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Additional file 1: Figure S1. Screening β-farnesene synthase from different plants. A The evolutionary tree of β-farnesene synthase from different plants. B β-Farnesene production and biomass of strains containing different β-farnesene synthase. Data represent the mean ± SD of biological triplicate. Figure S2. The SDS-PAGE electrophoresis analysis of purified protein AanFSK197T/F180H and AanFS. Figure S3. The copy number and relative expression of AanFSK197T/F180H in Q6 and Q7 strains. Data represent the mean ± SD of biological duplicate. Figure S4. Intracellular lipids accumulation in PO1f, CP7 and Q12 strain. Data represent the mean ± SD of biological triplicate. Figure S5. Fermentation conditions optimization of Q26 strain in 5 L bioreactor. A β-Farnesene production at different air flux and stirring rate. B Growth curve at different air flux and stirring rate during fermentation. C β-Farnesene production at different pH. D Growth curve at different pH. β-Farnesene detected after 96 h of fermentation. Data represent the mean ± SD of biological duplicate. Figure S6. The relationship of the engineered strains used in this study. CP7 strain was obtained by locating the synthesis pathway of mevalonate in the peroxisome of AHH12 strain. Different genes of MVA pathway were successively overexpressed in CP7 strain to obtain Q3–Q7 strains, respectively. On the basis of strain Q7, the genes involved in β-oxidation were overexpressed, respectively, to construct strain Q8–Q14, and Q17–Q21 strain was obtained by overexpressing other genes related to β-oxidation basing on Q12 strain. Q15 strain was constructed by regulating citric acid metabolism of Q7 strain and Q16 strain was derived from Q15 strain. Expressing citric acid regulating genes in Q12 strain obtained Q22 strain. On the basis of strain Q12, the fatty acid anabolism was regulated to construct Q23–Q26 strain. On the basis of strain Q26, the combinatorial regulation of fatty acid anabolism obtained Q27 strain. Table S1. The primary primers used in this study. Table S2. The codon optimized sequences of β-farnesene synthase from different plants. Table S3. The detailed information of genes used in this study. Table S4. The accumulation of by-products at 216 h of fermentation. Data represent the mean ± SD of biological duplicate.

Additional file 1: Table S1. Strains used in this study. Table S2. Plasmids used in this study. Table S3. Primers used in this study. Table S4. Encoding sequences of C4-dicarboxylic acid transporters from different species. Figure S1. CRISPR interference (CRISPRi) system in Y. lipolytica. (a) Episomal vector pCRISPRi for the simultaneous expression of dCas9 and sgRNA; (b) functional verification of CRISPRi using hrGFP as the reporter gene. 4 different sgRNA targeting sites were designed in the front part of the hrGFP gene, its relative expression level could be as low as 27.6% of the control. Figure S2. Screening of transformants with overexpressed YlScs2 (a) and TbFrd (b) through NHEJ-mediated random genome integration for SA production. Figure S3. Comparison of the SA titer and yield between different Y. lipolytica engineered strains after 72 h fermentation in shaking flasks.

Additional file 1: Figure S1. α-Farnesene, mevalonate accumulation and OD600 of recombinant strains randomly selected from F9 library. Data were detected after 96 h of fermentation in 300-mL shaken flasks containing 50 mL YP medium with oleic acid. Date of transformants were performed with n = 1, while date of F5 strain represent the mean±SD of biological triplicates. Figure S2. Optimization of medium for fermentation of F10 strain in shake flasks. a Initial concentration of oleic acid. b Concentration of Tween 80 (‰). c Different cations. All data represents the mean ± SD of biological triplicates. Figure S3. The mevalonate and by-products production in 5 L fermentor under the optimum conditions of F10 strain. The error bars represent standard deviations of duplicate cultivations. Figure S4. α-Farnesene production on WCO and different oil substrates. a α-Farnesene, mevalonate accumulation and OD600 of F10 strain under different WCO initial addition at 96 h. b α-Farnesene accumulation and OD600 of F10 strain on different oil types at 96 h. Data represents the mean ± SD of biological triplicates. Table S1. Primers used in this study.

A total of 332 sugarcane leaf samples were collected from seven provinces in China during 2010 to 2013. RT-PCR was performed using the YLS111/YLS462 primers to detect the causal pathogen Sugarcane yellow leaf virus (SCYLV) causing yellow leaf disease in sugarcane. The incidence of SCYLV infection ranged from 24 to 38 % depending on geographic areas. A new primer pair (P0-R3/P0-F2) was designed to prime the amplification of a 968-nucleotide (nt) fragment from SCYLV-positive samples. The fragment contained a partial 5′ untranslated region, the complete ORF0 encoding P0, and a partial ORF1. A phylogenetic analysis of 141 P0 fragment sequences (937 nt) worldwide showed that all SCYLV isolates were clustered into eight genotypes. The 107 SCYLV isolates collected in this study were classified into three genotypes (BRA, HAW, and CHN3), of which the BRA genotype (102/107) was the most prevalent and was detected in all evaluated locations. One isolate (1/107) of genotype HAW was found in Hainan province. Four isolates (4/107) of the genotype CHN3 were observed in Guangxi, Guizhou and Hainan provinces. These findings help understand the genotypic diversity and distribution of SCYLV isolates in China.