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3. Therapeutic efficacy of artemether–lumefantrine and artesunate–amodiaquine for the treatment of uncomplicated Plasmodium falciparum malaria in Mali, 2015–2016

Author

Diarra, Youssouf, Koné, Oumar, Sangaré, Lansana, Doumbia, Lassina, Haidara, Dade Bouye Ben, Diallo, Mouctar, Maiga, Ababacar, Sango, Hamadoun A., Sidibé, Halidou, Mihigo, Jules, Nace, Douglas, Ljolje, Dragan, Talundzic, Eldin, Udhayakumar, Venkatachalam, Eckert, Erin, Woodfill, Celia J., Moriarty, Leah F., Lim, Pharath, Krogstad, Donald J., Halsey, Eric S., Lucchi, Naomi W., and Koita, Ousmane A.

Published
2021
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4. Imported Cases of Malaria — Puerto Rico, July–October 2015

Author

Dirlikov, Emilio, Rodríguez, Carmen, Morales, Shirley, Martínez, Laura Castro, Mendez, Juan B., Sanchez, Anibal Cruz, Burgos, Jesús Hernández, Santiago, Zobeida, Cuevas-Ruis, Rosa Ivette, Camacho, Sheila Adorno, Mercado, Enid Román, Guzmán, Jessica Falcón, Ryff, Kyle, Luna-Pinto, Carolina, Arguin, Paul M., Chenet, Stella M., Silva-Flannery, Luciana, Ljolje, Dragan, Velázquez, Julio Cadiz, Thomas, Dana, and Garcia, Brenda Rivera

Published
2016

6. Diagnostic Performance of Loop-Mediated Isothermal Amplification and Ultrasensitive Rapid Diagnostic Tests for Malaria Screening Among Pregnant Women in Kenya

Author

Samuels, Aaron M, primary, Towett, Oliver, additional, Seda, Brian, additional, Wiegand, Ryan E, additional, Otieno, Kephas, additional, Chomba, Miriam, additional, Lucchi, Naomi, additional, Ljolje, Dragan, additional, Schneider, Kammerle, additional, Walker, Patrick G T, additional, Kwambai, Titus K, additional, Slutsker, Laurence, additional, ter Kuile, Feiko O, additional, and Kariuki, Simon K, additional

Published
2022
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8. Additional file 1 of Therapeutic efficacy of artemether–lumefantrine and artesunate–amodiaquine for the treatment of uncomplicated Plasmodium falciparum malaria in Mali, 2015–2016

Author

Diarra, Youssouf, Koné, Oumar, Lansana Sangaré, Lassina Doumbia, Dade Bouye Ben Haidara, Mouctar Diallo, Ababacar Maiga, Hamadoun A. Sango, Halidou Sidibé, Mihigo, Jules, Nace, Douglas, Ljolje, Dragan, Talundzic, Eldin, Venkatachalam Udhayakumar, Eckert, Erin, Woodfill, Celia J., Moriarty, Leah F., Pharath Lim, Krogstad, Donald J., Halsey, Eric S., Lucchi, Naomi W., and Koita, Ousmane A.

Subjects
parasitic diseases
Abstract

Additional file 1: Table S1. Kaplan Meier efficacy estimates and hazard ratios between AL and ASAQ, therapeutic efficacy monitoring, Mali, 2015–2016. Table S2. msp1, msp2, and glurp genotyping data (allele sizes are reported in base pairs (bp)).

Published
2021
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9. Therapeutic efficacy of artemether-lumefantrine and artesunate-amodiaquine for the treatment of uncomplicated Plasmodium falciparum malaria in Mali, 2015–2016

Author

Diarra, Youssouf, primary, Koné, Oumar, additional, Sangaré, Lansana, additional, Doumbia, Lassina, additional, Haidara, Dade Bouye Ben, additional, Diallo, Mouctar, additional, Maiga, Ababacar, additional, Sango, Hammadoun Aly, additional, Sidibé, Halidou, additional, Mihigo, Jules, additional, Nace, Douglas, additional, Ljolje, Dragan, additional, Talundzic, Eldin, additional, Udhayakumar, Venkatachalam, additional, Eckert, Erin, additional, Woodfill, Celia Jane, additional, Moriarty, Leah F., additional, Lim, Pharath, additional, Krogstad, Donald John, additional, Halsey, Eric S., additional, Lucchi, Naomi W., additional, and Koita, Ousmane, additional

Published
2020
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11. Artemether–Lumefantrine Efficacy for the Treatment of Uncomplicated Plasmodium falciparum Malaria in Choco, Colombia after 8 Years as First-Line Treatment

Author

Olivera, Mario J., primary, Guerra, Angela Patricia, additional, Cortes, Liliana Jazmin, additional, Horth, Roberta Z., additional, Padilla, Julio, additional, Novoa, Jonathan, additional, Ade, María de la Paz, additional, Ljolje, Dragan, additional, Lucchi, Naomi W., additional, Marquiño, Wilmer, additional, Renteria, Martha, additional, Yurgaky, Wilman, additional, and Macedo de Oliveira, Alexandre, additional

Published
2020
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12. Molecular Characterization of a Cluster of Imported Malaria Cases in Puerto Rico

Author

Paul M. Arguin, Stella M. Chenet, Ljolje Dragan, Brenda Rivera-Garcia, Venkatachalam Udhayakumar, Naomi W. Lucchi, Kimberly E. Mace, Luciana Silva-Flannery, and Emilio Dirlikov

Subjects
Genetic Markers, 0301 basic medicine, Plasmodium falciparum, 030106 microbiology, 030231 tropical medicine, Population, Plasmodium, 03 medical and health sciences, 0302 clinical medicine, Chloroquine, Virology, parasitic diseases, Genotype, medicine, Humans, Malaria, Falciparum, education, Phylogeny, Travel, education.field_of_study, Caribbean island, biology, Puerto Rico, Articles, medicine.disease, biology.organism_classification, Malaria, Infectious Diseases, Genetic marker, Parasitology, medicine.drug
Abstract

The Caribbean island of Hispaniola is targeted for malaria elimination. Currently, this is the only island with ongoing transmission of malaria in the Caribbean. In 2015, six patients from Puerto Rico and one from Massachusetts, who traveled to Punta Cana, Dominican Republic, were confirmed to be infected with Plasmodium falciparum. Additional molecular analysis was performed at the Centers for Disease Control and Prevention to characterize the drug-resistant alleles and Plasmodium population genetic markers. All specimens carried wildtype genotypes for chloroquine, sulfadoxine-pyrimethamine, and artemisinin resistance genetic markers. A mutation in codon 184 (Y/F) of Pfmdr-1 gene was observed in all samples and they shared an identical genetic lineage as determined by microsatellite analysis. This genetic profile was similar to one previously reported from Hispaniola suggesting that a clonal P. falciparum residual parasite population present in Punta Cana is the source population for these imported malaria cases.

Published
2017

13. MOESM1 of Prevalence of molecular markers of artemisinin and lumefantrine resistance among patients with uncomplicated Plasmodium falciparum malaria in three provinces in Angola, 2015

Author

Ljolje, Dragan, Dimbu, Pedro, Kelley, Julia, Goldman, Ira, Nace, Douglas, Aleixo Macaia, Halsey, Eric, Ringwald, Pascal, Fortes, Filomeno, Venkatachalam Udhayakumar, Talundzic, Eldin, Lucchi, Naomi, and Plucinski, Mateusz

Subjects
parasitic diseases
Abstract

Additional file 1. Association between pfk13 and pfmdr1 polymorphisms and late treatment failures observed during therapeutic efficacy studies in Angola, 2015, stratified by treatment, full data.

Published
2018
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15. Screening forPfhrp2/3-DeletedPlasmodium falciparum, Non-falciparum, and Low-Density Malaria Infections by a Multiplex Antigen Assay

Author

Plucinski, Mateusz M, primary, Herman, Camelia, additional, Jones, Sophie, additional, Dimbu, Rafael, additional, Fortes, Filomeno, additional, Ljolje, Dragan, additional, Lucchi, Naomi, additional, Murphy, Sean C, additional, Smith, Nahum T, additional, Cruz, Kurtis R, additional, Seilie, Annette M, additional, Halsey, Eric S, additional, Udhayakumar, Venkatachalam, additional, Aidoo, Michael, additional, and Rogier, Eric, additional

Published
2018
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18. Next-Generation Sequencing and Bioinformatics Protocol for Malaria Drug Resistance Marker Surveillance

Author

Talundzic, Eldin, primary, Ravishankar, Shashidhar, additional, Kelley, Julia, additional, Patel, Dhruviben, additional, Plucinski, Mateusz, additional, Schmedes, Sarah, additional, Ljolje, Dragan, additional, Clemons, Brooke, additional, Madison-Antenucci, Susan, additional, Arguin, Paul M., additional, Lucchi, Naomi W., additional, Vannberg, Fredrik, additional, and Udhayakumar, Venkatachalam, additional

Published
2018
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21. Plasmodium falciparum Drug-Resistant Haplotypes and Population Structure in Postearthquake Haiti, 2010

Author

Morton, Lindsay Carol, primary, Huber, Curtis, additional, Chang, Michelle A., additional, Okoth, Sheila Akinyi, additional, Udhayakumar, Venkatachalam, additional, Griffing, Sean, additional, Barnwell, John W., additional, Lucchi, Naomi, additional, McMorrow, Meredith, additional, Ljolje, Dragan, additional, Boncy, Jacques, additional, Oscar, Roland, additional, and Townes, David, additional

Published
2016
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22. Screening for Pfhrp2/3-Deleted Plasmodium falciparum, Non-falciparum, and Low-Density Malaria Infections by a Multiplex Antigen Assay.

Author

Plucinski, Mateusz M, Herman, Camelia, Jones, Sophie, Dimbu, Rafael, Fortes, Filomeno, Ljolje, Dragan, Lucchi, Naomi, Murphy, Sean C, Smith, Nahum T, Cruz, Kurtis R, Seilie, Annette M, Halsey, Eric S, Udhayakumar, Venkatachalam, Aidoo, Michael, and Rogier, Eric

Subjects
PLASMODIUM falciparum, ANTIGENS, BLOOD sampling, LACTATE dehydrogenase, HISTIDINE
Abstract

Background: Detection of Plasmodium antigens provides evidence of malaria infection status and is the basis for most malaria diagnosis.Methods: We developed a sensitive bead-based multiplex assay for laboratory use, which simultaneously detects pan-Plasmodium aldolase (pAldo), pan-Plasmodium lactate dehydrogenase (pLDH), and P. falciparum histidine-rich protein 2 (PfHRP2) antigens. The assay was validated against purified recombinant antigens, monospecies malaria infections, and noninfected blood samples. To test against samples collected in an endemic setting, Angolan outpatient samples (n = 1267) were assayed.Results: Of 466 Angolan samples positive for at least 1 antigen, the most common antigen profiles were PfHRP2+/pAldo+/pLDH+ (167, 36%), PfHRP2+/pAldo-/pLDH- (163, 35%), and PfHRP2+/pAldo+/pLDH- (129, 28%). Antigen profile was predictive of polymerase chain reaction (PCR) positivity and parasite density. Eight Angolan samples (1.7%) had no or very low PfHRP2 but were positive for 1 or both of the other antigens. PCR analysis confirmed 3 (0.6%) were P. ovale infections and 2 (0.4%) represented P. falciparum parasites lacking Pfhrp2 and/or Pfhrp3.Conclusions: These are the first reports of Pfhrp2/3 deletion mutants in Angola. High-throughput multiplex antigen detection can inexpensively screen for low-density P. falciparum, non-falciparum, and Pfhrp2/3-deleted parasites to provide population-level antigen estimates and identify specimens requiring further molecular characterization. [ABSTRACT FROM AUTHOR]

Published
2019
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23. Notes from the Field: Imported Cases of Malaria — Puerto Rico, July–October 2015

Author

Dirlikov, Emilio, primary, Rodríguez, Carmen, additional, Morales, Shirley, additional, Martínez, Laura Castro, additional, Mendez, Juan B., additional, Sanchez, Anibal Cruz, additional, Burgos, Jesús Hernández, additional, Santiago, Zobeida, additional, Cuevas-Ruis, Rosa Ivette, additional, Camacho, Sheila Adorno, additional, Mercado, Enid Román, additional, Guzmán, Jessica Falcón, additional, Ryff, Kyle, additional, Luna-Pinto, Carolina, additional, Arguin, Paul M., additional, Chenet, Stella M., additional, Silva-Flannery, Luciana, additional, Ljolje, Dragan, additional, Velázquez, Julio Cadiz, additional, Thomas, Dana, additional, and Garcia, Brenda Rivera, additional

Published
2016
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26. PET-PCR method for the molecular detection of malaria parasites in a national malaria surveillance study in Haiti, 2011

Author

Lucchi, Naomi W, primary, Karell, Mara A, additional, Journel, Ito, additional, Rogier, Eric, additional, Goldman, Ira, additional, Ljolje, Dragan, additional, Huber, Curtis, additional, Mace, Kimberly E, additional, Jean, Samuel E, additional, Akom, Eniko E, additional, Oscar, Roland, additional, Buteau, Josiane, additional, Boncy, Jacques, additional, Barnwell, John W, additional, and Udhayakumar, Venkatachalam, additional

Published
2014
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27. Increasing Prevalence of a Novel Triple-Mutant Dihydropteroate Synthase Genotype in Plasmodium falciparumin Western Kenya

Author

Lucchi, Naomi W., Okoth, Sheila Akinyi, Komino, Franklin, Onyona, Philip, Goldman, Ira F., Ljolje, Dragan, Shi, Ya Ping, Barnwell, John W., Udhayakumar, Venkatachalam, and Kariuki, Simon

Abstract

ABSTRACTThe molecular basis of sulfadoxine-pyrimethamine (SP) resistance lies in a combination of single-nucleotide polymorphisms (SNPs) in two genes coding for Plasmodium falciparumdihydrofolate reductase (Pfdhfr) and P. falciparumdihydropteroate synthase (Pfdhps), targeted by pyrimethamine and sulfadoxine, respectively. The continued use of SP for intermittent preventive treatment in pregnant women in many African countries, despite SP's discontinuation as a first-line antimalarial treatment option due to high levels of drug resistance, may further increase the prevalence of SP-resistant parasites and/or lead to the selection of new mutations. An antimalarial drug resistance surveillance study was conducted in western Kenya between 2010 and 2013. A total of 203 clinical samples from children with uncomplicated malaria were genotyped for SNPs associated with SP resistance. The prevalence of the triple-mutant PfdhfrC50I51R59N108I164genotype and the double-mutant PfdhpsS436G437E540A581A613genotype was high. Two triple-mutant Pfdhpsgenotypes, S436G437E540G581A613and H436G437E540A581A613, were found, with the latter thus far being uniquely found in western Kenya. The prevalence of the S436G437E540G581A613genotype was low. However, a steady increase in the prevalence of the Pfdhpstriple-mutant H436G437E540A581A613genotype has been observed since its appearance in early 2000. Isolates with these genotypes shared substantial microsatellite haplotypes with the most common double-mutant allele, suggesting that this triple-mutant allele may have evolved locally. Overall, these findings show that the prevalence of the H436G437E540A581A613triple mutant may be increasing in this population and could compromise the efficacy of SP for intermittent preventive treatment in pregnant women if it increases the resistance threshold further.

Published
2015
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28. Eficacia de la combinación arteméter-lumefantrina para el tratamiento de la malaria no complicada por Plasmodium falciparum, 12 años después de su introducción en Chocó, Colombia.

Author

Javier Olivera, Mario, Patricia Guerra, Ángela, Jazmín Cortés, Liliana, Horth, Roberta, Padilla, Julio, Novoa, Jonathan, de la Paz Ade, María, Lucchi, Naomi Wangui, Ljolje, Dragan, Marquiño, Wilmer, Rentería, Martha, Yurgaky, Wilman, and de Oliveira, Alexandre Macedo

Abstract

Copyright of Revista Biomedica is the property of Centro de Investigaciones Regionales Dr. Hideyo Noguchi; Facultad de Medicina, UADY and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Published
2019

29. Prevalence of molecular markers of artemisinin and lumefantrine resistance among patients with uncomplicated <italic>Plasmodium falciparum</italic> malaria in three provinces in Angola, 2015.

Author

Ljolje, Dragan, Dimbu, Pedro Rafael, Kelley, Julia, Goldman, Ira, Nace, Douglas, Macaia, Aleixo, Halsey, Eric S., Ringwald, Pascal, Fortes, Filomeno, Udhayakumar, Venkatachalam, Talundzic, Eldin, Lucchi, Naomi W., and Plucinski, Mateusz M.

Subjects
ARTEMISININ, PLASMODIUM falciparum, DNA, POLYMERASE chain reaction, HAPLOTYPES, PATIENTS, VACCINATION, THERAPEUTICS
Abstract

Background: Artemisinin-based combination therapy is the first-line anti-malarial treatment for uncomplicated Plasmodium falciparum infection in Angola. To date, the prevalence of polymorphisms in the pfk13 gene, associated with artemisinin resistance, and pfmdr1, associated with lumefantrine resistance, have not been systematically studied in Angola. Methods: DNA was isolated from pretreatment and late treatment failure dried blood spots collected during the 2015 round of therapeutic efficacy studies in Benguela, Lunda Sul, and Zaire Provinces in Angola. The pfk13 propeller domain and pfmdr1 gene were sequenced and analysed for polymorphisms. Pfmdr1 copy number variation was assessed using a real-time PCR method. The association between pfmdr1 and pfk13 mutations and treatment failure was investigated. Results: The majority of pretreatment (99%, 466/469) and all late treatment failure (100%, 50/50) samples were wild type for pfk13. Three of the pretreatment samples (1%) carried the A578S mutation commonly observed in Africa and not associated with artemisinin resistance. All 543 pretreatment and day of late treatment failure samples successfully analysed for pfmdr1 copy number variation carried one copy of pfmdr1. The NYD haplotype was the predominant pfmdr1 haplotype, present in 63% (308/491) of pretreatment samples, followed by NFD, which was present in 32% (157/491) of pretreatment samples. The pfmdr1 N86 allele was overrepresented in day of late treatment failure samples from participants receiving artemether–lumefantrine (p value 0.03). Conclusions: The pretreatment parasites in patients participating in therapeutic efficacy studies in 2015 in Angola’s three sentinel sites showed genetic evidence of susceptibility to artemisinins, consistent with clinical outcome data showing greater than 99% day 3 clearance rates. The lack of increased pfmdr1 copy number is consistent with previous reports from sub-Saharan Africa. Although pfmdr1 NYD and NFD haplotypes were overrepresented in artemether–lumefantrine late treatment failure samples, their role as markers of resistance was unclear given that these haplotypes were also present in the majority of successfully treated patients in the artemether–lumefantrine treatment arms. [ABSTRACT FROM AUTHOR]

Published
2018
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