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In orthognathic surgery, patient-specific osteosynthesis implants (PSIs) represent a novel approach for the reproduction of the virtual surgical planning on the patient. The aim of this study is to analyse the quality of maxillo-mandibular positioning using a hybrid mandible-first mandibular-PSI-guided procedure on twenty-two patients while the upper maxilla was fixed using manually bent stock titanium miniplates. The virtual surgical plan was used to design PSIs and positioning guides, which were then 3D printed using biocompatible materials. A Cone Beam Computed Tomography (CBCT) scan was performed one month after surgery and postoperative facial skeletal models were segmented for comparison against the surgical plan. A three-dimensional cephalometric analysis was carried out on both planned and obtained anatomies. A Spearman correlation matrix was computed on the calculated discrepancies in order to achieve a more comprehensive description of maxillo-mandibular displacement. Intraoperatively, all PSIs were successfully applied. The procedure was found to be accurate in planned maxillo-mandibular positioning reproduction, while maintaining a degree of flexibility to allow for aesthetics-based verticality correction in a pitch range between -5.31 and +1.79 mm. Such a correction did not significantly affect the achievement of planned frontal symmetry.

Congenital abnormalities of the kidney and urinary tract are among the most common birth defects, affecting 3% of newborns. The human kidney forms around a million nephrons from a pool of nephron progenitors over a 30-week period of development. To establish a framework for human nephrogenesis, we spatially resolved a stereotypical process by which equipotent nephron progenitors generate a nephron anlage, then applied data-driven approaches to construct three-dimensional protein maps on anatomical models of the nephrogenic program. Single-cell RNA sequencing identified progenitor states, which were spatially mapped to the nephron anatomy, enabling the generation of functional gene networks predicting interactions within and between nephron cell types. Network mining identified known developmental disease genes and predicted targets of interest. The spatially resolved nephrogenic program made available through the Human Nephrogenesis Atlas (https://sckidney.flatironinstitute.org/) will facilitate an understanding of kidney development and disease and enhance efforts to generate new kidney structures., Competing Interests: Declaration of interests A.P.M. is a scientific advisor on kidney research at Novartis, TRESTLE Therapeutics, eGENESIS, and IVIVA Medical., (Copyright © 2021 Elsevier Inc. All rights reserved.)

In orthognathic surgery, the use of patient-specific osteosynthesis devices is a novel approach used to transfer the virtual surgical plan to the patient. The aim of this study is to analyse the quality of mandibular anatomy reproduction using a mandible-first mandibular-PSI guided procedure on 22 patients. Three different positioning guide designs were compared in terms of osteosynthesis plate positioning and mandibular anatomical outcome. PSIs and positioning guides were designed according to virtual surgical plan and 3D printed using biocompatible materials. A CBCT scan was performed 1 month after surgery and postoperative mandibular models were segmented for comparison against the surgical plan. A precision comparison was carried out among the three groups. Correlations between obtained rami and plates discrepancies and between planned rami displacements and obtained rami discrepancies were calculated. Intraoperatively, all PSIs were successfully applied. The procedure was found to be accurate in planned mandibular anatomy reproduction. Different guide designs did not differ in mandibular outcome precision. Plate positional discrepancies influenced the corresponding ramus position, mainly in roll angle and vertical translation. Ramus planned displacement was found to be a further potential source of inaccuracy, possibly due to osteosynthesis surface interference.

Spike sorting is a crucial step in electrophysiological studies of neuronal activity. While many spike sorting packages are available, there is little consensus about which are most accurate under different experimental conditions. SpikeForest is an open-source and reproducible software suite that benchmarks the performance of automated spike sorting algorithms across an extensive, curated database of ground-truth electrophysiological recordings, displaying results interactively on a continuously-updating website. With contributions from eleven laboratories, our database currently comprises 650 recordings (1.3 TB total size) with around 35,000 ground-truth units. These data include paired intracellular/extracellular recordings and state-of-the-art simulated recordings. Ten of the most popular spike sorting codes are wrapped in a Python package and evaluated on a compute cluster using an automated pipeline. SpikeForest documents community progress in automated spike sorting, and guides neuroscientists to an optimal choice of sorter and parameters for a wide range of probes and brain regions., Competing Interests: JM, JJ, EL, AM, CH, AB, SG, AB No competing interests declared, (© 2020, Magland et al.)

Purpose: We developed an innovative computer-assisted method to increase the accuracy of the surgery-first (SF) approach by linking the virtual orthodontic planning (VOP) with the virtual surgical planning (VSP)., Materials and Methods: Fifteen consecutive patients were enrolled from 2013 to 2015. All 15 patients had initially undergone cone-beam computed tomography (CBCT; 15 × 15 field-of-view) and intraoral digital scanning of the dental arches. The DICOM (Digital Imaging and Communications in Medicine) data set and STL files were processed using the SimPlant O&O platform (Dentsply-Sirona, York, PA), which facilitates skeletal, dental, and soft tissue modeling and subsequent realization of the VOP/VSP. The VSP was reproduced using computer-aided design and computer-aided manufacturing surgical splints, and the VOP was realized via postoperative orthodontic treatment. At the end of treatment, all the patients underwent repeat CBCT and digital scanning of the dental arches, and the new data sets were compared with the original data sets to determine the deviations. To evaluate skeletal accuracy, we assessed all points within an arbitrary range of -2 to +2 mm. To evaluate dental accuracy, the arbitrary range was -0.8 to +0.8 mm., Results: The average duration of orthodontic treatment was 17.9 months. The accuracy of maxillary treatment averaged 0.0702 ± 2.0724 mm and that of mandibular treatment, 0.2811 ± 1.9993 mm. The average upper and lower dental arch accuracy was -0.0029 ± 1.125 and -0.0147 ± 1.263 mm, respectively. The maxillary surgery accuracy was 75.3% and that of mandibular surgery 74.0%, both within the -2 to +2-mm range. The upper and lower arch accuracy was 58.86 and 51.53%, respectively, both within the -0.8 to +0.8-mm range., Conclusions: The use of the VOP/VSP improved the diagnostic and therapeutic SF preoperative planning. VOP contributed significantly in this context. The accuracy of skeletal repositioning was acceptable; however, the VSP should be rendered more reproducibly in the future to minimize the need for orthodontic compensation and to maximize the advantages of SF., (Copyright © 2019 American Association of Oral and Maxillofacial Surgeons. Published by Elsevier Inc. All rights reserved.)