Search

Your search keyword '"Longo O"' showing total 243 results
Start Over Author "Longo O"
243 results on '"Longo O"'

9. Bonifica dei Terreni Contaminati. Osservazioni critiche, linee guida e proposte normative

Author

Aggazzotti, Gabriella, Andretta, Alfonso, Benezzoli, Umberto, Bonifazi, Giuseppe, Butti, Luciano, Cappucci, Sergio, Davini, Andrea, Nauta, Stefano Di, Ferrante, Margherita, Finotelli, Fabrizio, Longo, Oronzo Antonio, Miano, Teodoro, Moretto, Carlo Giovanni, Orrico, Bruno, Pedron, Roberto, Peres, Federico, Pivato, Alberto, Quercia, Francesca, Racciatti, Rocco Virginio, Braschi, Ilaria, Bonifica dei terreni contaminati, in: Aggazzotti, G., Ajmone Marsan, F., Andretta, A., Andrisani, A. G., Angeloni, A., Arcella, C., Ardissone, E., Arena, U., Argese, E., Balestreri, A., Bavestrelli, A., Benezzoli, U., Bergamini, M., Bonifazi, G., Braschi, I., Bratti, A., Brutti, R., Butti, L., Canè, G., Cappucci, S., Castellano, G., Cervelli, M., Cianciullo, A., Cossu, R., Davini, A., Davit, J. P., Davoli, E., Dei Svaldi, M., Del Frate, A. A., Di Nauta, S., Di Sante, M., Dotti, M., Evangelista, R., Faleschini, F., Farina, R., Ferrante, M., Finotelli, F., Fogal, E., Forni, A., Forni, F., Furnari, S., Gnocchi, A., Gosso, E., Grisolia, M., Guerini, A., Lagorio, M., Lattanzi, P., Lavagnolo, M. C., Leombruni, A., Longo, O. A., Malagoli, M., Marotta, A., Massara, R., Massarini, P., Mastrantonio, M., Mazzieri, F., Miano, T., Misano, V., Monfroni, I., Morello, L., Moretto, C. G., Orrico, B., Parma, M., Pasqualini, E., Pedron, R., Peres, F., Petruzzelli, D., Petruzzelli, G. A., Piccolo, A., Pingitore, D., Pintore, M., Piscitelli, M., Pivato, A., Potenza, F., Potenza, G., Preda, G., Quercia, F., Racciati, R. V., Ranieri, G., Ranieri, M., Recchia, L., Ricci, R., Righini, L., Russo, P., Scalco, R., Schiavone, C., Spagni, A., Tasselli, L., Tonello, P., Tresso, G., Trevisi, V. M., Ursino, N., Vecchio, A., and Vinci, L.

Published
2015

10. Site-directed mutagenesis on the translation elongation factors from the archaeon Sulfolobus solfataricus

Author

Raimo, Gennaro, Arcari, P., Cantiello, P., Castellano, I., DE VENDITTIS, E., Lamberti, A., Longo, O., Ruocco, M. R., AND MASULLO, M., FARINA B., FARAONE MENNELLA M.R., Raimo, G., Arcari, Paolo, Cantiello, P., Castellano, I., DE VENDITTIS, Emmanuele, Lamberti, Annalisa, Longo, O., Ruocco, MARIA ROSARIA, and Masullo, M.

Subjects
archaeon, Elongation factor, ribosome dependent GTPase, endogenous GTPase, Elongation factors, Sulfolobus solfataricu, mutagenic analysis, hyperthermophile
Abstract

The present article reviews our recent work carried out on the translation elongation factors EF-1alpha, EF-2 and EF-1beta isolated from the hyperthermophilic archaeon Sulfolobus solfataricus. During the elongation cycle the GTPase activity of SsEF-1alpha and SsEF-2 and the nucleotide exchange activity of SsEF-1beta are crucial reactions ensuring the efficiency and the unidirectionality of the entire protein synthesis process. The site-directed mutagenesis carried out on the first consensus motif [G/A]XXXXGK of SsEF-1alpha and SsEF-2 allowed to assess the key role played by the alternate presence of G or A in the first position of this sequence motif. In particular, the data reported indicate that the presence of glycine triggers the intrinsic GTPase of both elongation factors, whereas alanine keeps this activity to a minimum level. The mutagenic analysis of a conserved valine faced to the above mentioned glycine in SsEF-1alpha showed its involvement in the interaction with aa-tRNA and in the stability of the enzyme. Even in S. solfataricus the nucleotide exchange activity of SsEF-1beta is essential to recycle the active GTP-bound form of SsEF-1alpha. However, some relevant structural and functional differences emerge, when comparing the properties of SsEF-1beta with those of eubacterial and eukaryal functional analogues. The smaller size of SsEF-1beta and its transient interaction with the SsEF-1alpha-GDP complex represent distinctive properties of the archaeal exchange factor. In conclusion, the data reported indicate that the general scheme of the elongation cycle is conserved also in the hyperthermophilic source analysed, even though the structural properties and the intermediate reactions catalysed by the elongation factors may be slightly different in different species.

Published
2004

11. Interaction of archaeal translation elongation factors with eubacterial protein synthesis inhibitors

Author

Cantiello, P., Castellano, I., DE VENDITTIS, E., Lamberti, A., Longo, O., Masullo, Mariorosario, Raimo, G., Ruocco, M. R., Arcari, P., Cantiello, P, Castellano, I, DE VENDITTIS, Emmanuele, Lamberti, Annalisa, Longo, O, Masullo, M, Raimo, G, Ruocco, MARIA ROSARIA, and Arcari, Paolo

Subjects
inhibitor, protein synthesi, Elongation factor, Sulfolobus solfataricu, hyperthermophile
Abstract

This review describes some features of the detailed structure-function relationship in two extremophilic model proteins, the translation elongation factors SsEF–1α and SsEF–2 isolated from the hyperthermophilic archaeon Sulfolobus solfataricus. The data reported describe the effect of typical inhibitors of the corresponding eubacterial EF–Tu and EF–G on the multiple reactions supported by SsEF–1α and SsEF–2 during the fulfilment of their functions. The comparison with the properties of other bacterial or eukaryal factors allows a philogenetic relationship of the peptide elongation cycle in the three living domains. SsEF–1α is able to interact with typical eubacterial protein synthesis inhibitors, such as the antibiotic kirromycin and GE2270A. In particular, kirromycin improves the intrinsic GTPase of some mutant forms of the archaeal factor, whereas GE2270A inhibits both the intrinsic and the ribosome–dependent GTPase of wild–type SsEF–1α. The analysis of the behaviour of engineered forms of the archaeal factor proved that GE2270A interacts mainly with the middle domain of SsEF–1α. Also SsEF–2 interacts with typical inhibitors of the eubacterial EF–G. In fact, the steroid antibiotics fusidic and helvolic acid are able to inhibit poly(Phe) synthesis and the ribosome–dependent GTPase of SsEF–2. A mutant form of SsEF–2 exhibited an increased sensitivity to these antibiotics, thus allowing to detect a fusidane responsiveness even in the absence of ribosome. This latter feature could indicate that the archaeal SsEF–2 harbors the structural requirements for the interaction with fusidane antibiotics.

Published
2004

17. Therapeutic immunization with hiv-1 tat reduces immune activation and loss of regulatory t-cells and improves immune function in subjects on HAART

Author

Ensoli, B., Bellino, S., Tripiciano, A., Longo, O., Francavilla, V., Marcotullio, S., Cafaro, A., Picconi, O., Paniccia, G., Scoglio, A., Arancio, A., Ariola, C., Ruiz Alvarez, M. J., Campagna, M., Scaramuzzi, D., Iori, C., Esposito, R., Mussini, C., Ghinelli, F., Sighinolfi, L., Palamara, G., Latini, A., Angarano, G., Ladisa, N., Soscia, F., Mercurio, V. S., Lazzarin, A., Tambussi, G., Visintini, R., Mazzotta, F., Di Pietro, M., Galli, M., Rusconi, S., Carosi, G., Torti, C., Di Perri, G., Bonora, S., Ensoli, F., Garaci, E., and Segala, D.

Subjects
CD4-Positive T-Lymphocytes, Male, T-Lymphocytes, HIV Infections, CD38, CD8-Positive T-Lymphocytes, Lymphocyte Activation, T-Lymphocytes, Regulatory, Antiretroviral Therapy, Highly Active, vaccine, Cytotoxic T cell, Homeostasis, Killer Cells, Prospective Studies, tat, Virology/Vaccines, AIDS Vaccines, B-Lymphocytes, Multidisciplinary, HIV, AIDS, Nausea, Infectious Diseases/HIV Infection and AIDS, Middle Aged, Settore MED/07 - Microbiologia e Microbiologia Clinica, Combined Modality Therapy, Regulatory, Killer Cells, Natural, medicine.anatomical_structure, Treatment Outcome, Virology/Immunodeficiency Viruses, Natural, Medicine, tat Gene Products, Human Immunodeficiency Virus, Female, Adult, Aged, Asthenia, HIV-1, Humans, Immunization, Cell activation, tat Gene Products, Human Immunodeficiency Virus, Research Article, Science, T cell, Antiretroviral Therapy, Pathology/Immunology, Biology, NO, Immune system, Antigen, Immunology/Immunity to Infections, medicine, Highly Active, Virology, Immunology, CD8
Abstract

UnlabelledAlthough HAART suppresses HIV replication, it is often unable to restore immune homeostasis. Consequently, non-AIDS-defining diseases are increasingly seen in treated individuals. This is attributed to persistent virus expression in reservoirs and to cell activation. Of note, in CD4(+) T cells and monocyte-macrophages of virologically-suppressed individuals, there is continued expression of multi-spliced transcripts encoding HIV regulatory proteins. Among them, Tat is essential for virus gene expression and replication, either in primary infection or for virus reactivation during HAART, when Tat is expressed, released extracellularly and exerts, on both the virus and the immune system, effects that contribute to disease maintenance. Here we report results of an ad hoc exploratory interim analysis (up to 48 weeks) on 87 virologically-suppressed HAART-treated individuals enrolled in a phase II randomized open-label multicentric clinical trial of therapeutic immunization with Tat (ISS T-002). Eighty-eight virologically-suppressed HAART-treated individuals, enrolled in a parallel prospective observational study at the same sites (ISS OBS T-002), served for intergroup comparison. Immunization with Tat was safe, induced durable immune responses, and modified the pattern of CD4(+) and CD8(+) cellular activation (CD38 and HLA-DR) together with reduction of biochemical activation markers and persistent increases of regulatory T cells. This was accompanied by a progressive increment of CD4(+) T cells and B cells with reduction of CD8(+) T cells and NK cells, which were independent from the type of antiretroviral regimen. Increase in central and effector memory and reduction in terminally-differentiated effector memory CD4(+) and CD8(+) T cells were accompanied by increases of CD4(+) and CD8(+) T cell responses against Env and recall antigens. Of note, more immune-compromised individuals experienced greater therapeutic effects. In contrast, these changes were opposite, absent or partial in the OBS population. These findings support the use of Tat immunization to intensify HAART efficacy and to restore immune homeostasis.Trial registrationClinicalTrials.gov NCT00751595.

Published
2010

18. The presence of anti-Tat antibodies in HIV-infected individuals is associated with containment of CD4+ T-cell decay and viral load, and with delay of disease progression: Results of a 3-year cohort study

Author

Bellino, S, Tripiciano, A, Picconi, O, Francavilla, V, Longo, O, Sgadari, C, Paniccia, G, Arancio, A, Angarano, G, Ladisa, N, Lazzarin, A, Tambussi, G, Nozza, S, Torti, C, Focà, E, Palamara, G, Latini, A, Sighinolfi, L, Mazzotta, F, Di Pietro, M, Di Perri, G, Bonora, S, Mercurio, V, Mussini, C, Gori, A, Galli, M, Monini, P, Cafaro, A, Ensoli, F, Ensoli, B, Ensoli, B., GORI, ANDREA, Bellino, S, Tripiciano, A, Picconi, O, Francavilla, V, Longo, O, Sgadari, C, Paniccia, G, Arancio, A, Angarano, G, Ladisa, N, Lazzarin, A, Tambussi, G, Nozza, S, Torti, C, Focà, E, Palamara, G, Latini, A, Sighinolfi, L, Mazzotta, F, Di Pietro, M, Di Perri, G, Bonora, S, Mercurio, V, Mussini, C, Gori, A, Galli, M, Monini, P, Cafaro, A, Ensoli, F, Ensoli, B, Ensoli, B., and GORI, ANDREA

Abstract

Background: Tat is a key HIV-1 virulence factor, which plays pivotal roles in virus gene expression, replication, transmission and disease progression. After release, extracellular Tat accumulates in tissues and exerts effects on both the virus and the immune system, promoting immune activation and virus spreading while disabling the host immune defense. In particular, Tat binds Env spikes on virus particles forming a virus entry complex, which favors infection of dendritic cells and efficient transmission to T cells via RGD-binding integrins. Tat also shields the CCR5-binding sites of Env rendering ineffective virus neutralization by anti-Env antibodies (Abs). This is reversed by the anti-Tat Abs present in natural infection or induced by vaccination.Findings: Here we present the results of a cohort study, showing that the presence of anti-Tat Abs in asymptomatic and treatment-naïve HIV-infected subjects is associated with containment of CD4+ T-cell loss and viral load and with a delay of disease progression. In fact, no subjects with high anti-Tat Ab titers initiated antiretroviral therapy during the three years of follow-up. In contrast, no significant effects were seen for anti-Env and anti-Gag Abs. The increase of anti-Env Ab titers was associated with a reduced risk of starting therapy only in the presence of anti-Tat Abs, suggesting an effect of combined anti-Tat and anti-Env Abs on the Tat/Env virus entry complex and on virus neutralization.Conclusions: Anti-Tat immunity may help delay HIV disease progression, thus, targeting Tat may offer a novel therapeutic intervention to postpone antiretroviral treatment or to increase its efficacy. © 2014 Bellino et al.; licensee BioMed Central Ltd

Published
2014

19. Functional role of translation Elongation Factor 1A in IFNa-induced apoptosis of human lung cancer cells

Author

Annalisa Lamberti, Caraglia, M., Longo, O., Sanges, C., Marra, M., Abbruzzese, A., Arcari, P., Lamberti, Annalisa, Michele, Caraglia, Olimpia, Longo, Sanges, Carmen, Monica, Marra, Alberto, Abbruzzese, and Arcari, Paolo

Subjects
protein synthesi, Translation elongation factor 1A, ubiquitin, interferon a
Abstract

An increasing number of evidences suggest the involvement of the eukaryotic elongation factor 1A, a core component of the protein synthesis machinery, at the onset of cell transformation. In fact, eEF1A is shown to be up-regulated in cell death; moreover, it seems to be involved in the regulation of ubiquitin-mediated protein degradation. In addition, eEF1A undergoes several post-translational modifications, mainly phosphorylation and methylation, that generally influence the activity of the protein. This article summarizes the present knowledges on the several extra-translational roles of eEF1A also in order to understand as the protein synthesis regulatory mechanisms could offer tools for cancer intervention.

Published
2007

26. Porous silicon optical sensors for vapors, liquids, and biological molecules

Author

De Stefano L, Rendina I, Moretti L, Rossi AM, Lamberti A, Longo O, and Arcari P

Subjects
Physics::Optics
Abstract

Recently, an increasing interest has been devoted to the use of porous silicon (p-Si) in photonics and in sensing fields. In particular, the great reactivity, mainly due to its large surface to volume ratio, has demonstrated to be promising in sensing applications for the detection of gases, vapors, and biochemical molecules. In this work, we present experimental and numerical results on p-Si optical microcavities as sensing transducers in biological and chemical fields. The measures are based on the change of the cavity reflectivity spectrum induced by the exposition to the bio-chemical specimen under test. The p-Si microcavity has a Fabry-Perot structure confined between two Distributed Bragg Reflectors (DBRs) with high reflectivity in the wavelength range of interest.

Published
2003

31. HIV-1 Tat Promotes Integrin-Mediated HIV Transmission to Dendritic Cells by Binding Env Spikes and Competes Neutralization by Anti-HIV Antibodies

Author

Sommer, P., Monini, P., Cafaro, A., Srivastava, I. K., Moretti, S., Sharma, V. A., Andreini, Claudia, Chiozzini, C., Ferrantelli, F., Pavone Cossut, M. R., Tripiciano, A., Nappi, F., Longo, O., Bellino, S., Picconi, O., Fanales Belasio, E., Borsetti, A., Toschi, E., Schiavoni, I., Bacigalupo, I., Kan, E., Sernicola, L., Maggiorella, M. T., Montin, K., Porcu, M., Leone, P., Collacchi, B., Palladino, C., Ridolfi, B., Falchi, M., Macchia, I., Ulmer, J. B., Buttò, S., Sgadari, C., Magnani, M., Federico, M. P. M., Titti, F., Banci, Lucia, Dallocchio, F., Rappuoli, R., Ensoli, F., Barnett, S. W., Garaci, E., and Ensoli, B.

Subjects
Male, Integrins, T-Lymphocytes, viruses, lcsh:Medicine, HIV Infections, HIV Antibodies, HIV Envelope Protein gp120, V3 loop, Virus Replication, Biochemistry, Neutralization, Epitope, Molecular Cell Biology, HIV vaccine, lcsh:Science, IMMUNODEFICIENCY-VIRUS TYPE-1, FIBROBLAST-GROWTH-FACTOR, PROTEIN-PROTEIN DOCKING, CYNOMOLGUS MONKEYS, ENVELOPE PROTEIN, KAPOSIS-SARCOMA, STRUCTURAL CHARACTERIZATION, THERAPEUTIC VACCINES, PARTIAL DELETION, GP120 CORE, AIDS Vaccines, Multidisciplinary, Vaccination, env Gene Products, Human Immunodeficiency Virus, virus diseases, Recombinant Proteins, Extracellular Matrix, Molecular Docking Simulation, AIDS, Cytochemistry, Medicine, Infectious diseases, tat Gene Products, Human Immunodeficiency Virus, Antibody, Oligopeptides, Protein Binding, Research Article, Receptors, CXCR4, Viral Entry, Receptors, CCR5, Clinical Research Design, Preclinical Models, HIV prevention, Sexually Transmitted Diseases, Retrovirology and HIV immunopathogenesis, Viral diseases, Biology, Microbiology, Viral Attachment, Virus, Immune Deficiency, Neutralization Tests, Viral entry, Virology, Vaccine Development, Cell Adhesion, Animals, Humans, Protein Interaction Domains and Motifs, Vaccines, Virus-Like Particle, Immunity to Infections, Binding Sites, lcsh:R, Immunity, HIV, Viral Vaccines, TAT, Dendritic Cells, Virus Internalization, Antibodies, Neutralizing, Macaca fascicularis, Viral replication, HIV-1, biology.protein, lcsh:Q, Clinical Immunology, Viral Transmission and Infection
Abstract

Use of Env in HIV vaccine development has been disappointing. Here we show that, in the presence of a biologically active Tat subunit vaccine, a trimeric Env protein prevents in monkeys virus spread from the portal of entry to regional lymph nodes. This appears to be due to specific interactions between Tat and Env spikes that form a novel virus entry complex favoring R5 or X4 virus entry and productive infection of dendritic cells (DCs) via an integrin-mediated pathway. These Tat effects do not require Tat-transactivation activity and are blocked by anti-integrin antibodies (Abs). Productive DC infection promoted by Tat is associated with a highly efficient virus transmission to T cells. In the Tat/Env complex the cysteine-rich region of Tat engages the Env V3 loop, whereas the Tat RGD sequence remains free and directs the virus to integrins present on DCs. V2 loop deletion, which unshields the CCR5 binding region of Env, increases Tat/Env complex stability. Of note, binding of Tat to Env abolishes neutralization of Env entry or infection of DCs by anti-HIV sera lacking anti-Tat Abs, which are seldom present in natural infection. This is reversed, and neutralization further enhanced, by HIV sera containing anti-Tat Abs such as those from asymptomatic or Tat-vaccinated patients, or by sera from the Tat/Env vaccinated monkeys. Thus, both anti-Tat and anti-Env Abs are required for efficient HIV neutralization. These data suggest that the Tat/Env interaction increases HIV acquisition and spreading, as a mechanism evolved by the virus to escape anti-Env neutralizing Abs. This may explain the low effectiveness of Env-based vaccines, which are also unlikely to elicit Abs against new Env epitopes exposed by the Tat/Env interaction. As Tat also binds Envs from different clades, new vaccine strategies should exploit the Tat/Env interaction for both preventative and therapeutic interventions.

Published
2012
Full Text
View/download PDF

32. Parallel Conduction of the Phase I Preventive and Therapeutic Trials Based on the Tat Vaccine Candidate

Author

Bellino, S., primary, Francavilla, V., additional, Longo, O., additional, Tripiciano, A., additional, Paniccia, G., additional, Arancio, A., additional, Fiorelli, V., additional, Scoglio, A., additional, Collacchi, B., additional, Campagna, M., additional, Lazzarin, A., additional, Tambussi, G., additional, Din, C., additional, Visintini, R., additional, Narciso, P., additional, Antinori, A., additional, D'Offizi, G., additional, Giulianelli, M., additional, Carta, M., additional, Di Carlo, A., additional, Palamara, G., additional, Giuliani, M., additional, Laguardia, M., additional, Monini, P., additional, Magnani, M., additional, Ensoli, F., additional, and Ensoli, B., additional

Published
2009
Full Text
View/download PDF

34. C-Raf antagonizes apoptosis induced by IFN-α in human lung cancer cells by phosphorylation and increase of the intracellular content of elongation factor 1A

Author

Lamberti, A, primary, Longo, O, additional, Marra, M, additional, Tagliaferri, P, additional, Bismuto, E, additional, Fiengo, A, additional, Viscomi, C, additional, Budillon, A, additional, Rapp, U R, additional, Wang, E, additional, Venuta, S, additional, Abbruzzese, A, additional, Arcari, P, additional, and Caraglia, M, additional

Published
2007
Full Text
View/download PDF

38. Optical sensors for vapors, liquids, and biological molecules based on porous silicon technology.

Author

De Stefano, L., Moretti, L., Lamberti, A., Longo, O., Rocchia, M., Rossi, A.M., Arcari, P., and Rendina, I.

Abstract

The sensing of chemicals and biochemical molecules using several porous silicon optical microsensors, based both on single-layer interferometers and resonant-cavity-enhanced microstructures, is reported. The operation of both families of sensors is based on the variation of the average refractive index of the porous silicon region, due to the interaction with chemical substances either in vapor or liquid state, which results in marked shifts of the device reflectivity spectra. The well established single-layer configuration has been used to test a new chemical approach based on Si-C bonds for covalent immobilization of biological molecules, as probe, in a stable way on the porous silicon surface. Preliminary results on complementary oligonucleotide recognition, based on this technique, are also presented and discussed. Porous silicon optical microcavities, based on multilayered resonating structures, have been used to detect chemical substances and, in particular, flammable and toxic organic solvents, and some hydrocarbons. The results put in evidence the high sensitivity, the reusability, and the low response time of the resonant-cavity-enhanced sensing technique. The possibility of operating at room temperature, of remote interrogation, and the absence of electrical contacts are further advantages characterizing the sensing technique. [ABSTRACT FROM PUBLISHER]

Published
2004
Full Text
View/download PDF

43. C-Raf antagonizes apoptosis induced by INF-a in human lung cancer cells by phosphorylation and increase of the intracellular content of elongation factor 1A

Author

Eugenia Wang, Salvatore Venuta, M. Marra, Ulf R. Rapp, Alberto Abbruzzese, A Fiengo, Pierosandro Tagliaferri, Alfredo Budillon, Olimpia Longo, Caterina Viscomi, Ettore Bismuto, Michele Caraglia, Annalisa Lamberti, Paolo Arcari, Lamberti, Annalisa, Longo, O, Marra, M, Tagliaferri, P, Bismuto, E, Fiengo, A, Viscomi, C, Budillon, A, Rapp, U. P. WANG E, Venuta, S, Abbruzzese, A, Arcari, Paolo, Caraglia, M., Lamberti, A., Longo, O., Marra, M., Taglaferri, P., Bismuto, E., Fiengo, A., Viscomi, C., Budillon, A., Rapp, U., Wang, E., Venuta, S., ABBRUZZESE SACCARDI, A., Arcari, P., and Caraglia, Michele

Subjects
Proteasome Endopeptidase Complex, Programmed cell death, Lung Neoplasms, Alpha interferon, Apoptosis, Biology, Phosphoserine, Peptide Elongation Factor 1, Epidermal growth factor, Cell Line, Tumor, Humans, Immunoprecipitation, c-Raf, Phosphorylation, RNA, Small Interfering, Kinase activity, Molecular Biology, Oncogene Proteins, apoptosis, Ubiquitin, Interferon-alpha, EF-1A, Cell Biology, Transfection, Molecular biology, Cell biology, Proto-Oncogene Proteins c-raf, C-Raf, Protein Transport, C-Raf, EF-1A, IFNα, apoptosis, proteasome, epidermoid cancer cells, Phosphothreonine, IFN-alpha, Protein Processing, Post-Translational, proteasome, Protein Binding
Abstract

Interferon alpha (IFNalpha) induces both apoptosis and a counteracting epidermal growth factor Erk-dependent survival response in cancer cells. In this report, IFNalpha increased eukaryotic elongation factor 1A (eEF-1A) protein expression by inhibition of eEF-1A degradation via a proteasome-dependent pathway. The reduction of the expression level of eEF-1A by RNA interference enhanced the apoptosis induced by IFNalpha on the same cells. Moreover, IFNalpha induced the phosphorylation of both serine and threonine in eEF-1A. These effects were paralleled by an increased co-immunoprecipitation and colocalization of eEF-1A with C-Raf. The suppression of C-Raf kinase activity with the inhibitor BAY 43-9006 completely antagonized the increase of both eEF-1A phosphorylation and expression and of C-Raf/eEF-1A colocalization induced by IFNalpha and enhanced apoptosis and eEF-1A ubiquitination. Cell transfection with the mutated K48R ubiquitin increased EF-1A expression and desensitized tumor cells to the modulating effects of IFNalpha. The dynamic simulation of 3Dstructure of eEF-1A identified putative serine and threonine phosphorylation sites. In conclusion, the interaction between eEF-1A and C-Raf increases eEF-1A stability and induces a survival activity.

Published
2007

44. Il sistema dei confini

Author

SBACCHI, Michele, Dalzero, S, Iorio, A, Longo, O, Pirina, C, Rugino, S, Sigurtà, D, and SBACCHI, Michele

Subjects
Boundary, system, design, limit, Settore ICAR/14 - Composizione Architettonica E Urbana
Abstract

As far as architecture is concerned, boundaries are not standing-alone entities. They rather act in systems. This is of paramount importance when looking at the complex overlapping of different and heterogeneous boundaries within physical space. The paper emphasizes and focus on this issue, using some architectural theoretical speculations over centuries as well as examples taken from art and philosophy.

Published
2020

45. CONFINI E PROGETTO PER LO SPAZIO PUBBLICO A TREVISO

Author

LEONE, Manfredi, PIAZZESE, Cristina, Dalzero, S, Iorio, A, Longo, O, Pirina, C, Rugino, S, Sigurtà, D, Albrecht, A, Albrighettoni, J, Battaino, C, Battaino, V, Camerin, F, Cavalieri, C, Cervieri, G, Condello, A, Coricelli, F, Da Boit, C, Saviane, G, De Curtis, A, Fabris, LMF, Ferlenga, A, Ferrari, M, Fossati, P, Gaeta, L, Ingersoll, R, Leone, M, Martinelli, E, Minuta, U, Morpurgo, G, Nunes, J, Occelli, CLM, Palma, R, Pappalardo, S, Passamani, I, Peghin, G, Pintacuda, L, Quendolo, A, Rispoli, M, Rubio-Garrido, A, Ruzzon, D, Sbacchi, M, Zecchin, L, LEONE, Manfredi, and PIAZZESE, Cristina

Subjects
Settore ICAR/15 - Architettura Del Paesaggio, landscape, boundaries, design, territory, architecture, public space, paesaggio, confini, progetto, territorio, architettura, spazio pubblico
Abstract

In questa prima parte del Ventunesimo secolo assistiamo alla presenza nelle comunità urbane di attori non istituzionali, soggetti non canonici che, in qualche modo, si presentano sulla scena del governo urbano per sopperire alla mancanza di quelle azioni amministrative che sarebbe invece il dovere naturale degli amministratori della città. Un filo conduttore collega Palermo e Treviso dal 2018, un parallelismo che riunisce rispettivamente l’esperienza di Parco Uditore e Prato della Fiera. Due realtà diverse per storia, geografia, dimensioni, struttura, ma con alcune somiglianze nell’azione svolta da parti delle loro comunità, in uno scenario di riappropriazione e partecipazione attiva, proprio di quei rappresentanti della cittadinanza che non smettono mai di osservare il territorio e hanno proposto per la sua sopravvivenza azioni concrete di sviluppo, protezione, valorizzazione. In questo breve saggio vogliamo parlare di un’esperienza di spazio In this first part of the 21st century we witness the presence in the urban communities of non-institutional actors, non-canonical subjects that, in some way, do they present on the urban government scene to make up for the lack of those actions administrative which would instead be the natural duty of city administrators. A wire conductor connects Palermo and Treviso since 2018, a parallelism that brings together respectively the experience of Parco Auditore and Prato della Fiera. Two realities different in history, geography, dimensions, structure, but with some similarities in the action carried out by parts of them community, in a scenario of reappropriation and active participation, just of those citizenship representatives who never stop observing the area and have proposed for its survival concrete actions of development, protection, enhancement. In this short essay we want to talk about a rediscovered public space experience, a place of possible expectations, a scenario of possible future.

Published
2020

46. Linearità e waterfront

Author

Sbacchi, M., Longo, O, and Sbacchi, Michele

Subjects
Settore ICAR/14 - Composizione Architettonica E Urbana, linea, linearità
Abstract

Il waterfront è un luogo particolare sia per la città che per il progetto di architettura. Esistono due modi per intenderlo: uno perpendicolare ad esso ed uno orizzontale. Il primo è quello più tradizionale e scontato, il secondo invece meno diffuso ma di forte interesse.

Published
2018

47. Articoli 131, 132 e 133

Author

Patroni Griffi Andrea, Commenti di A. Morrone, D. Tega, M. D’Amico, M. Cavino, G. Rivosecchi, F. Palermo, A. Pin, F. Alicino, G. Repetto, P. Bonetti, A. Guazzarotti, T. Groppi. - Parte prima. Diritti e doveri dei cittadini. - Titolo I. Rapporti civili. Commenti di C. Martinelli, M. Cecchetti, M. Orofino, L. Castelli, F. Rosa, F. Clementi, I. Ruggiu, P. Tanzarella, G.E. Vigevani, F.G. Pizzetti, D. Morana, F. Dal Canto, N. Pignatelli, G. Martinico, R. Calvano, A. Cardone. - Titolo II. Rapporti etico-sociali. Commenti di F. Biondi, G. Matucci, A. Sperti, G. Scaccia, A. Iannuzzi, M. Benvenuti. - Titolo III. Rapporti economici. Commenti di E. Ferioli, M. Della Morte, C. Tripodina, G. Arconzo, A. Celotto, L. Lorello, L. Cassetti, Q. Camerlengo, F. Furlan, E. Mostacci, F. Pizzolato e F. Mattassoglio, G. Pistorio, R. Manfrellotti. - Titolo IV. Rapporti politici. Commenti di M. Rubechi, S. Curreri, L. Trucco, G. Tarli Barbieri, T.F. Giupponi, S. Sileoni, A. Morelli. - Parte seconda. Ordinamento della Repubblica. - Titolo I. Il Parlamento. Commenti di N. Lupo, E. Cavasino, E. Gianfrancesco, S. Leone, L. Scaffardi, S. Sileoni, E. Mostacci, G. Romeo, A. Mastromarino, F. Biondi, I. Ciolli, M. Cosulich, C. Martinelli, M. Cuniberti, P.L. Petrillo, G. Piccirilli, M. Plutino, P. Passaglia, F. Dal Canto, I. Pellizzone, F. Rosa, F. Ghera, G. Tarli Barbieri, A. Vedaschi, E. Longo, O. Pollicino, T.F. Giupponi, G.G. Carboni. - Titolo II. Il Presidente della Repubblica. Commenti di F. Clementi, F. Furlan, G. Grasso, M. Cavino, M. Olivetti, F. Pastore, M. Oliviero e M.C. Locchi. - Titolo III. Il Governo. Commenti di C. Bassu, J.O. Frosini, C. Bologna, P. Milazzo, P. Veronesi, A. Valastro e N. Pettinari, M. Di Folco, P. Bonetti. - Titolo IV. La Magistratura. Commenti di E. Bindi, F.G. Pizzetti, G. Arconzo, G. Ferri, A. Deffenu, P. Martino, A. Apostoli, A. Pin, S. Catalano, A. Baraggia, G. Sorrenti, S. Pajno. - Titolo V. Le Regioni, le Province, i Comuni. Commenti di F. Fabrizzi, A. Morelli, S. Ninatti e G. Tiberi, L. Cuocolo, D. Morana, M. Massa, M. Caielli, E.C. Raffiotta, G. Rivosecchi, M. Cecchetti, G. Fontana, D. Coduti, M. Belletti, G. D’Alessandro, S. Pajno, A. Sterpa, S. Calzolaio, A. Patroni Griffi. - Titolo VI. Garanzie costituzionali. Commenti di G. D’Amico, D. Tega, E. Lamarque, V. Marcenò, Q. Camerlengo, A. Buratti. - Disposizioni transitorie e finali. Commento di P. Scarlatti., Clementi, Cuocolo, Rosa, Vigevani, and PATRONI GRIFFI, Andrea

Subjects
Regioni italiane, modifiche territoriali, articolo 131 Costituzione, articolo 132 Costituzione, articolo 133 Costituzione
Published
2018

48. Paesaggio, architettura e trasformazioni urbane: scelte consapevoli per il terzo millennio

Author

Leone, M., LONGO, O, AA.VV., and LEONE, Manfredi

Subjects
Settore ICAR/15 - Architettura Del Paesaggio, Paesaggio, Architettura, Natura, Citta', Trasformazione
Abstract

Il tema del rapporto tra architettura e natura e’ stato occasione secolare di esplorazione e ricerca. Nelle sue declinazioni piu recenti questo rapporto ha osservato una sempre maggiore integrazione tra concetti che possono apparire antinomici. Il bisogno di Natura in Architettura ottiene una consacrazione con il pensiero di Le Corbusier: il tetto-giardino prima, esplicitazione della integrazione della natura in architettura, che mette sull’edificio moderno la dimensione naturale normalmente destinata al livello del giardino. Successivamente gli studi sulla citta’ moderna e la sua integrazione con i sistemi naturali e il verde fanno di Le Corbusier un paesaggista innovatore; con la Ville Contemporaine per tre milioni di abitanti (1922), Il Plano Voisin per Parigi (1925), e la Ville Radieuse per un milione e mezzo di abitanti (1929), Le Corbusier trasforma in chiave contemporanea e dirompente le teorizzazioni di Howard sulla Città-Giardino, delineando in forma ipermoderna il nuovo rapporto tra natura e sistemi urbani, la cui qualità genera una totale integrazione tra artificio e natura, disciplinando altresí una nuova gerarchia negli spazi pubblici.Il saggio esplora esperienze di trasformazioni del paesaggio urbano.

Published
2018

49. Ombitasvir, paritaprevir, and ritonavir, with or without dasabuvir, plus ribavirin for patients with hepatitis C virus genotype 1 or 4 infection with cirrhosis (ABACUS): a prospective observational study

Author

Petta, Salvatore, Marzioni, Marco, Russo, Pierluigi, Aghemo, Alessio, Alberti, Alfredo, ASCIONE, ANTONIO, Antinori, Andrea, Bruno, Raffaele, Bruno, Savino, Chirianni, Antonio, Gaeta, Giovanni Battista, Giannini, Edoardo G, Merli, Manuela, Messina, Vincenzo, Montilla, Simona, Perno, Carlo Federico, Puoti, Massimo, Raimondo, Giovanni, Rendina, Maria, Silberstein, Francesca Ceccherini, Villa, Erica, Zignego, Anna Linda, Pani, Luca, Craxì, Antonio, Tabone, Marco, Andreoni, Massimo, Teti, Elisabetta, Angelico, Mario, Persico, Marcello, Masarone, Mario, Chiodera, Aledssandro, Solinas, Attilio, delle Monache, Marco, Cecere, Roberto, Maria Schimizzi, Anna, Piovesan, Sara, Campagnolo, Davide, Chiara Piras, Maria, Zolfino, Teresa, Paolo Russo, Francesca, Morelli, Olivia, Sangiovanni, Vincenzo, Onofrio, Mirella, Iodice, Valentina, Izzi, Antonio, Pirisi, Massimo, Danieli, Elena, Vinci, Maria, Rizzardini, Giuliano, Fagiuoli, Stefano, Pasulo, Luisa, D'Arminio Monforte, Antonella, Zuin, Massimo, Giorgini, Alessia, Simeone, Filomena, Piali, Guido, Lo Guercio, Carmela, Federico, Alessandro, Brancaccio, Giuseppina, Marrone, Aldo, Abbati, Giuseppe, Boarini, Chiara, Borghi, Vanni, Bernabucci, Veronica, Corti, Giampaolo, Monti, Monica, Rizzetto, Mario, Martini, Silvia, Andreone, Pietro, Gianstefani, Alice, Lenzi, Marco, Verucchi, Gabriella, Toniutto, Pierluigi, Borgia, Guglielmo, Caporaso, Nicola, Morisco, Filomena, Nardone, Gaetano, Angrisani, Debora, Giacometti, Andrea, Benedetti, Antonio, Tarantino, Giuseppe, Marsetti, Fabio, Tavio, Marcello, Novara, Sergio, Antonia Santantonio, Teresa, Serviddio, Gaetano, Brunetto, Maurizia, Coco, Barbara, Angarano, Gioacchino, Milella, Michele, BARONE, MICHELE, Di Leo, Alfredo, Ettore Sansonno, Domenico, Cacciola, Irene, Boffa, Nicola, Saracco, Giorgio, Di Biagio, Antonio, Picciotto, Antonino, de Luca, Andrea, Calvaruso, Vincenza, Corradori, Silvia, Ferrari, Carlo, Orlandini, Alessandra, Maida, Ivana, Torti, Carlo, Chessa, Luchino, Felder, Martina, Vespasiani Gentilucci, Umberto, Angeli, Paolo, Romano, Antonietta, Ludovico Rapaccini, Gian, Miele, Luca, Cima, Serena, Luisa Russo, Maria, Cozzolongo, Raffaele, Onnelli, Giovanna, D'Offizi, Giampiero, Lionetti, Raffaella, Montalbano, Marzia, Guerra, Michele, Di Perri, Giovanni, Boglione, Lucio, Capra, Franco, Carolo, Giada, Ieluzzi, Donatella, Antonini, Cinzia, Termite, Antonio, Madonia, Salvatore, Tarquini, Pierluigi, Parruti, Giustino, Vecchiet, Giacomo, Falasca, Katia, Menzaghi, Barbara, Quirino, Tiziana, Dentone, Chiara, Maria Piscaglia, Anna, Rossi, Cristina, Giordani, Maria, Fontanella, Luca, Cassola, Giovanni, Russello, Maurizio, Cristaudo, Antonio, Giacomet, Vania, Colombo, Massimo, Donato, Francesca, Durante, Emanuele, Cosco, Lucio, Marignani, Massimo, Quartini, Mariano, Memoli, Massimo, Ganga, Roberto, Ponti, Laura, Soria, Alessandro, Grazia Rumi, Maria, Gulminetti, Roberto, Maserati, Renato, Mondelli, Mario, Lazzarin, Adriano, Rita Parisi, Maria, Canovari, Benedetta, Avancini, Ivo, Pravadelli, Cecilia, Blanc, Pier, Pasquazzi, Caterina, Maria Mastroianni, Claudio, Lichtner, Myriam, Distefano, Marco, Magnani, Silvia, Paganin, Simona, Erne, Elke, Gatti, Pietro, Tundi, Paolo, Calabrese, Paolo, Gasbarrini, Antonio, Grieco, Antonio, Coppola, Nicola, Del Poggio, Paolo, Levrero, Massimo, Talliani, Gloria, Vullo, Vincenzo, Cauda, Roberto, La Monica, Silvia, Potenza, Domenico, Rizzo, Salvatore, Castelli, Francesco, Marie Pigozzi, Grazielle, Ciancio, Alessia, Romagnoli, Dante, Barchetti, Federica, Ivanovic, Jelena, Longo, Olimpia, Petraglia, Sandra, Paola Trotta, Maria, NARDONE, GERARDO ANTONIO PIO, Petta, Salvatore, Marzioni, Marco, Russo, Pierluigi, Aghemo, Alessio, Alberti, Alfredo, Ascione, Antonio, Antinori, Andrea, Bruno, Raffaele, Bruno, Savino, Chirianni, Antonio, Gaeta, Giovanni Battista, Giannini, Edoardo G, Merli, Manuela, Messina, Vincenzo, Montilla, Simona, Perno, Carlo Federico, Puoti, Massimo, Raimondo, Giovanni, Rendina, Maria, Silberstein, Francesca Ceccherini, Villa, Erica, Zignego, Anna Linda, Pani, Luca, Craxì, Antonio, Tabone, Marco, Andreoni, Massimo, Teti, Elisabetta, Angelico, Mario, Persico, Marcello, Masarone, Mario, Chiodera, Aledssandro, Solinas, Attilio, delle Monache, Marco, Cecere, Roberto, Maria Schimizzi, Anna, Piovesan, Sara, Campagnolo, Davide, Chiara Piras, Maria, Zolfino, Teresa, Paolo Russo, Francesca, Morelli, Olivia, Sangiovanni, Vincenzo, Onofrio, Mirella, Iodice, Valentina, Izzi, Antonio, Pirisi, Massimo, Danieli, Elena, Vinci, Maria, Rizzardini, Giuliano, Fagiuoli, Stefano, Pasulo, Luisa, D'Arminio Monforte, Antonella, Zuin, Massimo, Giorgini, Alessia, Simeone, Filomena, Piali, Guido, Lo Guercio, Carmela, Federico, Alessandro, Brancaccio, Giuseppina, Marrone, Aldo, Abbati, Giuseppe, Boarini, Chiara, Borghi, Vanni, Bernabucci, Veronica, Corti, Giampaolo, Monti, Monica, Rizzetto, Mario, Martini, Silvia, Andreone, Pietro, Gianstefani, Alice, Lenzi, Marco, Verucchi, Gabriella, Toniutto, Pierluigi, Borgia, Guglielmo, Caporaso, Nicola, Morisco, Filomena, Nardone, Gaetano, Angrisani, Debora, Giacometti, Andrea, Benedetti, Antonio, Tarantino, Giuseppe, Marsetti, Fabio, Tavio, Marcello, Novara, Sergio, Antonia Santantonio, Teresa, Serviddio, Gaetano, Brunetto, Maurizia, Coco, Barbara, Angarano, Gioacchino, Milella, Michele, Barone, Michele, Di Leo, Alfredo, Ettore Sansonno, Domenico, Cacciola, Irene, Boffa, Nicola, Saracco, Giorgio, Di Biagio, Antonio, Picciotto, Antonino, de Luca, Andrea, Calvaruso, Vincenza, Corradori, Silvia, Ferrari, Carlo, Orlandini, Alessandra, Maida, Ivana, Torti, Carlo, Chessa, Luchino, Felder, Martina, Vespasiani Gentilucci, Umberto, Angeli, Paolo, Romano, Antonietta, Ludovico Rapaccini, Gian, Miele, Luca, Cima, Serena, Luisa Russo, Maria, Cozzolongo, Raffaele, Onnelli, Giovanna, D'Offizi, Giampiero, Lionetti, Raffaella, Montalbano, Marzia, Guerra, Michele, Di Perri, Giovanni, Boglione, Lucio, Capra, Franco, Carolo, Giada, Ieluzzi, Donatella, Antonini, Cinzia, Termite, Antonio, Madonia, Salvatore, Tarquini, Pierluigi, Parruti, Giustino, Vecchiet, Giacomo, Falasca, Katia, Menzaghi, Barbara, Quirino, Tiziana, Dentone, Chiara, Maria Piscaglia, Anna, Rossi, Cristina, Giordani, Maria, Fontanella, Luca, Cassola, Giovanni, Russello, Maurizio, Cristaudo, Antonio, Giacomet, Vania, Colombo, Massimo, Donato, Francesca, Durante, Emanuele, Cosco, Lucio, Marignani, Massimo, Quartini, Mariano, Memoli, Massimo, Ganga, Roberto, Ponti, Laura, Soria, Alessandro, Grazia Rumi, Maria, Gulminetti, Roberto, Maserati, Renato, Mondelli, Mario, Lazzarin, Adriano, Rita Parisi, Maria, Canovari, Benedetta, Avancini, Ivo, Pravadelli, Cecilia, Blanc, Pier, Pasquazzi, Caterina, Maria Mastroianni, Claudio, Lichtner, Myriam, Distefano, Marco, Magnani, Silvia, Paganin, Simona, Erne, Elke, Gatti, Pietro, Tundi, Paolo, Calabrese, Paolo, Gasbarrini, Antonio, Grieco, Antonio, Coppola, Nicola, Del Poggio, Paolo, Levrero, Massimo, Talliani, Gloria, Vullo, Vincenzo, Cauda, Roberto, La Monica, Silvia, Potenza, Domenico, Rizzo, Salvatore, Castelli, Francesco, Marie Pigozzi, Grazielle, Ciancio, Alessia, Romagnoli, Dante, Barchetti, Federica, Ivanovic, Jelena, Longo, Olimpia, Petraglia, Sandra, Paola Trotta, Maria, Petta, S., Marzioni, M., Russo, P., Aghemo, A., Alberti, A., Ascione, A., Antinori, A., Bruno, R., Bruno, S., Chirianni, A., Gaeta, G., Giannini, E., Merli, M., Messina, V., Montilla, S., Perno, C., Puoti, M., Raimondo, G., Rendina, M., Silberstein, F., Villa, E., Zignego, A., Pani, L., Craxi, A., Tabone, M., Andreoni, M., Teti, E., Angelico, M., Persico, M., Masarone, M., Chiodera, A., Solinas, A., delle Monache, M., Cecere, R., Maria Schimizzi, A., Piovesan, S., Campagnolo, D., Chiara Piras, M., Zolfino, T., Paolo Russo, F., Morelli, O., Sangiovanni, V., Onofrio, M., Iodice, V., Izzi, A., Pirisi, M., Danieli, E., Vinci, M., Rizzardini, G., Fagiuoli, S., Pasulo, L., D'Arminio Monforte, A., Zuin, M., Giorgini, A., Simeone, F., Piali, G., Lo Guercio, C., Federico, A., Brancaccio, G., Marrone, A., Abbati, G., Boarini, C., Borghi, V., Bernabucci, V., Corti, G., Monti, M., Rizzetto, M., Martini, S., Andreone, P., Gianstefani, A., Lenzi, M., Verucchi, G., Toniutto, P., Borgia, G., Caporaso, N., Morisco, F., Nardone, G., Angrisani, D., Giacometti, A., Benedetti, A., Tarantino, G., Marsetti, F., Tavio, M., Novara, S., Antonia Santantonio, T., Serviddio, G., Brunetto, M., Coco, B., Angarano, G., Milella, M., Barone, M., Di Leo, A., Ettore Sansonno, D., Cacciola, I., Boffa, N., Saracco, G., Di Biagio, A., Picciotto, A., de Luca, A., Calvaruso, V., Corradori, S., Ferrari, C., Orlandini, A., Maida, I., Torti, C., Chessa, L., Felder, M., Vespasiani Gentilucci, U., Angeli, P., Romano, A., Ludovico Rapaccini, G., Miele, L., Cima, S., Luisa Russo, M., Cozzolongo, R., Onnelli, G., D'Offizi, G., Lionetti, R., Montalbano, M., Guerra, M., Di Perri, G., Boglione, L., Capra, F., Carolo, G., Ieluzzi, D., Antonini, C., Termite, A., Madonia, S., Tarquini, P., Parruti, G., Vecchiet, G., Falasca, K., Menzaghi, B., Quirino, T., Dentone, C., Maria Piscaglia, A., Rossi, C., Giordani, M., Fontanella, L., Cassola, G., Russello, M., Cristaudo, A., Giacomet, V., Colombo, M., Donato, F., Durante, E., Cosco, L., Marignani, M., Quartini, M., Memoli, M., Ganga, R., Ponti, L., Soria, A., Grazia Rumi, M., Gulminetti, R., Maserati, R., Mondelli, M., Lazzarin, A., Rita Parisi, M., Canovari, B., Avancini, I., Pravadelli, C., Blanc, P., Pasquazzi, C., Maria Mastroianni, C., Lichtner, M., Distefano, M., Magnani, S., Paganin, S., Erne, E., Gatti, P., Tundi, P., Calabrese, P., Gasbarrini, A., Grieco, A., Coppola, N., Del Poggio, P., Levrero, M., Talliani, G., Vullo, V., Cauda, R., La Monica, S., Potenza, D., Rizzo, S., Castelli, F., Marie Pigozzi, G., Ciancio, A., Romagnoli, D., Barchetti, F., Ivanovic, J., Longo, O., Petraglia, S., Paola Trotta, M., Savino, Bruno, Nardone, GERARDO ANTONIO PIO, Petta, S, Marzioni, M, Russo, P, Aghemo, A, Alberti, A, Ascione, A, Antinori, A, Bruno, R, Bruno, S, Chirianni, A, Gaeta, G, Giannini, E, Merli, M, Messina, V, Montilla, S, Perno, C, Puoti, M, Raimondo, G, Rendina, M, Silberstein, F, Villa, E, Zignego, A, Pani, L, Craxì, A, and Fagiuoli, S

Subjects
Cyclopropanes, Compassionate Use Trials, Liver Cirrhosis, Male, chemistry.chemical_compound, 0302 clinical medicine, 2-Naphthylamine, HCV, direct-acting antiviral, mixed cryoglobulinemia, RBV, Anilides, 030212 general & internal medicine, Longitudinal Studies, Prospective Studies, Chronic, Adult, Aged, Antiviral Agents, Carbamates, Drug Therapy, Combination, Female, Genotype, Hepatitis C, Chronic, Humans, Macrocyclic Compounds, Middle Aged, Ribavirin, Ritonavir, Sulfonamides, Treatment Outcome, Uracil, Settore MED/12 - Gastroenterologia, Dasabuvir, HCV DAA, Gastroenterology, virus diseases, Valine, Settore MED/07 - Microbiologia e Microbiologia Clinica, Hepatitis C, Hepatology, Combination, 030211 gastroenterology & hepatology, medicine.drug, medicine.medical_specialty, Proline, Lactams, Macrocyclic, Hepatitis C virus genotype 1, Hepatitis C virus genotype 4, decompensated liver cirrhosis, antiviral therapy, dasabuvir, ombitasvir, paritaprevir, 03 medical and health sciences, Drug Therapy, Internal medicine, medicine, Decompensation, Adverse effect, business.industry, Virology, Ombitasvir, Clinical trial, chemistry, Paritaprevir, business
Abstract

Summary Background We ran a compassionate use nationwide programme (ABACUS) to provide access to ombitasvir, paritaprevir, and ritonavir, with dasabuvir, plus ribavirin for hepatitis C virus (HCV) genotype 1 infection and ombitasvir, paritaprevir, and ritonavir, plus ribavirin for HCV genotype 4 infection in patients with cirrhosis at high risk of decompensation while approval of these regimens was pending in Italy. Methods In this prospective observational study, we collected data from a compassionate use nationwide programme from March 17, 2014, to May 28, 2015. Patients with HCV genotype 1 infection and cirrhosis at high risk of decompensation were given coformulated ombitasvir (25 mg), paritaprevir (150 mg), and ritonavir (100 mg) once daily and dasabuvir (250 mg) twice daily for 12 weeks (patients with HCV genotype 1b infection) or 24 weeks (patients with HCV genotype 1a infection). Patients with HCV genotype 4 infection were given coformulated ombitasvir (25 mg), paritaprevir (150 mg), and ritonavir (100 mg) once per day for 24 weeks. All patients were given weight-based ribavirin. The primary efficacy endpoint was sustained virological response at week 12 after the end of treatment (SVR12), analysed by intention-to-treat. Univariate and multivariate logistic regression analyses were used to identify baseline characteristics associated with SVR12. Adverse events were recorded throughout the study. Findings 728 (96%) of 762 patients with cirrhosis who were given ombitasvir, paritaprevir, and ritonavir, with or without dasabuvir, plus ribavirin therapy for 12 or 24 weeks achieved SVR12. Logistic regression analyses identified that bilirubin concentrations of less than 2 mg/dL were associated with SVR12 (odds ratio [OR] 4·76 [95% CI 1·83–12·3]; p=0·001). 166 (23%) of 734 patients included in safety analyses had an adverse event. 25 (3%) patients discontinued treatment because of adverse events. Asthenia was the most commonly reported adverse event, occurring in 36 (5%) patients. Interpretation Our findings suggest that the safety and effectiveness of ombitasvir, paritaprevir, and ritonavir, with or without dasabuvir, plus ribavirin in patients with HCV genotype 1 or 4 infection and cirrhosis at high risk of decompensation in a real-life setting are similar to those reported in clinical trials. The concordance with clinical trials provides reassurance that the reported efficacy of this treatment in clinical trials will translate to its use in routine clinical practice. Funding Dipartimento Biomedico di Medicina Interna e Specialistica dell'Universita di Palermo.

Published
2017

50. The landscape of strongholds

Author

LEONE, Manfredi, Longo, O, Ferlenga, A, Iorio, A, Sigurtà, D, Orsini, F, Dalzero, S, Taylor, W, Nulli, A, Condello, A, Albrecht, B, Battaino, C, and Leone, M

Subjects
Paesaggio, guerra, trasformazioni, difese, muraglia, canali, fortezze, fortificazioni, canali, linee d'acqua, Settore ICAR/15 - Architettura Del Paesaggio, Landscape, strongholds, waterline, Great Wall, Netherlands, transformations, land, ground, borders
Abstract

La difesa dei territori ha offerto risultati straordinari alla formazione e alla trasformazione del territorio e del paesaggio, a volte con tratti spettacolari e dimensioni incredibili. La celebrazione del centenario dell’inizio della Prima guerra mondiale ha offerto lo spunto per una riflessione sulle ricadute indotte sul paesaggio dagli eventi bellici, e più in generale dalla realizzazione di strutture difensive e del loro rapporto con il paesaggio circostante. Territorial defence led to extraordinary results in terms of forming and transforming the territory and landscape, at times with spectacular sights and incredible sizes. The one hundred year anniversary since the beginning of the First World War offered a hint for reflecting on the spin-offs on the landscape, caused by the war and more in general by the defensive structures and their relation with the surrounding landscape.

Published
2016

Catalog

Books, media, physical & digital resources
See catalog results