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Glutathione (GSH) plays an important role in human health. It is recommended to use dietary GSH to maintain GSH balance. In this study, a Hg.sup.2+-stabilized double-stranded probe for visual detection of GSH was developed. In the presence of GSH, Hg.sup.2+ in the probe reacted with GSH, and G-quadruplex sequence was released, followed by combining with K.sup.+ and hemin to generate G-quadruplex/hemin DNAzyme. DNAzyme oxidized 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) into the green product to indicate the presence of GSH. The probe had good selectivity, and the whole detection time was as low as 55 min. The sensitivity test indicated that the detection limit by the naked eye was 100 nM, and there was a good linear relationship between absorbance and GSH concentration with the linearity range of 0.05 to 2.5 [micro]M and LOD of 7.4 nM. Besides, this double-stranded probe was cheap and reliable in the analysis of real food samples with recoveries between 88.0-90.0% and RSD values of 1.6-2.2%. We believe that this probe has great potential for GSH detection in foods., Author(s): Qiqi Ning [sup.1] , Qiming Chen [sup.1] , Yuhao Huang [sup.1] , Yilu Wang [sup.1] , Yingying Wang [sup.1] , Zhanmin Liu [sup.1] Author Affiliations: (1) grid.39436.3b, 0000 0001 [...]

Medicinal food raw materials refer to those raw materials with food and possess medicinal value. They were particularly suitable for developing novel functional food. In this study, a novel functional food based on ultrafine grinding treatment of Ganoderma, yam, wolfberry, Rhizoma polygonati, raspberry, and euryale ferox (GYWRRE) with a specifically designed formula was developed and characterized. The results were shown that the ultrafine GYWRRE powder presented a relatively regular structure and smooth surface. It had notable decreases in fluidity and oil‐holding capacity but increases in characteristic nutrient content and antioxidant capacity. The ultrafine GYWRRE powder also could prevent oxidative stress in C2C12 cells by reducing the contents of reactive oxygen species and methylene diphenyl diisocyanate, inhibiting cell apoptosis, and increasing expressions of heme oxygenase‐1 and NQO1. The results of this study were demonstrated that medicinal food raw materials and ultrafine grinding had great applications potentials in developing functional food. Practical applications: Medicinal food refers to those natural ingredients that serve both as food and possess medicinal value. Ultrafine grinding technology is a meticulously engineered method capable of grinding raw materials to the micron level, thereby increasing their surface area, improving their bioavailability, and potentially enhancing their medicinal properties and bioavailability. This study developed an ultrafine grinding treatment of Ganoderma, yam, wolfberry, Rhizoma polygonati, raspberry and euryale ferox (GYWRRE) with a specifically designed formula. The characteristics of the ultrafine GYWRRE powder contained particle size, morphology, colorimetry, liquidity, hydration, adsorption, and characteristic nutrients were characterized. The antioxidant capacity in vitro and in cells as well as the possible mechanism were also explored. The results of this study will be helpful for development of novel functional foods. [ABSTRACT FROM AUTHOR]

In order to solve the problem that large items are not convenient to be carried under the condition without elevator,an electric auxiliary stair climbing machine based on the slider-crank mechanism is designed,in which 24 V lithium battery is used as the power supply and a decelerating motor is used as power output mechanism of the stair climbing machine. The optimization of slider guide inclination is analyzed by using motion of UG software. Through the experiment of the physical prototype,the distance difference between the slider movement between the highest point and the lowest point is 17. 674 cm,which meets the coordination standard of the building staircase module of our country. The analysis of ANSYS shows that the strength meets the stress requirements during the movement of the stair climbing machine,the stair climbing machine can run smoothly on the stairs,the safety and reliability of the stair climbing process will be ensured through the mechanical structure.

Glutathione (GSH) plays an important role in human health. It is recommended to use dietary GSH to maintain GSH balance. In this study, a Hg2+-stabilized double-stranded probe for visual detection of GSH was developed. In the presence of GSH, Hg2+ in the probe reacted with GSH, and G-quadruplex sequence was released, followed by combining with K+ and hemin to generate G-quadruplex/hemin DNAzyme. DNAzyme oxidized 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) into the green product to indicate the presence of GSH. The probe had good selectivity, and the whole detection time was as low as 55 min. The sensitivity test indicated that the detection limit by the naked eye was 100 nM, and there was a good linear relationship between absorbance and GSH concentration with the linearity range of 0.05 to 2.5 µM and LOD of 7.4 nM. Besides, this double-stranded probe was cheap and reliable in the analysis of real food samples with recoveries between 88.0–90.0% and RSD values of 1.6–2.2%. We believe that this probe has great potential for GSH detection in foods. [ABSTRACT FROM AUTHOR]

Shanghai has become an international shipping center in the world. In this study, the multiyear measurements and the high resolution air quality model with hourly ship emission inventory were combined to determine the influence of ship emissions on urban Shanghai. The aerosol time-of-flight mass spectrometer (ATOFMS) measurements were carried out at an urban site from April 2009 to January 2013. During the entire sampling time, most of the half-hourly averaged number fractions of primary ship emitted particles varied between 1.0-10.0%. However, the number fraction could reach up to 50% during the ship plume cases. Ship-plume-influenced periods usually occurred in spring and summer. The simulation of Weather Research and Forecasting/Community Multiscale Air Quality model (WRF/CMAQ) with hourly ship emission inventory provided the highly time-resolved concentrations of ship-related air pollutants during a ship plume case. It showed ships could contribute 20-30% (2-7 ?g/m3) of the total PM2.5 within tens of kilometers of coastal and riverside Shanghai during ship-plume-influenced periods. Our results showed that ship emissions have substantial contribution to the air pollution in urban Shanghai. The control measures of ship emission should be taken considering its negative environment and human health effects. [ABSTRACT FROM AUTHOR]