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In order to evaluate the status of heavy metal pollution on marine economic shellfish in eastern Guangdong and the intake of health risk, the contents of lead(Pb), cadmium(Cd), chromium(Cr), copper(Cu), zinc(Zn) and manganese(Mn) in muscle and visceral mass from 10 species of shellfish were determined by using method of inductively coupled plasma atomic emission spectrometry(ICPAES). The single factor pollution index(Pi), target hazard quotient(THQ) and the total target hazard quotient(TTHQ) were used to evaluate the pollution status of heavy metals and dietary health risk. Results showed that the levels of metals in muscles of the shellfish were found in order of Zn > Mn > Cu > Cr > Cd > Pb, while which in visceral mass were in order of Zn > Mn > Cu > Pb > Cr > Cd. Except Cr, the heavy metals concentrations in visceral mass samples were significantly higher than those in the corresponding muscles. Based on the Pi indexes, the shellfish were polluted to one degree or another by Cd, Pb, Cr and Zn, except the Pi indexes of Cu. The shellfish Pi index from high to low was in order of Cd > Zn > Pb > Cr > Cu, While the Pi index of Cd, Zn and Pb in most shellfish viscera were in heavy pollution level. The results of THQ showed that the indexes exposure to individual metal(Cd, Zn, Pb, Cr, Cu, respectively) were at none risk level for children and adults except the index of Cd in visceral mass of Pinna pectinata. However, TTHQ results showed that the TTHQ value in about half of the shellfish species were more than 1, which indicated that long-term consumption of the visceral mass of these species would have a negative impact on human health. In summary, the shellfishes were polluted by Zn, Cd, Pb and Cr to some extent, which indicated that hidden danger of heavy metals pollution was present in ecological environment and fishery production in the area, and the monitoring of heavy metal residue in shellfish and heavy metals pollution need to be strengthened.

We are aimed to observe the effect of applying the combined temporo-occipital fascial flap in the medium scalp defect with bone exposure. Three cases of moderate scalp defect with bone exposure were admitted by The 89th Hospital of PLA and China-Japan Friendship Hospital from October 2009 to March 2014, and the wounds were repaired by application of the temporo-occipital fascial flap with medium-thickness skin grafting. And then these 3 patients were followed up after the operation, and the wound repair was observed. These 3 cases of fascial flaps all survived well with good appearance and covered the wound completely. Fibrosarcoma of one case had a relapse 3 months after operation, and the other two cases were followed up from 6 months to 3 years. Meanwhile, the appearance and function were satisfactory. The communicating branches between superficial temporal artery and occipital artery are rich. Therefore we designed and utilized the long temporo-occipital fascial flap containing the ipsilateral occipital superficial fascia to repair the scalp defect with bone exposure, and the curative effect is satisfactory.

FOXL2 is a transcription factor that is essential for ovarian function and maintenance, the germline mutations of which give rise to the blepharophimosis ptosis epicanthus inversus syndrome (BPES), often associated with premature ovarian failure. Recently, its mutations have been found in ovarian granulosa cell tumors (OGCTs). In this study, we measured the expression of FOXL2 in cervical cancer by immunohistochemistry and its mRNA level in cervical cancer cell lines Hela and Siha by RT-PCR. Then we overexpressed FOXL2 in Hela cells and silenced it in Siha cells by plasmid transfection and verified using western blotting. When FOXL2 was overexpressed or silenced, cells proliferation and apoptosis were determined by Brdu assay and Annexin V/PI detection kit, respectively. In addition, we investigated the effects of FOXL2 on the adhesion and invasion of Hela and Siha cells. Finally, we analyzed the influences of FOXL2 on Ki67, PCNA and FasL by flow cytometry. The results showed that FOXL2 was highly expressed in cervical squamous cancer. Overexpressing FOXL2 suppressed Hela proliferation and facilitated its apoptosis. Silencing FOXL2 enhanced Siha proliferation and inhibited its apoptosis. Meanwhile, silencing FOXL2 promoted Siha invasion, but it had no effect on cells adhesion. In addition, overexpressing FOXL2 decreased the expression of Ki67 in Hela and Siha cells. Therefore, our results suggested that FOXL2 restrained cells proliferation and enhanced cells apoptosis mainly through decreasing Ki67 expression.