1. CBFA2T3-GLIS2 model of pediatric acute megakaryoblastic leukemia identifies FOLR1 as a CAR T cell target
- Author
-
Le, Quy, Hadland, Brandon, Smith, Jenny L, Leonti, Amanda, Huang, Benjamin J, Ries, Rhonda, Hylkema, Tiffany A, Castro, Sommer, Tang, Thao T, McKay, Cyd N, Perkins, LaKeisha, Pardo, Laura, Sarthy, Jay, Beckman, Amy K, Williams, Robin, Idemmili, Rhonda, Furlan, Scott, Ishida, Takashi, Call, Lindsey, Srivastava, Shivani, Loeb, Anisha M, Milano, Filippo, Imren, Suzan, Morris, Shelli M, Pakiam, Fiona, Olson, James M, Loken, Michael R, Brodersen, Lisa Eidenschink, Riddell, Stanley R, Tarlock, Katherine, Bernstein, Irwin D, Loeb, Keith R, and Meshinchi, Soheil
- Subjects
Biomedical and Clinical Sciences ,Oncology and Carcinogenesis ,Immunology ,Pediatric Research Initiative ,Pediatric ,Cancer ,Genetics ,Pediatric Cancer ,Stem Cell Research ,Stem Cell Research - Nonembryonic - Human ,Stem Cell Research - Nonembryonic - Non-Human ,Biotechnology ,Hematology ,Rare Diseases ,Childhood Leukemia ,Orphan Drug ,2.1 Biological and endogenous factors ,Aetiology ,Animals ,Child ,Child ,Preschool ,Humans ,Infant ,Disease Models ,Animal ,Folate Receptor 1 ,Immunotherapy ,Adoptive ,Leukemia ,Megakaryoblastic ,Acute ,Oncogene Proteins ,Fusion ,T-Lymphocytes ,Transcriptome ,Xenograft Model Antitumor Assays ,Cancer immunotherapy ,Leukemias ,Oncogenes ,Oncology ,Therapeutics ,Medical and Health Sciences ,Biological sciences ,Biomedical and clinical sciences ,Health sciences - Abstract
The CBFA2T3-GLIS2 (C/G) fusion is a product of a cryptic translocation primarily seen in infants and early childhood and is associated with dismal outcome. Here, we demonstrate that the expression of the C/G oncogenic fusion protein promotes the transformation of human cord blood hematopoietic stem and progenitor cells (CB HSPCs) in an endothelial cell coculture system that recapitulates the transcriptome, morphology, and immunophenotype of C/G acute myeloid leukemia (AML) and induces highly aggressive leukemia in xenograft models. Interrogating the transcriptome of C/G-CB cells and primary C/G AML identified a library of C/G-fusion-specific genes that are potential targets for therapy. We developed chimeric antigen receptor (CAR) T cells directed against one of the targets, folate receptor α (FOLR1), and demonstrated their preclinical efficacy against C/G AML using in vitro and xenograft models. FOLR1 is also expressed in renal and pulmonary epithelium, raising concerns for toxicity that must be addressed for the clinical application of this therapy. Our findings underscore the role of the endothelial niche in promoting leukemic transformation of C/G-transduced CB HSPCs. Furthermore, this work has broad implications for studies of leukemogenesis applicable to a variety of oncogenic fusion-driven pediatric leukemias, providing a robust and tractable model system to characterize the molecular mechanisms of leukemogenesis and identify biomarkers for disease diagnosis and targets for therapy.
- Published
- 2022