious in small groups or scattered along roadsides under fagaceous trees. Distribution: Known only from the type locality in Hainan Province, China. Additional specimen examined: CHINA. Hainan Province, Ledong Li Autonomous County, Yinggeling substation of Hainan Tropical Rainforest National Forest Park, 18°53ʹ27.21′′N, 109°19ʹ36.87′′E, elev. 400 m, 13 Jul. 2020, under fagaceous trees, L.-Y. Liu & N.-K. Zeng, FYG5846 (FMHU 3155), Y.-G. Fan & L.-S. Deng, FYG5847 (FMHU 3154).

We investigated the ability of microRNA-93 (miR-93) to influence proliferation, invasion, migration, and apoptosisofrenal cell carcinoma (RCC) cells via transforming growth factor-β/solvated metal atom dispersed (TGF-β/Smad) signaling by targeting runt-related transcription factor 3 (RUNX3). RCC tissues with corresponding adjacent normal tissues were collected from 249 RCC patients. And normal renal tissues were collected from patients without RCC who received nephrectomy. The RCC cell line ACHN was treated with miR-93 mimic, mimic-negative control (NC), miR-93 inhibitor, inhibitor-NC, and miR-93 inhibitor + small interfering RNA (siRNA) against RUNX3 (si-RUNX3). Expression of miR-93, RUNX3, TGF-β, and Smad4 were evaluated by quantitative real-time polymerase chain reaction (qRT-PCR) and Western blotting. Cell proliferation was assessed by the Metallothioneins (MTS) assay, cell invasion by the wound-healing assay, cell migration by the Transwell assay, and cell cycle and apoptosis by flow cytometry. Compared with normal renal tissues, the expression of miR-93 and TGF-β were higher while that of RUNX3 and Smad4 were low in RCC and adjacent normal tissues (all P

The overwintering survival ratio of the cultivar Dongnongdongmai 1 with strong cold-resistance in paramos of Heilongjiang Province in China are over 85%. The tillering nodes are the most important organs for overwintering survival of winter wheat, because there are more substances associated with cold resistance in tillering nodes than those in leaves and roots. Proteins in the tillering nodes of the cold-resistant cultivar Dongnongdongmai 1 grown under field conditions with or without any low-temperature stress were analyzed by 2–dimensional electrophoresis and identified by mass spectrometry. In the range of pH 4–7, the expression of 37 proteins showed obvious difference (±more than two fold) in the proteomic maps of cold-stressed and non-stressed tillering nodes, including a new protein spot. All proteins exhibiting the difference in expression were identified using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, followed by a database search for protein identification and function prediction. Five groups of proteins were confirmed, namely stress-related proteins (22%), metabolism-associated proteins (35%), and signaling molecules (24%), cell wall-binding proteins (5%), unclear proteins (14%). This indicated that tillering node cells supported the energy requirements of plant growth and stress resistance by signal transduction adapting to metabolism and structure.