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3 results on '"Liupei Huang"'

1. Isolation of Diverse Simian Arteriviruses Causing Hemorrhagic Disease

Author

Teressa M. Shaw, Samuel T. Dettle, Andres Mejia, Jennifer M. Hayes, Heather A. Simmons, Puja Basu, Jens H. Kuhn, Mitchell D. Ramuta, Cody J. Warren, Peter B. Jahrling, David H. O’Connor, Liupei Huang, Misbah Zaeem, Jiwon Seo, Igor I. Slukvin, Matthew E. Brown, and Adam L. Bailey

Subjects
arterivirid, Arteriviridae, arterivirus, engraft, induced pluripotent stem cell, iPSC, Medicine, Infectious and parasitic diseases, RC109-216
Abstract

Genetically diverse simian arteriviruses (simarteriviruses) naturally infect geographically and phylogenetically diverse monkeys, and cross-species transmission and emergence are of considerable concern. Characterization of most simarteriviruses beyond sequence analysis has not been possible because the viruses fail to propagate in the laboratory. We attempted to isolate 4 simarteriviruses, Kibale red colobus virus 1, Pebjah virus, simian hemorrhagic fever virus, and Southwest baboon virus 1, by inoculating an immortalized grivet cell line (known to replicate simian hemorrhagic fever virus), primary macaque cells, macrophages derived from macaque induced pluripotent stem cells, and mice engrafted with macaque CD34+-enriched hematopoietic stem cells. The combined effort resulted in successful virus isolation; however, no single approach was successful for all 4 simarteriviruses. We describe several approaches that might be used to isolate additional simarteriviruses for phenotypic characterization. Our results will expedite laboratory studies of simarteriviruses to elucidate virus-host interactions, assess zoonotic risk, and develop medical countermeasures.

Published
2024
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2. Isolation of Diverse Simian Arteriviruses Causing Hemorrhagic Disease.

Author

Shaw, Teressa M., Dettle, Samuel T., Mejia, Andres, Hayes, Jennifer M., Simmons, Heather A., Basu, Puja, Kuhn, Jens H., Ramuta, Mitchell D., Warren, Cody J., Jahrling, Peter B., O'Connor, David H., Liupei Huang, Zaeem, Misbah, Jiwon Seo, Slukvin, Igor I., Brown, Matthew E., and Bailey, Adam L.

Subjects
HEMORRHAGIC diseases, PLURIPOTENT stem cells, HEMATOPOIETIC stem cells, HEMORRHAGIC fever, VIRUS isolation
Abstract

Genetically diverse simian arteriviruses (simarteriviruses) naturally infect geographically and phylogenetically diverse monkeys, and cross-species transmission and emergence are of considerable concern. Characterization of most simarteriviruses beyond sequence analysis has not been possible because the viruses fail to propagate in the laboratory. We attempted to isolate 4 simarteriviruses, Kibale red colobus virus 1, Pebjah virus, simian hemorrhagic fever virus, and Southwest baboon virus 1, by inoculating an immortalized grivet cell line (known to replicate simian hemorrhagic fever virus), primary macaque cells, macrophages derived from macaque induced pluripotent stem cells, and mice engrafted with macaque CD34+- enriched hematopoietic stem cells. The combined effort resulted in successful virus isolation; however, no single approach was successful for all 4 simarteriviruses. We describe several approaches that might be used to isolate additional simarteriviruses for phenotypic characterization. Our results will expedite laboratory studies of simarteriviruses to elucidate virus-host interactions, assess zoonotic risk, and develop medical countermeasures. [ABSTRACT FROM AUTHOR]

Published
2024
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3. Robust engraftment of fetal nonhuman primate CD34-positive cells in immune-deficient mice

Author

Christopher J, Little, William J, Haynes, Liupei, Huang, Cross M, Daffada, Bryce K, Wolfe, Elizabeth, Perrin, John A, Simpson, Jenna A, Kropp Schmidt, Hayly M, Hinkle, Logan T, Keding, Ryan T, Behrens, David T, Evans, Dixon B, Kaufman, James A, Thomson, Thaddeus G, Golos, and Matthew E, Brown

Subjects
Immunology, Hematopoietic Stem Cell Transplantation, Granulocyte-Macrophage Colony-Stimulating Factor, Antigens, CD34, Mice, Transgenic, Mice, SCID, Cell Biology, Fetal Blood, Hematopoietic Stem Cells, Macaca mulatta, Mice, Animals, Humans, Immunology and Allergy
Abstract

Nonhuman primates (NHPs) represent one of the most important models for preclinical studies of novel biomedical interventions. In contrast with small animal models, however, widespread utilization of NHPs is restricted by cost, logistics, and availability. Therefore, we sought to develop a translational primatized mouse model, akin to a humanized mouse, to allow for high-throughput in vivo experimentation leveraged to inform large animal immunology-based studies. We found that adult rhesus macaque mobilized blood (AMb) CD34+-enriched hematopoietic stem and progenitor cells (HSPCs) engrafted at low but persistent levels in immune-deficient mice harboring transgenes for human (NHP cross-reactive) GM-CSF and IL3, but did not in mice with wild-type murine cytokines lacking NHP cross-reactivity. To enhance engraftment, fetal liver-derived HSPCs were selected as the infusion product based on an increased CD34hi fraction compared with AMb and bone marrow. Coupled with cotransplantation of rhesus fetal thymic fragments beneath the mouse kidney capsule, fetal liver-derived HSPC infusion in cytokine-transgenic mice yielded robust multilineage lymphohematopoietic engraftment. The emergent immune system recapitulated that of the fetal monkey, with similar relative frequencies of lymphocyte, granulocyte, and monocyte subsets within the thymic, secondary lymphoid, and peripheral compartments. Importantly, while exhibiting a predominantly naïve phenotype, in vitro functional assays demonstrated robust cellular activation in response to nonspecific and allogenic stimuli. This primatized mouse represents a viable and translatable model for the study of hematopoietic stem cell physiology, immune development, and functional immunology in NHPs. Summary Sentence : Engraftment of rhesus macaque hematopoietic tissues in immune-deficient mice yields a robust BLT/NeoThy-type primatized mouse model for studying nonhuman primate hematopoiesis and immune function in vivo.

Published
2022

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