1. Vanadate, an inhibitor of tyrosine phosphatases, induced premature anaphase in oocytes and aneuploidy and polyploidy in mouse bone marrow cells.
- Author
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Mailhes JB, Hilliard C, Fuseler JW, and London SN
- Subjects
- Aneuploidy, Animals, Cytogenetic Analysis, Dose-Response Relationship, Drug, Female, Mice, Mice, Inbred ICR, Mutagenesis, Polyploidy, Anaphase drug effects, Aneugens toxicity, Bone Marrow Cells drug effects, Chromosome Aberrations chemically induced, Enzyme Inhibitors toxicity, Oocytes drug effects, Protein Tyrosine Phosphatases antagonists & inhibitors, Vanadates toxicity
- Abstract
Protein tyrosine phosphatases are needed for activating maturation promoting factor, meiotic spindle assembly and spindle checkpoint inactivation. The protein phosphatase inhibitor vanadate was used to upset the kinase-phosphatase equilibrium during oocyte maturation (OM) and the metaphase anaphase transition (MAT) prior to cytogenetic analyses of mouse oocytes and bone marrow cells. ICR females received pregnant mare serum gonadotrophin (PMSG) and 48h later received human chorionic gonadotrophin (hCG). Vanadate doses of 0, 5, 15, and 25mg/kg were administered intraperitoneally immediately after hCG and ovulated oocytes and bone marrow cells were processed for cytogenetic analyses 18h after hCG. Data were analyzed by Chi-square and Fisher's exact tests. Vanadate induced different cytogenetic abnormalities in oocytes and in bone marrow cells. The frequencies of oocytes exhibiting premature anaphase (spontaneous activation) in vanadate exposed mice were significantly (P<0.01) elevated over controls; whereas, in bone marrow cells, the levels of tetraploidy, hyperploidy and premature centromere separation were significantly (P<0.01) increased by vanadate treatment. These results suggest that alteration of the kinase-phosphatase equilibrium during OM and the MAT leads to cytogenetic abnormalities that differ between oocytes and bone marrow cells.
- Published
- 2003
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