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AIM: To investigate the effect of inhibiting Ca2+/calmodulin-dependent protein kinase Ⅱ(CAMKⅡ)expression in adult retinal pigment epithelial cell line-19(ARPE-19)cells on the migration, invasion, and tube formation of human umbilical vein endothelial cells(HUVECs)in a non-contact co-culture system.METHODS: RNA sequencing was performed on ARPE-19 cells overexpressing CAMKⅡ-δ, and bioinformatics was used to analyze the biological functions of the differentially expressed genes. Transwell inserts was used to construct a non-contact co-culture system of ARPE-19 and HUVECs. The experimental groups included: blank group: only HUVECs were inoculated without ARPE-19 cells; control group: ARPE-19 and HUVECs cells were co-cultured with complete medium; AIP group(CAMKⅡ inhibition group): ARPE-19 cells in AIP(160 nmol/L)were co-cultured with HUVECs in complete medium. The migration, invasion and tube formation abilities of HUVECs were detected. The protein expression levels of CAMKⅡ/AMPK/mTOR/VEGFA were detected by Western blotting.RESULTS:Bioinformatics analysis found that the differentially expressed genes could affect biological processes such as cell growth and death and cell movement. The scratch test and transwell migration test showed that the relative mobility of HUVECs in the AIP group was significantly lower than that in the control group(all P0.05). Western blotting results showed that the expression levels of CAMKⅡ, P-mTOR, and VEGFA proteins in the AIP group were significantly lower than those in the control group, while the expression level of the P-AMPK protein was significantly higher than that in the control group(all P

A series of filter rods (FRs) with sodium alginate (SA) modification were prepared by a simple one-pot pre-treatment surface modification method for the retention of typical smoke components. The influences of SA on the physico-chemical properties and adsorption behaviors (or retention characteristics) toward FRs were investigated in detail. Based on the results of XRD, BET, and SEM, it can be inferred that SA modification has no influences on compositions and textural properties of FRs. However, surface modification of FR by SA can greatly improve the hydrophilicity of FR, which is crucial for water retention. In situ diffuse Fourier-transform infrared spectroscopy results demonstrate that FR modified by 2 g·L−1 SA presents superior adsorption character for acetone. The differences in the adsorption or retention of water and acetone are ascribed to surface enriched functional groups tuned by surface engineering modification. By contrast, the modification of SA had no obvious effect on the adsorption of CO at room temperature. By contrast, at high temperature, low modification concentration is conducive to the CO2 and CO adsorption. It presents a good perspective for the rational design of new filter materials to reduce the release of harmful components in cigarette smoke.

Background: Fibroblast-derived exosomes can regulate the electrical remodeling of cardiomyocytes, and the intermediate-conductance calcium-activated potassium channel (KCa3.1) is important in atrial electrical remodeling. However, the underlying molecular mechanisms remain unclear. This study aimed to investigate the regulation of cardiac electrophysiology by exosomes linked to KCa3.1. Methods: Atrial myocytes (AMs) and atrial fibroblasts were isolated from Sprague-Dawley suckling rats and cultured individually. The cellular atrial fibrillation (AF) model was established via electrical stimulation (1.0 v/cm, 10 Hz), and fibroblast-derived exosomes were isolated via ultracentrifugation. Exosomes were co-cultured with AMs to investigate their influences on KCa3.1 and the underlying mechanisms. Nanoparticle tracking analysis and transmission electron microscopy were used to measure exosome particle sizes and concentrations. Whole-cell patch clamp was applied to record the current density of KCa3.1 and action potential duration (APD). The expression of miR-21-5p was detected by reverse-transcription polymerase chain reaction (RT-PCR). Western blotting or immunofluorescence was used to measure the expression of exosomal markers, Akt phosphorylation, and KCa3.1. Results: Rapid pacing promoted the secretion of exosomes from atrial fibroblasts and miR-21-5p expression in atrial fibroblasts and exosomes. KCa3.1 protein expression and current density significantly increased, and APD50 and APD90 were sharply shortened after rapid pacing in AMs. TRAM-34 (KCa3.1 blocker) extended APD and reduced susceptibility to AF. KCa3.1 and P-AKT expressions were amplified after co-culturing AMs with exosomes secreted by atrial fibroblasts. In contrast, the increase in KCa3.1 expression was reversed after the cells were co-cultured with exosomes secreted by atrial fibroblasts that were transfected with miR-21-5p inhibitors or after the use of LY294002, a PI3K/Akt pathway inhibitor. Conclusions: Rapid pacing promoted the secretion of exosomes from fibroblasts, and miR-21-5p was upregulated in exosomes. Moreover, the miR-21-5p-enriched exosomes upregulated KCa3.1 expression in AMs via the PI3K/Akt pathway.

The rapid development of perioperative intensive care in cardiac surgery has greatly improved the prognosis for patients undergoing major cardiovascular surgery. In the field of hemodynamics, besides traditional methods, such as a variety of arterial catheters and central venous catheter, thermodilution and echocardiography have gradually becoming routines in hemodynamic monitoring, but their clinical values require further investigation. At the same time, optimizing micro-circulation, in order to meet the demand of oxygen and metabolism, is the goal of hemodynamic resuscitation. The management of V-A extracorporeal membrane oxygenation (ECMO) in the perioperative period of cardiac surgery depends on persistent hemodynamic monitoring. Volume management, vasoactive drugs, inotropes and vasodilators are still keys in pharmacological management. In addition, many mechanical circulatory assist devices have brought more treatments and hope for end-stage heart failure and severe cardiopulmonary dysfunction.

This paper summarized the nursing management of constipation in a stroke patient. Based on the brain-gut axis theory, the moxibustion combined with auricular acupoint pressing therapy and individualized nursing interventions were carried out to relieve the abdominal distension and anxiety of the patient. Based on the interaction of the brain-gut axis theory, nursing by syndrome differentiation is conductive to relieve the constipation and improve the recovery of gastrointestinal function in patients with post-stroke constipation. (本文回顾1例脑卒中后便秘患者的临床资料, 总结护理经验。基于脑-肠轴理论实施艾灸及耳穴压豆治疗, 同时配合健康宣教等个体化护理措施, 患者便秘伴随症状腹胀、焦虑都得到改善。基于脑-肠轴理论大脑与胃肠相互影响、相互调节思想对患者进行辨证施护, 解决患者便秘及焦虑症状, 有利于患者胃肠功能的恢复。)

This study aimed to explore the characteristics of bongkrekic acid (BA) produced by Burkholderia gladioli pathovar cocovenenans (BGC) and the effects of ambient temperature and humidity on BA production in the fermented glutinous rice flour (FGRF). In the specific environment with various ambient temperatures (10±1, 15±1, 20±1, 25±1, 30±1, 36±1 ℃) and relative humidity (20%, 40%, 60%), The FGRF was incubated with different bacteria counts of BGC (103, 105, 107 CFU/mL), and BA in the FGRF was measured with high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). The results revealed that BA could be detectable after only one day of incubation. The BA produced and accumulated by BGC with an initial bacteria count of 103 CFU/mL was more than that in the groups with the initial bacteria counts of 105 and 107 CFU/mL (with a difference of 1-3 μg/kg between groups). Lower temperatures (10±1, 15±1, 20±1 ℃) and humidity (20%) were found to reduce the BA production of BGC in the FGRF. To minimize the risk of foodborne BA poisoning, it is recommended to consume freshly prepared FGRF promptly and avoid storing it for extended periods under hot and humid conditions.

The accumulation and incineration of waste tires have caused great damage to the environment. Therefore, recycling waste tires efficiently and economically has become an important issue in protecting world resources and the environment. In this study, 5%, 10%, and 15% rubber particles were used to replace fine sand in concrete, and basalt fibers (0.1%, 0.2%, and 0.3%) and polypropylene fibers (0.1%, 0.15%, and 0.2%) were added to rubber concrete to prepare hybrid fiber rubber concrete (HFRC). The experiments investigated the changes of mechanical properties of basalt (BF), polypropylene (PPF) fibers, and rubber particles when they were blended into concrete respectively, and the degree of influence of each factor on the mechanical and carbonation resistance of HFRC was investigated based on the orthogonal test. The results show that the rubber concrete’s mechanical and carbonation properties are improved to varying degrees after adding hybrid fibers. When the basalt fiber content is 0.2% and the polypropylene fiber content is 0.15%, the overall mechanical properties of HFRC reach the best state. When the basalt fiber content is 0.2%, the polypropylene fiber content is 0.15%, and the rubber content is 5%, the carbonation resistance of HFRC is the most ideal. In addition, the microstructure of HFRC was observed by scanning electron microscope.

Objective: To compare the performance of cryogenic refrigeration system using LNG wasted cold and ammonia cooling. Methods: The thermodynamic and economic models of the two systems were established by MATLAB. Under the basic parameters, the working medium concentration suitable for LNG low-temperature refrigeration system was selected, and the cold storage temperature and refrigeration capacity were changed respectively. The thermal economy of the two systems was compared and analyzed. Results: With the increase of ethylene glycol concentration, LNG mass flow, system power consumption and cold storage capacity increased, COP decreased, but ethylene glycol mass flow remained unchanged; With the increase of cold storage temperature, the system COP increased, while the working medium mass flow and power consumption decrease. Similarly, with the increase of refrigeration capacity, the mass flow and power consumption increase, while the COP of ammonia low-temperature refrigeration system remained unchanged and the COP of LNG low-temperature refrigeration system increased. In the range of 5~20 kW, the COP of LNG low-temperature refrigeration system was lower than that of ammonia low-temperature refrigeration system, and the opposite was true in the range of 20~30 kW. The comparison of economic performance showed that the investment cost of LNG cryogenic refrigeration system was much lower than that of ammonia cryogenic refrigeration system. Conclusion: Under the same working conditions, the thermodynamic and economic performance of the low-temperature refrigeration system using LNG wasted cold is better than that of the traditional ammonia low-temperature refrigeration system.

BackgroundThe muscle-related indicator is removed from Global Leadership Initiative on Malnutrition (GLIM) criteria implemented in China for many reasons. Patients with hematopoietic stem cell transplants are at nutrition risk and can enter into the second step of GLIM; thus, they are suitable for learning the diagnosing malnutrition significance between primary GLIM and GLIM-China criteria. This article aims to explore the role of muscle mass in the diagnostic criteria of malnutrition and the effects of GLIM-China for diagnosing malnutrition.MethodsA total of 98 inpatients with hematopoietic stem cell transplants (HSCT) were recruited. Nutrition risk was assessed by using the Nutritional Risk Screening 2002 (NRS-2002). Appendicular skeletal muscle mass (ASMI) and fat-free mass index (FFMI) were determined using the bioelectrical impedance analysis (BIA) method. Malnutrition is defined by GLIM-China, GLIM, and PG-SGA. We use erythrocyte sedimentation rate (ESR) and C-reactive protein (CRP) to assess inflammation in GLIM and GLIM-China. The correlation or consistency among ASMI, FFMI, ESR, CRP, GLIM-China, GLIM, and PG-SGA was evaluated, respectively.ResultsOne hundred percent instead of the patients had nutritional risk. The magnitude of malnutrition using PG-SGA, GLIM, and GLIM-China was 75.5, 80.6, and 64.3%, respectively. GLIM-China and PG-SGA showed the same performance (p = 0.052 vs. 1.00) and agreement (kappa = 0.404 vs. 0.433, p < 0.0001) with the FFMI. Consistency was noted between ASMI and PG-SGA in the assessment of malnutrition (p = 0.664) with a good agreement (kappa = 0.562, p = 0.084). ASMI and FFMI could determine muscle mass reduction, which could not be determined by BMI, albumin (ALB), and pre-albumin (pre-ALB); 34% of GLIM-China (–) patients were with low ASMI, and 40% with low FFMI; 30.0% of patients with PG-SGA (

ObjectiveTo construct a cell line that can stably express human phospholamban（PLN） and initially explore its application in the study of myocardial toxicity mechanism.MethodsFastCloning method was used to insert the open reading frame sequence of target gene PLN into eukaryotic expression vector pcDNA5/FRT/TO（hereinafter referred to as pDFT） to construct the pDFT-PLN-Flag plasmid. The Flp-InTM T-RExTM 293 cells were generated by cotransfection of the constructed plasmid and pOG44 plasmid to express the target gene. Successfully recombined monoclonal cell lines were screened by hygromycin B resistance. Western blot and indirect immunofluorescence （IFA） were used to examine the expression of the target protein in recombinant cells. After the cell line was exposed to aconitine, it was verified by Western blot to detect changes in PLN protein phosphorylation.ResultsAfter PCR amplification of the recombinant plasmid and DNA electrophoresis, the length of the amplified product is the same as the known PLN gene fragment, which is consistent with the open reading frame （ORF） sequence of the human PLN gene after sequencing. IFA and Western blot showed that the constructed proliferation cell line can stably express high levels of human PLN under induction and regulation. Preliminary results showed that the phosphorylation level of Thr17-PLN decreased after two hours of exposure to 1 μmol/L aconitine.ConclusionThis human cell line can stably express PLN and can be used to study the mechanism of action of aconitine on the cell at molecular level.

Logging data of Tazhong clastic rock formation shows that cementing quality of water layer is poor and the efficiency of cement displacement is low. Bohai Drilling Co., Ltd. has adopted a cement slurry system with excellent anti-channeling performance in Tazhong block and strictly controlled the cementing construction procedure, however, the cementing quality problem of water layer still exists. According to the cementing quality analysis, the influence of ions in formation water in Tazhong clastic strata on cement slurry performance was researched in this paper. The results show that ions in formation water has no negative impact on the loss of water and gas channeling resistance of the cement slurry system, however, has some adverse effects on the rheological property, thickening property and hardened strength. Furthermore, cement slurry consistency increasing caused by formation water is the key factor leading to poor cementing quality. Studies show that the carboxylic acid group and the magnesium ion chelate to form a network structure, which binds the free water in the cement slurry and causes the deterioration of the rheology. The magnesium resistance ability of the polymer becomes worse, when the proportion of the carboxylic acid group increases. Therefore, in order to improve rheology of cement slurry, a fluid loss agent with a small number of carboxylic acid group or no carboxylic acid group is required when the slurry or formation water containing a certain amount of magnesium ion. This investigation provides the theoretical guidance of the selection of cementing additives in the areas of which water containing high dosage of magnesium ion.

Ti3SiC2 ceramic bulk with a relative density greater than 99% was prepared by hot pressing sintering (HP) using Ti3SiC2 powder as raw material, and the hardness, flexural strength and fracture toughness of HP Ti3SiC2 bulk were 775HV, 520.46 MPa and 7.62 MPa·m1/2, respectively. To evaluate its oxidation-resistance and thermal shock resistance, ultra-high temperature ablation tests were conducted under oxyacetylene flame without cooling. The results show that the Ti3SiC2 ceramics remain flat within 10 s of the ablation, no macrocracks within 25 s.SEM and XRD analysis reveal that Ti3SiC2 samples are decomposed and oxidized during the ultra-high temperature ablation, elements Si and C are oxidized into gaseous Si-O and C-O compounds, TiO2 (rutile) with a loose structure remains on the surface of the sample. To analyze the internal oxide layer, a dense layer composed of rutile TiO2 and Al2TiO5 is observed, under which an Al2O3 particle-enriched layer is found covering the substrate. The dense internal oxide layer can effectively prevent O2 from diffusing inward, thereby reducing the oxidation rate of Ti3SiC2. The high melting point and high viscosity Al2O3 particle layer can absorb a large amount of heat and then reduce the transfer of heat flow to the Ti3SiC2 substrate, thus improving the anti-ablation of the Ti3SiC2 material.

Lidocaine can inhibit the malignant development of various human cancers. Circular RNA (circRNA) dynein 1 heavy chain gene (circ_DYNC1H1) acted as a pro-cancer molecule in hepatocellular carcinoma (HCC). This study aimed to explore whether the function of lidocaine was related to the oncogenic circ_DYNC1H1 in HCC. Colony formation assay and 3-(4,5-dimethylthiazol-2-y1)-2, 5-diphenyl tetrazolium bromide (MTT) assay were used for proliferation detection. Cell apoptosis was assessed by flow cytometry, and migration or invasion was determined by the transwell assay. The levels of circ_DYNC1H1, microRNA-520a-3p (miR-520a-3p), and ubiquitin-specific protease 14 (USP14) were examined using the quantitative reverse transcriptase polymerase chain reaction (qRT-PCR). Protein levels were measured using western blot. The binding between miR-520a-3p and circ_DYNC1H1 or USP14 was confirmed by the dual-luciferase reporter assay. In vivo assay was conducted by a xenograft model in mice. Lidocaine reduced proliferation, migration, and invasion but promoted apoptosis in HCC cells. The circ_DYNC1H1 expression was downregulated in lidocaine-treated HCC cells. The inhibitory effect of lidocaine on HCC progression was weakened after circ_DYNC1H1 overexpression. miR-520a-3p was a target of circ_DYNC1H1, and the function of lidocaine was related to the regulation of circ_DYNC1H1/miR-520a-3p axis. USP14 served as a target for miR-520a-3p, and circ_DYNC1H1 could sponge miR-520a-3p to regulate the USP14 expression. The lidocaine-induced suppression of HCC development was also achieved by mediating the miR-520a-3p/USP14 axis. In vivo assay revealed that lidocaine suppressed the tumor growth of HCC by reducing the expression of circ_DYNC1H1 to affect the levels of miR-520a-3p and USP14. Our results clarified that lidocaine impeded tumor progression via targeting the circ_DYNC1H1/miR-520a-3p/USP14 axis in HCC cells.

To develop nursing adverse events’ reporting content of hospital.

Objective To study the effect of down-regulation of long non-coding RNA (lncRNA) HOXA cluster antisense RNA 2 (HOXA-AS2)/miR-20a pathway on apoptosis and invasion of skin squamous cell carcinoma cells in vitro. Methods qRT-PCR was used to detect the expression of HOXA-AS2 and miR-20a in 50 clinical specimens of skin squamous cell carcinoma and paired adjacent tissues. Skin squamous carcinoma cell line A431 was transfected with HOXA-AS2 siRNA, and the changes in cell proliferation, clonogenic ability, apoptosis, migration and invasion were evaluated by using MTT assay, clone formation experiment, flow cytometry, and Transwell assay respectively. Western blotting was performed to detect the expression of MMP-2, MMP-9, and cleaved caspase-3 proteins in the cells. Bioinformatics software predicted that HOXA-AS2 and miR-20a had complementary binding sites, and a luciferase reporter system was employed to verify their targeting relationship. A431 cells were co-transfected with HOXA-AS2 siRNA and miR-20a inhibitor, and the changes in cell proliferation, clone formation, apoptosis, migration and invasion were assessed. Results The expression level of HOXA-AS2 was increased in skin squamous cell carcinoma tissues. Transfection of A431 cells with HOXA-AS2 siRNA significantly suppressed the cell proliferation, clone formation, invasion and migration, promoted cell apoptosis, decreased the expression of MMP-2 and MMP-9 proteins, and increased the expression of cleaved caspase-3 protein. Down-regulation of HOXA-AS2 significantly up-regulated miR-20a expression. The expression level of miR-20a in skin squamous cell carcinoma tissues was negatively correlated with that of HOXA-AS2. In A431 cells, miR-20a inhibitor obviously reversed the effects of HOXA-AS2 siRNA on proliferation, clone formation, invasion, migration and apoptosis of the cells. Conclusion Down-regulation of HOXA-AS2/miR-20a pathway can promote the expression of miR-20a, suppress the proliferation, clone formation, invasion and migration, and induce apoptosis of skin squamous cell carcinoma cells in vitro.

Abstract Background There are several types of tenodesis reconstruction designed for subtalar instability. However, no comprehensive comparison has been conducted among these procedures in terms of their correcting power so far. The objective of this study is to evaluate the biomechanical behaviors of 5 representative procedures through finite element analysis. Methods Finite element models were established and validated based on one of our previous studies. The Pisani interosseous talocalcaneal ligament (ITCL) reconstruction, Schon cervical ligament (CL) reconstruction and Choisne calcaneofibular ligament (CFL) reconstruction were compared on the model with the CFL, ITCL and CL sectioned. The Schon triligamentous reconstruction and Mann triligamentous reconstruction were compared on the model with the CFL, ITCL and CL, as well as the ATFL sectioned. The inversion and external/internal rotation were quantified at different ankle positions based on the rotational moment. Then, the stress in ligaments and reconstructed grafts and the contact characteristics of the subtalar joint under inversional stress test were calculated and compared accordingly. Results For single ligament reconstruction, the Choisne CFL reconstruction provided the greatest degree of correction for subtalar instability, followed by the Schon CL reconstruction and then the Pisani ITCL reconstruction. For triligamentous reconstruction, the Mann procedure outperformed the Schon procedure in alleviating the subtalar instability. Conclusion The finite element analysis showed that the Choisne CFL reconstruction and Mann triligamentous reconstruction provided the greatest degree of immediate postoperative subtalar stability. However, both procedures could not restore the biomechanical behaviors of the subtalar joint to normal. The long-term efficacy of these procedures warrants further investigation using a substantially larger sample of clinical cases.

To promote the development of nursing spiritual care and humanistic care, this article introduces the current status of the definition, assessment tools, and practice of spiritual care at home and abroad.

Three-dimensional angle-interlock woven composites (3DAWCs) are widely used for their excellent mechanical properties. The most significant feature is the existence of the undulated warp yarns along the thickness direction, which makes it interesting to study the mechanical properties in the warp direction. The quasi-static tensile behavior of a layer-to-layer 3DAWC along the undulated warp direction was studied by experimental and finite element analysis (FEA) methods. Based on the experimental results, the typical failure mode involving fibers, resin, and their interfaces was found. According to the FEA results, the stress concentration effect, key structural regions, and microstructural (yarn and resin) damage mechanism were obtained, which provided effective guidance for structural optimization design of the 3DAWC with stronger tensile resistance performance. In addition, the three-step progressive failure process of the 3DAWC under quasi-static tensile load was also described at the “yarn–resin” microstructural level.

The construction industry is among the pillars of China’s economic development. However, it causes high carbon emission and high energy consumption, which should be considered when drafting energy saving and emission reduction policies. The sustainable development of this industry lies in the effective estimation of carbon emission efficiency and implementation of energy-saving and emission reduction measures in accordance with local practical situations. First, investigations on the emission efficiency of the construction industry and relevant emission reduction policies in foreign developed countries were reviewed in this study. Second, the carbon emission efficiency of the construction industry in 13 prefecture-level cities in Hunan Province (China) from 2011 to 2017 were estimated using the SBM (Slack Based Measure) model involving unexpected outputs. Finally, specific suggestions on reducing the carbon emission of the construction industry were proposed. CO2 emissions in the construction industry increase annually as a response to economic development and urbanization. The carbon emission efficiency of Hunan Province’s construction industry maintained a stable growth rate in 2011-2017. The industry achieved an annual growth rate of 2.7% in 2017 from that in 2011. The carbon emission efficiency of Changsha City, Zhangjiajie City, and Yiyang City were relatively higher than those of other cities in the province. Such findings serve as a reference to the quantification of potential of Hunan Province in reducing the carbon emission, the formulation of specific carbon emission reduction goal, the augmentation of the means to evaluate energy saving and emission reduction, and the development of a low-carbon construction industry.

Final electromagnetic stirring (F-EMS) is widely used in the billet and bloom continuous casting process because it effectively improves the as-cast quality. Numerous industrial trials on F-EMS have been conducted; however, the real melt flow and heat transfer characteristics at the crater end remain unclear. In this study, based on a round billet special steel continuous casting process, a coupled three-dimensional numerical model was developed to describe the F-EMS phenomenon. The flow and solidification behavior of the melt in the F-EMS region were obtained by a segmentation calculation model, and the Darcy source term method was employed to suppress the velocity within the mushy region. The effect of stirring current intensity and frequency on the electromagnetic field, melt flow, and heat transfer was investigated numerically. The model was validated using the measured data of magnetic flux density in the stirrer center and the strand surface temperature. According to the simulation results, with every 100 A increase in the current intensity, the maximal magnetic flux density increases by 19.05 mT. The electromagnetic force significantly increases with the increase in current intensity. With the increase in current frequency within 20-40 Hz, the magnetic flux density decreases slightly, whereas the electromagnetic force increases. Moreover, a swirling flow field in the stirrer region is observed under the rotary electromagnetic force, and the tangential velocity of melt increases with the increase in current intensity and frequency. Additionally, the swirling flow enhances the local melt heat transfer at the radial direction of the round strand. As the current intensity and frequency increase, the temperature of the melt in the liquid core decreases, and the center solid fraction at the F-EMS-implemented position increases accordingly.

Multimodal analgesic strategy is pivotal for enhanced recovery after surgery. The objective of this trial was to assess the effect of subanesthetic esketamine vs. placebo combined with erector spinae plane block (ESPB) vs. intercostal nerve block (ICNB) on postoperative recovery following thoracoscopic lung resection. This randomized, controlled, 2×2 factorial trial was conducted at a university hospital in Suzhou, China. One hundred adult patients undergoing thoracoscopic lung surgery were randomized to one of four groups (esketamine-ESPB, esketamine-ICNB, placebo-ESPB, and placebo-ICNB) to receive i.v. esketamine 0.3 mg/kg or normal saline placebo combined with ESPB or ICNB using 0.375% ropivacaine 20 mL. All patients received flurbiprofen axetil and patient-controlled fentanyl. The primary outcome was quality of recovery (QoR) at 24 h postoperatively, assessed using the QoR-15 scale, with a minimal clinically important difference of 6.0. The median age was 57 years and 52% were female. No significant interaction effect was found between esketamine and regional blocks on QoR (P=0.215). The QoR-15 score at 24 h was 111.5±5.8 in the esketamine group vs. 105.4±4.5 in the placebo group (difference=6.1, 95% CI, 4.0-8.1; P

Abstract Background Hsp90-beta has been investigated to be correlated with the occurrence and development of tumor. The intention of this research was to test the level of Hsp90-beta in malignant pleural effusion (MPE) of patients with lung cancer and disclose the clinical significance of Hsp90-beta as a potential tumor marker for differential diagnosis of pleural effusion caused by lung cancer. Methods The level of Hsp90-beta was determined using enzyme-linked immunosorbent assay. Calculations of the Hsp90-beta threshold, the sensitivity and specificity for distinguishing MPE from benign pleural effusion were performed using receiver operator characteristic curve. Results The level of Hsp90-beta in MPE of lung cancer patients was higher than that in control individuals (P

For high-performance concrete (HPC) beams reinforced with hybrid Carbon Fiber Reinforced Polymer/Glass Fiber Reinforced Polymer (CFRP/GFRP) tendons, the nonlinear shell beam mixed element is studied and the whole mechanical process is analyzed. The CFRP/CFRP tendons are simulated with spatial beam element and the HPC beam is modeled with the layered shell element. With the coordination of nodal linear displacement and rotational displacement of CFRP/GFRP tendons element, the contribution of CFRP/GFRP element to stiffness matrix of nonlinear shell beam mixed element is deduced. Then, Jiang’s yielding criterion, Hinton’s crushing criterion, and so on, are used to describe the material nonlinearity of concrete. The new kind of nonlinear shell beam mixed element is achieved and the three-dimensional nonlinear calculation program is developed. The calculative results are consistent with the development trend of test results, which shows the correctness of the nonlinear shell beam mixed element and the reliability of the development program. The mixed element can accurately simulate the geometric configuration of CFRP tendons and realize the tension-compression-bending-shearing performance of CFRP tendons, which is helpful to fully reflect the reinforcement effect of reinforcement in the structure. The computational stiffness is defined and the stiffness degradation experiences three change processes. During the whole processes in the proposed typical load cases, the CFRP/GFRP tendons are still kept in the elastic stages.

A method to judge shale gas flow regimes based on digital core analysis is proposed in this work. Firstly, three-dimensional shale digital cores in an anonymous shale formation in the Sichuan Basin are reconstructed by a Markov Chain Monte Carlo (MCMC) algorithm based on two-dimensional Scanning Electron Microscope (SEM) images. Then a voxel-based method is proposed to calculate the characteristic length of the three-dimensional shale digital core. The Knudsen number for three-dimensional shale digital cores is calculated by the ratio of the molecular mean free path to the characteristic length and is used to judge the flow regimes under different reservoir conditions. The results indicate that shale gas flow regimes are mainly located at the slip flow and transition flow region. Furthermore, adsorption has no obvious influence on the free gas flow regimes. Because adsorption only exists in organic pores, three-dimensional inorganic pores and organic pores in the Haynesville shale formation are reconstructed by a MCMC algorithm based on two-dimensional SEM images. The characteristic lengths of the three-dimensional inorganic pores and three-dimensional organic pores are both calculated and gas flow regimes in organic pores and inorganic pores are judged.

Corn hulls were abundant and inexpensive byproducts of the corn dry or wet milling processes, but most of them were discarded as agro-wastes. The aim of this study was to extract the dietary fiber by hot-compressed water (HCW) from defatted corn hull and to determine the chemical properties. Results showed that temperature and time played critical roles in extraction efficiency; the maximal yield of dietary fiber A (DFA) extracted by HCW reached 33.0% at 150°C for 60 min. The yield of dietary fiber B (DFB) increased from 2.0% to 56.9% as the temperature increased from 110 to 180°C, while the yield of solid residue (SR) decreased from 88.7% to 27.7%. Fourier transform infrared spectroscopy (FT-IR) results demonstrated that C-H, O-H, C=O, COO- occurred in the DFA, SR and DFB. The dietary fiber polysaccharides consisted of arabinose, galactose, glucose, xylose and uronic acid.

The quasi-static tensile damage behavior of one type of layer-to-layer 3-Dimensional Angle-interlock Woven Composite (3DAWC) was tested and analyzed in this paper. Incorporated with the acoustic emission (AE) events monitoring, the mechanical behavior of the 3DAWC under tensile loading condition was characterized. The Load-Extension curve, Load/AE events-Time curves occurred during the entire testing process and tensile damage modes were recorded to characterize and summarize the mechanical properties and damage mechanism of the 3DAWC subjected to tensile loading. It was found that the tensile damage of the 3DAWC could be summarized into 3 steps. And each step has a distinct primary damage mode. Moreover, the resin cracks, resin-yarn interface debonding and yarn breakages were the main damage modes for the 3DAWC.

Based on the principle of human engineering,the eight-link mechanism with posture transformation of sitting and lying is designed. In order to ensure the stability of the mechanism and obtain better practical performance,the optimization design of the eight-link posture transformation mechanism is carried out.According to the process characteristic requirements,the specific constraint conditions and the optimization target of the eight-link posture transformation mechanism,the mathematical model of eight-link mechanism is constructed,the optimization design model of eight-link mechanism is established by using the ADAMS/View model,the optimization design of eight-link mechanism is carried out. From the results of the optimization design and the angle of the connecting rod,speed and acceleration curve before and after optimization,the optimization design coincides with the corresponding principle of human engineering, meeting the design requirements and purpose of the eight-link posture transformation mechanism.