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Your search keyword '"Lu, Chien-Hung"' showing total 43 results
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43 results on '"Lu, Chien-Hung"'

2. Mutations in the PKM2 exon-10 region are associated with reduced allostery and increased nuclear translocation

Author

Chen, Tsan-Jan, Wang, Hung-Jung, Liu, Jai-Shin, Cheng, Hsin-Hung, Hsu, Sheng-Chieh, Wu, Meng-Chen, Lu, Chien-Hung, Wu, Yu-Fang, Wu, Jing-Wen, Liu, Ying-Yuan, Kung, Hsing-Jien, and Wang, Wen-Ching

Subjects
Cancer, Genetics, Aetiology, 2.1 Biological and endogenous factors, Active Transport, Cell Nucleus, Allosteric Regulation, Carrier Proteins, Exons, Histone Demethylases, Humans, Immunohistochemistry, Membrane Proteins, Models, Molecular, Mutation, Neoplasms, Protein Conformation, Thyroid Hormones
Abstract

PKM2 is a key metabolic enzyme central to glucose metabolism and energy expenditure. Multiple stimuli regulate PKM2's activity through allosteric modulation and post-translational modifications. Furthermore, PKM2 can partner with KDM8, an oncogenic demethylase and enter the nucleus to serve as a HIF1α co-activator. Yet, the mechanistic basis of the exon-10 region in allosteric regulation and nuclear translocation remains unclear. Here, we determined the crystal structures and kinetic coupling constants of exon-10 tumor-related mutants (H391Y and R399E), showing altered structural plasticity and reduced allostery. Immunoprecipitation analysis revealed increased interaction with KDM8 for H391Y, R399E, and G415R. We also found a higher degree of HIF1α-mediated transactivation activity, particularly in the presence of KDM8. Furthermore, overexpression of PKM2 mutants significantly elevated cell growth and migration. Together, PKM2 exon-10 mutations lead to structure-allostery alterations and increased nuclear functions mediated by KDM8 in breast cancer cells. Targeting the PKM2-KDM8 complex may provide a potential therapeutic intervention.

Published
2019

3. Quantum Phase Imaging using Spatial Entanglement

Author

Lu, Chien-Hung, Reichert, Matthew, Sun, Xiaohang, and Fleischer, Jason W.

Subjects
Physics - Optics, Quantum Physics
Abstract

Entangled photons have the remarkable ability to be more sensitive to signal and less sensitive to noise than classical light. Joint photons can sample an object collectively, resulting in faster phase accumulation and higher spatial resolution, while common components of noise can be subtracted. Even more, they can accomplish this while physically separate, due to the nonlocal properties of quantum mechanics. Indeed, nearly all quantum optics experiments rely on this separation, using individual point detectors that are scanned to measure coincidence counts and correlations. Scanning, however, is tedious, time consuming, and ill-suited for imaging. Moreover, the separation of beam paths adds complexity to the system while reducing the number of photons available for sampling, and the multiplicity of detectors does not scale well for greater numbers of photons and higher orders of entanglement. We bypass all of these problems here by directly imaging collinear photon pairs with an electron-multiplying CCD camera. We show explicitly the benefits of quantum nonlocality by engineering the spatial entanglement of the illuminating photons and introduce a new method of correlation measurement by converting time-domain coincidence counting into spatial-domain detection of selected pixels. We show that classical transport-of-intensity methods are applicable in the quantum domain and experimentally demonstrate nearly optimal (Heisenberg-limited) phase measurement for the given quantum illumination. The methods show the power of direct imaging and hold much potential for more general types of quantum information processing and control.

Published
2015

15. Flow-Scanning Microfluidic Imaging

Author

Pégard, Nicolas, Lu, Chien-Hung, Toth, Marton, and Fleischer, Monica Driscoll and Jason

Subjects
GeneralLiterature_REFERENCE(e.g.,dictionaries,encyclopedias,glossaries)
Published
2016

38. GRPlacer

Author

Dai, Ke-Ren, primary, Lu, Chien-Hung, additional, and Li, Yih-Lang, additional

Published
2009
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43. Detecting small lung tumors in mouse models by refractive-index microradiology

Author

Chien, Chia-Chi, Zhang, Guilin, Hwu, Y., Liu, Ping, Yue, Weisheng, Sun, Jianqi, Li, Yan, Xue, Hongjie, Xu, Lisa X., Wang, Chang Hai, Chen, Nanyow, Lu, Chien Hung, Lee, Ting-Kuo, Yang, Yuh-Cheng, Lu, Yen-Ta, Ching, Yu-Tai, Shih, T. F., Yang, P. C., Je, J. H., and Margaritondo, G.

Subjects
Real-time imaging, Nodules, Carcinoma, Coronary-Angiography, Synchrotron X-ray imaging, CIBM-PC, X-Ray Source, Lung cancer, Synchrotron-Radiation, Radiology, Tomography, Cancer, Ct, Mammography
Abstract

Refractive-index (phase-contrast) radiology was able to detect lung tumors less than 1 mm in live mice. Significant micromorphology differences were observed in the microradiographs between normal, inflamed, and lung cancer tissues. This was made possible by the high phase contrast and by the fast image taking that reduces the motion blur. The detection of cancer and inflammation areas by phase contrast microradiology and microtomography was validated by bioluminescence and histopathological analysis. The smallest tumor detected is less than 1 mm(3) with accuracy better than 1 x 10(-3) mm(3). This level of performance is currently suitable for animal studies, while further developments are required for clinical application.

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