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3. Collagen IVα345 dysfunction in glomerular basement membrane diseases. I. Discovery of a COL4A3 variant in familial Goodpasture’s and Alport diseases

Author

Pokidysheva, Elena N., Seeger, Harald, Pedchenko, Vadim, Chetyrkin, Sergei, Bergmann, Carsten, Abrahamson, Dale, Cui, Zhao Wei, Delpire, Eric, Fervenza, Fernando C., Fidler, Aaron L., Fogo, Agnes B., Gaspert, Ariana, Grohmann, Maik, Gross, Oliver, Haddad, George, Harris, Raymond C., Kashtan, Clifford, Kitching, A. Richard, Lorenzen, Johan M., McAdoo, Stephen, Pusey, Charles D., Segelmark, Marten, Simmons, Alicia, Voziyan, Paul A., Wagner, Timo, Wüthrich, Rudolf P., Zhao, Ming-Hui, Boudko, Sergei P., Kistler, Andreas D., and Hudson, Billy G.

Published
2021
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10. Therapeutic miR-21 Silencing Ameliorates Diabetic Kidney Disease in Mice

Author

Kölling, Malte, Kaucsar, Tamas, Schauerte, Celina, Hübner, Anika, Dettling, Angela, Park, Joon-Keun, Busch, Martin, Wulff, Xaver, Meier, Matthias, Scherf, Kristian, Bukosza, Nóra, Szénási, Gábor, Godó, Mária, Sharma, Amit, Heuser, Michael, Hamar, Peter, Bang, Claudia, Haller, Hermann, Thum, Thomas, and Lorenzen, Johan M.

Published
2017
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11. Altered glycosylation of IgG4 promotes lectin complement pathway activation in anti-PLA2R1-associated membranous nephropathy

Author

Haddad, George, Lorenzen, Johan M., Ma, Hong, de Haan, Noortje, Seeger, Harald, Zaghrini, Christelle, Brandt, Simone, Kolling, Malte, Wegmann, Urs, Kiss, Bence, Pal, Gabor, Gal, Peter, Wuthrich, Rudolf P., Wuhrer, Manfred, Beck, Laurence H., Salant, David J., Lambeau, Gerard, and Kistler, Andreas D.

Subjects
Care and treatment, Development and progression, Genetic aspects, Health aspects, Cell receptors -- Health aspects, Lectins -- Health aspects, Kidney diseases -- Genetic aspects -- Development and progression -- Care and treatment, Gene expression -- Health aspects
Abstract

Introduction Primary membranous nephropathy (pMN) is an organ-specific autoimmune kidney disease caused by circulating autoantibodies that target surface antigens on glomerular podocytes (1-3). The M-type phospholipase A2 receptor (PLA2R1) is [...], Primary membranous nephropathy (pMN) is a leading cause of nephrotic syndrome in adults. In most cases, this autoimmune kidney disease is associated with autoantibodies against the M-type phospholipase A2 receptor (PLA2R1) expressed on kidney podocytes, but the mechanisms leading to glomerular damage remain elusive. Here, we developed a cell culture model using human podocytes and found that anti-PLA2R1-positive pMN patient sera or isolated IgG4, but not IgG4-depleted sera, induced proteolysis of the 2 essential podocyte proteins synaptopodin and NEPH1 in the presence of complement, resulting in perturbations of the podocyte cytoskeleton. Specific blockade of the lectin pathway prevented degradation of synaptopodin and NEPH1. Anti-PLA2R1 IgG4 directly bound mannose-binding lectin in a glycosylation-dependent manner. In a cohort of pMN patients, we identified increased levels of galactose-deficient IgG4, which correlated with anti-PLA2R1 titers and podocyte damage induced by patient sera. Assembly of the terminal C5b-9 complement complex and activation of the complement receptors C3aR1 or C5aR1 were required to induce proteolysis of synaptopodin and NEPH1 by 2 distinct proteolytic pathways mediated by cysteine and aspartic proteinases, respectively. Together, these results demonstrated a mechanism by which aberrantly glycosylated IgG4 activated the lectin pathway and induced podocyte injury in primary membranous nephropathy.

Published
2021
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18. Renal AAV2-Mediated Overexpression of Long Non-Coding RNA H19 Attenuates Ischemic Acute Kidney Injury Through Sponging of microRNA-30a-5p

Author

Haddad, George, Kölling, Malte, Wegmann, Urs A, Dettling, Angela, Seeger, Harald, Schmitt, Roland, Soerensen-Zender, Inga, Haller, Hermann, Kistler, Andreas D, Dueck, Anne, Engelhardt, Stefan, Thum, Thomas, Mueller, Thomas F, Wüthrich, Rudolf P, Lorenzen, Johan M, University of Zurich, Lorenzen, Johan M, and Publica

Subjects
2727 Nephrology, 610 Medicine & health, 10035 Clinic for Nephrology
Abstract

Background. Renal ischemia-reperfusion (I/R) injury is a major cause of AKI. Noncoding RNAs are intricately involved in the pathophysiology of this form of AKI. Transcription of hypoxia-induced, long noncoding RNA H19, which shows high embryonic expression and is silenced in adults, is upregulated in renal I/R injury. Methods. Lentivirus-mediated overexpression, as well as antisense oligonucleotide-based silencing, modulated H19 in vitro. In vivo analyses used constitutive H19 knockout mice. In addition, renal vein injection of adeno-associated virus 2 (AAV2) carrying H19 caused overexpression in the kidney. Expression of H19 in kidney transplant patients with I/R injury was investigated. Results. H19 is upregulated in kidney biopsies of patients with AKI, in murine ischemic kidney tissue, and in cultured and ex vivo sorted hypoxic endothelial cells (ECs) and tubular epithelial cells (TECs). Transcription factors hypoxia-inducible factor 1-a, LHX8, and SPI1 activate H19 in ECs and TECs. H19 overexpression promotes angiogenesis in vitro and in vivo. In vivo, transient AAV2-mediated H19 overexpression significantly improved kidney function, reduced apoptosis, and reduced inflammation, as well as preserving capillary density and tubular epithelial integrity. Sponging of miR-30a-5p mediated the effects, which, in turn, led to target regulation of Dll4, ATG5, and Snai1. Conclusions. H19 overexpression confers protection against renal injury by stimulating proangiogenic signaling. H19 overexpression may be a promising future therapeutic option in the treatment of patients with ischemic AKI.

Published
2021

20. Osteopontin is indispensible for AP1-mediated angiotensin II-related miR-21 transcription during cardiac fibrosis

Author

Lorenzen, Johan M., Schauerte, Celina, Hübner, Anika, Kölling, Malte, Martino, Filippo, Scherf, Kristian, Batkai, Sandor, Zimmer, Karina, Foinquinos, Ariana, Kaucsar, Tamas, Fiedler, Jan, Kumarswamy, Regalla, Bang, Claudia, Hartmann, Dorothee, Gupta, Shashi K., Kielstein, Jan, Jungmann, Andreas, Katus, Hugo A., Weidemann, Frank, Müller, Oliver J., Haller, Hermann, and Thum, Thomas

Published
2015
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22. Prevalence and correlates of gout in a large cohort of patients with chronic kidney disease: the German Chronic Kidney Disease (GCKD) study

Author

Jing, Jiaojiao, Kielstein, Jan T., Schultheiss, Ulla T., Sitter, Thomas, Titze, Stephanie I., Schaeffner, Elke S., McAdams-DeMarco, Mara, Kronenberg, Florian, Eckardt, Kai-Uwe, Köttgen, Anna, Eckardt, Kai-Uwe, Titze, Stephanie, Prokosch, Hans-Ulrich, Bärthlein, Barbara, Reis, André, Ekici, Arif B., Gefeller, Olaf, Hilgers, Karl F., Hübner, Silvia, Avendaño, Susanne, Becker-Grosspitsch, Dinah, Hauck, Nina, Seuchter, Susanne A., Hausknecht, Birgit, Rittmeier, Marion, Weigel, Anke, Beck, Andreas, Ganslandt, Thomas, Knispel, Sabine, Dressel, Thomas, Malzer, Martina, Floege, Jürgen, Eitner, Frank, Schlieper, Georg, Findeisen, Katharina, Arweiler, Elfriede, Ernst, Sabine, Unger, Mario, Lipski, Stefan, Schaeffner, Elke, Baid-Agrawal, Seema, Petzold, Kerstin, Schindler, Ralf, Köttgen, Anna, Schultheiss, Ulla, Meder, Simone, Mitsch, Erna, Reinhard, Ursula, Walz, Gerd, Haller, Hermann, Lorenzen, Johan, Kielstein, Jan T., Otto, Petra, Sommerer, Claudia, Föllinger, Claudia, Zeier, Martin, Wolf, Gunter, Busch, Martin, Paul, Katharina, Dittrich, Lisett, Sitter, Thomas, Hilge, Robert, Blank, Claudia, Wanner, Christoph, Krane, Vera, Schmiedeke, Daniel, Toncar, Sebastian, Cavitt, Daniela, Schönowsky, Karina, Börner-Klein, Antje, Kronenberg, Florian, Raschenberger, Julia, Kollerits, Barbara, Forer, Lukas, Schönherr, Sebastian, Weiensteiner, Hansi, Oefner, Peter, Gronwald, Wolfram, Zacharias, Helena, and Schmid, Matthias

Published
2015
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23. Disease burden and risk profile in referred patients with moderate chronic kidney disease: composition of the German Chronic Kidney Disease (GCKD) cohort

Author

Titze, Stephanie, Schmid, Matthias, Köttgen, Anna, Busch, Martin, Floege, Jürgen, Wanner, Christoph, Kronenberg, Florian, Eckardt, Kai-Uwe, Eckardt, Kai-Uwe, Titze, Stephanie, Prokosch, Hans-Ulrich, Bärthlein, Barbara, Beck, Andreas, Ganslandt, Thomas, Gefeller, Olaf, Schmid, Matthias, Köster, Jan, Malzer, Martina, Schlieper, Georg, Eitner, Frank, Meisen, Sabine, Kehl, Katharina, Arweiler, Elfriede, Floege, Jürgen, Schaeffner, Elke, Baid-Agrawal, Seema, Schindler, Ralf, Titze, Stephanie, Hübner, Silvia, Dienemann, Thomas, Hilgers, Karl F., Eckardt, Kai-Uwe, Köttgen, Anna, Schulthei, Ulla, Walz, Gerd, Kielstein, Jan T., Lorenzen, Johan, Haller, Hermann, Sommerer, Claudia, Zeier, Martin, Busch, Martin, Paul, Katharina, Wolf, Gunter, Hilge, Robert, Sitter, Thomas, Krane, Vera, Schmiedeke, Daniel, Toncar, Sebastian, Wanner, Christoph, Ekici, Arif B., Reis, André, Forer, Lukas, Schönherr, Sebastian, Weissensteiner, Hansi, Kollertits, Barbara, Raschenberger, Julia, Kronenberg, Florian, Gronwald, Wolfram, Zacharias, Helena, and Oefner, Peter

Published
2015
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29. Vascular importance of the miR-212/132 cluster

Author

Kumarswamy, Regalla, Volkmann, Ingo, Beermann, Julia, Napp, Lars Christian, Jabs, Olga, Bhayadia, Raj, Melk, Anette, Ucar, Ahmet, Chowdhury, Kamal, Lorenzen, Johan M., Gupta, Shashi Kumar, Batkai, Sandor, and Thum, Thomas

Published
2014
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32. TISSUE INJURY AND REPAIR

Author

Martino, Filippo, Kielstein, Jan, Bang, Claudia, Thum, Thomas, and Lorenzen, Johan

Published
2014

33. Safety of Kidney Biopsy when Performed as an Outpatient Procedure

Author

Bonani, Marco, Seeger, Harald; https://orcid.org/0000-0003-1552-7983, Weber, Nina, Lorenzen, Johan M; https://orcid.org/0000-0002-9132-948X, Wüthrich, Rudolf P; https://orcid.org/0000-0002-5425-6453, Kistler, Andreas D, Bonani, Marco, Seeger, Harald; https://orcid.org/0000-0003-1552-7983, Weber, Nina, Lorenzen, Johan M; https://orcid.org/0000-0002-9132-948X, Wüthrich, Rudolf P; https://orcid.org/0000-0002-5425-6453, and Kistler, Andreas D

Abstract

INTRODUCTION Kidney biopsy remains the gold standard for the diagnosis of most renal diseases. A major obstacle to performing a biopsy is safety concerns. However, many safety measures are not evidence based and therefore vary widely between centers. We sought to determine the rate and timing of kidney biopsy complications in our center, to compare the complication rate between native and transplant kidney biopsies, to evaluate the feasibility of performing kidney biopsies as an outpatient procedure and the value of a postbiopsy ultrasound before discharge, and to identify risk factors for complications. METHODS We performed a single-center, retrospective, observational study at the Division of Nephrology of the University Hospital Zurich including all patients who underwent renal biopsy between January 2005 and December 2017. Major bleeding (primary outcome) and any other bleeding or nonbleeding complications (secondary outcomes) were compared between native and transplant kidney biopsies and between inpatient and outpatient procedures and correlated with clinical factors possibly affecting bleeding risk. RESULTS Overall, 2,239 biopsies were performed in 1,468 patients, 732 as inpatient and 1,507 as outpatient procedures. Major bleeding was observed in 28 (3.8%) inpatient and in 15 (1.0%) outpatient procedures, totaling to 43 (1.9%) of all biopsies. Major bleeding requiring intervention amounted to 1.0% (0.5% of outpatient procedures). Rate of major bleeding was similar between native and transplant kidneys. 13/15 (87%) bleeding episodes in planned outpatient procedures were detected during the 4-h surveillance period. Risk factors for bleeding were aspirin use, low eGFR, anemia, cirrhosis, and amyloidosis. Routine postbiopsy ultrasound did not change management. CONCLUSIONS Kidney biopsy is an overall safe procedure and can be performed as an outpatient procedure in most patients with an observation period as short as 4 h. The value of routine postbiopsy ultrasoun

Published
2021

35. Renal AAV2-Mediated Overexpression of Long Non-Coding RNA H19 Attenuates Ischemic Acute Kidney Injury Through Sponging of microRNA-30a-5p

Author

Haddad, George, primary, Kölling, Malte, additional, Wegmann, Urs A., additional, Dettling, Angela, additional, Seeger, Harald, additional, Schmitt, Roland, additional, Soerensen-Zender, Inga, additional, Haller, Hermann, additional, Kistler, Andreas D., additional, Dueck, Anne, additional, Engelhardt, Stefan, additional, Thum, Thomas, additional, Mueller, Thomas F., additional, Wüthrich, Rudolf P., additional, and Lorenzen, Johan M., additional

Published
2021
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48. The Circular RNA Predicts Survival in Critically Ill Patients With Acute Kidney Injury

Author

Kölling, Malte, Seeger, Harald, Haddad, George, Kistler, Andreas, Nowak, Albina, Faulhaber-Walter, Robert, Kielstein, Jan, Haller, Hermann, Fliser, Danilo, Mueller, Thomas, Wüthrich, Rudolf P, Lorenzen, Johan M, University of Zurich, and Lorenzen, Johan M

Subjects
2727 Nephrology, 10265 Clinic for Endocrinology and Diabetology, 610 Medicine & health, 10035 Clinic for Nephrology, 2731 Ophthalmology
Published
2018
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50. Circular RNA-based biomarkers in blood of patients with Fabry disease and related phenotypes.

Author

Nowak, Albina, Haddad, George, Kistler, Andreas D., Nlandu-Khodo, Stellor, Beuschlein, Felix, Wüthrich, Rudolf P., Lorenzen, Johan M., and Kölling, Malte

Abstract

Background Fabry disease is a rare X-linked lysosomal storage disease caused by mutations in the galactosidase a gene. Deficient activity of a-galactosidase A leads to glycosphingolipid accumulations in multiple organs. Circular RNAs represent strong regulators of gene expression. Their circular structure ensures high stability in blood. We hypothesised that blood-based circular RNA profiles improve phenotypic assignment and therapeutic monitoring of Fabry disease. Methods A genome-wide circular RNA expression analysis was performed in blood of genetically diagnosed patients with Fabry disease (n=58), age-matched and sex-matched healthy volunteers (n=14) and disease control patients with acute kidney injury (n=109). Most highly dysregulated circular RNAs were validated by quantitative real-time PCR. Circular RNA biomarker sensitivity, specificity, predictive values and area under the curve (AUC) were determined. Linear regression analyses were conducted for validated circular RNA biomarkers and clinical patient characteristics. Results A distinct circular RNA transcriptome signature identified patients with Fabry disease. Level of circular RNAs hsa_circ_0006853 (AUC=0.73), hsa_circ_0083766 (AUC=0.8) and hsa_circ_0002397 (AUC=0.8) distinguished patients with Fabry disease from both healthy controls and patients with acute kidney injury. Hsa_circ_0002397 was, furthermore, female-specifically expressed. Circular RNA level were significantly related to galactosidase a gene mutations, early symptoms, phenotypes, disease severities, specific therapies and long-term complications of Fabry disease. Conclusion The discovery of circular RNA-based and Fabry disease-specific biomarkers may advance future diagnosis of Fabry disease and help to distinguish related phenotypes. [ABSTRACT FROM AUTHOR]

Published
2022
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