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Your search keyword '"Loenhout, Marijn"' showing total 13 results
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13 results on '"Loenhout, Marijn"'

7. Single-cell analysis identifies a CD33+subset of human cord blood cells with high regenerative potential

Author

Knapp, David, Hammond, Colin, Hui, Tony, Loenhout, Marijn, Wang, Fangwu, Aghaeepour, Nima, Miller, Paul, Moksa, Michelle, Rabu, Gabrielle, Beer, Philip, Pellacani, Davide, Humphries, R., Hansen, Carl, Hirst, Martin, and Eaves, Connie

Abstract

Elucidation of the identity and diversity of mechanisms that sustain long-term human blood cell production remains an important challenge. Previous studies indicate that, in adult mice, this property is vested in cells identified uniquely by their ability to clonally regenerate detectable, albeit highly variable levels and types, of mature blood cells in serially transplanted recipients. From a multi-parameter analysis of the molecular features of very primitive human cord blood cells that display long-term cell outputs in vitro and in immunodeficient mice, we identified a prospectively separable CD33+CD34+CD38−CD45RA−CD90+CD49f+phenotype with serially transplantable, but diverse, cell output profiles. Single-cell measurements of the mitogenic response, and the transcriptional, DNA methylation and 40-protein content of this and closely related phenotypes revealed subtle but consistent differences both within and between each subset. These results suggest that multiple regulatory mechanisms combine to maintain different cell output activities of human blood cell precursors with high regenerative potential. Knapp et al. analyse the heterogeneous molecular profiles and functions of CD49f human cord blood haematopoietic stem cells and report that a subset with CD33 expression has improved regenerative activity.

Published
2018
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10. Highly Parallel Magnetic Tweezers for the Study of DNA-Protein Interactions

Author

Burnham, Daniel R., primary, De Vlaminck, Iwijn, additional, Henighan, Thomas, additional, van Loenhout, Marijn T.J., additional, Pfeiffer, Indriati, additional, Huijts, Julius, additional, Kerssemakers, Jacob, additional, van Langen-Suurling, Anja, additional, van der Drift, Emil, additional, Wyman, Claire, additional, and Dekker, Cees, additional

Published
2012
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11. Highly Parallel Magnetic Tweezers by Targeted DNA Tethering

Author

De Vlaminck, Iwijn, primary, Henighan, Thomas, additional, van Loenhout, Marijn T.J., additional, Pfeiffer, Indriati, additional, Huijts, Julius, additional, Kerssemakers, Jacob W. J., additional, Katan, Allard J., additional, van Langen-Suurling, Anja, additional, van der Drift, Emile, additional, Wyman, Claire, additional, and Dekker, Cees, additional

Published
2011
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12. Annealing Helicase HARP: A Single Molecule Study

Author

De Vlaminck, Iwijn, primary, Yusufzai, Timur, additional, van Loenhout, Marijn T.J., additional, Vidic, Iztok, additional, Kanaar, Roland, additional, Lebbink, Joyce H.G., additional, Kadonaga, James T., additional, and Dekker, Cees, additional

Published
2011
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