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1. Synthesis of precipitating chromogenic/fluorogenic β-glucosidase/β-galactosidase substrates by a new method and their application in the visual detection of foodborne pathogenic bacteria.

Author

Wei, Xianhu, Zhang, Youxiong, Wu, Qingping, Zhang, Jumei, Guo, Weipeng, Chen, Moutong, Gu, Qihui, Cai, Zhihe, and Lu, Mianfei

Subjects
*GLUCOSIDASE synthesis, *FOOD pathogens, *PATHOGENIC bacteria
Abstract

We developed a new efficient method for the synthesis of important indoxyl glycoside substrates for β-glucosidase and β-galactosidase by using 1-acetylindol-3-ones as intermediates. This method was used to synthesise novel precipitating fluorogenic substrates for β-glucosidase based on 2-(benzothiazol-2′-yl)-phenols. We also assessed the application of these substrates in the detection of foodborne pathogenic bacteria. [ABSTRACT FROM AUTHOR]

Published
2017
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