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MCF-7, DU-145, EC-109 and A549 tumor cells (5 x 10(4)nml) at mid-exponential phase were treated for 24h with different concentrations of Toxoplasma gondii tachyzoites (1 x 10(4)/ml-32x10(4)/ml). The measurement of tumor cell growth inhibition rate was performed by using CCK-8 kit. The tachyzoites (1 x 10(4)/ml-4 x 10(4)/ml) were co-cultured with A549 tumor cells for 24 h. Flow cytometry techniques was applied to investigate the expression of p53 and Bcl-2. The results showed a remarkable inhibitory effect of the parasites on tumor cell proliferation, which presented a dosage dependence. MCF-7, DU-145, EC-109 and A549 cell lines treated with 1 x 10(4) ml T. gondii tachyzoites presented an inhibition rate of 19.7%, 4.5%, 28.0%, and 20.8%, respectively. There was a significant difference between experimental groups and the control (P < 0.05). T. gondii induced cell apoptosis in A549 tumor cells, p53 and Bcl-2 gene expression was respectively up-regulated and down-regulated.

Objective: To establish better treatment for Monteggia fracture by evaluating the operative effect and function rehabilitation in children., Methods: From 1994 to 2001, 78 children with Monteggia fracture (30 cases of new fracture, 48 cases of old fracture) were treated with open reduction and internal fixation. The patients were randomly divided into two groups. In the first group (45 cases, 16 new and 29 old), radius-humeral joint was fixed with a Kirschner wire after reduction and without fixation of ulna fracture; in the second group(33 cases, 14 new and 19 old), both radiohumeral joint and ulna fracture were fixed with Kirschner wire. Two groups were treated with plaster-splint after operation. The effect of operation was evaluated according to the function criteria for bending elbow and rotation of forearm., Results: All patients were followed up 6 months to 7 years (4.6 years on average). All wound healed well without bone nonunion, delayed union and infection after operation. In the first group, 37 cases were rated as excellent, 5 good and 3 poor. The effective rate was 93.3%. In the second group, 22 cases were rated as excellent, 7 good and 4 poor. The effective rate was 87.9%. There was no significant difference between two groups (P > 0.05)., Conclusion: Surgical treatment is the choice for Monteggia fracture in children. It should be treated with single Kirschner wire fixing after open reduction of radiohumeral and plaster-splint. This method is simple, safe and has satisfactory results in fracture healing and function rehabilitation after operation.