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2 results on '"Lochmann, B"'

1. Premature ejaculation: results from a five-country European observational study

Author

Giuliano, F, Patrick, Dl, Porst, H, La Pera, G, Kokoszka, A, Merchant, S, Rothman, M, Gagnon, Dd, Polverejan, E, Behre H, 3004 Study G. r. o. u. p., Bondil, P, Chevallier, D, Ciesielska, B, Colson, Mh, Corman, A, Cuzin, B, Darewicz, B, Dean, Jd, Demailly, M, Depko, A, Dulko, S, Eardley, I, Frajese, G, Gentile, V, Gontero, Paolo, Granata, Ar, Hackett, Gi, Harten, J, Hermabessiere, J, Jankowska Wojniak, I, Kell, P, Kula, K, Lenzi, A, Lew Starowicz, Z, Lochmann, B, Maroni, J, Mimoun, S, Montorsi, F, Muzzonigro, G, Pawa, R, Ralph, Dj, Schrödter, A, Smoliński, R, Stahl, Hd, Szelazek, G, Szymula, S, Wilcke, D, Wieczerzak, Z, and Wyczolkowski, M.

Published
2008

2. Histone H3 localizes to the centromeric DNA in budding yeast.

Author

Lochmann B and Ivanov D

Subjects
Centromere chemistry, Chromatin Immunoprecipitation methods, DNA chemistry, DNA genetics, Gene Expression Regulation, Fungal, Kinetochores chemistry, Kinetochores metabolism, Multiprotein Complexes chemistry, Multiprotein Complexes genetics, Nucleosomes genetics, Protein Binding genetics, Centromere genetics, Chromosomal Proteins, Non-Histone genetics, Chromosomal Proteins, Non-Histone metabolism, Chromosomes, Fungal genetics, DNA-Binding Proteins genetics, DNA-Binding Proteins metabolism, Histones genetics, Histones metabolism, Saccharomyces cerevisiae genetics, Saccharomyces cerevisiae Proteins genetics, Saccharomyces cerevisiae Proteins metabolism
Abstract

During cell division, segregation of sister chromatids to daughter cells is achieved by the poleward pulling force of microtubules, which attach to the chromatids by means of a multiprotein complex, the kinetochore. Kinetochores assemble at the centromeric DNA organized by specialized centromeric nucleosomes. In contrast to other eukaryotes, which typically have large repetitive centromeric regions, budding yeast CEN DNA is defined by a 125 bp sequence and assembles a single centromeric nucleosome. In budding yeast, as well as in other eukaryotes, the Cse4 histone variant (known in vertebrates as CENP-A) is believed to substitute for histone H3 at the centromeric nucleosome. However, the exact composition of the CEN nucleosome remains a subject of debate. We report the use of a novel ChIP approach to reveal the composition of the centromeric nucleosome and its localization on CEN DNA in budding yeast. Surprisingly, we observed a strong interaction of H3, as well as Cse4, H4, H2A, and H2B, but not histone chaperone Scm3 (HJURP in human) with the centromeric DNA. H3 localizes to centromeric DNA at all stages of the cell cycle. Using a sequential ChIP approach, we could demonstrate the co-occupancy of H3 and Cse4 at the CEN DNA. Our results favor a H3-Cse4 heterotypic octamer at the budding yeast centromere. Whether or not our model is correct, any future model will have to account for the stable association of histone H3 with the centromeric DNA., Competing Interests: The authors have declared that no competing interests exist.

Published
2012
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