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Your search keyword '"Lu, Hai-Qin"' showing total 28 results
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28 results on '"Lu, Hai-Qin"'

23. The Flavonoid Biosynthesis and Regulation in Brassica napus : A Review.

Author

Chen, Yuan-Yuan, Lu, Hai-Qin, Jiang, Kai-Xuan, Wang, Yi-Ran, Wang, You-Ping, and Jiang, Jin-Jin

Subjects
*RAPESEED, *EDIBLE fats & oils, *FOOD of animal origin, *RUTABAGA, *METABOLITES, *URBAN tourism, *BIOSYNTHESIS, *FLAVONOIDS
Abstract

Brassica napus is an important crop for edible oil, vegetables, biofuel, and animal food. It is also an ornamental crop for its various petal colors. Flavonoids are a group of secondary metabolites with antioxidant activities and medicinal values, and are important to plant pigmentation, disease resistance, and abiotic stress responses. The yellow seed coat, purple leaf and inflorescence, and colorful petals of B. napus have been bred for improved nutritional value, tourism and city ornamentation. The putative loci and genes regulating flavonoid biosynthesis in B. napus have been identified using germplasms with various seed, petal, leaf, and stem colors, or different flavonoid contents under stress conditions. This review introduces the advances of flavonoid profiling, biosynthesis, and regulation during development and stress responses of B. napus, and hopes to help with the breeding of B. napus with better quality, ornamental value, and stress resistances. [ABSTRACT FROM AUTHOR]

Published
2023
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28. [Construction and screening of hepatitis E virus-specific phage antibody combinatorial library].

Author

Wei H, Zhang JQ, Lu HQ, Meng JH, Lu XX, and Xie W

Subjects
Humans, Immunoglobulin kappa-Chains genetics, Polymerase Chain Reaction, Hepatitis Antibodies genetics, Hepatitis E virus immunology, Peptide Library, Viral Proteins immunology
Abstract

Aim: To construct HEV-specific phage combinatorial anti-body library and screen anti-HEV antibodies with neutralizing activity from the library., Methods: The total RNA was extracted from B-lymphocytes of 6 HE patients. Kappa chain and Fd segment of IgG gene were amplified respectively by RT-PCR using a set of Fab-specific primers. The amplified gene were inserted successively into vector pComb3 and electrotransformed E. coli XLI-Blue cells. Furthermore, the recombinant phage was rescued by being concultured with helper phage VCSM13 to construct HEV-specific phage anti-body library., Results: Fab displayed on the surface a as fusion protein with the N terminal of coat protein III, and 1. 8 x 10(7) clone library was established. Specific antibodies to HEV ORF2 recombinant antigen were acquired after five rounds of panning with HEV ORF2 recombinant antigen including neutralizing epitope., Conclusion: Four clones exhibited specific binding to HEV ORF2 recombinant antigen including neutralizing epitope is identified by ELISA. The results show that we have got the recombinant phage antibodies.

Published
2003

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