Search

Your search keyword '"López Andarias, Javier"' showing total 166 results
Start Over Author "López Andarias, Javier"
166 results on '"López Andarias, Javier"'

2. Technical insights into fluorescence lifetime microscopy of mechanosensitive Flipper probes

Author

Roffay, Chloé, primary, García-Arcos, Juan Manuel, additional, Chapuis, Pierrik, additional, López-Andarias, Javier, additional, Schneider, Falk, additional, Colom, Adai, additional, Tomba, Caterina, additional, Meglio, Ilaria Di, additional, Dunsing, Valentin, additional, Matile, Stefan, additional, Roux, Aurélien, additional, and Mercier, Vincent, additional

Published
2022
Full Text
View/download PDF

7. Flipper Probes for the Community

Author

Assies, Lea, primary, García-Calvo, José, additional, Piazzolla, Francesca, additional, Sanchez, Samantha, additional, Kato, Takehiro, additional, Reymond, Luc, additional, Goujon, Antoine, additional, Colom, Adai, additional, López-Andarias, Javier, additional, Straková, Karolína, additional, Mahecic, Dora, additional, Mercier, Vincent, additional, Riggi, Margot, additional, Jiménez-Rojo, Noemi, additional, Roffay, Chloé, additional, Licari, Giuseppe, additional, Tsemperouli, Maria, additional, Neuhaus, Frederik, additional, Fürstenberg, Alexandre, additional, Vauthey, Eric, additional, Hoogendoorn, Sascha, additional, Gonzalez-Gaitan, Marcos, additional, Zumbuehl, Andreas, additional, Sugihara, Kaori, additional, Gruenberg, Jean, additional, Riezman, Howard, additional, Loewith, Robbie, additional, Manley, Suliana, additional, Roux, Aurelien, additional, Winssinger, Nicolas, additional, Sakai, Naomi, additional, Pitsch, Stefan, additional, and Matile, Stefan, additional

Published
2021
Full Text
View/download PDF

9. Genetically Encoded Supramolecular Targeting of Fluorescent Membrane Tension Probes within Live Cells: Precisely Localized Controlled Release by External Chemical Stimulation

Author

López-Andarias, Javier, Straková, Karolína, Martinent, Rémi, Jiménez-Rojo, Noemi, Riezman, Howard, Sakai, Naomi, and Matile, Stefan

Subjects
Chemistry, fluorescent probes, ddc:540, streptavidin, mechanochemistry, mechanobiology, QD1-999, Article, spatiotemporal control, secretory pathway
Abstract

To image membrane tension in selected membranes of interest (MOI) inside living systems, the field of mechanobiology requires increasingly elaborated small-molecule chemical tools. We have recently introduced HaloFlipper, i.e., a mechanosensitive flipper probe that can localize in the MOI using HaloTag technology to report local membrane tension changes using fluorescence lifetime imaging microscopy. However, the linker tethering the probe to HaloTag hampers the lateral diffusion of the probe in all the lipid domains of the MOI. For a more global membrane tension measurement in any MOI, we present here a supramolecular chemistry strategy for selective localization and controlled release of flipper into the MOI, using a genetically encoded supramolecular tag. SupraFlippers, functionalized with a desthiobiotin ligand, can selectively accumulate in the organelle having expressed streptavidin. The addition of biotin as a biocompatible external stimulus with a higher affinity for Sav triggers the release of the probe, which spontaneously partitions into the MOI. Freed in the lumen of endoplasmic reticulum (ER), SupraFlippers report the membrane orders along the secretory pathway from the ER over the Golgi apparatus to the plasma membrane. Kinetics of the process are governed by both the probe release and the transport through lipid domains. The concentration of biotin can control the former, while the expression level of a transmembrane protein (Sec12) involved in the stimulation of the vesicular transport from ER to Golgi influences the latter. Finally, the generation of a cell-penetrating and fully functional Sav-flipper complex using cyclic oligochalcogenide (COC) transporters allows us to combine the SupraFlipper strategy and HaloTag technology.

Published
2021

10. Flipper Probes for the Community

Author

Assies, Lea, García-Calvo, José, Piazzolla, Francesca, Sánchez, Samantha, Kato, Takehiro, Reymond, Luc, Goujon, Antoine, Colom, Adai, López-Andarias, Javier, Straková, Karolína, Mahecic, Dora, Mercier, Vincent, Riggi, Margot, Jiménez-Rojo, Noemí, Roffay, Chloé, Licari, Giuseppe, Tsemperouli, Maria, Neuhaus, Frederik, Fürstenberg, Alexandre, Vauthey, Eric, Hoogendoorn, Sascha, González-Gaitán, Marcos, Zumbuehl, Andreas, Sugihara, Kaori, Gruenberg, Jean, Riezman, Howard, Loewith, Robbie, Manley, Suliana, Roux, Aurélien, Winssinger, Nicolas, Sakai, Naomi, Pitsch, Stefan, Matile, Stefan, Assies, Lea, García-Calvo, José, Piazzolla, Francesca, Sánchez, Samantha, Kato, Takehiro, Reymond, Luc, Goujon, Antoine, Colom, Adai, López-Andarias, Javier, Straková, Karolína, Mahecic, Dora, Mercier, Vincent, Riggi, Margot, Jiménez-Rojo, Noemí, Roffay, Chloé, Licari, Giuseppe, Tsemperouli, Maria, Neuhaus, Frederik, Fürstenberg, Alexandre, Vauthey, Eric, Hoogendoorn, Sascha, González-Gaitán, Marcos, Zumbuehl, Andreas, Sugihara, Kaori, Gruenberg, Jean, Riezman, Howard, Loewith, Robbie, Manley, Suliana, Roux, Aurélien, Winssinger, Nicolas, Sakai, Naomi, Pitsch, Stefan, and Matile, Stefan

Abstract

This article describes four fluorescent membrane tension probes that have been designed, synthesized, evaluated, commercialized and applied to current biology challenges in the context of the NCCR Chemical Biology. Their names are Flipper-TR®, ER Flipper-TR®, Lyso Flipper-TR®, and Mito Flipper-TR®. They are available from Spirochrome.

Published
2021

11. Thiol-Mediated Uptake

Author

Laurent, Quentin, primary, Martinent, Rémi, additional, Lim, Bumhee, additional, Pham, Anh-Tuan, additional, Kato, Takehiro, additional, López-Andarias, Javier, additional, Sakai, Naomi, additional, and Matile, Stefan, additional

Published
2021
Full Text
View/download PDF

12. Protein-directed crystalline 2D fullerene assemblies

Author

30273709, Liutkus, Mantas, López-Andarias, Alicia, Mejías, H, Sara, López-Andarias, Javier, Gil-Carton, David, Feixas, Ferran, Osuna, Sílvia, Matsuda, Wakana, Sakurai, Tsuneaki, Seki, Shu, Atienza, Carmen, Martín, Nazario, Cortajarena, L. Aitziber, 30273709, Liutkus, Mantas, López-Andarias, Alicia, Mejías, H, Sara, López-Andarias, Javier, Gil-Carton, David, Feixas, Ferran, Osuna, Sílvia, Matsuda, Wakana, Sakurai, Tsuneaki, Seki, Shu, Atienza, Carmen, Martín, Nazario, and Cortajarena, L. Aitziber

Abstract

Water soluble 2D crystalline monolayers of fullerenes grow on planar assemblies of engineered consensus tetratricopeptide repeat proteins. Designed fullerene-coordinating tyrosine clamps on the protein introduce specific fullerene binding sites, which facilitate fullerene nucleation. Through reciprocal interactions between the components, the hybrid material assembles into two-dimensional 2 nm thick structures with crystalline order, that conduct photo-generated charges. Thus, the protein-fullerene hybrid material is a demonstration of the developments toward functional materials with protein-based precision control of functional elements.

Published
2020

13. Inhibitors of thiol-mediated uptake

Author

Cheng, Yangyang, primary, Pham, Anh-Tuan, additional, Kato, Takehiro, additional, Lim, Bumhee, additional, Moreau, Dimitri, additional, López-Andarias, Javier, additional, Zong, Lili, additional, Sakai, Naomi, additional, and Matile, Stefan, additional

Published
2021
Full Text
View/download PDF

17. Inhibitors of Thiol-Mediated Uptake

Author

Cheng, Yangyang, primary, Pham, Anh-Tuan, primary, Kato, Takehiro, primary, Lim, Bumhee, primary, Moreau, Dimitri, primary, López-Andarias, Javier, primary, Zong, Lili, primary, Sakai, Naomi, primary, and Matile, Stefan, primary

Published
2020
Full Text
View/download PDF

20. Cell-Penetrating Streptavidin: A General Tool for Bifunctional Delivery with Spatiotemporal Control, Mediated by Transport Systems Such as Adaptive Benzopolysulfane Networks

Author

López-Andarias, Javier, primary, Saarbach, Jacques, additional, Moreau, Dimitri, additional, Cheng, Yangyang, additional, Derivery, Emmanuel, additional, Laurent, Quentin, additional, González-Gaitán, Marcos, additional, Winssinger, Nicolas, additional, Sakai, Naomi, additional, and Matile, Stefan, additional

Published
2020
Full Text
View/download PDF

21. Protein-directed crystalline 2D fullerene assemblies

Author

Liutkus, Mantas, primary, López-Andarias, Alicia, additional, Mejías, Sara H., additional, López-Andarias, Javier, additional, Gil-Carton, David, additional, Feixas, Ferran, additional, Osuna, Sílvia, additional, Matsuda, Wakana, additional, Sakurai, Tsuneaki, additional, Seki, Shu, additional, Atienza, Carmen, additional, Martín, Nazario, additional, and Cortajarena, Aitziber L., additional

Published
2020
Full Text
View/download PDF

22. Photocleavable Fluorescent Membrane Tension Probes: Fast Release with Spatiotemporal Control in Inner Leaflets of Plasma Membrane, Nuclear Envelope, and Secretory Pathway.

Author

López‐Andarias, Javier, Eblighatian, Krikor, Pasquer, Quentin T. L., Assies, Lea, Sakai, Naomi, Hoogendoorn, Sascha, and Matile, Stefan

Subjects
*NUCLEAR membranes, *CELL membranes, *PAMPHLETS, *BIOLOGICAL systems, *SPECIAL effects in lighting
Abstract

Mechanosensitive flipper probes are attracting interest as fluorescent reporters of membrane order and tension in biological systems. We introduce PhotoFlippers, which contain a photocleavable linker and an ultralong tether between mechanophore and various targeting motifs. Upon irradiation, the original probe is released and labels the most ordered membrane that is accessible by intermembrane transfer. Spatiotemporal control from photocleavable flippers is essential to access open, dynamic or elusive membrane motifs without chemical or physical interference. For instance, fast release with light is shown to place the original small‐molecule probes into the innermost leaflet of the nuclear envelope to image changes in membrane tension, at specific points in time of membrane trafficking along the secretory pathway, or in the inner leaflet of the plasma membrane to explore membrane asymmetry. These results identify PhotoFlippers as useful chemistry tools to enable research in biology. [ABSTRACT FROM AUTHOR]

Published
2022
Full Text
View/download PDF

23. Anion–π Catalysis on Carbon Nanotubes

Author

Bornhof, Anna‐Bea, primary, Vázquez‐Nakagawa, Mikiko, additional, Rodríguez‐Pérez, Laura, additional, Ángeles Herranz, María, additional, Sakai, Naomi, additional, Martín, Nazario, additional, Matile, Stefan, additional, and López‐Andarias, Javier, additional

Published
2019
Full Text
View/download PDF

24. Protein-Directed Crystalline 2D Fullerene Assemblies

Author

LIUTKUS, MANTAS, primary, López-Andarias, Alicia, primary, Mejías, Sara H., primary, López-Andarias, Javier, primary, Gil-Carton, David, primary, Feixas, Ferran, primary, Osuna, Sílvia, primary, Matsuda, Wakana, primary, Sakurai, Tsuneaki, primary, Seki, Shu, primary, Atienza, Carmen, primary, Martín, Nazario, primary, and Lopez Cortajarena, Aitziber, primary

Published
2019
Full Text
View/download PDF

27. Primary Anion−π Catalysis and Autocatalysis

Author

Zhang, Xiang, primary, Hao, Xiaoyu, additional, Liu, Le, additional, Pham, Anh-Tuan, additional, López-Andarias, Javier, additional, Frontera, Antonio, additional, Sakai, Naomi, additional, and Matile, Stefan, additional

Published
2018
Full Text
View/download PDF

28. The Emergence of Anion−π Catalysis

Author

Zhao, Yingjie, primary, Cotelle, Yoann, additional, Liu, Le, additional, López-Andarias, Javier, additional, Bornhof, Anna-Bea, additional, Akamatsu, Masaaki, additional, Sakai, Naomi, additional, and Matile, Stefan, additional

Published
2018
Full Text
View/download PDF

36. Bio-inspired hierarchical organization of electroactive supramolecular architectures based on carbon nanoestructures

Author

López Andarias, Javier, Martín León, Nazario, and Atienza Castellanos, Carmen María

Subjects
Química orgánica
Abstract

La nanotecnología, referida a la manipulación de la materia a nivel molecular y supramolecular, es un área emergente con prometedores beneficios para la humanidad.1 En particular, las nanoestructuras de carbono han captado gran atención debido a sus atractivas propiedades electrónicas y mecánicas. Imitar la naturaleza mediante el desarrollo de procedimientos bioinspirados puede ser una interesante estrategia para organizar nanomateriales ricos en carbono con el fin de maximizar sus propiedades optoelectrónicas...

Published
2015

37. Repeat protein scaffolds: ordering photo- and electroactive molecules in solution and solid state

Author

Mejías, Sara H., López Andarias, Javier, Sakurai, Tsuneaki, Yoneda, Satoru, Erazo, Kevin P., Seki, Shu, Atienza, Carmena, Martín, Nazario, Cortajarena, Aitziber L., Mejías, Sara H., López Andarias, Javier, Sakurai, Tsuneaki, Yoneda, Satoru, Erazo, Kevin P., Seki, Shu, Atienza, Carmena, Martín, Nazario, and Cortajarena, Aitziber L.

Abstract

The precise control over the organization of photoactive components at the nanoscale is one of the main challenges for the generation of new and sophisticated macroscopically ordered materials with enhanced properties. In this work we present a novel bioinspired approach using protein-based building blocks for the arrangement of photo and electroactive porphyrin derivatives. We used a designed repeat protein scaffold with demonstrated unique features that allow for the control of their structure, functionality, and assembly. Our designed domains act as exact biomolecular templates to organize porphyrin molecules at the required distance. The hybrid conjugates retain the structure and assembly properties of the protein scaffold and display the spectroscopic features of orderly aggregated porphyrins along the protein structure. Finally,we achieved a solid ordered bio-organic hybrid thin film with anisotropic photoconductivity., Unión Europea. FP7, Ministerio de Economía y Competitividad (MINECO), European Research Council, Depto. de Química Orgánica, Fac. de Ciencias Químicas, TRUE, pub

Published
2016

38. Bio-inspired hierarchical organization of electroactive supramolecular architectures based on carbon nanoestructures

Author

Martín León, Nazario, Atienza Castellanos, Carmen María, López Andarias, Javier, Martín León, Nazario, Atienza Castellanos, Carmen María, and López Andarias, Javier

Abstract

La nanotecnología, referida a la manipulación de la materia a nivel molecular y supramolecular, es un área emergente con prometedores beneficios para la humanidad.1 En particular, las nanoestructuras de carbono han captado gran atención debido a sus atractivas propiedades electrónicas y mecánicas. Imitar la naturaleza mediante el desarrollo de procedimientos bioinspirados puede ser una interesante estrategia para organizar nanomateriales ricos en carbono con el fin de maximizar sus propiedades optoelectrónicas..., Nanotechnology, referring as the manipulation of matter at the molecular and supramolecular scale, is an emerging area with promising benefits for humanity.1 In particular, carbon nanostructures have received a great attention due to their exciting electronic and mechanical properties. Mimicking nature, that is, developing bioinspired procedures, could be one of the most intelligent strategies to organize matter, in particular carbon-rich nanostructures, with the aim of maximizing their fabulous optoelectronic properties...

Published
2016

41. Electron-deficient fullerenes in triple-channel photosystems

Author

López Andarias, Javier, Bolag, Altan, Nançoz, Christoph, Vauthey, Eric, Atienza, Carmen, Sakai, Naomi, Martín, Nazario, Matile, Stefan, López Andarias, Javier, Bolag, Altan, Nançoz, Christoph, Vauthey, Eric, Atienza, Carmen, Sakai, Naomi, Martín, Nazario, and Matile, Stefan

Abstract

Fullerenes of increasing electron deficiency are designed, synthesized and evaluated in multicomponent surface architectures to ultimately build gradients in LUMO levels with nine components over 350 mV down to -4.22 eV., Ministerio de Economía y Competitividad (MINECO), European Research Council, Comunidad de Madrid, Depto. de Química Orgánica, Fac. de Ciencias Químicas, TRUE, pub

Published
2015

42. Toward Bioelectronic Nanomaterials: Photoconductivity in Protein–Porphyrin Hybrids Wrapped around SWCNT.

Author

López‐Andarias, Javier, Atienza, Carmen, Martín, Nazario, Mejías, Sara H., Cortajarena, Aitziber L., Sakurai, Tsuneaki, Matsuda, Wakana, Seki, Shu, Feixas, Ferran, and Osuna, Sílvia

Subjects
*NANOSTRUCTURED materials, *BIOELECTRONICS, *SINGLE walled carbon nanotubes, *PHOTOCONDUCTIVITY, *PORPHYRINS, *PROTEINS
Abstract

Abstract: The development of sophisticated ordered functional materials is one of the important challenges in current science. One of the keys to enhance the properties of these materials is the control of the organization and morphology at different scales. This work presents a novel bioinspired methodology to achieve highly ordered donor/acceptor bio‐nanohybrids using a designed repeat protein as scaffold, endowed with photoactive and electron donating porphyrin (P) units, to efficiently wrap around electron accepting single wall carbon nanotubes (SWCNT). A systematic experimental and theoretical study to evaluate the effect of the length of the protein reveals that longer proteins wrap around the SWCNT in a more efficient manner due to the stronger supramolecular interaction existing between the inner concave surface of the protein (namely Trp and His residues) and the convex surface of the (7,6)‐SWCNT. Interestingly, spectroscopy and X‐ray diffraction data further confirm that the so‐called protein‐P–SWCNT donor–acceptor bio‐nanohybrids retain the original protein structure. Finally, the new bio‐nanohybrids show a remarkable enhancement on the photoconductivity values by flash‐photolysis microwave conductivity (FP‐TRMC technique) demonstrating that the major charge carriers of electrons are injected into the SWCNTs and move along the 1D‐structures. [ABSTRACT FROM AUTHOR]

Published
2018
Full Text
View/download PDF

43. Anion‐<italic>π</italic> Catalysis: Focus on Nonadjacent Stereocenters.

Author

Zhang, Xiang, Liu, Le, López‐Andarias, Javier, Wang, Chao, Sakai, Naomi, and Matile, Stefan

Abstract

Anion‐π interactions have been recently introduced to catalysis with the idea to stabilize anionic intermediates on π‐acidic surfaces. Realized examples include enolate, enamine and iminium chemistry, domino processes and Diels–Alder reactions. Moving on from the formation of contiguous stereogenic centers on π‐acidic surfaces, herein we report the first asymmetric anion‐π catalysis of cascade reactions that afford nonadjacent stereocenters. Conjugate addition‐protonation of achiral disubstituted enolate donors to 2‐chloroacrylonitrile generates 1,3‐nonadjacent stereocenters with moderate enantioselectivity and diastereoselectivity. The explored catalysts operate with complementary naphthalenediimide and fullerene surfaces with highly positive quadrupole moments and high polarizability, respectively, and proximal amine bases. We find that anion‐π catalysts can increase the diastereoselectivity of the reaction beyond the maximal 1:4.0 dr with conventional catalysts to maximal 5.3:1 dr on the large fullerene surfaces. The enantioselectivity of anion‐π catalysts, best on the confined naphthalenediimide surfaces with strong quadrupole moment, exceed the performance of conventional catalysts except for comparable results with a new, most compact, surprisingly powerful bifunctional control catalyst. Simultaneously increased rates and stereoselectivities compared to control catalysts without π‐acidic surface support that contributions of anion‐π interactions to the catalytic cascade process are significant. [ABSTRACT FROM AUTHOR]

Published
2018
Full Text
View/download PDF

46. Anion-π Catalysis on Fullerenes.

Author

López-Andarias, Javier, Matile, Stefan, and Frontera, Antonio

Subjects
*FULLERENES, *ANIONS, *DIELS-Alder reaction, *ENANTIOSELECTIVE catalysis, *COMPUTER simulation
Abstract

Anion-π interactions on fullerenes are about as poorly explored as the use of fullerenes in catalysis. However, strong exchange-correlation contributions and the localized π holes on their surface promise unique selectivities. To elaborate on this promise, tertiary amines are attached nearby. Dependent on their positioning, the resulting stabilization of anionic transition states on fullerenes is shown to accelerate disfavored enolate addition and exo Diels-Alder reactions enantioselectively. The found selectivities are consistent with computational simulations, particularly concerning the discrimination of differently planarized and charge-delocalized enolate tautomers by anion-π interactions. Enolate-π interactions on fullerenes are much shorter than standard π-π interactions and anion-π interactions on planar surfaces, and alternative cation-π interactions are not observed. These findings open new perspectives with regard to anion-π interactions in general and the use of carbon allotropes in catalysis. [ABSTRACT FROM AUTHOR]

Published
2017
Full Text
View/download PDF

47. Catalysis with Chalcogen Bonds.

Author

Benz, Sebastian, López ‐ Andarias, Javier, Mareda, Jiri, Sakai, Naomi, and Matile, Stefan

Subjects
*CHALCOGENS, *CHEMICAL bonds, *CATALYSIS, *HYDROGENATION, *QUINOLINE, *THIOPHENES, *ANIONS
Abstract

Herein, we introduce catalysts that operate with chalcogen bonds. Compared to conventional hydrogen bonds, chalcogen bonds are similar in strength but more directional and hydrophobic, thus ideal for precision catalysis in apolar solvents. For the transfer hydrogenation of quinolines and imines, rate enhancements well beyond a factor of 1000 are obtained with chalcogen bonds. Better activities with deeper σ holes and wider bite angles, chloride inhibition and correlation with computed anion binding energies are consistent with operational chalcogen bonds. Comparable to classics, such as 2,2′-bipyrroles or 2,2′-bipyridines, dithieno[3,2-b;2′,3′-d]thiophenes (DTTs), particularly their diimides, but also wide-angle cyclopentadithiazole-4-ones are identified as privileged motifs to stabilize transition states in the focal point of the σ holes on their two co-facial endocyclic sulfur atoms. [ABSTRACT FROM AUTHOR]

Published
2017
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results