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14 results on '"Lloret, Alba"'

5. Additional file 1 of Evolutionary origin and functional specialization of Dormancy-Associated MADS box (DAM) proteins in perennial crops

Author

Quesada-Traver, Carles, Lloret, Alba, Carretero-Paulet, Lorenzo, Badenes, María Luisa, and Ríos, Gabino

Abstract

Additional file 1: Fig. S1. Environmental temperature variation corresponding to loquat sampling. Table S1. Plant genomes used in this study. Table S2. Proteins and genes shown in the phylogenetic tree. Table S3. Summary of PpeDAM6 overexpressing Arabidopsis lines. Table S4. FIMO display of motif-8 occurrence in the set of SVP-like proteins. Table S5. Yeast 2-Hybrid (Y2H) interactors. Table S6. Primers used in this study.

Published
2022
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10. Mecanismos de regulación concertada en los procesos de desarrollo y resistencia a estrés en yema de melocotonero

Author

Lloret, Alba, Badenes, María L., and Ríos, Gabino

Subjects
Prunus persica, DAM (DORMANCY-ASSOCIATED MADS-box), Latencia, Sorbitol, Epigenética, F30 Plant genetics and breeding
Abstract

Los árboles frutales han desarrollado un mecanismo de latencia estacional con el fin de evitar el daño por frío en los meristemos. La salida de latencia requiere de la percepción cuantitativa y acumulativa del frío de un modo dependiente del genotipo, dando lugar al carácter denominado ‘necesidades de frío’, clave para la adaptabilidad ambiental de los frutales. Se han identificado genes de melocotonero con expresión diferencial en yemas en distinto estado de latencia. Los genes DAM (DORMANCYASSOCIATED MADS-box) ejercen una función clave en la regulación de la latencia a través de mecanismos epigenéticos. Por otro lado, el gen PpeS6PDH codifica una sorbitol 6-fosfato deshidrogenasa implicada en la síntesis de sorbitol. Se ha postulado la participación del sorbitol en la protección frente al estrés por frío y la desecación en yema latente. Por tanto, y debido a las condiciones climáticas adversas que se suceden durante el invierno, los genes con expresión diferencial en yema de melocotonero no están involucrados únicamente en procesos de latencia, sino que también se relacionan con procesos de resistencia a estrés. Además, también se ha visto que, a pesar de pertenecer a rutas diferentes, comparten mecanismos de regulación epigenéticos. Por lo tanto, existe una regulación concertada entre los procesos de desarrollo de la yema y los procesos de resistencia a estrés.

Published
2018

13. Chromatin-associated regulation of sorbitol synthesis in flower buds of peach

Author

Universitat Politècnica de València. Departamento de Producción Vegetal - Departament de Producció Vegetal, European Social Fund, European Regional Development Fund, Ministerio de Ciencia e Innovación, Institut Valencià d'Investigacions Agràries, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria, Lloret, Alba, Martinez Fuentes, Amparo, Agustí Fonfría, Manuel, Badenes, Maria Luisa, Rios, Gabino, Universitat Politècnica de València. Departamento de Producción Vegetal - Departament de Producció Vegetal, European Social Fund, European Regional Development Fund, Ministerio de Ciencia e Innovación, Institut Valencià d'Investigacions Agràries, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria, Lloret, Alba, Martinez Fuentes, Amparo, Agustí Fonfría, Manuel, Badenes, Maria Luisa, and Rios, Gabino

Abstract

[EN] Key message PpeS6PDH gene is postulated to mediate sorbitol synthesis in flower buds of peach concomitantly with specific chromatin modifications. Abstract Perennial plants have evolved an adaptive mechanism involving protection of meristems within specialized structures named buds in order to survive low temperatures and water deprivation during winter. A seasonal period of dormancy further improves tolerance of buds to environmental stresses through specific mechanisms poorly known at the molecular level. We have shown that peach PpeS6PDH gene is down-regulated in flower buds after dormancy release, concomitantly with changes in the methylation level at specific lysine residues of histone H3 (H3K27 and H3K4) in the chromatin around the translation start site of the gene. PpeS6PDH encodes a NADPH-dependent sorbitol-6-phosphate dehydrogenase, the key enzyme for biosynthesis of sorbitol. Consistently, sorbitol accumulates in dormant buds showing higher PpeS6PDH expression. Moreover, PpeS6PDH gene expression is affected by cold and water deficit stress. Particularly, its expression is up-regulated by low temperature in buds and leaves, whereas desiccation treatment induces PpeS6PDH in buds and represses the gene in leaves. These data reveal the concurrent participation of chromatin modification mechanisms, transcriptional regulation of PpeS6PDH and sorbitol accumulation in flower buds of peach. In addition to its role as a major translocatable photosynthate in Rosaceae species, sorbitol is a widespread compatible solute and cryoprotectant, which suggests its participation in tolerance to environmental stresses in flower buds of peach.

Published
2017

14. Dual regulation of water retention and cell growth by a stress-associated protein (SAP) gene in Prunus

Author

Gabino Ríos, Francisco Gil-Muñoz, María Luisa Badenes, Lorenzo Burgos, Carmen Leida, Cesar Petri, Ana Conejero, Alba Lloret, Lloret, Alba, Conejero, Ana, Leida, Carmen, Petri, César, Gil-Muñoz, Francisco, Burgos, Lorenzo, Badenes, María Luisa, and Ríos, Gabino

Subjects
0106 biological sciences, 0301 basic medicine, Hot Temperature, Transgene, Science, Gene Expression, Vacuole, Biology, 01 natural sciences, Article, Two-Hybrid System Technique, 03 medical and health sciences, Prunus, Gene Expression Regulation, Plant, Osmotic Pressure, Plant Cells, Two-Hybrid System Techniques, Botany, Gene expression, Protein Interaction Mapping, Polyubiquitin, Gene, Cell Proliferation, Plant Proteins, Regulation of gene expression, Prunus persica, Plant Cell, Multidisciplinary, Cell growth, Plant Protein, Water, Plants, Genetically Modified, Cell biology, 030104 developmental biology, Dormancy, Medicine, 010606 plant biology & botany
Abstract

We have identified a gene (PpSAP1) of Prunus persica coding for a stress-associated protein (SAP) containing Zn-finger domains A20 and AN1. SAPs have been described as regulators of the abiotic stress response in plant species, emerging as potential candidates for improvement of stress tolerance in plants. PpSAP1 was highly expressed in leaves and dormant buds, being down-regulated before bud dormancy release. PpSAP1 expression was moderately induced by water stresses and heat in buds. In addition, it was found that PpSAP1 strongly interacts with polyubiquitin proteins in the yeast two-hybrid system. The overexpression of PpSAP1 in transgenic plum plants led to alterations in leaf shape and an increase of water retention under drought stress. Moreover, we established that leaf morphological alterations were concomitant with a reduced cell size and down-regulation of genes involved in cell growth, such as GROWTH-REGULATING FACTOR (GRF)1-like, TONOPLAST INTRINSIC PROTEIN (TIP)-like, and TARGET OF RAPAMYCIN (TOR)-like. Especially, the inverse expression pattern of PpSAP1 and TOR-like in transgenic plum and peach buds suggests a role of PpSAP1 in cell expansion through the regulation of TOR pathway.

Published
2017

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