1. Effects of Tween 80 and pH on mycelial pellets and exopolysaccharide production in liquid culture of a medicinal fungus.
- Author
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Liu, Yuan-Shuai and Wu, Jian-Yong
- Subjects
- *
MICROBIAL exopolysaccharides , *BIOSYNTHESIS , *PH effect , *NITROGEN , *FUNGAL cultures , *FUNGAL cell walls ,THERAPEUTIC use of fungi - Abstract
This study investigated the effects of surfactant additives and medium pH on mycelial morphology and exopolysaccharide (EPS) production in liquid culture of a valuable medicinal fungus Cordyceps sinensis Cs-HK1. In the medium containing 20 g l glucose and 6 g l peptone as the sole nitrogen source, the Cs-HK1 fungal mycelia formed smooth and spherical pellets about 1.8-mm mean diameter. The mycelial pellets became less uniform at pH (4.0-5.0) lower than the optimum (pH 6.0) or turned to filamentous form at higher pH (8-9). Surfactants added to the medium inhibited pellet formation, resulting in smaller and looser pellets. Tween 80 exhibited a remarkable promoting effect on EPS production, increasing the EPS yield more than twofold at 1.5% (w/v), which was most probably attributed to the stimulation of EPS biosynthesis and release from the fungal cells by Tween 80. [ABSTRACT FROM AUTHOR]
- Published
- 2012
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