1. ZBP-89 reduces the cell death threshold in hepatocellular carcinoma cells by increasing caspase-6 and S phase cell cycle arrest
- Author
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King Yip Fung, Art Tessier, George G. Chen, Long Chuan Bai, Paul B.S. Lai, Ann K.Y. To, Ursula P. F. Chan, and Juanita L. Merchant
- Subjects
Cancer Research ,Programmed cell death ,Carcinoma, Hepatocellular ,Antineoplastic Agents ,Caspase 6 ,Biology ,Article ,S Phase ,Flow cytometry ,Cell Line, Tumor ,medicine ,Carcinoma ,Humans ,Staurosporine ,Transcription factor ,Cell Death ,medicine.diagnostic_test ,Cell Cycle ,Liver Neoplasms ,Cell cycle ,Flow Cytometry ,medicine.disease ,Molecular biology ,digestive system diseases ,DNA-Binding Proteins ,Oncology ,Cell culture ,Fluorouracil ,Transcription Factors ,medicine.drug - Abstract
ZBP-89 inhibits the some tumor cells but its role in HCC is unknown. We investigated effect of ZBP-89 on cell death of 5 HCC cell lines with different status of p53. We found that ZBP-89 significantly induced cell death of all HCC cells particularly those with wild-type p53. The inhibition was well correlated with the induction of caspase-6 activity. The inhibition of caspase-6 abolished the effect of ZBP-89. ZBP-89 reduced the cells in G2-M but increased them in S phase. With the changes in caspase-6 and cell cycle, ZBP-89 greatly enhanced the killing effectiveness of 5-fluorouracil or staurosporine in HCC cells.
- Published
- 2009