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2. Uncertainty evaluation for the determination of three pyrethroid pesticide residues in fresh tea leaves

Author

ZHANG Li, LIU Teng-fei, YAO Ling-yi, CHEN Yu-yang, and JIANG Li

Subjects
gas chromatography, fresh tea leaves, pyrethroid pesticides, uncertainty, Food processing and manufacture, TP368-456
Abstract

Objective: The uncertainty of determination of three pyrethroid pesticides （cypermethrin, fenvalerate and deltamethrin） in fresh tea leaves by gas chromatography was evaluated. Methods: A mathematical model was established according to the determination process of the experiment, and the uncertainties caused by various factors in the model were identified. The sources are mainly the following 7 aspects: repeated determination of samples, fitness of standard curve, recovery rate, standard solution preparation, sample weighing and pretreatment, and the measurement of instrument. Results: At the spiking level of 0.10 mg/kg, the expanded uncertainty of measured values of cypermethrin, fenvalerate, and deltamethrin were 0.011, 0.011 and 0.012 mg/kg, respectively. The detection results of three pesticides in fresh tea leaves in 95% confidence interval could be expressed as cypermethrin: （0.093±0.011） mg/kg, fenvalerate: （0.094±0.011） mg/kg, deltamethrin: （0.098±0.012） mg/kg （k=2）. Conclusion: The uncertainties of the method were mainly introduced by standard solution preparation, calibration curve fitting and recovery rate, indicating that these aspects should be controlled strictly in the following sample analysis.

Published
2023
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7. A regulatory mutant on TRIM26 conferring the risk of nasopharyngeal carcinoma by inducing low immune response

Author

Lyu, Xiao‐Ming, Zhu, Xiao‐Wei, Zhao, Manli, Zuo, Xian‐Bo, Huang, Zhong‐Xi, Liu, Xiao, Jiang, Tao, Yang, Xue‐Xi, Li, Xin, Long, Xiao‐Bing, Wang, Jian‐Guo, Li, Jin‐Bang, Han, Ming‐Yu, Wang, Shuang, Liu, Teng‐Fei, Zhang, Bo, Sun, Tao, Cheng, Zhi, Qiu, Mo‐Chang, Dong, Lei, Zheng, Lu, Zhang, Long‐Cheng, Wang, Jia‐Hong, Wei, Gan‐Guan, Yao, Kaitai, Wang, Qian, and Zheng, Hou‐Feng

Subjects
Biomedical and Clinical Sciences, Oncology and Carcinogenesis, Dental/Oral and Craniofacial Disease, Biotechnology, Human Genome, Prevention, Genetics, 2.1 Biological and endogenous factors, Aetiology, Alleles, Case-Control Studies, Cell Line, Tumor, DNA-Binding Proteins, Female, Gene Expression Profiling, Gene Expression Regulation, Neoplastic, Genotype, High-Throughput Nucleotide Sequencing, Humans, Immunomodulation, Killer Cells, Natural, Leukocytes, Mononuclear, Male, Mutation, Nasopharyngeal Carcinoma, Neoplasm Staging, Polymorphism, Single Nucleotide, Tripartite Motif Proteins, Ubiquitin-Protein Ligases, TRIM26, immune response, nasopharyngeal carcinoma, single-nucleotide polymorphisms, targeted MHC sequencing, Biochemistry and Cell Biology, Oncology and carcinogenesis
Abstract

The major histocompatibility complex (MHC) is most closely associated with nasopharyngeal carcinoma (NPC), but the complexity of its genome structure has proven challenging for the discovery of causal MHC loci or genes. We conducted a targeted MHC sequencing in 40 Cantonese NPC patients followed by a two-stage replication in 1065 NPC cases and 2137 controls of Southern Chinese descendent. Quantitative RT-PCR analysis (qRT-PCR) was used to detect gene expression status in 108 NPC and 43 noncancerous nasopharyngeal (NP) samples. Luciferase reporter assay and chromatin immunoprecipitation (ChIP) were used to assess the transcription factor binding site. We discovered that a novel SNP rs117565607_A at TRIM26 displayed the strongest association (OR = 1.909, Pcombined = 2.750 × 10-19 ). We also observed that TRIM26 was significantly downregulated in NPC tissue samples with genotype AA/AT than TT. Immunohistochemistry (IHC) test also found the TRIM26 protein expression in NPC tissue samples with the genotype AA/AT was lower than TT. According to computational prediction, rs117565607 locus was a binding site for the transcription factor Yin Yang 1 (YY1). We observed that the luciferase activity of YY1 which is binding to the A allele of rs117565607 was suppressed. ChIP data showed that YY1 was binding with T not A allele. Significance analysis of microarray suggested that TRIM26 downregulation was related to low immune response in NPC. We have identified a novel gene TRIM26 and a novel SNP rs117565607_A associated with NPC risk by regulating transcriptional process and established a new functional link between TRIM26 downregulation and low immune response in NPC.

Published
2018

9. Supplementary Table 4 from Establishment and Characterization of an Epstein-Barr Virus–positive Cell Line from a Non-keratinizing Differentiated Primary Nasopharyngeal Carcinoma

Author

Chai, Annie Wai Yeeng, primary, Yee, Shi Mun, primary, Lee, Hui Mei, primary, Abdul Aziz, Norazlin, primary, Yee, Pei San, primary, Marzuki, Marini, primary, Wong, Ka Wo, primary, Chiang, Alan K.S., primary, Chow, Larry Ka-Yue, primary, Dai, Wei, primary, Liu, Teng Fei, primary, Tan, Lu Ping, primary, Khoo, Alan Soo Beng, primary, Lo, Kwok Wai, primary, Lim, Paul V.H., primary, Rajadurai, Pathmanathan, primary, Lightfoot, Howard, primary, Barthorpe, Syd, primary, Garnett, Mathew J., primary, and Cheong, Sok Ching, primary

Published
2024
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10. Supplementary Figure 2 from Establishment and Characterization of an Epstein-Barr Virus–positive Cell Line from a Non-keratinizing Differentiated Primary Nasopharyngeal Carcinoma

Author

Chai, Annie Wai Yeeng, primary, Yee, Shi Mun, primary, Lee, Hui Mei, primary, Abdul Aziz, Norazlin, primary, Yee, Pei San, primary, Marzuki, Marini, primary, Wong, Ka Wo, primary, Chiang, Alan K.S., primary, Chow, Larry Ka-Yue, primary, Dai, Wei, primary, Liu, Teng Fei, primary, Tan, Lu Ping, primary, Khoo, Alan Soo Beng, primary, Lo, Kwok Wai, primary, Lim, Paul V.H., primary, Rajadurai, Pathmanathan, primary, Lightfoot, Howard, primary, Barthorpe, Syd, primary, Garnett, Mathew J., primary, and Cheong, Sok Ching, primary

Published
2024
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11. Supplementary Methods from Establishment and Characterization of an Epstein-Barr Virus–positive Cell Line from a Non-keratinizing Differentiated Primary Nasopharyngeal Carcinoma

Author

Chai, Annie Wai Yeeng, primary, Yee, Shi Mun, primary, Lee, Hui Mei, primary, Abdul Aziz, Norazlin, primary, Yee, Pei San, primary, Marzuki, Marini, primary, Wong, Ka Wo, primary, Chiang, Alan K.S., primary, Chow, Larry Ka-Yue, primary, Dai, Wei, primary, Liu, Teng Fei, primary, Tan, Lu Ping, primary, Khoo, Alan Soo Beng, primary, Lo, Kwok Wai, primary, Lim, Paul V.H., primary, Rajadurai, Pathmanathan, primary, Lightfoot, Howard, primary, Barthorpe, Syd, primary, Garnett, Mathew J., primary, and Cheong, Sok Ching, primary

Published
2024
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12. Supplementary Figure 7 from Establishment and Characterization of an Epstein-Barr Virus–positive Cell Line from a Non-keratinizing Differentiated Primary Nasopharyngeal Carcinoma

Author

Chai, Annie Wai Yeeng, primary, Yee, Shi Mun, primary, Lee, Hui Mei, primary, Abdul Aziz, Norazlin, primary, Yee, Pei San, primary, Marzuki, Marini, primary, Wong, Ka Wo, primary, Chiang, Alan K.S., primary, Chow, Larry Ka-Yue, primary, Dai, Wei, primary, Liu, Teng Fei, primary, Tan, Lu Ping, primary, Khoo, Alan Soo Beng, primary, Lo, Kwok Wai, primary, Lim, Paul V.H., primary, Rajadurai, Pathmanathan, primary, Lightfoot, Howard, primary, Barthorpe, Syd, primary, Garnett, Mathew J., primary, and Cheong, Sok Ching, primary

Published
2024
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13. Supplementary Figure 6 from Establishment and Characterization of an Epstein-Barr Virus–positive Cell Line from a Non-keratinizing Differentiated Primary Nasopharyngeal Carcinoma

Author

Chai, Annie Wai Yeeng, primary, Yee, Shi Mun, primary, Lee, Hui Mei, primary, Abdul Aziz, Norazlin, primary, Yee, Pei San, primary, Marzuki, Marini, primary, Wong, Ka Wo, primary, Chiang, Alan K.S., primary, Chow, Larry Ka-Yue, primary, Dai, Wei, primary, Liu, Teng Fei, primary, Tan, Lu Ping, primary, Khoo, Alan Soo Beng, primary, Lo, Kwok Wai, primary, Lim, Paul V.H., primary, Rajadurai, Pathmanathan, primary, Lightfoot, Howard, primary, Barthorpe, Syd, primary, Garnett, Mathew J., primary, and Cheong, Sok Ching, primary

Published
2024
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14. Supplementary Figure 4 from Establishment and Characterization of an Epstein-Barr Virus–positive Cell Line from a Non-keratinizing Differentiated Primary Nasopharyngeal Carcinoma

Author

Chai, Annie Wai Yeeng, primary, Yee, Shi Mun, primary, Lee, Hui Mei, primary, Abdul Aziz, Norazlin, primary, Yee, Pei San, primary, Marzuki, Marini, primary, Wong, Ka Wo, primary, Chiang, Alan K.S., primary, Chow, Larry Ka-Yue, primary, Dai, Wei, primary, Liu, Teng Fei, primary, Tan, Lu Ping, primary, Khoo, Alan Soo Beng, primary, Lo, Kwok Wai, primary, Lim, Paul V.H., primary, Rajadurai, Pathmanathan, primary, Lightfoot, Howard, primary, Barthorpe, Syd, primary, Garnett, Mathew J., primary, and Cheong, Sok Ching, primary

Published
2024
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15. Supplementary Table 7 from Establishment and Characterization of an Epstein-Barr Virus–positive Cell Line from a Non-keratinizing Differentiated Primary Nasopharyngeal Carcinoma

Author

Chai, Annie Wai Yeeng, primary, Yee, Shi Mun, primary, Lee, Hui Mei, primary, Abdul Aziz, Norazlin, primary, Yee, Pei San, primary, Marzuki, Marini, primary, Wong, Ka Wo, primary, Chiang, Alan K.S., primary, Chow, Larry Ka-Yue, primary, Dai, Wei, primary, Liu, Teng Fei, primary, Tan, Lu Ping, primary, Khoo, Alan Soo Beng, primary, Lo, Kwok Wai, primary, Lim, Paul V.H., primary, Rajadurai, Pathmanathan, primary, Lightfoot, Howard, primary, Barthorpe, Syd, primary, Garnett, Mathew J., primary, and Cheong, Sok Ching, primary

Published
2024
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16. Supplementary Table 6 from Establishment and Characterization of an Epstein-Barr Virus–positive Cell Line from a Non-keratinizing Differentiated Primary Nasopharyngeal Carcinoma

Author

Chai, Annie Wai Yeeng, primary, Yee, Shi Mun, primary, Lee, Hui Mei, primary, Abdul Aziz, Norazlin, primary, Yee, Pei San, primary, Marzuki, Marini, primary, Wong, Ka Wo, primary, Chiang, Alan K.S., primary, Chow, Larry Ka-Yue, primary, Dai, Wei, primary, Liu, Teng Fei, primary, Tan, Lu Ping, primary, Khoo, Alan Soo Beng, primary, Lo, Kwok Wai, primary, Lim, Paul V.H., primary, Rajadurai, Pathmanathan, primary, Lightfoot, Howard, primary, Barthorpe, Syd, primary, Garnett, Mathew J., primary, and Cheong, Sok Ching, primary

Published
2024
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17. Supplementary Table 8 from Establishment and Characterization of an Epstein-Barr Virus–positive Cell Line from a Non-keratinizing Differentiated Primary Nasopharyngeal Carcinoma

Author

Chai, Annie Wai Yeeng, primary, Yee, Shi Mun, primary, Lee, Hui Mei, primary, Abdul Aziz, Norazlin, primary, Yee, Pei San, primary, Marzuki, Marini, primary, Wong, Ka Wo, primary, Chiang, Alan K.S., primary, Chow, Larry Ka-Yue, primary, Dai, Wei, primary, Liu, Teng Fei, primary, Tan, Lu Ping, primary, Khoo, Alan Soo Beng, primary, Lo, Kwok Wai, primary, Lim, Paul V.H., primary, Rajadurai, Pathmanathan, primary, Lightfoot, Howard, primary, Barthorpe, Syd, primary, Garnett, Mathew J., primary, and Cheong, Sok Ching, primary

Published
2024
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18. Supplementary Table 1 from Establishment and Characterization of an Epstein-Barr Virus–positive Cell Line from a Non-keratinizing Differentiated Primary Nasopharyngeal Carcinoma

Author

Chai, Annie Wai Yeeng, primary, Yee, Shi Mun, primary, Lee, Hui Mei, primary, Abdul Aziz, Norazlin, primary, Yee, Pei San, primary, Marzuki, Marini, primary, Wong, Ka Wo, primary, Chiang, Alan K.S., primary, Chow, Larry Ka-Yue, primary, Dai, Wei, primary, Liu, Teng Fei, primary, Tan, Lu Ping, primary, Khoo, Alan Soo Beng, primary, Lo, Kwok Wai, primary, Lim, Paul V.H., primary, Rajadurai, Pathmanathan, primary, Lightfoot, Howard, primary, Barthorpe, Syd, primary, Garnett, Mathew J., primary, and Cheong, Sok Ching, primary

Published
2024
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19. Supplementary Table 3 from Establishment and Characterization of an Epstein-Barr Virus–positive Cell Line from a Non-keratinizing Differentiated Primary Nasopharyngeal Carcinoma

Author

Chai, Annie Wai Yeeng, primary, Yee, Shi Mun, primary, Lee, Hui Mei, primary, Abdul Aziz, Norazlin, primary, Yee, Pei San, primary, Marzuki, Marini, primary, Wong, Ka Wo, primary, Chiang, Alan K.S., primary, Chow, Larry Ka-Yue, primary, Dai, Wei, primary, Liu, Teng Fei, primary, Tan, Lu Ping, primary, Khoo, Alan Soo Beng, primary, Lo, Kwok Wai, primary, Lim, Paul V.H., primary, Rajadurai, Pathmanathan, primary, Lightfoot, Howard, primary, Barthorpe, Syd, primary, Garnett, Mathew J., primary, and Cheong, Sok Ching, primary

Published
2024
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20. Supplementary Figure 3 from Establishment and Characterization of an Epstein-Barr Virus–positive Cell Line from a Non-keratinizing Differentiated Primary Nasopharyngeal Carcinoma

Author

Chai, Annie Wai Yeeng, primary, Yee, Shi Mun, primary, Lee, Hui Mei, primary, Abdul Aziz, Norazlin, primary, Yee, Pei San, primary, Marzuki, Marini, primary, Wong, Ka Wo, primary, Chiang, Alan K.S., primary, Chow, Larry Ka-Yue, primary, Dai, Wei, primary, Liu, Teng Fei, primary, Tan, Lu Ping, primary, Khoo, Alan Soo Beng, primary, Lo, Kwok Wai, primary, Lim, Paul V.H., primary, Rajadurai, Pathmanathan, primary, Lightfoot, Howard, primary, Barthorpe, Syd, primary, Garnett, Mathew J., primary, and Cheong, Sok Ching, primary

Published
2024
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21. Supplementary Figure 5 from Establishment and Characterization of an Epstein-Barr Virus–positive Cell Line from a Non-keratinizing Differentiated Primary Nasopharyngeal Carcinoma

Author

Chai, Annie Wai Yeeng, primary, Yee, Shi Mun, primary, Lee, Hui Mei, primary, Abdul Aziz, Norazlin, primary, Yee, Pei San, primary, Marzuki, Marini, primary, Wong, Ka Wo, primary, Chiang, Alan K.S., primary, Chow, Larry Ka-Yue, primary, Dai, Wei, primary, Liu, Teng Fei, primary, Tan, Lu Ping, primary, Khoo, Alan Soo Beng, primary, Lo, Kwok Wai, primary, Lim, Paul V.H., primary, Rajadurai, Pathmanathan, primary, Lightfoot, Howard, primary, Barthorpe, Syd, primary, Garnett, Mathew J., primary, and Cheong, Sok Ching, primary

Published
2024
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22. Supplementary Table 5 from Establishment and Characterization of an Epstein-Barr Virus–positive Cell Line from a Non-keratinizing Differentiated Primary Nasopharyngeal Carcinoma

Author

Chai, Annie Wai Yeeng, primary, Yee, Shi Mun, primary, Lee, Hui Mei, primary, Abdul Aziz, Norazlin, primary, Yee, Pei San, primary, Marzuki, Marini, primary, Wong, Ka Wo, primary, Chiang, Alan K.S., primary, Chow, Larry Ka-Yue, primary, Dai, Wei, primary, Liu, Teng Fei, primary, Tan, Lu Ping, primary, Khoo, Alan Soo Beng, primary, Lo, Kwok Wai, primary, Lim, Paul V.H., primary, Rajadurai, Pathmanathan, primary, Lightfoot, Howard, primary, Barthorpe, Syd, primary, Garnett, Mathew J., primary, and Cheong, Sok Ching, primary

Published
2024
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23. TABLE 1 from Establishment and Characterization of an Epstein-Barr Virus–positive Cell Line from a Non-keratinizing Differentiated Primary Nasopharyngeal Carcinoma

Author

Chai, Annie Wai Yeeng, primary, Yee, Shi Mun, primary, Lee, Hui Mei, primary, Abdul Aziz, Norazlin, primary, Yee, Pei San, primary, Marzuki, Marini, primary, Wong, Ka Wo, primary, Chiang, Alan K.S., primary, Chow, Larry Ka-Yue, primary, Dai, Wei, primary, Liu, Teng Fei, primary, Tan, Lu Ping, primary, Khoo, Alan Soo Beng, primary, Lo, Kwok Wai, primary, Lim, Paul V.H., primary, Rajadurai, Pathmanathan, primary, Lightfoot, Howard, primary, Barthorpe, Syd, primary, Garnett, Mathew J., primary, and Cheong, Sok Ching, primary

Published
2024
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24. Supplementary Table 9 from Establishment and Characterization of an Epstein-Barr Virus–positive Cell Line from a Non-keratinizing Differentiated Primary Nasopharyngeal Carcinoma

Author

Chai, Annie Wai Yeeng, primary, Yee, Shi Mun, primary, Lee, Hui Mei, primary, Abdul Aziz, Norazlin, primary, Yee, Pei San, primary, Marzuki, Marini, primary, Wong, Ka Wo, primary, Chiang, Alan K.S., primary, Chow, Larry Ka-Yue, primary, Dai, Wei, primary, Liu, Teng Fei, primary, Tan, Lu Ping, primary, Khoo, Alan Soo Beng, primary, Lo, Kwok Wai, primary, Lim, Paul V.H., primary, Rajadurai, Pathmanathan, primary, Lightfoot, Howard, primary, Barthorpe, Syd, primary, Garnett, Mathew J., primary, and Cheong, Sok Ching, primary

Published
2024
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25. Supplementary Table 2 from Establishment and Characterization of an Epstein-Barr Virus–positive Cell Line from a Non-keratinizing Differentiated Primary Nasopharyngeal Carcinoma

Author

Chai, Annie Wai Yeeng, primary, Yee, Shi Mun, primary, Lee, Hui Mei, primary, Abdul Aziz, Norazlin, primary, Yee, Pei San, primary, Marzuki, Marini, primary, Wong, Ka Wo, primary, Chiang, Alan K.S., primary, Chow, Larry Ka-Yue, primary, Dai, Wei, primary, Liu, Teng Fei, primary, Tan, Lu Ping, primary, Khoo, Alan Soo Beng, primary, Lo, Kwok Wai, primary, Lim, Paul V.H., primary, Rajadurai, Pathmanathan, primary, Lightfoot, Howard, primary, Barthorpe, Syd, primary, Garnett, Mathew J., primary, and Cheong, Sok Ching, primary

Published
2024
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26. Establishment and characterization of an Epstein-Barr virus-positive cell line from a non-keratinizing differentiated primary nasopharyngeal carcinoma

Author

Chai, Annie Wai Yeeng, primary, Yee, Shi Mun, additional, Lee, Hui Mei, additional, Abdul Aziz, Norazlin, additional, Yee, Pei San, additional, Marzuki, Marini, additional, Wong, Ka Wo, additional, Chiang, Alan K.S., additional, Chow, Larry Ka-Yue, additional, Dai, Wei, additional, Liu, Teng Fei, additional, Tan, Lu Ping, additional, Khoo, Alan Soo Beng, additional, Lo, Kwok Wai, additional, Lim, Paul VH, additional, Rajadurai, Pathmanathan, additional, Lightfoot, Howard, additional, Barthorpe, Syd, additional, Garnett, Mathew J, additional, and Cheong, Sok Ching, additional

Published
2024
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27. A data-driven method for syndrome type identification and classification in traditional Chinese medicine

Author

Zhang, Nevin L., Fu, Chen, Liu, Teng Fei, Chen, Bao Xin, Poon, Kin Man, Chen, Pei Xian, and Zhang, Yun Ling

Subjects
Computer Science - Learning, Statistics - Applications
Abstract

Objective: The efficacy of traditional Chinese medicine (TCM) treatments for Western medicine (WM) diseases relies heavily on the proper classification of patients into TCM syndrome types. We develop a data-driven method for solving the classification problem, where syndrome types are identified and quantified based on patterns detected in unlabeled symptom survey data. Method: Latent class analysis (LCA) has been applied in WM research to solve a similar problem, i.e., to identify subtypes of a patient population in the absence of a gold standard. A widely known weakness of LCA is that it makes an unrealistically strong independence assumption. We relax the assumption by first detecting symptom co-occurrence patterns from survey data and use those patterns instead of the symptoms as features for LCA. Results: The result of the investigation is a six-step method: Data collection, symptom co-occurrence pattern discovery, pattern interpretation, syndrome identification, syndrome type identification, and syndrome type classification. A software package called Lantern is developed to support the application of the method. The method is illustrated using a data set on Vascular Mild Cognitive Impairment (VMCI). Conclusions: A data-driven method for TCM syndrome identification and classification is presented. The method can be used to answer the following questions about a Western medicine disease: What TCM syndrome types are there among the patients with the disease? What is the prevalence of each syndrome type? What are the statistical characteristics of each syndrome type in terms of occurrence of symptoms? How can we determine the syndrome type(s) of a patient?

Published
2014

30. Enantioselective Formal 1,2-Diamination of Ketenes with Iminosulfinamides: Asymmetric Synthesis of Unnatural a,a-Disubstituted a-Amino Acid Derivatives

Author

Liu, Teng-Fei, Yao, Yun, and Lu, Chong-Dao

Abstract

A method was developed for the enantioselective formal 1,2-diamination of disubstituted ketenes using iminosulfinamides as nitrogen sources. The protocol involves the addition of lithium iminosulfinamides to ketenes to form N-iminosulfinyl amide metalloenolates. These metalloenolates then undergo a [2,3]-sigmatropic rearrangement to yield unnatural a,a-disubstituted a-amino acid derivatives with high enantiopurity. The chirality present at the sulfur atom in the iminosulfinamides is effectively transferred to a carbon of the resulting products, facilitating the highly enantioselective amination of ketenes.

Published
2024
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34. Transcriptome analysis reveals the regulatory role of the transcription factor StMYB113 in light-induced chlorophyll synthesis of potato tuber epidermis.

Author

ZHAO Xi-Juan, LIU Sheng-Xuan, LIU Teng-Fei, ZHENG Jie, DU Juan, HU Xin-Xi, SONG Bo-Tao, and HE Chang-Zheng

Abstract

Light-induced greening of tubers seriously affects its safety and economic benefits, but the mechanism of light-induced chlorophyll synthesis in potato tubers remains unclear. In this study, the related metabolites of potato tubers with different light durations were analyzed. The results were as follows: When the light duration prolonged, the chlorophyll content of the tubers gradually increased. Moreover, the chlorophyll content increased significantly at 36 hours, with which the tuber skin turned green obviously. Transcriptome sequencing and bioinformatics analysis were carried out on the samples taken at 0 hour, 6 hours, and 36 hours, and 5646 differentially expressed genes (DEGs) were identified. According to the co-expression cluster analysis and quantitative RT-PCR verification results, 9 major structure genes of chlorophyll biosynthesis pathway (StGAS1, StCHLD, StCrd1, StHEMA, StGUN4, StPORA, StUROD, StCHLM, and StCHLG) and 6 transcription factors (StSBP, StLSD, StGATA, StWRKY, StMYB-like, and StMYB113) were significant induced. Predictions of the cis-acting elements of the promoter sequences of these 9 structural genes indicated that they all contained multiple MYB binding sites. Promoter element analysis and transcriptional activation validation further revealed that StMYB113 had a light-responsive element and could activate the expression of StUROD in tobacco. Therefore, StMYB113 may be light-responsive and be able to regulate potato tuber greening in the light. This study provides a reference for the research on the regulatory mechanism of light-induced chlorophyll synthesis in potato tubers, which is important for reducing the loss caused by greening of potato tubers. [ABSTRACT FROM AUTHOR]

Published
2023
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