15 results on '"Liu, Ying-Ke"'
Search Results
2. Coating material of air sealing in coal mine: Clay composite slurry (CCS)
- Author
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Zhou, Fu-bao, Shi, Bo-bo, Liu, Ying-ke, Song, Xiao-lin, Cheng, Jian-wei, and Hu, Sheng-yong
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- 2013
- Full Text
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3. The effect of polyethylene glycol recombinant human growth hormone on growth and glucose metabolism in hypophysectomized rats
- Author
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Zhang, Zhi Xin, Liu, Ying Ke, Pan, Hui, Pan, Lin, Zhang, Qiong, Su, Hui Min, Zhao, Qiu Ling, Li, Hong, and He, Chun
- Published
- 2012
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4. Gas drainage efficiency: an input–output model for evaluating gas drainage projects
- Author
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Zhou, Fu-bao, Wang, Xin-xin, and Liu, Ying-ke
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- 2014
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5. Effect of adsorption-induced matrix swelling on coal permeability evolution of micro-fracture with the real geometry
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Wei, Ming-Yao, primary, Liu, Jishan, additional, Liu, Ying-Ke, additional, Liu, Zhang-Hao, additional, and Elsworth, Derek, additional
- Published
- 2021
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6. Analysis of drilling failure in soft and hard sandwiching of coal seam
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Zhou Fu-bao, Liu Ying-ke, Man Zhongyi, and Liu Chun
- Subjects
Materials science ,Drill ,Scanning electron microscope ,business.industry ,020502 materials ,Metallurgy ,General Engineering ,Coal mining ,Borehole ,Drilling ,Diamond ,Wear coefficient ,02 engineering and technology ,engineering.material ,021001 nanoscience & nanotechnology ,Abrasion (geology) ,0205 materials engineering ,engineering ,General Materials Science ,Composite material ,0210 nano-technology ,business - Abstract
The severe abrasion of drill bits will be confronted during drilling in soft and hard sandwiching of coal seam. Not only does this failure result in the increasing consumption of bits, but also it delays the construction period, significantly extending the duration of the delay, even causing borehole instability, resulting in a catastrophic buried well accident. In order to reveal the failure causes, the authors of this paper researched the wear properties of the tooth of bit. As the thermal damage is one of the main threats to drilling failure in soft and hard sandwiching, thermal related effects on the wear performance of polycrystalline diamond compacts (PCD) were studied by an Amsler friction and wear testing machine. Meanwhile, silica sands were added to the interface of wear couples throw a funnel instrument to meet the demand that wear conditions correspond more closely to those in actual coal seam drilling. The friction coefficient and wear rates was measured. The X-ray diffraction analysis was used to investigate the chemical composition and crystal structure of the PCDs soaked at different temperatures. The morphologies of the surface of PCDs were examined by metalloscope and scanning electron microscopy. The results show that the wear rate and wear coefficient all rose with the increase of wear load. So the drilling load over proper operation limits especially when the equipment stuck happened is the primary threat to bit failure. On the other hand, the wear coefficient is not sensitive to the soaking temperature when it is under 700 °C. But it decreases obviously when the temperature was over 800 °C, which seems to be corresponding to the graphitization of diamond and the oxidation of Co. This phenomenon indicates that the high temperature over 700 °C is the main cause of the drilling failure.
- Published
- 2016
7. Cuffless blood-pressure estimation method using a heart-rate variability-derived parameter
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Chen, Yang, primary, Shi, Shuo, additional, Liu, Ying-Ke, additional, Huang, Shou-Lin, additional, and Ma, Ting, additional
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- 2018
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8. Topical Sunitinib ointment alleviates Psoriasis-like inflammation by inhibiting the proliferation and apoptosis of keratinocytes
- Author
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Kuang, Ye-Hong, primary, Lu, Yan, additional, Liu, Ying-Ke, additional, Liao, Li-Qiu, additional, Zhou, Xing-Chen, additional, Qin, Qun-Shi, additional, Jia, Xue-Kun, additional, Wu, Li-Sha, additional, Zhu, Wu, additional, and Chen, Xiang, additional
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- 2018
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9. An experimental and numerical investigation on the deformation of overlying coal seams above double-seam extraction for controlling coal mine methane emissions
- Author
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Liu Lang, Zhou Fu-bao, Liu Chun, Liu Ying-ke, and Hu Shen-yong
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animal structures ,business.industry ,Stratigraphy ,ComputingMethodologies_IMAGEPROCESSINGANDCOMPUTERVISION ,Borehole ,Coal mining ,Drilling ,Geology ,Deformation (meteorology) ,Methane ,chemistry.chemical_compound ,Fuel Technology ,chemistry ,Mining engineering ,embryonic structures ,Economic Geology ,Extraction (military) ,Double seam ,Problems in coal mining ,business - Abstract
This study investigates the deformation and the resultant pressure relief of highly gassy coal seams where a double seam mining operation takes place at lower depths at the Wulan Coal Mine, China. In order to predict the depressurization effect in the overlying coal seams, we simulate the extraction process by constructing a scaled model in the laboratory using similar rock materials. Analyses of experimental results concluded that due to the mining-induced stress redistribution, the pressure within target coal seams, which were 109 m above the mined seams, could be fully relieved to attain the statutory approval for gas drainage. In addition to scaled-model studies, computational modeling studies were conducted using UDEC (Universal Distinct Element Code), which showed that the features of deformation resulting from the double-seam extraction were different from that of in the case of single-seam extraction. The results of the numerical studies revealed that in addition to the panel margin on the air return side, areas near the intake entry could also be considered as borehole drilling positions. Besides, it was found that the gob of the extracted seams below the gassy one provided a “buffering effect” for the would-mined protective coal seam and that the depressurization effect was largely weakened. The laboratorial findings are instructive to the field practice of methane drainage. During the mining operation, a “displacement comparison method” was adopted to measure the dilated amount of protected coal seams, and as expected, the maximum dilation percentage was much more than 0.2% — the critical value upon which the target coal seam is appropriately depressurized to allow gas drainage to be safely and effectively implemented.
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- 2011
10. Deformation of Overlong Isolated Buildings Caused by Thermal and Concrete Shrinkage
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Dang, Yu, primary and Liu, Ying-ke, additional
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- 2013
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11. Study on the depressurization effect produced by exploitation of lower distant protective coal seam and elimination of outburst hazard of the protected seams by applying gas drainage with surface boreholes.
- Author
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Liu Ying-ke
- Subjects
- *
COAL mining , *HAZARDS , *MINE safety , *BOREHOLE mining - Abstract
In Western China, there are some mining areas with complex geological structure and significant hazards of gas, coal and gas outburst. The outburst hazard not only threatens safety of mine gravely, but also makes it difficult to explore coal resources in some regions. Aiming at the characteristics of the complex structure, poor coal seam permeability and multi-seam mining in the mining area, with the purpose of eliminating the danger of outburst and decreasing the gas content of coal seam, this paper made a systematic research on the mining of the lower distant protective layer, the technology of pressure-relieved gas drainage using surface boreholes and eliminating the potential dangers of coal and gas outburst. By constructed the model of similar materials experiments of stope and numerical simulation, the author studied the depressurization effect of protected coal seam under the mining condition of lower distant protective layer: (1) During extraction of the lower distant layer, the stress of protected layer could be reduced sufficiently, and gas permeability coefficient could be increased substantially, providing favorable condition for gas drainage; (2) As the double protective seam mining,40-50 m area of intake entry and return entry of mining face in protected coal seams is dilated area, which is the proper location of surface boreholes; (3) Repeat depressurization mode is found that the gob of the extracted seam below the gassy one provides a "Buffering Effect" for the overlying coal seam which makes the depressurization of the overlying seam weaken. The results from mechanical model for analysis of the stability of surface borehole are: (1) The greater the difference between the strength of adjacent strata is, the bigger the squeezing and shearing stress in the casing become; (2) Under the condition of leaving the space between the casing and borehole wall, the max shearing stress of casing decreased by 50.8% evenly, and the probability of failure of casing was reduced significantly; (3) The longer the production casing is, the bigger the deflection value is and the greater anti-bending ability the casing gets. Based on the above results, the author designed a structure of surface borehole against shearing and rupturing. There leaves the "buffer spacing" between the cementing casing or borehole wall and the production casing which is whole-tube and screen section was handled by "casing enhancement technology", effectively improving the stability of surface borehole. The law of pressure relief methane drainage using surface borehole is demonstrated: (1) Illustrated the distance from working face to borehole (showing the methane permeability coefficients of overlying coal seam and fractured zone), is the key influencing factor of gas production rate of borehole; (2) Revealed the changing mechanism of "fast increase-slowly decrease" of the gas production rate of borehole during the protective coal seam mining, and determined the best layout parameters of surface boreholes; (3) Built a mathematical model for determining the gas flow rate of overlying coal seam and gob, which can provide a basis of assessment for gas drainage of overlying coal seam; (4) Proposed the method of improving gas drainage form overlying pressure released seams with increasing the lower borehole resistance. The research results mentioned above were carried out in Wulan Coal Mine of Shenhua Group, which show that the effect of eliminating outburst by extracting pressure relieved gas with surface boreholes is remarkable. The pure gas drainage quantity of boreholes in the testing area has totally reached 1 512.96 x 104 m³. The max spacing of borehole near the intake entry and the return entry are 150 m and 169 m respectively. The remaining gas quantities of the protected coal seams are eliminated to 3.63 m³/t and 3.14 m³/t, and the gas drainage rates are 65.8% and 68.0%, respectively, completely eliminating the outburst danger of the coal. [ABSTRACT FROM AUTHOR]
- Published
- 2012
12. [Microassay of High-Risk Human Papillomavirus Genotype Based on R6G-ddATP/SNaPshot-Gel Fluorescence Method].
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Feng CR, Yang S, Wang YQ, Liu YK, Chen XY, Zeng SS, Luo YX, and Gao XF
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- DNA, Viral genetics, Deoxyadenine Nucleotides, Dideoxynucleotides, Genotype, Humans, Polymerase Chain Reaction, Alphapapillomavirus, Papillomaviridae genetics, Papillomavirus Infections
- Abstract
Objective: R6G-ddATP was used as a dideoxy fluorescence substrate to establish the single base end extension (SNaPShot)-gel fluorescence method for the rapid detection of the genotypes of three high-risk human papillomaviruses (HR-HPV) ( HPV 18, HPV 33 and HPV 35) genotypes., Methods: HPV quality control products were used as as samples, and R6G-ddATP dideoxy fluorescence reagent was used as substrate. Firstly, HPV was amplified by using universal primers to obtain the first round of amplified products, which were purified and used as templates for subsequent SNaPShot reactions. Then, specific one-step extension primers were used to perform SNaPShot reaction to generate R6G-fluorescence-labeled DNA extension products. The product was subjected to agarose gel electrophoresis, the results of which were observed under a Gel Imager, and the HPV genotyping was done with different one-step extension primers. Each sample was tested three times and the results were compared with DNA sequencing results., Results: The preferred annealing temperature for SNaPShot reaction is 55 ℃. Three HPV genotypes were examined by R6G-ddATP/SNaPShot gel fluorescence assay under optimal conditions, and the results were consistent with DNA sequencing results., Conclusion: The R6G-ddATP/SNaPShot-gel fluorescence method for the micro-detection methods of three HR-HPV genotypes was successfully established and can be used for rapid detection of HPV genotypes., (Copyright© by Editorial Board of Journal of Sichuan University (Medical Sciences).)
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- 2021
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13. [Expression and significance of insulin-like growth factor-1 and insulin-like growth factor binding protein-3 in hepatocyte steatosis model].
- Author
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Zhang Q, Zhang ZX, Fang Q, Gao FY, Zhao QL, Yang Y, Su HM, and Liu YK
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- Cell Line, Fatty Liver pathology, Hepatocytes pathology, Humans, Insulin-Like Growth Factor Binding Protein 3 genetics, Insulin-Like Growth Factor I genetics, Non-alcoholic Fatty Liver Disease, Fatty Liver metabolism, Hepatocytes metabolism, Insulin-Like Growth Factor Binding Protein 3 metabolism, Insulin-Like Growth Factor I metabolism
- Abstract
Objective: To investigate the expression of IGF-1 and IGFBP-3 in nonalcoholic fatty steatosis hepatocyte models induced by oleic acid., Method: Nonalcoholic fatty steatosis hepatocyte models induced by oleic acid on immortalized human hepatocyte, Oil red O staining and intracellular triglycerides were detected for observing the situation of IHH cells fatty degeneration. IHH cells were divided into control group, NAFLD group, which the control group cultured in DMEM/F12 medium, NAFLD group were treated with oleic acid, 0.5 mmol/L treatment for 72 h. The expression of mRNA and protein of IGF-1 and IGFBP3 were measured by immunofluorescent staining, Western blot and RT-PCR methods. Between the two groups were compared using the t- test., Results: The steatosis models of the hepatocytes were established successfully with 0.5 mmol/L oleic acid. Lipid droplets were observed through Oil red O staining. The level of hepatocyte TG was increased (275.7+/-27.2) mug/mg from (150.2+/-15.6) mug/mg (t = 21.67, P less than 0.01). Compared with the control group, the mRNA of IGF-1 (0.76+/-0.04 vs 4.82+/-1.51, t = 17.915, P less than 0.01), IGFBP-3 (1.58+/-0.93 vs 5.41+/-1.37, t = 12.893, P less than 0.01) and protein expression of IGF-1 (1.00+/-0.29 vs 2.56+/-0.71, t = 29.17, P less than 0.01), IGFBP-3 (0.65+/-0.36 vs 1.23+/-0.91, t = 32. 12, P less than 0.01) significantly decreased in oleic acid-treated group. The results of immunofluorescence staining also confirm the significantly decreased protein expression of IGF-1 and IGFBP-3 in NAFLD group compared with control group., Conclusion: The expression of IGF-1 and IGFBP-3 decreased in nonalcoholic fatty steatosis hepatocyte models, which will provide the experimental basis for the further study of the mechanism of the limited height of some children with non-alcoholic fatty liver disease in clinical.
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- 2012
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14. [Effect of growth hormone combined with Radix Dipsaci on the body growth and the bone metabolism of hypophysectomized rats].
- Author
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Liu YK, Zhang ZX, and Zhang Q
- Subjects
- Animals, Dipsacaceae chemistry, Hypophysectomy, Male, Rats, Rats, Sprague-Dawley, Bone Development drug effects, Bone and Bones metabolism, Drugs, Chinese Herbal pharmacology, Growth Hormone pharmacology
- Abstract
Objective: To study the effect of growth hormone (GH) combined with Radix Dipsaci on the body growth and the bone mineral content (BMC) of hypophysectomized rats., Methods: The GH deficiency rats model was established using the hypophysectomized operation through the skull and the throat. Qualified rats were divided into the sham-operation group (n = 15), the negative control group (n = 13), the GH intervention group (n = 13), and the GH combined with Radix Dipsaci group(n = 12). GH (0.25 mg/kg) was subcutaneously injected from the cervical part in the GH intervention group and the GH combined with Radix Dipsaci group at the same time, while equal volume of normal saline was injected to the rest groups. 0.7 mL/100 kg Radix Dipsaci was given by gastrogavage to the GH combined with Radix Dipsaci group at the same time, while equal volume of normal saline was given by gastrogave to the rest groups. The body weight, the tail length, and the body length were measured during the intervention period. Blood was withdrawn after 14-day intervention. The femoral bone and the tibial bone were taken out. The levels of GH, insulin-like growth factor 1 (IGF-1), alkaline phosphatase (ALP), and osteocalcin (OC) were measured. The width of the tibial epiphyseal plate was measured. The bilateral femur bone mineral density (BMD) and BMC were measured using the dual energy X-ray absorptiometry., Results: The body weight, the body length, the length of the femoral bone, the length of the tibial bone, the width of the epiphyseal plate, the levels of the GH, IGF-1, ALP, and OC increased in the GH intervention group and the GH combined with Radix Dipsaci group after 2-week intervention, showing statistical difference when compared with the model group (P < 0.01). But there was no statistical difference in the tail length though it also increased (P > 0.05). There was insignificant difference in the aforesaid indices between the two groups (P > 0.05). Compared with the model group, the BMD of the GH combined with Radix Dipsaci group increased with statistical difference (P < 0.01). Compared with the model group, the BMC of the GH intervention group and the GH combined with Radix Dipsaci group increased with statistical difference (P < 0.01). It was highest in the GH combined with Radix Dipsaci group (P < 0.01)., Conclusions: GH combined with Radix Dipsaci showed unobvious effect on promoting the growth. But it could elevate BMD and BMC, and improve the bone metabolism.
- Published
- 2011
15. [Metabolic characteristics of a fatty liver disease model induced by high-fat feeding in young rats].
- Author
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Su HM, Zhang ZX, Pan L, Guo YR, Liu YK, and Zhang Q
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- Animals, Blood Glucose metabolism, Body Mass Index, Cholesterol blood, Dietary Fats administration & dosage, Fatty Liver blood, Fatty Liver etiology, Fatty Liver pathology, Female, Immunohistochemistry, Insulin Resistance, Leptin metabolism, Liver pathology, Male, Non-alcoholic Fatty Liver Disease, Random Allocation, Rats, Rats, Sprague-Dawley, Triglycerides blood, Disease Models, Animal, Insulin blood, Liver metabolism, Sterol Regulatory Element Binding Protein 1 metabolism
- Abstract
Objective: To establish nonalcoholic fatty liver disease (NAFLD) in young rats, and to investigate the metabolic characteristics of these rats., Methods: Fifteen male and fifteen female SD rats of 3 weeks old were randomly divided into three groups, normal group (N), 20% high fat group (HF1) and 30% high fat group (HF2). All the rats were fed under Specific pathogen Free (SPF) condition for 6 weeks and executed at the end of the 6th week. Body length and weight of each rat as well as their liver weight were measured for calculating Liver Index (LI). ALT, AST, TG, TC, INS, Glu and HOMA-IR in the blood were measured. Liver tissue homogenate was prepared for detecting TG level. The liver section was stained with HE and oil red. The expression of SPEBP-1 and leptin in liver was detected by immunostaining., Results: The typical pathological change of NAFLD was found in the rats of HF groups. In HF2 group, no rats died during the experiment and the degree of fat degeneration is homogeneous. Comparing with those in N group, TC (mmol/L), liver TG (mmol/L) and ALT levels in HF2 group were significantly elevated (2.50+/-0.39 vs 1.82+/-0.43, P less than 0.01; 25.38+/-13.29 vs 12.09+/-9.59, P less than 0.01 and 69.80+/-18.22 vs 48.00+/-10.45, P less than 0.01, respectively). Comparing with those in N group, TG level in HF1 group was significantly decreased (0.17+/-0.10 vs 0.32+/-0.12, P less than 0.05), Glu level in HF1 group was significantly elevated (12.33+/-3.48 vs 8.13+/-2.53, P less than 0.05). There were no significant difference between the results of AST, INS and HOMA-IR among the groups. The expression level of SREBP-1 and leptin increased in HF groups., Conclusion: NAFLD can be induced by 30% high-fat feeding for 6 weeks in young rats, high-fat feeding induces the expression of SREBP-1 and leptin expression and fat synthesis.
- Published
- 2010
- Full Text
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