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115 results on '"Liu, Shao-Ping"'

15. Effect of Evodiamine on Inducing Apoptosis of Human Gastric Cancer Cell Line SGC-7901 in Vitro

Author

LIU Shao-ping, QIAN Wen-jun, and MAO Wei-dong

Subjects
Evodiamine, lcsh:R, lcsh:Medicine, Apoptosis, Cell cycle, Gastric cancer
Abstract

Objective: To explore the proliferation inhibition and apoptosis-inducing effect of evodiamine in human gastric cancer SGC-7901 cells. Methods: After 48 or 24 h exposure to different concentrations of evodiamine, cell proliferation was analyzed using tetrazolium blue (MTT) assay while apoptosis and cell-cycle phase distribution using flow cytometry. Results: In 0.01～30.00 μg/mL range of concentrations, evodiamine inhibited the proliferation of SGC-7901 cells in dose-dependent manner, and the overall mean IC50 was (3.79±0.16) μg/mL; the apoptosis rate was increased from 3.4% to 7.0%, 13.8% and 36.3% at concentrations of 0, 0.5, 1.5 and 30 μg/mL of evodiamine, respectively; the percentage of cells accumulated in G2/M phase was increased from 17.26% to 98.92% in the cells treated with evodiamine for 24 h in 0.01～30.00 μg/mL range of concentrations. Conclusion: Evodiamine can inhibit the proliferation, induce apoptosis in human gastric cancer cell line SGC-7901 in vitro and arrest the cell cycle at the G2/M phase.

Published
2014

22. Quantum dot-based quantitative immunofluorescence detection and spectrum analysis of epidermal growth factor receptor in breast cancer tissue arrays

Author

Yang,Xue-Qin, Chen,Chuang, Peng,Chun-Wei, Hou,Jin-Xuan, Liu,Shao-Ping, Qi,Chu-Bo, Gong,Yi-Ping, Zhu,Xiao-Bo, Pang,Dai-Wen, Li,Yan, Yang,Xue-Qin, Chen,Chuang, Peng,Chun-Wei, Hou,Jin-Xuan, Liu,Shao-Ping, Qi,Chu-Bo, Gong,Yi-Ping, Zhu,Xiao-Bo, Pang,Dai-Wen, and Li,Yan

Abstract

Xue-Qin Yang1,2, Chuang Chen1, Chun-Wei Peng1, Jin-Xuan Hou1, Shao-Ping Liu1, Chu-Bo Qi3, Yi-Ping Gong4, Xiao-Bo Zhu5, Dai-Wen Pang6, Yan Li1 1Department of Oncology, Zhongnan Hospital of Wuhan University, Hubei Key Laboratory on Tumor Biological Behaviors and Hubei Cancer Clinical Study Center, Wuhan; 2Medical School of Jingchu University of Technology, Jingmen; 3Department of Pathology; 4Department of Breast Surgery, Hubei Cancer Hospital, Wuhan; 5Wuhan Jiayuan Quantum Dots Co Ltd and Wuhan Tumor Nanometer Diagnosis Engineering Research Center, Wuhan; 6Key Laboratory of Analytical Chemistry for Biology and Medicine, College of Chemistry and Molecular Sciences, and State Key Laboratory of Virology, Wuhan University, Wuhan, People's Republic of China Background: The epidermal growth factor receptor (EGFR) is a promising therapeutic target in cancer, but its clinical value in breast cancer remains controversial. Our previous studies have found that quantitative analysis of biomarkers with quantum dot-based nanotechnology had better detection performance than conventional immunohistochemistry. The present study was undertaken to investigate the prognostic value of EGFR in breast cancer using quantum dot-based quantitative spectral analysis. Methods: EGFR expression in 65 breast cancer specimens was detected by immunohistochemistry and quantum dot-immunohistochemistry, and comparisons were made between the two methods. EGFR expression in tissue microarrays of 240 breast cancer patients was then detected by quantum dot-immunohistochemistry and spectral analysis. The prognostic value of EGFR immunofluorescence area (EGFR area) for five-year recurrence-free survival was investigated. Results: The same antigen localization, high correlation of staining rates (r = 0.914), and high agreement of measurement (κ= 0.848) of EGFR expression in breast cancer were found by quantum dot-immunohistochemistry and immunohistochemistry. The EGFR area showed significant

Published
2011

49. Effects of surgically implanted dummy ultrasonic transmitters on grass carp Ctenopharyngodon idella.

Author

LUO Hong-wei, DUAN Xin-bin, WANG Sheng, TIAN Hui-wu, LIU Shao-ping, and CHEN Da-qing

Abstract

In order to study the effects of surgically implanted dummy ultrasonic transmitters on grass carp Ctenopharyngodon idella, two experiments were conducted from October 2010 to December 2010 and November 2010 to October 2011. The results showed that surgical implantation of dummy ultrasonic transmitters had a significant negative influence on the growth of grass carp within 30 days following the surgery. However, the negative influence after 30 days faded away. One and two fish died during the 60-day and 360-day experiments, equivalent to the mortalities of 4. 2% and 6. 7%, respectively. All fish incisions were completely healed in 30 days after surgery, and all sutures were lost in 360 days after surgery. Two and one fish expelled the transmitters through the unclosed wound during the 60 days and 360 days, and the discharges were 8. 7% and 3. 3%, respectively. All the remaining transmitters were encapsulated in fibrous capsules and adhered to body wall, intestinal tract or viscera multiply. The result suggested that surgical implantation of ultrasonic transmitters could be applied to the ultrasonic telemetry research on grass carp. Nevertheless, the grass carp should be given for at least 30 days with the purpose of incision healing and growth recovery from the surgical procedure. [ABSTRACT FROM AUTHOR]

Published
2014

50. Effects of transmitter's surgical implantation on fish: Research progress.

Author

LUO Hong-wei, DUAN Xin-bin, LIU Shao-ping, and CHEN Da-qing

Abstract

Surgical implantation is generally accepted as the preferred attachment method for long-term fish telemetry study, but the negative effects of this surgical implantation on fish should not be ignored. The key for the successful fish telemetry study is that the existence and attachment of the transmitters in the experimental fish would not have negative effects on the normal fish physiology or behavior. Therefore, it is necessary to assess the potential negative effects of surgical implantation of transmitter on experimental fish prior to telemetry monitoring. This paper reviewed the overseas research progress in the effects of surgical implantation of transmitter on fish, and discussed the possible negative effects and affecting degrees of transmitters, surgical procedure, environmental factors, and anthropogenic factors in the process of transmitter's surgical implantation in fish. Some suggestions were put forward to reduce the potential negative effects, and the research directions of surgically implanted transmitters in fish in China were prospected. [ABSTRACT FROM AUTHOR]

Published
2013

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