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158 results on '"Liu, Zengping"'

3. Prognosis and influencing factors of 30 cases of malignant melanoma of nasal cavity and paranasal sinuses

Author

Shi Yuanzhen, Hou Yuan, Liu Longyan, Li Yong, Liu Zengping, Wang Yanli, and Duan Shihong

Subjects
melanoma, nasal cavity, sinus tumor, prognosis, Medicine, Biotechnology, TP248.13-248.65
Abstract

Objective To investigate the clinical features of malignant melanoma of nasal cavity and paranasal sinuses (sinonasal malignant melanoma, SNMM) and its correlation with prognosis. Methods A retrospective analysis was conducted on 30 cases of SNMM treated at the Second Hospital of Lanzhou University from July 2010 to March 2024. Kaplan-Meier method and Cox regression model were used for survival analysis to identify factors affecting patient prognosis.Results Among the 30 patients,15 were males and 15 were females,aged between 38 and 81 years with an average age of 60.37 years.Survival time ranged from 1 to 89 months,with a 1-year survival rate of 81.00%, 3-year survival rate of 54.09%, and 5-year survival rate of 37.86%.The median survival time was 28.13 months. Kaplan-Meier method suggested that age, time from initial symptoms to diagnosis, primary tumor site, orbital involvement, Ki-67 expression levels, tumor staging and treatment were associated with survival rates.Tumor staging was identified as an independent factor affecting the survival rate in SNMM patients. Conclusion SNMM has high malignancy and poor prognosis, with regular postoperative followup being critical to improving survival rates. Current treatment mainly consists of a combination of surgery,postoperative radiotherapy,chemotherapy,immunotherapy and biotherapy.

Published
2024
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5. A Novel Mutation Located in the N‐Terminal Domain of MYO15A Caused Sensorineural Hearing Loss.

Author

Wang, Yanli, Liu, Zengping, Li, Yong, Nie, Zhipeng, Xu, Baicheng, Zhu, Yiming, Duan, Shihong, Chen, Xingjian, Tan, Huan, Dang, Jiong, Guan, Minxin, and Guo, Yufen

Subjects
*PLURIPOTENT stem cells, *HAIR cells, *SENSORINEURAL hearing loss, *INNER ear, *HEARING disorders, *RECESSIVE genes
Abstract

Background: MYO15A is one of the common genes of severe‐to‐profound sensorineural deafness. Mutations in this gene can cause both pre‐ and post‐lingual hearing losses. In this study, a novel MYO15A variant (c.2482C>T) was identified to be associated with autosomal recessive non‐syndromic hearing loss (ARNSHL) in a Chinese Uighur family. Methods: To examine the effects of the MYO15A mutation on the morphology and function of the derived hair cell‐like cells, two iPSCs were generated separately from the proband and a mutation‐negative family member and those were then induced to hair cell‐like cells. Results: Results showed that this homozygous MYO15A mutation (PVS1 + PM2 + PP1 + PP3), which is located in the N‐terminal domain, displayed significant differences in the morphology and function of hair cell‐like cells between the proband and the normal control, although it had no effect on the totipotency of iPSCs. Conclusion: Our study demonstrates that the novel variant c.2482C>T in the MYO15A gene may cause inner ear hair cell dysfunction and audiological disorders in this family. [ABSTRACT FROM AUTHOR]

Published
2024
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8. A bio-functional polymer that prevents retinal scarring through modulation of NRF2 signalling pathway

Author

Parikh, Bhav Harshad, Liu, Zengping, Blakeley, Paul, Lin, Qianyu, Singh, Malay, Ong, Jun Yi, Ho, Kim Han, Lai, Joel Weijia, Bogireddi, Hanumakumar, Tran, Kim Chi, Lim, Jason Y. C., Xue, Kun, Al-Mubaarak, Abdurrahmaan, Yang, Binxia, R, Sowmiya, Regha, Kakkad, Wong, Daniel Soo Lin, Tan, Queenie Shu Woon, Zhang, Zhongxing, Jeyasekharan, Anand D., Barathi, Veluchamy Amutha, Yu, Weimiao, Cheong, Kang Hao, Blenkinsop, Timothy A., Hunziker, Walter, Lingam, Gopal, Loh, Xian Jun, and Su, Xinyi

Published
2022
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13. Surgical Approaches for Cell Therapeutics Delivery to the Retinal Pigment Epithelium and Retina

Author

Stanzel, Boris, Ader, Marius, Liu, Zengping, Amaral, Juan, Aguirre, Luis Ignacio Reyes, Rickmann, Annekatrin, Barathi, Veluchamy A., Tan, Gavin S. W., Degreif, Andrea, Al-Nawaiseh, Sami, Szurman, Peter, Cohen, Irun R., Editorial Board Member, Lajtha, Abel, Editorial Board Member, Lambris, John D., Series Editor, Paoletti, Rodolfo, Editorial Board Member, Rezaei, Nima, Series Editor, and Bharti, Kapil, editor

Published
2019
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16. Retinal-detachment repair and vitreous-like-body reformation via a thermogelling polymer endotamponade

Author

Liu, Zengping, Liow, Sing Shy, Lai, Siew Li, Alli-Shaik, Asfa, Holder, Graham E., Parikh, Bhav Harshad, Krishnakumar, Subramanian, Li, Zibiao, Tan, Mein Jin, Gunaratne, Jayantha, Barathi, Veluchamy Amutha, Hunziker, Walter, Lakshminarayanan, Rajamani, Tan, Clement Woon Teck, Chee, Caroline K., Zhao, Paul, Lingam, Gopal, Loh, Xian Jun, and Su, Xinyi

Published
2019
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19. Single-cell transcriptomics reveals maturation of transplanted stem cell–derived retinal pigment epithelial cells toward native state

Author

Parikh, Bhav Harshad, primary, Blakeley, Paul, additional, Regha, Kakkad, additional, Liu, Zengping, additional, Yang, Binxia, additional, Bhargava, Mayuri, additional, Wong, Daniel Soo Lin, additional, Tan, Queenie Shu Woon, additional, Wong, Claudine See Wei, additional, Wang, Hao Fei, additional, Al-Mubaarak, Abdurrahmaan, additional, Chou, Chai, additional, Cheung, Chui Ming Gemmy, additional, Lim, Kah Leong, additional, Barathi, Veluchamy Amutha, additional, Hunziker, Walter, additional, Lingam, Gopal, additional, Hu, Tim Xiaoming, additional, and Su, Xinyi, additional

Published
2023
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29. Electrohydrodynamic Jet-Printed Ultrathin Polycaprolactone Scaffolds Mimicking Bruch’s Membrane for Retinal Pigment Epithelial Tissue Engineering

Author

Liu, Hang, primary, Wu, Fan, additional, Chen, Renwei, additional, Chen, Yanan, additional, Yao, Kai, additional, Liu, Zengping, additional, Harshad Parikh, Bhav, additional, Jing, Linzhi, additional, Liu, Tiange, additional, Su, Xinyi, additional, Sun, Jie, additional, and Huang, Dejian, additional

Published
2022
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31. Antiangiogenic Nanomicelles for the Topical Delivery of Aflibercept to Treat Retinal Neovascular Disease

Author

Zhao, Xinxin, primary, Seah, Ivan, additional, Xue, Kun, additional, Wong, Wendy, additional, Tan, Queenie Shu Woon, additional, Ma, Xiaoxiao, additional, Lin, Qianyu, additional, Lim, Jason Y. C., additional, Liu, Zengping, additional, Parikh, Bhav Harshad, additional, Mehta, Karishma N., additional, Lai, Joel Weijia, additional, Yang, Binxia, additional, Tran, Kim Chi, additional, Barathi, Veluchamy Amutha, additional, Cheong, Kang Hao, additional, Hunziker, Walter, additional, Su, Xinyi, additional, and Loh, Xian Jun, additional

Published
2021
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33. Additional file 1 of cGMP-grade human iPSC-derived retinal photoreceptor precursor cells rescue cone photoreceptor damage in non-human primates

Author

Lingam, Swathi, Liu, Zengping, Yang, Binxia, Wong, Wendy, Parikh, Bhav Harshad, Ong, Jun Yi, Goh, Debbie, Wong, Daniel Soo Lin, Tan, Queenie Shu Woon, Tan, Gavin S. W., Holder, Graham E., Regha, Kakkad, Barathi, Veluchamy Amutha, Hunziker, Walter, Lingam, Gopal, Zeng, Xianmin, and Su, Xinyi

Subjects
sense organs
Abstract

Additional file 1: Table S1: List of primary antibodies used in the study. figure S1: Estimation of GFP expression by FACS. After lentivirus infection, 95% of the RPC cells expressed GFP (left panel). There was no GFP in the non-infection control (right panel). figure S2: Controls for GFP, SC121 and AMA staining. (A-D) Secondary antibody only controls for Alexa 488 (B), Alexa 568 (C) and Alexa 647 (D). The background for all secondary antibodies appears to be high. (E-H) GFP, SC121 and anti-human mitochondrial antibody staining in non-operated NHP retina. None of the antibodies specifically stained anything in the non-operated control retina. The brightness/ contrast was artificially increased to identify any weakly stained cells. Hoechst 33342 was used to stain the INL and ONL layers (A, E). Scale bar 10 µm. figure S3: Cross reactivity of human lamin B2 (hLMNB2) antibody with the NHP retina. Tissue sections collected from NHP eyes post-photoreceptor precursor transplantation were stained with the human marker SC121 (B) and the human specific nuclear marker lamin B2 (C). The human nuclear marker antibody showed high cross-reactivity with the NHP tissue (C, D). Nuclei were stained with Hoechst 33,342 (H33342). Scale bar: 10 µm.

Published
2021
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34. Additional file 1 of Submacular integration of hESC-RPE monolayer xenografts in a surgical non-human primate model

Author

Liu, Zengping, Ilmarinen, Tanja, Tan, Gavin S. W., Hongisto, Heidi, Wong, Edmund Y. M., Tsai, Andrew S. H., Al-Nawaiseh, Sami, Holder, Graham E., Su, Xinyi, Barathi, Veluchamy Amutha, Skottman, Heli, and Stanzel, Boris V.

Subjects
sense organs
Abstract

Additional file 1: Figure S1. In vitro characterization of the hESC-RPE cells. Figure S2. Graphical representation of shipment temperatures and times. Figure S3. Postoperative in vivo analysis with multimodal imaging in an animal with unfavorable outcome. Figure S4. Effect of different subretinal BSS injection modes on fluorescein angiography. Figure S5. Retinal gliosis after using Ca2+ and Mg2+-free BSS as the tamponade agent during surgery. Table S1. Summary of details of antibodies and conditions. Table S2. Summary of details of in vivo ophthalmic follow up of transplanted ESC-RPE grafts in NHPs.

Published
2021
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36. Submacular Integration of hESC-RPE Monolayer Xenografts in a Surgical Non-Human Primate Model

Author

Liu, Zengping, primary, Ilmarinen, Tanja, additional, Tan, Gavin S.W, additional, Hongisto, Heidi, additional, Wong, Edmund Y.M, additional, Tsai, Andrew S.H, additional, Al-Nawaiseh, Sami, additional, Holder, Graham E, additional, Su, Xinyi, additional, Barathi, Veluchamy Amutha, additional, Skottman, Heli, additional, and Stanzel, Boris V., additional

Published
2021
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42. Adult-Human Retinal Pigment Epithelial Cell Transplantation Supports Retinal Function in Non-Human Primate Models

Author

Liu, Zengping, primary, Parikh, Bhav Harshad, additional, Tan, Queenie Shu Woon, additional, Wong, Daniel Soo Lin, additional, Ong, Kok Haur, additional, Yu, Weimiao, additional, Seah, Ivan, additional, Holder, Graham E., additional, Hunziker, Walter, additional, Tan, Gavin Siew Wei, additional, Barathi, Veluchamy Amutha, additional, Lingam, Gopal, additional, Stanzel, Boris V., additional, Blenkinsop, Timothy A., additional, and Su, Xinyi, additional

Published
2020
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43. Sustained delivery of anti-VEGFs from thermogel depots inhibits angiogenesis without the need for multiple injections

Author

Xue, Kun, primary, Zhao, Xinxin, additional, Zhang, Zhongxing, additional, Qiu, Beiying, additional, Tan, Queenie Shu Woon, additional, Ong, Kok Haur, additional, Liu, Zengping, additional, Parikh, Bhav Harshad, additional, Barathi, Veluchamy Amutha, additional, Yu, Weimiao, additional, Wang, Xiaomeng, additional, Lingam, Gopal, additional, Hunziker, Walter, additional, Su, Xinyi, additional, and Loh, Xian Jun, additional

Published
2019
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45. A Step by Step Protocol for Subretinal Surgery in Rabbits

Author

Al-Nawaiseh, Sami, primary, Thieltges, Fabian, primary, Liu, Zengping, primary, Strack, Claudine, primary, Brinken, Ralf, primary, Braun, Norbert, primary, Wolschendorf, Marc, primary, Maminishkis, Arvydas, primary, Eter, Nicole, primary, and Stanzel, Boris V., primary

Published
2016
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47. A new highly transparent injectable PHA-based thermogelling vitreous substituteElectronic supplementary information (ESI) available. See DOI: 10.1039/c9bm01603a

Author

XueThese authors contributed equally to this work., Kun, Liu, Zengping, Jiang, Lu, Kai, Dan, Li, Zibiao, Su, Xinyi, and Loh, Xian Jun

Abstract

Transparency is an important criterion for the application of biomaterials to the eye and essential for use as a vitreous substitute. However, there is a lack of understanding on the features of transparent hydrogels and the boundaries of transparency of various hydrogel systems. In this paper, we tune the poly[(R)-3-hydroxybutyrate-(R)-3-hydroxyhexanoate] (PHBHx) content of biodegradable PHBHx-based polyurethane thermogels and show that the amount of hydrophobic PHBHx correlates with hydrogel cloudiness. We found that PHxEP-0.5 hydrogel shows high light transmittance and suitable gel properties as a vitreous substitute. The PHxEP-0.5 hydrogel is able to maintain transparency when implanted in rabbit eyes as opposed to a cloudy gel and shows negligible inflammation and preservation of the retinal structure over 6 months. In conclusion, we show that PHBHx-based thermogels can be tuned for transparency and are biocompatible in the rabbit eye. These results could be instructive for the design of a new generation of injectable transparent vitreous substitutes.

Published
2020
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48. Lutein Modulates Cellular Functionalities and Regulates NLRP3 Inflammasome in a H2O2-Challenged Three-Dimensional Retinal Pigment Epithelium Model

Author

Liu, Hang, Wu, Chushan, Hu, Shiyin, Leng, Bin, Lou, Xiaowei, Liu, Zengping, Su, Xinyi, and Huang, Dejian

Abstract

Excessive hydrogen peroxide (H2O2) generated during retinal cell metabolic activity could lead to oxidative degeneration of retinal pigment epithelium (RPE) tissue, a specific pathological process implicated in various retinal diseases resulting in blindness, which can be mitigated by taking dietary antioxidants to prevent inflammation and impaired cellular dysfunction. This study tested the hypothesis that damages induced by oxidative stresses can be mitigated by lutein in a H2O2-challenged model, which was based on an ARPE-19 cell monolayer cultured on three-dimensional (3D)-printed fibrous scaffolds. Pretreating these models with lutein (0.5 μM) for 24 h can significantly lower the oxidative stress and maintain phagocytosis and barrier function. Moreover, lutein can modulate the NLRP3 inflammasome, leading to a ∼40% decrease in the pro-inflammatory cytokine (IL-1β and IL-18) levels. Collectively, this study suggests that the 3D RPE model is an effective tool to examine the capability of lutein to modulate cellular functionalities and regulate NLRP3 inflammation.

Published
2024
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50. Sustained delivery of anti-VEGFs from thermogel depots inhibits angiogenesis without the need for multiple injectionsElectronic supplementary information (ESI) available. See DOI: 10.1039/c9bm01049a

Author

XueThese authors contributed equally to this work., Kun, Zhao, Xinxin, Zhang, Zhongxing, Qiu, Beiying, Tan, Queenie Shu Woon, Ong, Kok Haur, Liu, Zengping, Parikh, Bhav Harshad, Barathi, Veluchamy Amutha, Yu, Weimiao, Wang, Xiaomeng, Lingam, Gopal, Hunziker, Walter, Su, Xinyi, and Loh, Xian Jun

Abstract

Anti-vascular endothelial growth factor (anti-VEGF) proteins are the gold-standard treatment for posterior eye segment proliferative vascular diseases such as Age-Related Macular Degeneration (AMD) and Diabetic Retinopathy (DR). However, the standard of care requires inconvenient monthly intravitreal injections. This underlies an unmet clinical need to develop alternative solutions for sustained delivery of biologics. In this paper, we demonstrated that anti-VEGFs can be encapsulated by a simple mild process into our polyurethane thermogel depots. By changing the hydrophilic–hydrophobic balance in the copolymer, anti-VEGF release rates can be modulated. The antibody in the thermogel partitions into protein domains which vary in size corresponding to the hydrophilicity balance of the polymer. Anti-VEGFs can be released in a relatively linear manner from the thermogel for up to 40 days in vitro. The encapsulated anti-VEGFs demonstrate anti-angiogenic bioactivity by inhibiting vessel outgrowth in rat ex vivochoroidal explants, and reducing vascular leakage in a VEGF-driven neovascularization rabbit model. In conclusion, we show that these thermogels can be tuned in terms of hydrophilicity and used for sustained delivery of bioactive anti-VEGFs. Physically cross-linked polyurethane thermoresponsive hydrogels could be a promising platform for sustained delivery of biologically active therapeutic proteins.

Published
2019
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