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386 results on '"Loving, Crystal L."'

13. Integrative profiling of gene expression and chromatin accessibility elucidates specific transcriptional networks in porcine neutrophils

Author

Herrera-Uribe, Juber, primary, Lim, Kyu-Sang, additional, Byrne, Kristen A., additional, Daharsh, Lance, additional, Liu, Haibo, additional, Corbett, Ryan J., additional, Marco, Gianna, additional, Schroyen, Martine, additional, Koltes, James E., additional, Loving, Crystal L., additional, and Tuggle, Christopher K., additional

Published
2023
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26. Additional file 1 of Effects of β-glucan on Salmonella enterica serovar Typhimurium swine colonization and microbiota alterations

Author

Bearson, Shawn M. D., Trachsel, Julian M., Bearson, Bradley L., Loving, Crystal L., Kerr, Brian J., Shippy, Daniel C., and Kiros, Tadele G.

Abstract

Additional file 1. Fig. S1: A comparison of fecal alpha diversity (Shannon index) between the diets over time. NC=no β-glucan control pig group; BG= β-glucan-fed pig group. F0=prior to in-feed β-glucan; D0=prior to Salmonella inoculation; D2, D7, D16= 2, 7 and 16 days post-inoculation with Salmonella.

Published
2023
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31. Evaluation of digestively resistant or soluble fibers, short- and medium-chain fatty acids, trace minerals, and antibiotics in nonchallenged nursery pigs on performance, digestibility, and intestinal integrity

Author

Kerr, Brian J, primary, Trachsel, Julian M, additional, Bearson, Bradley L, additional, Loving, Crystal L, additional, Bearson, Shawn M D, additional, Byrne, Kristen A, additional, Pearce, Sarah C, additional, Ramirez, Shelby M, additional, Gabler, Nicholas K, additional, Schweer, Wesley P, additional, Helm, Emma T, additional, and De Mille, Carson M, additional

Published
2022
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38. Evaluation of digestively resistant or soluble fibers, short- and medium-chain fatty acids, trace minerals, and antibiotics in nonchallenged nursery pigs on performance, digestibility, and intestinal integrity

Author

Kerr, Brian J., Trachsel, Julian M., Bearson, Bradley L., Loving, Crystal L., Bearson, Shawn M. D., Byrne, Kristen A., Pearce, Sarah C., Ramirez, Shelby M., Gabler, Nicholas K., Schweer, Wesley P., Helm, Emma T., De Mille, Carson M., Kerr, Brian J., Trachsel, Julian M., Bearson, Bradley L., Loving, Crystal L., Bearson, Shawn M. D., Byrne, Kristen A., Pearce, Sarah C., Ramirez, Shelby M., Gabler, Nicholas K., Schweer, Wesley P., Helm, Emma T., and De Mille, Carson M.

Abstract

Nonantibiotic in-feed additives have limited effects on performance, nutrient digestibility, and intestinal function when supplemented to diets fed to nursery pigs. Lay Summary In-feed antimicrobials have been an important technology in swine production for protecting health and supporting growth. However, with legislative restrictions on the use of most antibiotics for growth promotion, research is needed to evaluate in-feed additives in replacing this growth promoting technology. Thus, strategies to enhance energy and nutrient digestibility, intestinal function and integrity, gastrointestinal volatile fatty acid concentrations, and microbial ecology in nursery pigs are desirable targets. The results of the three experiments conducted herein do not indicate that supplementing diets with digestively resistant but fermentable fibers, short-medium-chain fatty acids, or antibiotics have a consistent positive or negative effect on markers of intestinal integrity or barrier function, VFA patterns (ileal, cecal, or colon), ATTD of energy and nutrients, or pig performance. Three experiments (EXP) were conducted to determine the effect of feed additives on performance, intestinal integrity, gastrointestinal volatile fatty acids (VFA), and energy and nutrient digestion in nonchallenged nursery pigs. In EXP 1, 480 pigs (6.36-kg body weight, BW) were placed into 96 pens with 5 pigs/pen, and allotted to 1 of 10 dietary treatments: 1) negative control containing no feed additive (NC), 2) NC + 44 mg chlortetracycline and 38.5 mg tiamulin/kg diet (CTsb), 3) NC + 5% resistant potato starch (RSpo), 4) NC + 5% soluble corn fiber (SCF), 5) NC + 5% sugar beet pulp (SBP), 6) NC + 0.30% fatty acid mix (FAM), 7) NC + 0.10% phytogenic blend of essential oils and flavoring compounds (PHY), 8) NC + 50 mg Cu and 1,600 mg zinc oxide/kg diet (CuZn), 9) NC + 5% resistant corn starch (RScn), and 10) NC + 0.05% beta-glucan (BG) for 28 d. There was no impact of dietary treatment on BW gain or feed

Published
2022

41. Additional file 5 of Assessment of DNA methylation in porcine immune cells reveals novel regulatory elements associated with cell-specific gene expression and immune capacity traits

Author

Corbett, Ryan J., Luttman, Andrea M., Herrera-Uribe, Juber, Liu, Haibo, Raney, Nancy E., Grabowski, Jenna M., Loving, Crystal L., Tuggle, Christopher K., and Ernst, Catherine W.

Abstract

Additional file 5: Figure S1. Associations between immune cell global methylation and DNA methyltransferase (DNMT) expression. Moderate negative and positive correlations were observed between global methylation and normalized transcript abundance of DNMT1 (A) and DNMT3A (B), respectively. Normalized DNMT3B abundance was significantly negatively correlated with global DNA methylation (C). Considering abundance of all DNMTs, the normalized abundances of DNMT1 and DNMT3B subtracted from DNMT3A abundance were significantly positively correlated with global methylation (D). Figure S2. Methylation rates across immune cell marker genes CD19 (A), SIRPA (B), and TBX21 (C), and CD4 (D) for expressing and non-expressing cell types. Gray boxes indicate cell differentially methylated regions (cDMRs) within each gene locus, and black dots indicate CpG coordinates. Figure S3. (A) Dot plot of average methylation at CD8A TSS-proximal cell differentially methylated regions (cDMRs). (B) Dot plot of CD8A transcript abundance as percentage of maximum transcripts per kilobase million (TPM) across samples. Letters indicate statistically significant differences between means (p < 0.05). Figure S4. Positive methylation:expression correlations are associated with more lowly expressed genes and highly methylated regions. (A) Transcripts per kilobase million (TPM) of genes overlapping cDMRs negatively and positively correlated with gene expression, separated by gene feature. (B) Methylation levels of cDMRs negatively and positively correlated with gene expression, separated by gene feature. **=p

Published
2022
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43. Regional epithelial cell diversity in the small intestine of pigs.

Author

Wiarda, Jayne E, Becker, Sage R, Sivasankaran, Sathesh K, and Loving, Crystal L

Subjects
EPITHELIAL cells, GENE expression, SMALL intestine, RNA analysis, ENTEROCYTES, RNA sequencing
Abstract

Understanding regional distribution and specialization of small intestinal epithelial cells is crucial for developing methods to control appetite, stress, and nutrient uptake in swine. To establish a better understanding of specific epithelial cells found across different regions of the small intestine in pigs, we utilized single-cell RNA sequencing (scRNA-seq) to recover and analyze epithelial cells from duodenum, jejunum, and ileum. Cells identified included crypt cells, enterocytes, BEST4 enterocytes, goblet cells, and enteroendocrine (EE) cells. EE cells were divided into two subsets based on the level of expression of the EE lineage commitment gene, NEUROD1. NEUROD1 hi EE cells had minimal expression of hormone-encoding genes and were dissimilar to EE cells in humans and mice, indicating a subset of EE cells unique to pigs. Recently discovered BEST4 enterocytes were detected in both crypts and villi throughout the small intestine via in situ staining, unlike in humans, where BEST4 enterocytes are found only in small intestinal villi. Proximal-to-distal gradients of expression were noted for hormone-encoding genes in EE cells and nutrient transport genes in enterocytes via scRNA-seq, demonstrating regional specialization. Regional gene expression in EE cells and enterocytes was validated via quantitative PCR (qPCR) analysis of RNA isolated from epithelial cells of different small intestinal locations. Though many genes had similar patterns of regional expression when assessed by qPCR of total epithelial cells, some regional expression was only detected via scRNA-seq, highlighting advantages of scRNA-seq to deconvolute cell type-specific regional gene expression when compared to analysis of bulk samples. Overall, results provide new information on regional localization and transcriptional profiles of epithelial cells in the pig small intestine. [ABSTRACT FROM AUTHOR]

Published
2023
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50. Development and characterization of new swine immune reagents to understand immune correlates for vaccines, infection, and biomedical research outcomes

Author

Gourapura, Renukaradhya J, primary, Loving, Crystal L, additional, Kenney, Scott, additional, LaBresh, Joanna, additional, Patil, Veerupaxagouda, additional, Byrne, Kristen, additional, Walker, Kristen, additional, Dai, Chaohui, additional, Hailstock, Taylor, additional, and Lunney, Joan K, additional

Published
2021
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